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21.
The efficacy of an agent with an iodine-based active ingredient (a.i.) was evaluated for controlling the growth of fungi pathogenic to many different food crops. Even though iodine is a necessary mineral for mammals and is an approved food additive, interest in using iodine-based agrochemicals for fungal control is recent. Fusarium verticillioides (synonym=F. moniliforme J. Sheld) sensitivity to the iodine-base agent was determined using two methods. One method used an agar plug taken from an actively growing culture and the other method used isolated conidia. The concentration of the agent required to inhibit growth of F. verticillioides was greater for the culture plug than isolated conidia. Forty-eight strains of F. verticillioides were analyzed representing different states within the United States, countries outside the United States, monocot and dicot plant hosts, and mating types. All strains of F. verticillioides, regardless of geographic origin, plant host, or mating type were sensitive to the test compound. In addition, 25 species of fungi pathogenic to a diverse array of crops were analyzed and demonstrated to be sensitive to this agent. A pesticide developed with an iodine-based a.i. could be an effective control for diverse fungi pathogenic to a range of plant hosts.  相似文献   
22.
Many of the genes encoding important trans porters and metabolic enzymes have been identified over the last ten years. Moreover it has been possible to study the biochemical properties of the corresponding proteins in great detail. It is expected that by 2,010 biochemical functions will have been assigned to many of the products of the approximately 30,000 Arabidopsis genes. We will get closer to understanding the biological function of the gene products by systematic analysis of mutants using knock-out and TILLING approaches. Metabolomics initiatives complement these approaches by providing insight into the changes in cellular ion and metabolite profiles in the mutants, thus giving information essential for the construction of cellular and whole plant models. However, one important dataset especially relevant to multicellular organisms is lacking: the knowledge of the spatial and temporal profiles of ions and metabolite levels at cellular and subcell ular levels. To address this issue, we have developed protein-based nanosensors for several metabolites, providing a set of tools for the determination of cytosolic and subcellular ion (e.g. iron and zinc) and metabolite levels in real time using fluorescence-based microscopy. The prototypes of these sensors were shown to function in vitro and also in vivo, i.e. in yeast and in mammalian cell cultures. One future goal is to expand the set of sensors to a wider spectrum of targets by using the natural spectrum of periplasmic binding proteins from bacteria and by computational design of proteins with altered binding pockets. Application of nanosensor technology to plant cells and tissues will help to elucidate the special and temporal distribution of ions and metabolites.  相似文献   
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ObjectiveTo test the effectiveness and safety of tramadol plus metamizole combined or not with a non-steroidal anti-inflammatory drug (NSAID) for treating moderate to severe chronic neoplastic pain in dogs, and its impact on quality of life (QL).Study designProspective, uncontrolled, open-label, clinical study.AnimalsSixty nine client-owned dogs with multiple forms of cancer and visual analog scale (VAS) pain score ≥40 after receiving NSAIDs for at least 7 days.MethodsThe MN group received metamizole + NSAID, MNT group received metamizole + NSAID + tramadol and MT group received metamizole + tramadol. Pain was scored by the 0 to 100 mm VAS (0 = no pain, 100 = worst pain) and analgesic therapy was considered effective if 25 mm differences in VAS scores were observed between day 0 and the follow ups. The QL was evaluated according to a 0 to 36 scoring method for dogs (0 = worst, 36 = best) and side effects were recorded. Data were registered at day 0 (baseline) and at the first and second follow ups (7 and 14 days after day 0, respectively).ResultsThe MN group had less analgesia at day 7 (25%) and day 14 (42%) than MNT (59%, p = 0.0274; 76%, p = 0.0251, respectively) and MT groups (69%, p = 0.0151; 81%, p = 0.0341, respectively). The QL scores were lower in the MN group at the first (score 23) and second follow up (score 26) than in MNT (27, p = 0.0847; 30, p = 0.0002) and MT (28, p = 0.0384; 31, p = 0.0001) groups. Side effects were more commonly observed in the MN group (87%) than in MNT (24%, p < 0.0001) and MT groups (25%, p = 0.0003) at the first follow up.Conclusions and clinical relevanceTramadol plus metamizole combined or not with NSAID were well tolerated and clinically effective to treat moderate to severe pain in dogs with cancer and improved QL.  相似文献   
25.
Eggplant (Solatium melongena L.) fruits deteriorate rapidly during prolonged storage, mainly due to accelerated senescence of the calyx. Dipping the calyx in a solution containing 200 ppm NAA and 900 ppm prochloraz (active ingredient) retarded calyx senescence and controlled decay. At the end of the growing season in Israel (March-May), dipping the whole fruit in the above solution gave better results than dipping the calyx only. After 14 d of storage at 12°C and 3 d at 20°C, the calyx appeared fresh and green and the fruit was firm. Decay incidence did not exceed 5%. The residual level of prochloraz in fruits that were wholly dipped was less than 0.34 mg 1“'.  相似文献   
26.
ABSTRACT

Due to negative health effects of high sodium intake, it is recommended to reduce the daily salt intake by around 50%. To reduce the sodium content, sodium salts can be exchanged with potassium or magnesium salts. The effect of sodium, potassium, and magnesium chlorides on extractability of proteins from fresh and frozen haddock muscle and minces was studied. Salting with KCl and MgCl2 instead of NaCl changed protein extractability. The highest solubility of the proteins was achieved using Na+. However, at low concentrations, extractability in K+ and Mg2+ is on the same level as Na+, showing that partial substitution of NaCl with KCl or MgCl2 is possible. Freezing affected the structure of tissue and protein properties, resulting in decreased amount of salt soluble proteins.  相似文献   
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Hybrid cauliflowers have been developed to exploit heterosis and to improve uniformity of production. Two breeding systems are commonly employed, self-incompatibility (SI) and cytoplasmic male sterility (CMS). Sibs, assumed to be self-inbred, often contaminate hybrid seed lots in the SI system and whilst self-inbreeding is not possible in the CMS system, plants that look like sibs occur. The objective of this study was to develop microsatellite markers for male and female cauliflower parent lines of both SI and CMS systems and to use them to screen sibs and aberrant plants in F1 hybrids. Fifty six pairs of microsatellite primers were screened and 8 primer pairs produced co-dominant markers in parent plants and two pairs of markers were chosen for purity testing of F1 hybrid seeds. Controlled pollinations were conducted in the glasshouse to produce hybrid and selfed-seeds. These seeds were grown in a field trial to identify morphologically normal and sib plants and to assess the reliability of microsatellite markers in detecting sib plants. Microsatellite analysis of morphological sib plants from the SI system revealed that these were not always self-inbred, in contrast, most self-inbred plants showed normal growth. Similarly, all morphological sibs from the CMS system showed hybrid bands. This suggests that morphological sibs were not always due to selfing but possibly to an interaction between genetic and environmental factors and this requires further investigation.  相似文献   
29.
The global distribution of citrus black spot (CBS) disease, caused by Phyllosticta citricarpa, is climatically constrained, which is evident from its occurrence in citrus growing areas with warm, summer rainfall and its absence from areas with cooler, Mediterranean-type winter rainfall. Various epidemiological and modelling studies have supported this observation, predominantly estimating unsuitability for P. citricarpa in Mediterranean type climates, with no more than marginal suitability estimated at a few localities within some regions with Mediterranean type climates. The study by Martínez-Minaya et al. (European Journal of Plant Pathology, 143, 69–83, 2015), describes an historic sequence of recorded CBS occurrence in parts of South Africa, conducts an autocorrelation analysis and a correlative analysis with Köppen-Geiger climate zones and makes observations about the occurrence of certain Köppen-Geiger climate zones in the European Union. The study suggests that significant portions of the European Union and the broader Mediterranean basin are climatically similar to warm, summer rainfall areas in South Africa where P. citricarpa persists and causes CBS disease and concludes that the potential distribution of P. citricarpa is less constrained by climatic factors than spatial contagion. However, in this critique we expose methodological shortcomings in the Martínez-Minaya et al. (European Journal of Plant Pathology, 143, 69–83, 2015) study and conclude that the study grossly overestimated the extent of the geographical area that could support P. citricarpa, thereby rendering the findings scientifically unreliable.  相似文献   
30.

Purpose

Phytostabilization with native plant species might represent an economically more realistic and cost-effective option than excavation, soil washing, and sludge disposal for rehabilitation of degraded and polluted industrial areas. This work was done to assess the changes induced by native plant revegetation in the chemical properties and mobility-bioavailability of Pb and Zn pollutants of soil and post-washing sludges from an Italian brownfield site of national interest.

Materials and methods

A 5-year native plant revegetation of polluted soil and relative post-washing sludges from a steel plant was achieved in situ and ex situ in pot and in the presence and absence of peat as organic amendment. During the experiment, the vegetation growth was monitored (Adamo et al. In Int J Environ Sci Technol 12(6):1811–1824, 2015). Before and after plant growth, the substrates were studied for pH, organic carbon, and carbonate contents. Lead, Zn, and other metal mobility and leachability were investigated by water extraction. The metal bioavailability was estimated by diethylenetriaminepentaacetic acid (DTPA) extraction at pH 7.3. Sequential extractions (BCR procedure) were used to fractionate Pb and Zn in soil main geochemical forms. Plant ability to uptake metals was evaluated on the three most representative species: Bituminaria bituminosa, Daucus carota, and Dactylis glomerata.

Results and discussion

After 5 years of revegetation with native plants, the substrate pH and organic carbon content were respectively decreased and increased by plant growth, with changes masked by peat treatments. Although metal pollutants in both substrates were characterized by low water solubility and DTPA availability, after plant growth, an increase of rhizospheric Zn, Cu, Fe, and Mn solubility in H2O was detected. According to metal speciation, Pb and Zn were largely occluded in easily reducible manganese/iron oxides and trapped in the mineral structure of silicates, with no visible changes of distribution after plants. Water extraction always underestimated plant uptake, whereas DTPA and sequential extractions better predicted Pb and Zn uptake.

Conclusions

Despite the original extremely low mobility and bioavailability of metal pollutants in both soil and post-washing sludges, the acidification and increase of organic carbon content induced by peat amendments and plant growth enhance the solubility in water of metal-containing compounds. Therefore, attention must be paid to these effects in the long period. A continuous monitoring of the changes of pollutant mobility-bioavailability induced by native plant revegetation of brownfields is crucial to prevent risks to the surrounding environment and human health.
  相似文献   
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