Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a     

D'aes J  Hua GK  De Maeyer K  Pannecoucque J  Forrez I  Ongena M  Dietrich LE  Thomashow LS  Mavrodi DV  Höfte M 《Phytopathology》2011,101(8):996-1004

Pseudomonas CMR12a was previously selected as an efficient biocontrol strain producing phenazines and cyclic lipopeptides (CLPs). In this study, biocontrol capacity of Pseudomonas CMR12a against Rhizoctonia root rot of bean and the involvement of phenazines and CLPs in this ability were tested. Two different anastomosis groups (AGs) of Rhizoctonia solani, the intermediately aggressive AG 2-2 and the highly aggressive AG 4 HGI, were included in growth-chamber experiments with bean plants. The wild-type strain CMR12a dramatically reduced disease severity caused by both R. solani AGs. A CLP-deficient and a phenazine-deficient mutant of CMR12a still protected bean plants, albeit to a lesser extent compared with the wild type. Two mutants deficient in both phenazine and CLP production completely lost their biocontrol activity. Disease-suppressive capacity of CMR12a decreased after washing bacteria before application to soil and thereby removing metabolites produced during growth on plate. In addition, microscopic observations revealed pronounced branching of hyphal tips of both R. solani AGs in the presence of CMR12a. More branched and denser mycelium was also observed for the phenazine-deficient mutant; however, neither the CLP-deficient mutant nor the mutants deficient in both CLPs and phenazines influenced hyphal growth. Together, results demonstrate the involvement of phenazines and CLPs during Pseudomonas CMR12a-mediated biocontrol of Rhizoctonia root rot of bean.  相似文献   

Resistenzigenschaften im Gersten- und Weizensortiment Gatersleben 6. Prüfung von Gersten auf ihr Verhalten gegen Gelbrost(Puccinia striiformis West. syn.P. glumarum (Schm.) Erikss. et Henn.)     

Ilse Nover  Christian O. Lehmann 《Genetic Resources and Crop Evolution》1966,14(1):257-262

Zusammenfassung Von 380 Winter- und 1400 Sommergersten des Sortiments Gatersleben wurde in Halle das Verhalten gegen die virulente Rasse 24 vonPuccinia striiformis West, untersucht. Als hochresistent erwiesen sich 5 Winter- und 43 Sommerformen. Ein großer Teil dieser Sommergersten, darunter auch wertvolle Kombinationen von Gelbrost- mit Zwergrost- bzw. Mehltauresistenz, stammen aus Aethiopien. Resistenz zeigten weitere 88 Proben oder ca. 5% des Sortiments. In 12% lagen Gemische anfälliger mit resistenten Pflanzen vor. 80% der Gersten waren mäßig bis stark anfällig für Gelbrost.

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Summary Stripe rust reaction of 380 winter and 1400 spring barley varieties of the Gatersleben collection was tested in the greenhouse as well as in the disease nursery by artificial infection with the virulent race 24 ofPuccinia striiformis West. Five winter and 43 spring barley varieties have proved to be highly resistant. A large number of the latter were collected in Ethiopia, among them are useful combinations of stripe rust with dwarf rust resistance and mildew resistance, respectively. Resistance was shown by other 88 varieties or by about 5% of the total. Susceptible as well as resistant plants were found in 12% of the samples. 80% of the varieties were moderately or highly susceptible to stripe rust.

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am ¶rt;au 380 1400 24 . 5 43 . ¶rt; , , . 88 5% . 12% . 80% .

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Veränderungen der Muskelfasertypenverteilung im M. longissimus dorsi von Ebern während des Wachstums, dargestellt mit verschiedenen histochemischen Methoden     

J. Wegner    Ilse  Fiedler  Danuta  Klosowska  B. Klosowski  Brigitte  Ziegan 《Anatomia, histologia, embryologia》1993,22(4):355-359

Changes in the distribution of muscle-fiber-types from the M. longissimus dorsi of male pigs. A Histochemical Approach Samples of the M. longissimus dorsi from growing up pigs were taken by a shooting-biopsy. The types of muscle-fibers could be demonstrated by two different histochemical staining procedures. With these two histochemical staining procedures significant differences between the different structure of the muscle-fibers during increasing age of the pigs could be detected.  相似文献   

Resistenzeigenschaften im Gersten- und Weizensortiment Gatersleben     

Ilse Nover  Rudolf Mansfeld 《Genetic Resources and Crop Evolution》1959,7(1):29-36

Ohne Zusammenfassung  相似文献   

Influence of outlying values and variations between sampling days on reference ranges for clinical chemistry in budgerigars (Melopsittacus undulatus)     

Scope A  Schwendenwein I  Frommlet F 《The Veterinary record》2005,156(10):310-314

Reference ranges for clinical chemistry variables were established for 99 budgerigars sampled monthly for 12 months. The data from the repeated measurements were compiled to obtain the most reliable population-based reference range defined to be relevant for the population of budgerigars over the whole year. Outlying values were excluded by reference to the population-based reference range and to previously determined values from the same individual, following the guidelines of the International Federation of Clinical Chemistry. The exclusion of outliers significantly reduced the calculated reference ranges.  相似文献   

Differential expression of non-cytoplasmic Actinobacillus pleuropneumoniae proteins induced by addition of bronchoalveolar lavage fluid     

Jacobsen ID  Meens J  Baltes N  Gerlach GF 《Veterinary microbiology》2005,109(3-4):245-256

Actinobacillus (A.) pleuropneumoniae is the causative agent of a porcine pleuropneumonia occurring worldwide. In order to identify novel non-cytoplasmic putative virulence-associated proteins, we prepared fractions enriched in surface-associated proteins for differential proteome analysis by two-dimensional (2D) gel electrophoresis and quadrupole time-of-flight mass spectrometry (Q-Tof MS). Bacteria grown under standard culture conditions were compared to an ex vivo model based on the addition of bronchoalveolar lavage fluid (BALF) to the culture media. Twelve proteins were found to be upregulated upon induction with BALF, among them a superoxide dismutase, a parvulin-like peptidy-prolyl isomerase, a polynucleotide phosphorylase and the highly immunogenic lipoprotein OmlA. Four of the proteins upregulated by BALF were additionally constitutively expressed by an isogenic A. pleuropneumoniae fur deletion mutant and could be identified by Q-Tof MS as the heat shock protein GroES, a putative dipeptide transporter, a putative metal ion transporter and a conserved protein of unknown function. In silico analysis of the putative promoter regions of the encoding genes revealed putative Fur boxes upstream of two genes, one of which encodes part of a putative metal ion transporter. An isogenic mutant with a deletion in this protein was constructed and designated as A. pleuropneumoniae Deltafui. Analysis of the mutant in an aerosol infection model revealed symptoms indistinguishable from those seen upon infection with wild type A. pleuropneumoniae. This result implies that not all proteins upregulated by BALF are directly involved in A. pleuropneumoniae virulence.  相似文献   

Anbau von Echter Jute in den Amazonasgebieten     

Dr. Ilse Rasch 《Plant foods for human nutrition (Dordrecht, Netherlands)》1954,1(4):359-362

Ohne Zusammenfassung  相似文献   

Hypothyroid goitre in a ram: chemical analysis gives indirect evidence for a structurally altered type of ovine thyroglobulin     

Sipos W  Miller I  Fountoulakis M  Schmoll F  Patzl M  Schwendenwein I  Rapp E  Taxacher A  Gemeiner M 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2004,51(2):90-96

The thyroglobulin of a ram of the East Friesian milk sheep breed suffering from goitre was investigated by physico- and immunochemical methods. The respective ram was the only animal amongst the other sheep of the flock, that exhibited severe goitre, additionally showing depressed behaviour. Results of the thyroid-stimulating hormone response test were indicative of hypothyroidism. The dysfunction of the thyroid gland could be treated by additional iodine supplementation quite successfully, although all sheep had been given iodinated cattle salt throughout the course of the history. Without reducing conditions sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of isolated thyroglobulin molecules of the ram and control sheep did not reveal different band patterns, but under reducing conditions different band patterns were evident for the respective animals: the ram's thyroglobulin displayed two main bands, those of healthy reference sheep only one. Both bands reacted equally with anti-thyroglobulin antibodies, even with those produced by immunizing rabbits with single bands. The reduced single thyroglobulin band of healthy sheep corresponded to a truncated form of that molecule, whereas the additional main band of the ram was a more resistant, intact thyroglobulin subunit, as was shown by mass spectrometry. In conclusion, results of physico- and immunochemical investigations gave evidence of a modification of thyroglobulin with suspected different iodine binding properties in the ram. The latter finding may have clinical relevance in similar cases in other species, as it is an example of the impact that a minor change in a protein molecule may have on a complete metabolic pathway. Additionally, it could be shown, that in the ovine species the generally found single main band of thyroglobulin after reduction is a truncated form and not an intact subunit. This truncation seems to be induced in vitro by the reductive sample pretreatment prior to SDS-PAGE.  相似文献   

Biological variation, individuality and critical differences of eight biochemical blood constituents in budgerigars (Melopsittacus undulatus)     

Scope A  Schwendenwein I  Frommlet F 《The Veterinary record》2006,159(25):839-843

The aim of the study was to obtain basic data on individual biological variation, the required number of specimens to define the homeostatic setpoint (the aspired value of a variable adjusted to the individuals homeostasis) and critical differences of selected chemistry parameters in budgerigars (Melopsittacus undulatus). Blood from 99 healthy budgerigars was sampled 12 times at four-week intervals. Aspartate aminotransferase (ast), calcium, creatine kinase (ck), glutamate dehydrogenase (gldh), glucose, lactate dehydrogenase (ldh), total protein and uric acid were investigated. The indices of individuality obtained in the present study were relatively low (total protein 0.93, ast 1.02, gldh 1.04, ck 1.12, ldh 1.24, uric acid 1.26, glucose 1.39, calcium 1.61) and suggest that population-based reference limits might be useful. Comparison of data showed that the application of intraindividual reference values identified much greater variation in the reference values than using conventional population-based reference limits. Otherwise, the moderate to low degrees of individuality may allow the use of reference values of one individual as a standard for another individual of the same species. The critical differences that define the change needed between two serial results to indicate a biological change was highest in gldh with 120 per cent or 1.7 U/l. In calcium, a change of 30.5 per cent or 0.5 mmol/l would be significant. In the other parameters critical differences varied between 38 per cent in glucose, up to 93 per cent in uric acid.  相似文献   

Treatment of nephroblastoma with polycythaemia by nephrectomy in a rabbit     

Hassan J  Katic N  Klang A  Schwendenwein I  Böhler A  Künzel F 《The Veterinary record》2012,170(18):465

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