Contribution of soil bacteria isolated from different regions into crude oil and oil product degradation     

Puntus  Irina F.  Borzova  Oksana V.  Funtikova  Tatyana V.  Suzina  Nataliya E.  Egozarian  Nataliya S.  Polyvtseva  Valentina N.  Shumkova  Ekaterina S.  Akhmetov  Lenar I.  Golovleva  Ludmila A.  Solyanikova  Inna P. 《Journal of Soils and Sediments》2019,19(8):3166-3177

Journal of Soils and Sediments - Crude oil and oil products are the most widespread environmental pollutants. The most efficient bioremediation is performed by using specific oil-degrading strains....  相似文献   

Comparison of selenium determination in liver samples by atomic absorption spectroscopy and inductively coupled plasma-mass spectrometry.     

Irina Rudik Miksa  Carol L Buckley  Nancy P Carpenter  Robert H Poppenga 《Journal of veterinary diagnostic investigation》2005,17(4):331-340

Selenium (Se) is an essential trace element that is often deficient in the natural diets of domestic animal species. The measurement of Se in whole blood or liver is the most accurate way to assess Se status for diagnostic purposes. This study was conducted to compare hydride generation atomic absorption spectroscopy (HG-AAS) with inductively coupled plasma-mass spectrometry (ICP-MS) for the detection and quantification of Se in liver samples. Sample digestion was accomplished with magnesium nitrate and nitric acid for HG-AAS and ICP-MS, respectively. The ICP-MS detection was optimized for 82Se with yttrium used as the internal standard and resulted in a method detection limit of 0.12 microg/g. Selenium was quantified by both methods in 310 samples from a variety of species that were submitted to the Toxicology Laboratory at New Bolton Center (Kennett Square, PA) for routine diagnostic testing. Paired measurements for each sample were evaluated by a mean difference plot method. Limits of agreement were used to describe the maximum differences likely to occur between the 2 methods. Results suggest that under the specified conditions ICP-MS can be reliably used in place of AAS for quantitation of tissue Se at or below 2 microg/g to differentiate between adequate and deficient liver Se concentrations.  相似文献   

Understanding forest owners’ preferences for policy interventions addressing mushroom picking in Catalonia (north-east Spain)     

Elena Górriz-Mifsud  Glòria Domínguez-Torres  Irina Prokofieva 《European Journal of Forest Research》2015,134(4):585-598

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Patterns of nodal metastases,biological behaviour and prognosis of canine mast cell tumours of the pinna: A multi-institutional retrospective study     

Carmit Chalfon  Riccardo Finotello  Silvia Sabattini  Irina Gramer  Joanna Siriol Morris  Marina Aralla  Emanuela Maria Morello  Erica Ferraris  Sofia Ramos  Gerry Polton  Luca Schiavo  Jane Dobson  Veronica Cola  Laura Marconato 《Veterinary and comparative oncology》2023,21(2):332-338

Canine cutaneous mast cell tumours (cMCTs) of the pinna have been associated with an aggressive biological behaviour, although data remain scarce. The knowledge acquired over the past years on histologic gradings, and the value of lymph node (LN) staging, may help in better characterizing this anatomical presentation. The first aim was to describe the frequency, location, and histologic appearance of LN metastases in cMCT of the pinna. A second aim was to evaluate prognosis. Medical records of dogs with cMCT of the pinna, that underwent tumour and sentinel (SLN) or regional LN (RLN) excision, were reviewed. The influence of potential prognostic variables on time to progression (TTP) and tumour-specific survival (TSS) was investigated. Thirty-nine dogs were included: 19 (48.7%) had Kiupel high-grade (K-HG) and 20 (51.3%) had low-grade (K-LG) MCTs. Eighteen (46.1%) dogs underwent SLN mapping: the superficial cervical LN was at least one of SLN in 17 (94.4%) cases. Twenty-two (56.4%) dogs had LN metastases; the superficial cervical LN was always involved. On multivariable analysis, only K-HG was associated with increased risk of progression (p = .043) and tumour-related death (p = .021). Median TTP and TSS were 270 and 370 days in K-HG, respectively; these were not reached in dogs with K-LG tumours (p < .01). cMCTs of the pinna are often K-HG and are also associated with a higher frequency of LN metastasis; however, we confirmed the independent prognostic value of histologic grading. A multimodal treatment may lead to favourable long-term outcome. Moreover, the superficial cervical LN is most often the SLN.  相似文献   

Latitudinal gradients in body size and sexual size dimorphism in fleas: males drive Bergmann's pattern     

Boris R. KRASNOV  Elena N. SURKOVA  Georgy I. SHENBROT  Irina S. KHOKHLOVA 《Integrative zoology》2023,18(3):414-426

We tested for the effects of latitude and geographic range size (GRS) on body size, leg length, and sexual size dimorphism (SSD) across 103 species of fleas, taking into account phylogenetic between-species relationships. When the data on body size were combined for males and females, the positive correlation between body size and latitude, but not GRS, was revealed. When the analysis was restricted to one sex only, the effect of latitude appeared to be non-significant for females, whereas male body size increased with an increase in latitude. Intraspecific body size variation was not associated with either the latitude or the latitudinal span of the geographic range, independently of which data subset was analyzed. No evidence of association between size-independent tibia length and latitude was found for either females, males, or both sexes combined. The degree of SSD decreased with a decrease in latitude but was not affected by GRS. We conclude that macroecological patterns might be manifested differently in males and females. This should be kept in mind while searching for these patterns.  相似文献   

Marine Fungal Cerebroside Flavuside B Protects HaCaT Keratinocytes against Staphylococcus aureus Induced Damage     

Ekaterina A. Chingizova  Ekaterina S. Menchinskaya  Artur R. Chingizov  Evgeny A. Pislyagin  Elena V. Girich  Anton N. Yurchenko  Irina V. Guzhova  Valery V. Mikhailov  Dmitry L. Aminin  Ekaterina A. Yurchenko 《Marine drugs》2021,19(10)

Cerebrosides are glycosylated sphingolipids, and in mammals they contribute to the pro-/anti-inflammatory properties and innate antimicrobial activity of the skin and mucosal surfaces. Staphylococcus aureus infection can develop, not only from minor scratches of the skin, but this pathogen can also actively promote epithelial breach. The effect of cerebroside flavuside B from marine sediment-derived fungus Penicillium islandicum (Aniva Bay, the Sea of Okhotsk) on viability, apoptosis, total caspase activity, and cell cycle in human epidermal keratinocytes HaCaT line co-cultivated with S. aureus, as well as influence of flavuside B on LPS-treated HaCaT cells were studied. Influence of flavuside B on bacterial growth and biofilm formation of S. aureus and its effect on the enzymatic activity of sortase A was also investigated. It was found S. aureus co-cultivated with keratinocytes induces caspase-depended apoptosis and cell death, arrest cell cycle in the G0/G1 phase, and increases in cellular immune inflammation. Cerebroside flavuside B has demonstrated its antimicrobial and anti-inflammatory properties, substantially eliminating all the negative consequences caused by co-cultivation of keratinocytes with S. aureus or bacterial LPS. The dual action of flavuside B may be highly effective in the treatment of bacterial skin lesions and will be studied in the future in in vivo experiments.  相似文献   

Distribution of lipids and fatty acids in the zooxanthellae and host of the soft coral Sinularia sp.     

Imbs  Andrey B.  Yakovleva  Irina M.  Pham  Long Q. 《Fisheries Science》2010,76(2):375-380

The lipid classes and the fatty acid (FA) compositions of the zooxanthellae, the host tissue, and intact coral were determined for the first time in a soft coral, Sinularia sp. The contents of monoalkyldiacylglycerol (MADAG), triacylglycerol, and polar lipids differed significantly between the zooxanthellae and the host fractions. The zooxanthellae were rich in polar lipids, whereas neutral lipids were concentrated in the host. MADAG comprised 35% of the host lipids and was practically absent in zooxanthellae. Hence, MADAG is only synthesized in animal tissues and serves as a biomarker for the host in the host–zooxanthellae association of these soft corals. Similar to the zooxanthellae of reef-building corals, the main FA in the zooxanthellae of Sinularia sp. were 18:4n-3, 20:5n-3, and 22:6n-3. In addition, 16:3n-4 and 16:4n-1 (8.9% in total) were found in these zooxanthellae. The ratios of 16:3n-4, 16:4n-1, 18:4n-3, 20:5n-3, and 22:6n-3 in the zooxanthellae to those in the host tissue were 4.2, 11.2, 10.1, 11.0, and 9.1, respectively. The proportions of some FA and lipid classes in the intact coral and its fractions showed that zooxanthellate lipids comprised 36 ± 15% of the total lipids in Sinularia sp. Two tetracosapolyenoic acids (24:5n-6 and 24:6n-3) are proposed as a biomarkers of the animal tissue and indicators of the purity of the zooxanthellae fractions from soft corals.  相似文献   

Early response of barley embryos to low- and high-dose gamma irradiation of seeds triggers changes in the transcriptional profile and an increase in hydrogen peroxide content in seedlings     

Polina Yu Volkova  Gustavo T. Duarte  Ludivine Soubigou-Taconnat  Elizaveta A. Kazakova  Stéphanie Pateyron  Vladimir S. Bondarenko  Sofia V. Bitarishvili  Ekaterina S. Makarenko  Roman S. Churyukin  Maria A. Lychenkova  Irina V. Gorbatova  Christian Meyer  Stanislav A. Geras’kin 《Journal of Agronomy and Crop Science》2020,206(2):277-295

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Black carbon decomposition and incorporation into soil microbial biomass estimated by C labeling     

Yakov Kuzyakov  Irina Subbotina  Irina Bogomolova 《Soil biology & biochemistry》2009,41(2):210-219

Incomplete combustion of organics such as vegetation or fossil fuel led to accumulation of charred products in the upper soil horizon. Such charred products, frequently called pyrogenic carbon or black carbon (BC), may act as an important long-term carbon (C) sink because its microbial decomposition and chemical transformation is probably very slow. Direct estimations of BC decomposition rates are absent because the BC content changes are too small for any relevant experimental period. Estimations based on CO₂ efflux are also unsuitable because the contribution of BC to CO₂ is too small compared to soil organic matter (SOM) and other sources.We produced BC by charring ¹⁴C labeled residues of perennial ryegrass (Lolium perenne). We then incubated this ¹⁴C labeled BC in Ah of a Haplic Luvisol soil originated from loess or in loess for 3.2 years. The decomposition rates of BC were estimated based on ¹⁴CO₂ sampled 44 times during the 3.2 years incubation period (1181 days). Additionally we introduced five repeated treatments with either 1) addition of glucose as an energy source for microorganisms to initiate cometabolic BC decomposition or 2) intensive mixing of the soil to check the effect of mechanical disturbance of aggregates on BC decomposition. Black carbon addition amounting to 20% of C_org of the soil or 200% of C_org of loess did not change total CO₂ efflux from the soil and slightly decreased it from the loess. This shows a very low BC contribution to recent CO₂ fluxes. The decomposition rates of BC calculated based on ¹⁴C in CO₂ were similar in soil and in loess and amounted to 1.36 10⁻⁵ d⁻¹ (=1.36 10⁻³% d⁻¹). This corresponds to a decomposition of about 0.5% BC per year under optimal conditions. Considering about 10 times slower decomposition of BC under natural conditions, the mean residence time (MRT) of BC is about 2000 years, and the half-life is about 1400 years. Considering the short duration of the incubation and the typical decreasing decomposition rates with time, we conclude that the MRT of BC in soils is in the range of millennia.The strong increase in BC decomposition rates (up to 6 times) after adding glucose and the decrease of this stimulation after 2 weeks in the soil (and after 3 months in loess) allowed us to conclude cometabolic BC decomposition. This was supported by higher stimulation of BC decomposition by glucose addition compared to mechanical disturbance as well as higher glucose effects in loess compared to the soil. The effect of mechanical disturbance was over within 2 weeks. The incorporation of BC into microorganisms (fumigation/extraction) after 624 days of incubation amounted to 2.6 and 1.5% of ¹⁴C input into soil and loess, respectively. The amount of BC in dissolved organic carbon (DOC) was below the detection limit (<0.01%) showing no BC decomposition products in water leached from the soil.We conclude that applying ¹⁴C labeled BC opens new ways for very sensitive tracing of BC transformation products in released CO₂, microbial biomass, DOC, and SOM pools with various properties.  相似文献   

Notch-mediated restoration of regenerative potential to aged muscle     

Conboy IM  Conboy MJ  Smythe GM  Rando TA 《Science (New York, N.Y.)》2003,302(5650):1575-1577

A hallmark of aging is diminished regenerative potential of tissues, but the mechanism of this decline is unknown. Analysis of injured muscle revealed that, with age, resident precursor cells (satellite cells) had a markedly impaired propensity to proliferate and to produce myoblasts necessary for muscle regeneration. This was due to insufficient up-regulation of the Notch ligand Delta and, thus, diminished activation of Notch in aged, regenerating muscle. Inhibition of Notch impaired regeneration of young muscle, whereas forced activation of Notch restored regenerative potential to old muscle. Thus, Notch signaling is a key determinant of muscle regenerative potential that declines with age.  相似文献