Sequence variation in the putative effector gene SIX8 facilitates molecular differentiation of Fusarium oxysporum f. sp. cubense     

S. Fraser‐Smith  E. Czislowski  R. A. Meldrum  M. Zander  W. O'Neill  G. R. Balali  E. A. B. Aitken 《Plant pathology》2014,63(5):1044-1052

Fusarium oxysporum f. sp. cubense (Foc), causal agent of fusarium wilt of banana, is among the most destructive pathogens of banana and plantain. The development of a molecular diagnostic capable of reliably distinguishing between the various races of the pathogen is of key importance to disease management. However, attempts to distinguish isolates using the standard molecular loci typically used for fungal phylogenetics have been complicated by a poor correlation between phylogeny and pathogenicity. Among the available alternative loci are several putative effector genes, known as SIX genes, which have been successfully used to differentiate the three races of F. oxysporum f. sp. lycopersici. In this study, an international collection of Foc isolates was screened for the presence of the putative effector SIX8. Using a PCR and sequencing approach, variation in Foc‐SIX8 was identified which allowed race 4 to be differentiated from race 1 and 2 isolates, and tropical and subtropical race 4 isolates to be distinguished from one another.  相似文献   

Uterine adenomyosis in an orang-utan (Pongo abelii/pygmaeus)     

KJ Graham  FA Hulst  L Vogelnest  IS Fraser  CM Shilton 《Australian veterinary journal》2009,87(1-2):66-69

A 48-year-old, multiparous, female hybrid orang-utan ( Pongo abelii/pygmaeus ) was investigated after a 3-year history of irregular and excessively heavy menstrual bleeding. Opportunistic pelvic examinations over a 2.5-year period were non-diagnostic. Medical therapy was not effective. A subtotal hysterectomy with bilateral salpingo-ovariectomy was performed. A pedunculated mass spanning 90% of the uterine lumen was seen grossly, and histopathology confirmed uterine adenomyosis. Adenomyosis is defined as the ectopic occurrence or diffuse implantation of endometrial tissue, including glands and stroma, into the myometrium. It is common in older, usually premenopausal, multiparous women and is frequently associated with other uterine pathology, including endometrial hyperplasia and leiomyomas. The most common clinical signs are dysmenorrhoea and heavy menstrual bleeding; however, up to 35% of women are asymptomatic. Diagnosis is difficult and requires myometrial sampling and an experienced pathologist. A hysterectomy in this case was diagnostic and curative. There have been few reports of uterine adenomyosis in non-human primates and none reported in an orang-utan. Uterine adenomyosis should be considered in the differential diagnosis in any multiparous, aged, non-human female primate with irregular and excessively heavy menstrual bleeding, and hysterectomy with bilateral salpingo-ovariectomy is recommended as a diagnostic and therapeutic solution.  相似文献   

Dental caries in the dog     

Fraser A. Hale 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(12):1301-1304

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Second order Horner's syndrome in a cat     

Luisa De Risio  James Fraser McConnell 《Journal of Feline Medicine and Surgery》2009,11(8):714-716

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A quantitative analysis of food movement convergence in four Canadian provinces     

Ashley McInnes  Evan Fraser  Ze’ev Gedalof  Jennifer Silver 《Agriculture and Human Values》2017,34(4):787-804

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Managing outbreaks of invasive species: Eradication versus suppression     

Rob W. Fraser  John D. Mumford  Andy Wilby  Jeff K. Waage 《国际虫害防治杂志》2013,59(4):261-268

Abstract

A framework is developed to evaluate eradication as one of three alternative management responses to an outbreak of an invasive species: eradication, suppression or no action. This framework can be used to establish under what conditions an eradication option could provide an expected net economic benefit, and whether this net benefit exceeds that of the other two options. The eradication option is more likely to be preferred in situations where there is an immediate export benefit that is derived from eradication of the outbreak, and also the uncertainty associated with the likely success of eradication is low.  相似文献   

A field comparison of conventional suckers with tissue culture banana planting material over three crop cycles     

J. C. Robinson  Connie Fraser  K. Eckstein 《The Journal of Horticultural Science and Biotechnology》2013,88(6):831-836

A randomized block split-plot experiment was established at Burgershall Research Station, eastern Transvaal to compare three cultivars (Williams, Dwarf Cavendish and Grand Nain) and three types of planting material (conventional 2 kg pared suckers, 200 and 500 mm high, tissue cultured plants rooted in bags). In the first crop cycle (P), tissue culture (TC) plants were up to 300 mm taller than conventional suckers (CON) at flowering. Over all three cultivars, TC plants yielded 56 t ha-1 annum“1 which was 20.4% higher than CON plants, due to larger bunches and a shorter cycle to harvest. Cvs Williams and Grand Nain yielded similarly at 54 t ha-1 annum-1 which was 9% more than ‘Dwarf Cavendish’ over all planting materials. There were 30 (1.76%) distinct somaclonal variants in the plantation of 1,700 plants. In the second crop cycle (Rl), there was a significant carry-over effect with TC plants, as shown by the increased vigour and size of the first ratoon followers selected on TC mother plants. Such TC followers outyielded CON followers by 18.6% in the second cycle, almost matching the first cycle. In the third crop cycle (R2) there was no difference in plant size between TC and CON, although a yield increase of 9.6% in favour of TC was obtained, indicating a reduced carry-over effect of TC by the third cycle. After three cycles, the average annual yield increase with TC was 19.4% for ‘Dwarf Cavendish’, 15% for ‘Williams’ and 13% for ‘Grand Nain’.  相似文献   

Intelligent estimation of spatially distributed soil physical properties     

Fabio Iwashita  Michael J. Friedel  Glaucielen F. Ribeiro  Stephen J. Fraser 《Geoderma》2012

Spatial analysis of soil samples is often times not possible when measurements are limited in number or clustered. To obviate potential problems, we propose a new approach based on the self-organizing map (SOM) technique. This approach exploits underlying nonlinear relation of the steady-state geomorphic concave–convex nature of hillslopes (from hilltop to bottom of the valley) to spatially limited soil textural data. The topographic features are extracted from Shuttle Radar Topographic Mission elevation data; whereas soil textural (clay, silt, and sand) and hydraulic data were collected in 29 spatially random locations (50 to 75 cm depth). In contrast to traditional principal component analysis, the SOM identifies relations among relief features, such as, slope, horizontal curvature and vertical curvature. Stochastic cross-validation indicates that the SOM is unbiased and provides a way to measure the magnitude of prediction uncertainty for all variables. The SOM cross-component plots of the soil texture reveals higher clay proportions at concave areas with convergent hydrological flux and lower proportions for convex areas with divergent flux. The sand ratio has an opposite pattern with higher values near the ridge and lower values near the valley. Silt has a trend similar to sand, although less pronounced. The relation between soil texture and concave–convex hillslope features reveals that subsurface weathering and transport is an important process that changed from loss-to-gain at the rectilinear hillslope point. These results illustrate that the SOM can be used to capture and predict nonlinear hillslope relations among relief, soil texture, and hydraulic conductivity data.  相似文献   

The effect of harvest date and inoculation on the yield, fermentation characteristics and feeding value of kale silage   总被引：3，自引：0，他引：3  

M. D. Fraser  A. Winters  R. Fychan  D. R. Davies  & R. Jones 《Grass and Forage Science》2001,56(2):151-161

Using new harvesting and ensiling technologies, it is now possible to ensile kale ( Brassica oleracea ) successfully. However, there is little information available regarding the optimum time for harvest. The aim of this 2-year study was to compare the yield, fermentation characteristics and feeding value of kale harvested at different stages of growth, and ensiled with and without a bacterial inoculant. During Year 1 the crop was harvested after 15, 18 and 20 weeks of growth. The yield at each harvest was similar, but as the crop matured the crude protein (CP) concentration and buffering capacity decreased significantly, and there was a marked increase in the water-soluble carbohydrate (WSC) concentration. During Year 2 the crop was harvested at 14 and 17 weeks of growth. Again, the stage of maturity did not affect yield and, on this occasion, the chemical composition of the crop was unaffected by harvest date. However, the WSC and CP concentrations of the crop were lower in Year 2 than in Year 1, possibly as a result of differences in fertilizer regime. Each of the silages produced in Year 2 was offered to six Suffolk cross wether lambs, aged 10 months, to measure voluntary intake, in vivo digestibility and nitrogen retention. Neither harvesting date nor the use of an inoculant affected voluntary intake or nitrogen retention by the lambs. However, in vivo digestibility was higher in the kale silage harvested after 14 weeks of growth and when an inoculant was applied. The results obtained suggest that harvesting kale after 14 weeks of growth can produce highly digestible silage with a high CP concentration. Although delaying harvest until 18 weeks of growth will probably result in a decrease in the CP concentration of the crop, it should also lead to an increase in the WSC concentration of the crop, ensuring a more reliable fermentation.  相似文献   

Cannabis intoxication in three Green iguanas (Iguana iguana)     

Girling SJ  Fraser MA 《The Journal of small animal practice》2011,52(2):113-116

This report describes clinical signs and plasma biochemical changes associated with significant cannabis consumption in three Green iguanas (Iguana iguana) which resulted in seizures, cardiovascular and digestive tract aberrations, elevated hepatic enzymes and bile acid concentrations for a number of weeks post recovery. One case required extensive antiseizuring therapy to recover. All Green iguanas eventually made a full recovery.  相似文献