全文获取类型
收费全文 | 419篇 |
免费 | 56篇 |
专业分类
林业 | 15篇 |
农学 | 2篇 |
41篇 | |
综合类 | 73篇 |
农作物 | 14篇 |
水产渔业 | 27篇 |
畜牧兽医 | 276篇 |
园艺 | 15篇 |
植物保护 | 12篇 |
出版年
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 11篇 |
2019年 | 6篇 |
2018年 | 5篇 |
2017年 | 15篇 |
2016年 | 6篇 |
2015年 | 12篇 |
2014年 | 16篇 |
2013年 | 19篇 |
2012年 | 22篇 |
2011年 | 17篇 |
2010年 | 6篇 |
2009年 | 6篇 |
2008年 | 23篇 |
2007年 | 22篇 |
2006年 | 21篇 |
2005年 | 18篇 |
2004年 | 23篇 |
2003年 | 24篇 |
2002年 | 13篇 |
2001年 | 8篇 |
2000年 | 10篇 |
1999年 | 13篇 |
1998年 | 5篇 |
1995年 | 5篇 |
1994年 | 3篇 |
1992年 | 3篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 7篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1981年 | 3篇 |
1980年 | 7篇 |
1979年 | 4篇 |
1974年 | 7篇 |
1972年 | 4篇 |
1971年 | 6篇 |
1970年 | 4篇 |
1969年 | 6篇 |
1968年 | 4篇 |
1967年 | 5篇 |
1966年 | 6篇 |
1962年 | 3篇 |
1960年 | 4篇 |
1928年 | 3篇 |
1912年 | 2篇 |
排序方式: 共有475条查询结果,搜索用时 31 毫秒
51.
Much has been gained from genomic and evolutionary studies of species. Combining the perspectives of these different approaches suggests that an integrated phylogenomic approach will be beneficial. 相似文献
52.
The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin 总被引:1,自引:0,他引:1
Nagano T Mitchell JA Sanz LA Pauler FM Ferguson-Smith AC Feil R Fraser P 《Science (New York, N.Y.)》2008,322(5908):1717-1720
53.
Seshadri R Adrian L Fouts DE Eisen JA Phillippy AM Methe BA Ward NL Nelson WC Deboy RT Khouri HM Kolonay JF Dodson RJ Daugherty SC Brinkac LM Sullivan SA Madupu R Nelson KE Kang KH Impraim M Tran K Robinson JM Forberger HA Fraser CM Zinder SH Heidelberg JF 《Science (New York, N.Y.)》2005,307(5706):105-108
54.
HistoryA 2-year-old, entire female, Somali cat weighing 3.8 kg was admitted for a conjunctival graft on the right eye, for treatment of an acute descemetocele. Medetomidine 4.2 μg kg?1 and methadone 0.2 mg kg?1 were administered by intramuscular injection as preanaesthetic medication. Anaesthesia was induced using diazepam 0.26 mg kg?1 and propofol 4 mg kg?1 administered by intravenous (IV) injection. Following endotracheal intubation, anaesthesia was maintained with isoflurane delivered in oxygen (1 L minute?1) and nitrous oxide (2 L minute?1) via a non-rebreathing system. Twenty minutes after induction of anaesthesia, one drop of a 10% phenylephrine hydrochloride solution was administered topically to the right eye.Physical examinationAfter phenylephrine administration, a decrease in heart rate (from 95 to 80 beats minute?1) and an increase in arterial blood pressure occurred. The pulse then became difficult to palpate manually and multifocal ventricular premature contractions were observed on the electrocardiogram.ManagementNitrous oxide was discontinued and the isoflurane vaporizer setting was decreased from 1.5% to 0.5%. Lidocaine 1 mg kg?1 IV was administered, this resulted in ventricular bigeminy. The quality of the femoral pulse improved and was regular in rhythm and character. Surgery was completed as fast as possible. The bigeminy progressively disappeared and before disconnecting the cat from the breathing system, there was a normal sinus rhythm with a heart rate of 85 beats minute?1.Follow-upEchocardiography was performed during recovery and showed mitral and aortic valve insufficiency and dilation of the left ventricle, suggesting a reduction in systolic function. Echocardiography was repeated the following day and was normal.ConclusionsIn order to diminish the potential for cardiovascular sequelae associated with systemic absorption of ocular phenylephrine, less concentrated solutions, smaller drop size or different instillation techniques should be considered for topical use in small patients. 相似文献
55.
56.
Orwell Ralph L. Wood Ronald L. Tarran Jane Torpy Fraser Burchett Margaret D. 《Water, air, and soil pollution》2004,157(1-4):193-207
Water, Air, &; Soil Pollution - The quality of the indoor environment has become a major health consideration, since urban-dwellers spend 80-90% of their time indoors, where air pollution can be... 相似文献
57.
58.
59.
Plasmid transfer and plasmid-mediated genetic exchange in Brucella abortus. 总被引:2,自引:0,他引:2 下载免费PDF全文
Naturally-occurring plasmids and gene transfer mechanisms have not yet been reported in brucellae. Here we show that Brucella abortus is capable of maintaining and transferring the broad-host-range plasmids pTH10 (IncP), pSa (IncW) and R751 (IncP), and describe pTH10-mediated transfer of B. abortus chromosomal genes to Escherichia coli. All three plasmids transferred by conjugation from E. coli to B. abortus S19, and from B. abortus S19 to B. abortus 292 (biovar 4). They were stably maintained with no effect on biotyping characteristics. Plasmid pTH10 is a Tn1-containing derivative of RP4. It confers temperature-sensitive resistance to kanamycin, tetracycline and ampicillin to E. coli, but its tetracycline resistance and temperature sensitivity were poorly expressed in B. abortus. Plasmids pTH10 and pSa both transferred from B. abortus to E. coli DP50, a strain that is auxotrophic for diaminopimelic acid (DAP) Plasmid pTH10 (but not pSa) mobilized Brucella chromosomal gene(s) for DAP synthesis to DP50, yielding non-DAP-requiring (NDR) transconjugants. Neither plasmid transferred the NDR marker from their original E. coli host strains, nor did pTH10 transfer it from NDR transconjugants. Escherichia coli NDR transconjugant EP8.11 was cured of pTH10 by passage at the nonpermissive temperature, but retained the NDR marker and the Tn1-encoded resistance to ampicillin, indicating Tn1-mediated integration of Brucella chromosomal DNA into the E. coli chromosome. 相似文献
60.