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471.
To assess the impact of aquaculture activities, we measured the primary production, total sediment oxygen consumption, and benthic nutrient flux at two aquaculture farms (sea squirt and oyster) and one reference site. The estimated primary production in the water column ranged from 19–169 mmol C m−2 d−1. Total sediment oxygen consumption rates ranged from 58 to 328 mmol m−2 d−1, 1.1 to 40 mmol m−2 d−1 for nitrogen, 0.17 to 3.0 mmol m−2 d−1 for phosphate, and 7.3 to 74 mmol m−2 d−1 for silicate. The average total sediment oxygen consumption at the longline farms was >2.5 times higher than the reference site. Nitrate was significantly removed by denitrification at the longline farms in July and September and ranged from −5.4 to −0.09 mmol m−2 d−1, which is higher than for other coastal sediments. The benthic fluxes of nitrogen and phosphate at longline farms were up to 16 and six times higher than at the reference site, respectively. The average nitrogen requirements of the primary producers were 9.3 mmol m−2 d−1 at the sea squirt farm, 7.0 mmol m−2 d−1 at the oyster farm, and 13.5 mmol m−2 d−1 at the reference site, corresponding to 88, 316, and 27.2% of the nitrogen supplied by benthic fluxes, respectively. Our results suggest that benthic nutrient fluxes at longline farms are a major nutrient source for primary production in coastal waters.  相似文献   
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Insects are a potential source of proteins and fats which can be incorporated into diets of broiler chickens. Accordingly, black soldier fly larvae oil (BSFLO) needs to be tested as an appropriate fat source to produce healthy chickens for consumers. Therefore, the objective of the present study was to evaluate the effects of the replacement of soybean oil (SBO) with BSFLO in broiler diets on intestinal health and blood profiles. A total of 210 one-day-old male broilers were randomly allocated to three dietary treatments (10 replicates of seven birds per group): a control diet and two experimental diets in which SBO was replaced with 50% (50 BSFLO) or 100% (100 BSFLO) BSFLO. At the end of the study (35 days), 18 birds (six broilers per treatment) were slaughtered to determine the intestinal morphology, digestibility, and volatile fatty acid (VFA) profile. Blood samples were collected from 24 randomly selected birds (eight broilers per treatment) to determine the blood profiles. BSFLO supplementation positively affected villus height but did not affect digestibility. BSFLO showed no adverse effects on the VFA and blood profiles. In conclusion, the results of this study suggest that SBO can be replaced by BSFLO without any adverse effects on broiler health.  相似文献   
475.
ABSTRACT

High global production of medium-density fiberboard (MDF) in recent years could generate an equal quantity of waste MDF at the end of its service life, requiring recycling of waste MDF instead of landfilling or incineration. This study investigated effects of the addition of recycled fiber (RF) obtained from surface laminated MDFs with three different materials to the properties of three-layer recycled MDF (rMDF). Three types of surface laminates such as low-pressure laminate, polyethylene terephthalate, and polyester coating were hammer milled, and then went through a patent-pending fiber recovery system to obtain the resultant RFs that were added to the core layer of rMDF. These RFs at three contents (10, 20, and 30%) were blended with 12% of urea-formaldehyde (UF) resin prior to hot-pressing. Statistical analysis showed that the best internal bonding strength, modulus of rupture, and modulus of elasticity of rMDF panels were obtained for LPL-rMDF with a 20% RF content. Thickness swelling, water absorption, and formaldehyde emission of rMDF were reduced by increasing the RF content. These findings suggest that a minimum RF content of 20% can be replaced with virgin fibers for the rMDF manufacture, indicating the feasibility of recycling waste laminated MDF into three-layer rMDF.  相似文献   
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The genus Edwardsiella is one of the major causes of fish diseases globally. Herein, we examined 37 isolates from ten different fish species from India, South Korea and Taiwan to gain insight into their phenotypic and genotypic properties, of which 30 were characterized as E. tarda with phenotypic homology estimated at 85.71% based on API‐20E biochemical tests. Genotyping using 16S rRNA put all isolates together with E. anguillarum, E. hoshinae, E. tarda, E. piscicida and E. ictaluri reference strains in a monophyletic group. In contrast, the gyrB phylogenetic tree clearly separated E. ictaluri, E. tarda and E. hoshinae reference strains from our isolates and put our isolates into two groups with group I being homologous with the E. anguillarum reference strain while group II was homologous with the E. piscicida reference strain. Hence, our findings point to E. piscicida and E. anguillarum as species infecting different fish species in Asia. Homology of the ompW protein suggested that strains with broad protective coverage could be identified as vaccine candidates. This study underscores the importance of combining genotyping with phenotyping for valid species classification. In addition, it accentuates the importance of phylogenetic comparison of bacterial antigens for identification of potential vaccine candidates.  相似文献   
478.
The Korean Food Code has approved irradiation less than 7 kGy for microbial control in algal food, which is often not sufficient to achieve the acceptable contamination level (<104 CFU/g). In this study, heat‐assisted low‐dose electron beam (E‐beam) irradiation was applied for improving the microbial quality of dried laver through the response surface optimization of process conditions (Xn); irradiation dose (0–4 kGy, X1), heating temperature (140–180°C, X2) and heating time (0–28 s, X3) for their effects on total aerobic bacteria (TAB) count, moisture content, chlorophyll content, carotenoid content and overall palatability of dried laver. TAB counts were affected more by irradiation dose than by heating temperature and time. Moisture, chlorophyll and carotenoid content and overall palatability were more affected by heating temperature and heating time than by irradiation dose. The overall results indicated that the optimal conditions were irradiation dose of 1.8–3.0 kGy, heating temperature of 154–170°C and heating time of 10–18 s to achieve appropriate quality attributes of heat‐assisted E‐beam irradiated dried laver, such as TAB count of 103 CFU/g, moisture content of 5% and 6.5 of good overall palatability score out of 7.  相似文献   
479.
Flours obtained by a specific polishing process were used to prepare sourdough and bread. Three fractions designated C‐1 (100–90%), C‐5 (60–50%), and C‐8 (30–0%) were studied. The pH, total titratable acidity levels, and buffering capacity of sourdoughs made from polished flours were significantly different from those of the control sourdough with No. 1 Canada Western Red Spring (CW), and they provided sourdough breads with better qualities than that of CW. The growth of lactic acid bacteria and yeast in polished flour sourdoughs were significantly accelerated during fermentation over that in CW sourdough. Higher maturation of polished flour sourdoughs softened the hardness of mixed dough. The intricate network of honeycomb structure gluten and uneven surface of starch granules were distinctly observed in SEM images. Substitutions of C‐5 or C‐8 sourdoughs for CW significantly increased the loaf volume and softened breadcrumbs more than CW sourdough. Flour qualities of polished flours such as suitable acidity and good buffering capacity caused by the bran fraction were effective for better growth and longer life of yeast in the dough during fermentation. Therefore, application of polished flours in sourdough bread would improve rheological properties of dough and bread as compared with CW sourdough.  相似文献   
480.
为探讨5-氟尿嘧啶的药效、毒性与给药剂量之间的关系,采用BALB/c小鼠来源的CT26结肠癌细胞株构建小鼠结肠癌模型,分别给予10、20、30和50 mg/kg剂量的5-氟尿嘧啶,分析其抗肿瘤效果、对小鼠体质量的影响和死亡率等指标.结果表明,5-氟尿嘧啶的体内抑瘤效果及毒性与剂量密切相关.综合分析评价各指标,认为20~30 mg/kg剂量的5-氟尿嘧啶较适合于小鼠结肠癌模型中的药效评价.  相似文献   
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