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31.
Five outbreaks of myocarditis were investigated in young sheep. They occurred during late winter and spring when there was lush growth of pasture following a prolonged period of drought. Clinically the disease was characterised by sudden death and pathological findings were dominated by acute multifocal locally extensive necrotising and haemorrhagic myocarditis. A fluorescent antibody technique was used to demonstrate the presence of Clostridium chauvoei in paraffin embedded sections of myocardium from 4 of the outbreaks. 相似文献
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Canary grass (Phalaris sp) toxicosis in sheep in California 总被引:1,自引:0,他引:1
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Investigations were conducted on several small neighbouring beef cattle and sheep farms that were found to be contaminated with polychlorinated biphenyls (PCBs). The residues detected contained about 62% w/w chlorine and soil concentrations ranged from 0.35 to 1.9 mg/kg. Beef cattle and sheep grazing the contaminated land had PCB concentrations in their fat of 0.3 to 1.7 mg/kg and 0.19 to 0.45 mg/kg, respectively. The concentration of PCBs in the caudal fat of cattle was 0.3 to 2 times the concentration of PCBs in the land they were grazing and was positively related to stocking rates. PCBs were present in milk fat from cattle at about the same concentration as in caudal fat, and lactation appeared to contribute to decontamination. Wool grease from sheep contained about 1 to 2 times the concentration of PCBs in subcutaneous fat. Calves of contaminated cows also became contaminated. The half-life for decontamination of PCBs in a group of 8 young female beef cattle was calculated at 13 months. PCBs were detected in pasture samples at concentrations ranging from less than 0.01 mg/kg to 0.12 mg/kg. However, PCBs were not detected in any sample of hay cut from these paddocks. In 4 sheep fed a sole ration of this hay, traces of PCBs were detected in their fat following 89 days of feeding. Grazing livestock for meat production on land contaminated with PCBs is not recommended because livestock readily acquire residues, and PCBs persist in soil and livestock for long periods. Grazing sheep for wool production on land contaminated with PCBs may be an option. 相似文献
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Molecular cloning and expression of FGF2 gene in pre‐implantation developmental stages of in vitro‐produced sheep embryos 下载免费PDF全文
Early embryonic mortality is one of the main sources of reproductive loss in domestic ruminants including sheep. Fibroblast growth factor‐2 (FGF‐2) is a member of FGFs family that mediates trophoblast activities and regulates embryonic development in various species. In this study, we have cloned, characterized sheep FGF2 cDNA (KU316368) and studied the expression in sheep embryos. Ovaries of non‐pregnant sheep were collected from local abattoir and matured in culture medium at 38.5ºC, 5% CO2, 95% humidity for 22–24 hr. The matured oocytes were inseminated with capacitated spermatozoa in Brackett and Oliphant medium and resulted embryos were cultured in CO2 incubator for 6–7 days to complete the developmental stages from two cells to blastocyst stage. Total RNA was extracted from immature oocytes (n = 100), mature oocytes (n = 100) and different stages of embryos such as 2 cell (n = 50), 4 cell (n = 25), 8 cell (n = 12), 16 cell (n = 6), morula (n = 5) and blastocyst (n = 3). The total RNA isolated from the oocytes and embryos was reverse transcribed and subjected to real‐time polymerase chain reaction using sequence‐specific primers and SYBR green as the DNA dye. On sequence analysis, the nucleotide sequence of sheep FGF2 exhibited highest sequence similarity with cattle (100%) and least with rat and mouse (69.2%). At the deduced amino acid level, a highest degree of similarity was noticed with cattle, buffalo, goat, pig, camel and horse (100%) and lowest degree of identity with rat, human and mouse (98.2%). The FGF2 mRNA expression was higher in immature and mature oocytes and gradually decreases from 2‐cell stage of embryo to the blastocyst stage. More over a significant differences in FGF2 mRNA expression (p < .05) were observed between immature oocytes and all pre‐implantation stages of embryo. It can be concluded that FGF‐2 plays a significant role in pre‐implantation and early development of embryos in sheep. 相似文献
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East EM 《Science (New York, N.Y.)》1909,29(742):465-467
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1饲料工业与品牌策略“WTO”与机遇今年11月15日与美国的谈判结束后,中国入关指日可成。21对世纪中国将进一步加快全球经济一体化的进程,针对新的竞争环境,饲料工业企业必须适应新的情况调整战略,除了密切注意原料价格的行情,及时调整产品结构,提高内部的管理水准,还要建立起一个有生命力的品牌,良好的品牌与良好的外在形象将是饲料企业市场竞争取胜的法宝。只要企业具备市场竞争力,加入WTO将是企业取得发展的极好机会。用强势品牌来控制市场品牌不是简单的产品加商标,品牌是指社会大众对企业及其产品的认同与忠诚。… 相似文献
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可持续发展定义为:既满足当代人的需要,又不对后代人满足其需要的能力构成危害的发展.森林资源可持续发展的含义:无退化地使用林地;维持多种生态效益;保护森林生态系统内生物的多样性;产品的生产不能超过其再生能力,对森林多种用途的综合管理以满足后代的需求.山区以林为主的方针,应处理好开展与治理的关系和经济效益与生态效益的关系. 相似文献