Immunohistochemistry Diagnosis of an Ovarian Dysgerminoma in One Bitch     

JK Park  MJ Goo  IH Hong  MR Ki  JY Han  KS Jeong 《Reproduction in domestic animals》2009,44(5):855-858

An ovarian enlargement (diameter, 8 cm) was identified and surgically excised from a 5-year-old female dog. Microscopic examination of the multinodular neoplasm revealed sheets of polygonal neoplastic cells with large nuclei, frequent mitosis, necrosis and haemorrhage. Immunohistochemically, the neoplastic cells were positive for vimentin and alkaline phosphatase but were negative for CD3, CD79a, cytokeratin, alpha-fetoprotein, inhibin-α and S-100. The histopathological diagnosis of the mass was unilateral ovarian dysgerminoma.  相似文献   

Interactions between CXCR4 and CXCL12 promote cell migration and invasion of canine hemangiosarcoma          下载免费PDF全文

K. S. Im  A. J. Graef  M. Breen  K. Lindblad‐Toh  J. F. Modiano  J.‐H. Kim 《Veterinary and comparative oncology》2017,15(2):315-327

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Specific labeling of neurogenic, endothelial, and myogenic differentiated cells derived from human amniotic fluid stem cells with silica-coated magnetic nanoparticles     

Lee JK  Chun SY  Im JY  Jin HK  Kwon TG  Bae JS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(8):969-975

Stem cell based cell therapies offer significant potential for the field of regenerative medicine. Human amniotic fluid stem cells (hAFSCs) are an attractive source for lineage-specific differentiated stem cell therapy since they have properties that are able to differentiate into cells representing all three germ layers. To better understand the fate and location of implanted hAFSCs, a means to monitor cells in living subjects is essential. Here, we showed that differentiated cells, such as neurogenic, endothelial, and myogenic cells, derived from hAFSCs can be effectively labeled by the FITC-incorporated silica-coated nanoparticles, MNPs@SiO2 (FITC), although the labeling efficacy and cytotoxicity were distinct depending on the differentiated cell type. In addition, we observed that MNPs@SiO2-labeled cells provided sufficient signals for detection by optical and confocal microscope imaging when transplanted into the mice. These results suggest that the fluorescent dye incorporated MNPs@SiO2 are a useful tool for the cell labeling and in vivo tracking of differentiated cells derived from hAFSCs.  相似文献   

Sequential bacteriological observations in relation to cell-mediated and humoral antibody responses of cattle infected with Mycobacterium paratuberculosis and maintained on normal or high iron intake   总被引：3，自引：0，他引：3  

AWD LEPPER  CR WILKS†  M. KOTIW†  JT WHITEHEAD†  KS SWART† 《Australian veterinary journal》1989,66(2):50-55

Twenty calves were orally infected with Mycobacterium paratuberculosis before weaning. Ten of these plus 4 non-infected controls were maintained on elevated dietary iron intake from 6 to 33 months of age. During this time, in which the majority of animals were bred, the influence of increased dietary iron upon tests of cellular and humoral immune responsiveness to antigens of the organism were monitored. Results were examined in relation to the organism's capacity to multiply and infect up to 7 portions of the intestinal tract. No significant differences were detected in the degree of intestinal disease or pattern of faecal excretion of M. paratuberculosis in iron supplemented and non-supplemented cattle. Cutaneous delayed-type hypersensitivity (DTH) to johnin PPD developed at 1 month and in-vitro lymphocyte and immunostimulatory activity (LS) to this antigen at 2 months after infection. LS indices were significantly reduced in magnitude in iron-supplemented cattle (p less than 0.01). Most ELISA antibody responses were positive 10 to 17 months after infection and preceded the fewer number of CF responses by several months. Neither of the antibody tests was affected by elevated iron intake. Generally, complete or partial resistance to paratuberculosis was associated with sustained positive monthly LS tests (index greater than or equal to 2.0), whereas antibody levels tended to be sustained only in the more severely affected cattle. Although neither test system was affected by pregnancy the ELISA failed to detect a significant proportion of cattle chronically shedding M. paratuberculosis in faeces.  相似文献   

Resistance to synthetic pyrethroids in South Australian populations of sheep lice (Bovicola ovis)     

PJ JAMES  PE SAUNDERS  KS COCKRUM  KJ MUNRO 《Australian veterinary journal》1993,70(3):105-108

SUMMARY Lice were collected from 71 flocks detected as Infested at market Inspection in 1990 and 1991, from 16 flocks where resistance was suspected and from 31 flocks from Kangaroo Island. Susceptibility to cypermethrin was measured by a treated surface technique and survival of lice at 5 ppm or greater was taken as an indication of resistance. The prevalence of resistance was 34% in louse populations from the market inspection sample, 50% in flocks in which resistance was suspected and 68% in flocks from Kangaroo Island. Most resistance factors were in the range 1 to 20 although one louse population with a resistance factor of 91 was found on Kangaroo Island.  相似文献   

Change in weed control studies of rice paddy fields in Korea   总被引：1，自引：0，他引：1  

Soon Chul Kim  Il bin Im 《Weed Biology and Management》2002,2(2):65-72

This paper was reviewed on the research status of weed control according to cultivating patterns of rice in Korea. Weed control study in a nursery bed was chiefly carried out to control the species of barnyardgrass at the times of machine transplanting. Propanil and nitrofen were applied successfully in wet-nursery beds, and chlornitrofen and pyrazolate/butachlor were also successfully applied in protected semi-irrigated rice nursery beds in the 1970s. Sequential application of herbicides in the mid-1980s has resulted in the end of hand weeding. In machine transplanting, basic research such as the selection of herbicides was conducted in the early 1970s, and its related research including crop injury, seedling age, and reaction of cultivars were done in the late 1970s to early 1980s. Effects of the continuous application of herbicides and its methods of labor saving for rice cultivation were studied in the mid to late 1980s. When the rice planting methods were newly established, such as transplanting when seedlings were 10-days-old and direct seeding from the early 1990s, weed control research relating to herbicides was carried out (e.g. herbicide registration, crop injury, water management, appropriate application time, and neighboring application with insecticide). Weed control for the dill seeding of fields in wetted and reclaimed saline land have been experimented with, particularly in terms of the physio-ecological characteristics and the control of problematic weeds, and the resistance of weeds to herbicides was also investigated systematically.  相似文献   

Letter to the Editor          下载免费PDF全文

L.C. Visser DVM  MS  DACVIM  M.K. Im DVM  L.R. Johnson DVM  MS  PhD  DACVIM  J.A. Stern DVM  PhD  DACVIM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(4):926-926

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Effect of synthetic agonists of toll-like receptor 9 on canine lymphocyte proliferation and cytokine production in vitro     

Im Hof M  Williamson L  Summerfield A  Balmer V  Dutoit V  Kandimalla ER  Yu D  Zurbriggen A  Doherr MG  Peel J  Roosje PJ 《Veterinary immunology and immunopathology》2008,124(1-2):120-131

Synthetic agonists of TLR9 containing novel DNA structures and R'pG (wherein R=1-(2'-deoxy-beta-d-ribofuranosyl)-2-oxo-7-deaza-8-methyl-purine) motifs, referred to as immune modulatory oligonucleotides (IMOs), have been shown to stimulate T(H)-1-type-immune responses and potently reverse allergen-induced T(H)-2 responses to T(H)-1 responses in vitro and in vivo in mice. In order to investigate the immunomodulatory potential of IMOs in dogs, canine peripheral blood mononuclear cells (PBMC) from healthy dogs were stimulated with three different IMOs and a control IMO, alone or in combination with concanavalin A (ConA). Lipopolysaccharide (LPS) was used as a positive control for B lymphocyte activation. Carboxyfluorescein diacetate succinimidyl ester and phenotype staining was used to tag proliferating T and B lymphocytes (CD5(+) and CD21(+)) by flow cytometry. Real-time PCR and ELISA were processed to assay cytokine production of IFN-gamma, IL-10, TGF-beta, IL-6 and IL-10. Like LPS, IMOs alone induced neither proliferation of CD5(+) T cells nor CD21(+) B cells, but both LPS and IMO had the capacity to co-stimulate ConA and induced proliferation of B cells. In combination with ConA, one of the IMOs (IMO1) also induced proliferation of T cells. IMO1 also significantly enhanced the expression of IFN-gamma on the mRNA and protein level in canine PBMC, whereas expression of IL-10, TGF-beta and IL-4 mRNAs was not induced by any of the IMOs. These results indicate that in canine PBMC from healthy dogs, IMO1 was able to induce a T(H)-1 immune response including T- and B-cell proliferation.  相似文献   

Freshwater turtle admissions to a wildlife hospital in South-East Queensland,Australia over an eleven-year period, 2010–2021     

KS O'Leary  A Hill  RJT Doneley 《Australian veterinary journal》2023,101(6):258-264

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Histopathological retrospective study of canine renal disease in Korea, 2003~2008     

Ji-Young Yhee  Chi-Ho Yu  Jong-Hyuk Kim  Keum-Soon Im  Seung-Ki Chon  Jung-Hyang Sur 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(4):277-283

Renal disease includes conditions affecting the glomeruli, tubules, interstitium, pelvis, and vasculature. Diseases of the kidney include glomerular diseases, diseases of the tubules and interstitium, diseases of renal pelvis, and developmental abnormalities. Renal tissue samples (n = 70) submitted to the Department of Veterinary Pathology of Konkuk University from 2003 to 2008 were included in this study. Tissue histopathology was performed using light microscopy with hematoxylin and eosin stains. Masson''s trichrome, Congo Red, and Warthin starry silver staining were applied in several individual cases. Glomerular diseases (22.9%), tubulointerstitial diseases (8.6%), neoplastic diseases (8.6%), conditions secondary to urinary obstruction (24.3%), and other diseases (35.7%) were identified. Glomerulonephritis (GN) cases were classified as acute proliferative GN (5.7%), membranous GN (4.3%), membranoproliferative GN (4.3%), focal segmental GN (2.9%), and other GN (4.2%). The proportion of canine GN cases presently identified was not as high as the proportions identified in human studies. Conversely, urinary obstruction and end-stage renal disease cases were relatively higher in dogs than in human populations.  相似文献