Genetic manipulation for enhancing calcium content in potato tuber     

Park S  Kang TS  Kim CK  Han JS  Kim S  Smith RH  Pike LM  Hirschi KD 《Journal of agricultural and food chemistry》2005,53(14):5598-5603

Increased calcium (Ca) in potatoes may increase the production rate by enhancing tuber quality and storability. Additionally, increased Ca levels in important agricultural crops may help ameliorate the incidence of osteoporosis. However, the capacity to alter Ca levels in potato tubers through genetic manipulations has not been previously addressed. Here we demonstrate that potato tubers expressing the Arabidopsis H+/Ca2+ transporter sCAX1 (N-terminal autoinhibitory domain truncated version of CAtion eXchanger 1) contain up to 3-fold more Ca than wild-type tubers. The increased Ca appears to be distributed throughout the tuber. The sCAX1-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through successive generations. The expression of sCAX1 does not appear to alter potato growth and development. Furthermore, increased Ca levels in sCAX1-expressing tubers do not appear to alter tuber morphology or yield. Given the preponderance of potato consumption worldwide, these transgenic plants may be a means of marginally increasing Ca intake levels in the population. To our knowledge, this study represents the first attempts to use biotechnology to increase the Ca content of potatoes.  相似文献   

SNP Markers Discovery in Soybean with Use of Degenerate Oligolucleotide Primed PCR     

There are several strategies that can be applied in SNP discovery  as for example the locus-specific amplification of target genome regions 《分子植物育种》2007,5(2):248-249

There are several strategies that can be applied in SNP discovery, as for example the locus-specific amplification of target genome regions （Primmer et al., 2002; Van et al., 2004） or simultaneous assembly of anonymous sequences which are the product of whole genome shotgun sequencing （Webber and Myers, 1997） or reduced representation shotgun sequencing （Altshuler et al., 2000）. The majority of these methods are highly cost-prohibitive, however, and there is a necessity for development of new strategies that would be more suitable for small and medium-scale laboratories.  相似文献   

DNA profiling and genetic diversity of Korean soybean (<Emphasis Type="Italic">Glycine max</Emphasis> (L.) Merrill) landraces by SSR markers     

Mun Sup Yoon  Jeongran Lee  Chang Yung Kim  Jung Hoon Kang  Eun Gi Cho  Hyung Jin Baek 《Euphytica》2009,165(1):69-77

Approximately 7,000 accessions of Korean soybean (Glycine max (L.) Merrill) landraces, largely composed of three collections, the Korea Atomic Energy Research Institute’s soybean (KAS), the Korean Crop Experiment Station’s soybean (KLS) and the Korean Agricultural Development and Technology Center’s soybean (KADTC) collections, have been conserved at the Rural Development Administration (RDA) genebank in Korea. The accessions within collections were classified based on their traditional uses such as sauce soybean (SA), sprouted soybean (SP), soybean for cooking with rice (SCR), and OTHERS. A total of 2,758 accessions of Korean soybean landraces were used to profile and to evaluate genetic structure using six SSR loci. A total of 110 alleles were revealed by at the six SSR loci. The number of alleles per SSR locus ranged from 9 to 39 in Satt187 and Satt_074, respectively. The number of alleles ranged from 87 in the KADTC collection to 96 in the KLS collection, and from 63 in the SCR group to 95 in the SP group. Nei’s average genetic diversity ranged from 0.68 to 0.70 across three collections, and 0.64 to 0.69 across the usage groups. The average between-group differentiation (G _st) was 0.9 among collections, and 4.1 among the usage groups. The similar average diversity among three collections implies that the genetic background of the three collections was quite similar or that there were a large number of duplicate accessions in three collections. The selection from the four groups classified based upon usage may be a useful way to select accessions for developing a Korean soybean landrace core collection at the RDA genebank. DNA profile information of accessions will provide indications of redundancies or omissions and aid in managing the soybean collection held at the RDA genebank. The information on diversity analysis could help to enlarge the genetic diversity of materials in breeding programs and could be used to develop a core collection.  相似文献   

Determination of chlorate and chlorite and mutagenicity of seafood treated with aqueous chlorine dioxide.   总被引：3，自引：0，他引：3  

J Kim  M R Marshall  W X Du  W S Otwell  C I Wei 《Journal of agricultural and food chemistry》1999,47(9):3586-3591

The use of chlorine dioxide (ClO(2)) as a potential substitute for aqueous chlorine to improve the quality of seafood products has not been approved by regulatory agencies due to health concerns related to the production of chlorite (ClO(2)(-)) and chlorate (ClO(3)(-)) as well as possible mutagenic/carcinogenic reaction products. Cubes of Atlantic salmon (Salmo salar) and red grouper (Epinephelus morio) were treated with 20 or 200 ppm aqueous chlorine or ClO(2) solutions for 5 min, and extracts of the treated fish cubes and test solutions were checked for mutagenicity using the Ames Salmonella/microsome assay. No mutagenic activity was detected in the treated fish samples or test solutions with ClO(2). Only the sample treated with 200 ppm chlorine showed weak mutagenic activity toward S. typhimurium TA 100. No chlorite residue was detected in sea scallops, mahi-mahi, or shrimp treated with ClO(2) at 3.9-34.9 ppm. However, low levels of chlorate residues were detected in some of the treated samples. In most cases, the increase in chlorate in treated seafood was time- and dose-related.  相似文献   

Loss of pendimethalin in runoff and leaching from turfgrass land under simulated rainfall   总被引：2，自引：0，他引：2  

Lee YD  Kim HJ  Chung JB  Jeong BR 《Journal of agricultural and food chemistry》2000,48(11):5376-5382

A field study was undertaken to investigate runoff and leaching loss of the herbicide pendimethalin in turfgrass land of loamy sand soil. A series of plots constructed in a golf course fairway were surface-applied with pendimethalin SC formulation at the rate of 2. 25 or 4.50 kg a.i./ha and subjected to simulated rainfall at 2.0 cm/day for 10 consecutive days. Runoff losses of pendimethalin were the highest at the first rainfall and then gradually decreased with time. The first runoff event contained pendimethalin in its highest concentration, and in subsequent runoff samples the concentration decreased exponentially. The ranges of pendimethalin concentration were 80.9-18.2 and 177.4-48.6 microgram/L in the standard and double doses, respectively. Total losses by 20 cm of rainfall for 10 days reached 0.81 and 1.22% of the initial deposits at 2.25 and 4.50 kg a. i./ha, respectively. Pendimethalin concentration in the leachate collected at 30-cm soil depth was quite lower than that in the runoff, and the concentration rapidly decreased from 4.3-4.7 to 0. 2-0.4 microgram/L during the 10 days of rainfall treatment. Soil residue analysis at 45 and 90 days after pendimethalin treatment showed that more than 90% of the residue remained at the top 10 cm of soil depth. Low runoff and leaching confirmed that lateral and downward movement of the herbicide should be limited in turf soil. The half-life of pendimethalin under field conditions was 23-30 days and was not affected by application dose and rainfall treatment, but longer persistence was observed under laboratory conditions. Considering low runoff and leaching, as well as relatively short persistence in soil, it is concluded that little environmental carryover of pendimethalin would be expected in turfgrass land.  相似文献   

Estimation of Dust Emission Amount for a Dust Storm Event Occurred in April 1998 in China     

In  Hee-Jin  Park  Soon-Ung 《Water, air, and soil pollution》2003,148(1-4):201-221

The threshold friction velocities for dust rises in inland China are examined using WMO synoptic station data and the soil types in the source region to estimate dust emission amounts for a dust event occurred from 13 to 24 April 1998 in China. The dust-rise reports in the WMO synoptic data are used for the delineation of dust source regions that are found to be composed of the Gobi, Sand and Loess regions according to soil types in inland China. The estimated threshold friction velocities with the use of wind speeds observed in the Gobi, Sand and Loess regions are found to be about 60, 50, and 40 cm s^-1, respectively. The estimated dust-rise occurrencegrids by both the grid interpolated observed wind and the MM5 model wind well coincide with the areas of strong wind speeds. However, the dust-rise areas estimated by the model are larger than those estimated by the observed wind, whereas the friction velocities estimated by the model in the dust-rise areas are much smaller than those estimated by the observed wind, which result in less amount of dust emission by the model in all source regions. The 10-day accumulated total dust emission estimated by the observed wind during the dust storm event is found to be 4.64 × 10⁸ ton in the whole source region of 3.29 × 10⁶ km². Of which 85, 9 and 6% are, respectively, contributed by the Gobi, Sand and Loess regions,suggesting the dominant emission from the Gobi region for this event.The model estimated total dust emission is found to be about 56% of that estimated by the observed wind, indicating some improvement of the model being required.  相似文献   

玉米精播机漏播补偿系统设计   总被引：21，自引：2，他引：21  

金衡模  高焕文 《农业机械学报》2002,33(5):44-47

目前玉米免耕播种机的播种质量仍低于常规播种。本文讨论了微机控制的排种监测及漏播补偿装置，通过机电结合手段解决漏播问题。台架试验表明本装置能够准确发现漏播，并通过采取的措施实现及时补种，补种成功率可达90％以上。  相似文献   

Effects of coffee bean aroma on the rat brain stressed by sleep deprivation: a selected transcript- and 2D gel-based proteome analysis     

Seo HS  Hirano M  Shibato J  Rakwal R  Hwang IK  Masuo Y 《Journal of agricultural and food chemistry》2008,56(12):4665-4673

  相似文献   

Nomenclature and genetic relationships of apples and pears from Terceira Island     

Nahla Bassil  Kim E. Hummer  Joseph D. Postman  Gennaro Fazio  Angela Baldo  Isabel Armas  Roger Williams 《Genetic Resources and Crop Evolution》2009,56(3):339-352

Heritage apple (Malus domestica Borkh. hybrids) and pear (Pyrus communis L. hybrid) trees grow in villages throughout Terceira Island, Azores, Portugal. Some of these pears have different names but similar morphology. The objective of this study was to determine synonymy, homology, and phylogeny of apples and pears from Terceira and to examine potential relationships of the island pears with standard apples and pears of Portuguese or American descent. Nine apple microsatellite markers were used to determine genetic relationships. Distance- and parsimony-based cluster analysis grouped these genotypes into separate apple and pear clades. The Terceira apples were divided into two clades: the maçā and the reineta-reinette. Among the 17 heritage apple genotypes, seven unique accessions were identified and four groups of synonyms, or possibly clones, were detected including: ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares; ‘Reineta Castanha’ and ‘Reineta Verde Miuda’; ‘Maçā Pêra,’ ‘Maçā Calhau’, ‘Pêro Branco’ from Salga and from Terra-Chā and ‘Maçā Marmelo’; and the five genotypes ‘Maçā Sao Joao’, ‘Malápio Rosa’, ‘Maçā Gaspar’, ‘Maçā Branca’ and ‘Maçā Pato’. In addition, two homonyms were detected. ‘Pêro Vermelho’ from Terra Chā was a separate genotype from a tree from Doze Ribeiras of the same name, but Pêro Branco from Terra Chā appears to be a clone that can be distinguished by an additional allele at CH1F07a from a tree with that name from Salga. One pair of apple clones, ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares appear to be derived from an unreduced gamete of ‘Golden Delicious.’ Another apple genotype ‘Maçā Acida’ could be a sibling of the ‘Maçā Pêra’ clonal group. Other tested standard apples from the US genebank were unrelated to Terceira genotypes. Of the seven heritage pears, five unique genotypes and one pair of synonyms were detected. ‘Pêra Papo Pintassilgo’ from Raminho and ‘Pêra Vermelha’ from the nursery of Serviço de Desenvolvimento Agario da Terceira (SDAT) were synonyms. ‘Passans du Portugal’ was related to ‘Pêra Cabaca’ but other standard pears from the US genebank were unrelated to Terceira genotypes. Future studies will include additional apple and pear cultivars from other Islands of the Azores and continental Portugal, and wild Asian species to further explore genetic relationships.  相似文献   

Chemical Composition Change in TSP Due to Dust Storm at Gosan, Korea: Do the Concentrations of Anthropogenic Species Increase Due to Dust Storm?     

Na Kyung Kim  Hye-Jin Park  Yong Pyo Kim 《Water, air, and soil pollution》2009,204(1-4):165-175

Aerosol composition change between dust storm and non-dust storm periods in spring is studied using the total suspended particle data measured at Gosan, a background area in Korea. The concentrations of eight inorganic ions and 12 elements were analyzed for the TSP samples collected from March 1992 to May 2006. Two-step statistical analyses were carried out for the data: F test and t test. The concentrations of anthropogenic ionic species showed increasing trend since 2003. The absolute concentrations of most anthropogenic species such as sulfate or nitrate increased (from 7.33 to 9.25 µg m^?3 and from 2.04 to 4.84 µg m^?3, respectively) during the dust storm period. However, the enrichment factors or normalized concentrations based on Al of most anthropogenic species decreased during dust storm period (factor of 0.1–0.5). It suggests that, in general, relative importance of anthropogenic species during dust storm would be not high.  相似文献