Interspecific germ cell transplantation: a new light in the conservation of valuable Balkan trout genetic resources?     

Jelena Lujić  Zoran Marinović  Simona Sušnik Bajec  Ida Djurdjevič  Béla Urbányi  Ákos Horváth 《Fish physiology and biochemistry》2018,44(6):1487-1498

Interspecific transplantation of germ cells from the brown trout Salmo trutta m. fario and the European grayling Thymallus thymallus into rainbow trout Oncorhynchus mykiss recipients was carried out in order to improve current practices in conservation of genetic resources of endangered salmonid species in the Balkan Peninsula. Current conservation methods mainly include in situ efforts such as the maintenance of purebred individuals in isolated streams and restocking with purebred fingerlings; however, additional ex situ strategies such as surrogate production are needed. Steps required for transplantation such as isolation of high number of viable germ cells and fluorescent labeling of germ cells which are to be transplanted have been optimized. Isolated and labeled brown trout and grayling germ cells were intraperitoneally transplanted into 3 to 5 days post hatch rainbow trout larvae. Survival of the injected larvae was comparable to the controls. Sixty days after transplantation, fluorescently labeled donor cells were detected within the recipient gonads indicating successful incorporation of germ cells (brown trout spermatogonia and oogonia—27%; grayling spermatogonia—28%; grayling oogonia—23%). PCR amplification of donor mtDNA CR fragments within the recipient gonads additionally corroborated the success of incorporation. Overall, the transplantation method demonstrated in this study presents the first step and a possible onset of the application of the germ cell transplantation technology in conservation and revitalization of genetic resources of endangered and endemic species or populations of salmonid fish and thus give rise to new or improved management strategies for such species.  相似文献   

Identification of indigenous fruits with export potential from Mukono district,Uganda: an assessment of two methods     

Riikka Nieminen  Marten Sørensen  Ida Theilade 《Agroforestry Systems》2017,91(5):967-979

Ethnobotanical studies and scorecard-based assessments have been used in identification and prioritisation of indigenous fruit trees with domestication and income potential at local levels. Less has been done to systematically identify species with potential for international markets. This study compared the applicability of the two methods to identify indigenous fruits with economic potential for export. The ethnobotanical study consisted of household and market surveys based on questionnaires, focus group discussions and key-informant interviews. The scorecard was based on pre-determined scoring criteria applied to species identified in the ethnobotanical study. Priority indigenous fruits identified in the ethnobotanical study included Canarium schweinfurtii, Vangueria apiculata, Garcinia buchananii and Tamarindus indica whereas the scorecard assessment identified a different set of species; i.e. V. apiculata, G. buchananii, Myrianthus arboreus, Pseudospondias microcarpa, Phoenix reclinata and Rhus vulgaris as having export potential. The ethnobotanical methods were effective in identifying species with local economic potential and hence high likelihood of adoption by local farmers while the scorecard method was found more useful to identify species with export potential.  相似文献   

Phenotyping of lumbosacral stenosis in Labrador retrievers using computed tomography          下载免费PDF全文

Meenakshi Mukherjee  Jeryl C. Jones  Ida Holásková  Raymond Raylman  Jean Meade 《Veterinary radiology & ultrasound》2017,58(5):565-580

Deep phenotyping tools for characterizing preclinical morphological conditions are important for supporting genetic research studies. Objectives of this retrospective, cross‐sectional, methods comparison study were to describe and compare qualitative and quantitative deep phenotypic characteristics of lumbosacral stenosis in Labrador retrievers using computed tomography (CT). Lumbosacral CT scans and medical records were retrieved from data archives at three veterinary hospitals. Using previously published qualitative CT diagnostic criteria, a board‐certified veterinary radiologist assigned dogs as either lumbosacral stenosis positive or lumbosacral stenosis negative at six vertebral locations. A second observer independently measured vertebral canal area, vertebral fat area, and vertebral body area; and calculated ratios of vertebral canal area/vertebral body area and vertebral fat area/vertebral body area (fat area ratio) at all six locations. Twenty‐five dogs were sampled (lumbosacral stenosis negative, 11 dogs; lumbosacral stenosis positive, 14 dogs). Of the six locations, cranial L6 was the most affected by lumbosacral stenosis (33%). Five of six dogs (83%) with clinical signs of lumbosacral pain were lumbosacral stenosis positive at two or more levels. All four quantitative variables were significantly smaller at the cranial aspects of the L6 and L7 vertebral foramina than at the caudal aspects (P < 0.0001). Fat area ratio was a significant predictor of lumbosacral stenosis positive status at all six locations with cranial L6 having the greatest predictive value (R² = 0.43) and range of predictive probability (25–90%). Findings from the current study supported the use of CT as a deep phenotyping tool for future research studies of lumbosacral stenosis in Labrador retrievers.  相似文献   

The Effect of Cholesterol on the Long-Range Network of Interactions Established among Sea Anemone Sticholysin II Residues at the Water-Membrane Interface     

Sara García-Linares  Ida Alm  Terhi Maula  José G. Gavilanes  Johan Peter Slotte  álvaro Martínez-del-Pozo 《Marine drugs》2015,13(4):1647-1665

Actinoporins are α-pore forming proteins with therapeutic potential, produced by sea anemones. Sticholysin II (StnII) from Stichodactyla helianthus is one of its most extensively characterized members. These proteins remain stably folded in water, but upon interaction with lipid bilayers, they oligomerize to form a pore. This event is triggered by the presence of sphingomyelin (SM), but cholesterol (Chol) facilitates pore formation. Membrane attachment and pore formation require changes involving long-distance rearrangements of residues located at the protein-membrane interface. The influence of Chol on membrane recognition, oligomerization, and/or pore formation is now studied using StnII variants, which are characterized in terms of their ability to interact with model membranes in the presence or absence of Chol. The results obtained frame Chol not only as an important partner for SM for functional membrane recognition but also as a molecule which significantly reduces the structural requirements for the mentioned conformational rearrangements to occur. However, given that the DOPC:SM:Chol vesicles employed display phase coexistence and have domain boundaries, the observed effects could be also due to the presence of these different phases on the membrane. In addition, it is also shown that the Arg51 guanidinium group is strictly required for membrane recognition, independently of the presence of Chol.  相似文献   

Water distribution in brine salted cod (Gadus morhua) and salmon (Salmo salar): a low-field 1H NMR study     

Aursand IG  Gallart-Jornet L  Erikson U  Axelson DE  Rustad T 《Journal of agricultural and food chemistry》2008,56(15):6252-6260

Low-field (LF) (1)H NMR T 2 relaxation measurements were used to study changes in water distribution in lean (Atlantic cod) and fatty (Atlantic salmon) fish during salting in 15% NaCl and 25% NaCl brines. The NMR data were treated by PCA, continuous distribution analysis, and biexponential fitting and compared with physicochemical data. Two main water pools were observed in unsalted fish, T 21, with relaxation times in the range 20-100 ms, and T 22, with relaxation times in the range 100-300 ms. Pronounced changes in T 2 relaxation data were observed during salting, revealing changes in the water properties. Salting in 15% brine lead to a shift toward longer relaxation times, reflecting increased water mobility, whereas, salting in saturated brines had the opposite effect. Water mobility changes were observed earlier in the salting process for cod compared to salmon. Good linear correlations ( F 相似文献   

Hypersensitivity of lettuce leaves inoculated with Bremia lactucae     

F. Virányi  Ida Blok 《European journal of plant pathology / European Foundation for Plant Pathology》1976,82(6):251-254

  相似文献   

Integration of species persistence, costs and conflicts: An evaluation of tree conservation strategies in Cambodia     

Niels Strange  Ida Theilade  So Thea  Arvid Sloth  Finn Helles 《Biological conservation》2007,137(2):223-236

Cambodia forms part of the Indo-Burma hotspot. Its extent of biodiversity, however, is subject to considerable uncertainty, as there has been little systematic collection of flora and fauna. During the Khmer Rouge regime institutions were banned, academics were prosecuted and written documentation systematically destroyed. Compared with neighbouring countries Cambodia has a low population density and relatively large natural areas that are still intact. However, deforestation is expanding rapidly and a significant but un-estimated area of forest has been degraded by development of agro-industries, encroachment, illegal logging, over-harvesting and forest fire as well as the use of chemicals during war. The purpose of the paper is to: (i) apply reserve selection methods to design more robust conservation networks when knowledge of species occurrence is incomplete and habitat is threatened, and (ii) evaluate the usefulness of systematic conservation planning in a developing country where data are limited and institutions for implementation are weak. This study investigates the performance of four non-probabilistic strategies: (i) a so-called ‘rule of thumb’, (ii) hotspot, (iii) minimum cost representation, and (iv) maximum coverage; and one probabilistic design strategy, i.e. maximum expected coverage. The maximum expected coverage approach is between 15% and 24% more efficient than the non-probabilistic strategies. Finally, the relevance of such tools to real-world conservation planning in Cambodia is investigated. By incorporating experts in the generation of data, running the models and setting up premises, they acknowledge that it is possible to contribute to more systematic conservation planning in developing countries.  相似文献   

Appropriate secondary absolute erythrocytosis in a horse     

Belli CB  Baccarin RY  Ida KK  Fernandes WR 《The Veterinary record》2011,169(23):609

  相似文献   

In support of academic freedom     

Executive Committee of the International Human Rights Network of Academies  Scholarly Societies  Aluwihare A  Cohen-Tannoudji C  Daar AS  Jacob F  Koiller B  Nicolaisen I  Polanyi J  Selih A  van Dijk P  Vesentini E  Wiesel T  Corillon C 《Science (New York, N.Y.)》2007,316(5833):1840

  相似文献   

Dissolved organic carbon (doc) and dissolved organic nitrogen (don) content of an arenosol as affected by liming in a pot experiment     

Tibor Filep  Ida Kincses  Péter Nagy 《Archives of Agronomy and Soil Science》2013,59(1):111-117

The effect of liming materials was investigated on the dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) content of the soil in a pot experiment on an acidic soil using oat (Avena sativa L. ) as an indicator plant. Soil samples were taken three times during the growing season. The lime rates applied were 0, 1, 2, 3 g calcite/pot and 0.92, 1.84, 2.76 g dolomite/pot, respectively. Due to an increase in soil pH and microbial activity the DOC concentration significantly increased with increasing lime doses at all three samplings. An exponential relationship was found between soil pH and DOC concentration: y v = v 0.3733e 0.7893x , r v = v 0.903***. Lime had a significant effect on DON concentration at the first sampling, while for the remainder of the growing period no further significant increases were found. This could be explained by the fact that the biodegradability of DOC and DON differs under conditions of the experiment, resulting in a decrease in the N content of the dissolved organic matter, while the amount of DOM and DOC increased with increasing pH. Because of the above mentioned facts the DOC/DON ratio increased significantly with liming. There were no significant changes in the dissolved organic carbon content of the soil during the 15-week growing period, while DON concentration decreased significantly. It can be explained by the initial addition of N fertilizer, which increased the DON quantity at the first sampling in the soil.  相似文献