Isolation of equine herpesvirus-2 from the lung of an aborted fetus.     

Cecilia Mónica Galosi  Viviana Cid de la Paz  Leandro Carlos Fernández  Javier Pablo Martinez  María Isabel Craig  María Barrandeguy  María Elisa Etcheverrrigaray 《Journal of veterinary diagnostic investigation》2005,17(5):500-502

This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion.  相似文献   

Toward a multiplexed serotyping immunoassay for foot-and-mouth disease virus.     

Julie Perkins  Alfonso Clavijo  Josue I Ortiz  Timothy J Salo  Hilary J Holland  Benjamin J Hindson  Mary T McBride 《Journal of veterinary diagnostic investigation》2007,19(2):180-184

Initial results demonstrating the feasibility of a multiplexed liquid array immunoassay for foot-and-mouth disease viral antigen detection and simultaneous serotype differentiation are presented. Serotype-specific antibodies from rabbit and guinea pig hyperimmunesera were isolated and prepared for use in a multiplexed, bead-based assay. The performance of all of the available antibodies as both capture and detector reagents was evaluated in the multiplexed system to establish a combination exhibiting the highest homotypic responses and lowest heterotypic reactions. The multiplexed assay was evaluated against inactivated cell culture supernatant samples of the same subtype as the virus used to raise the capture and detector antibodies. Distinct serotype differentiation was observed, except in the case of serotype SAT1. Subsequently, cell culture supernatant samples from a larger pool of viral subtypes were analyzed. Distinct serotype differentiation was obtained when analyzing cell culture supernatant samples from viral serotypes C, Asia, and SAT3, irrespective of the subtype. However, limitations of the current antibody pairs were realized in some inconclusive results obtained when analyzing samples from a broader range of O, A, and SAT2 subtypes. The results obtained in this initial study will be used to further optimize the assay using polyvalent or monoclonal antibodies and move toward the analysis of clinical samples.  相似文献   

<Emphasis Type="Italic">Amaranthus leaf mottle virus</Emphasis>: 3′-end RNA sequence proves classification as distinct virus and reveals affinities within the genus <Emphasis Type="Italic">Potyvirus</Emphasis>     

Eduardo Segundo  Dietrich E. Lesemann  Germán Martín  María P. Carmona  Leticia Ruiz  Isabel M. Cuadrado  Leonardo Velasco  Dirk Janssen 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(1):81-87

Amaranthus leaf mottle virus (AmLMV) was classified as a member of the genus Potyvirus on the basis of its particle morphology, serology, and biological properties (Casetta et al., 1986). Based on these properties, an Amaranthus viridis-infecting virus isolated in Spain, causing mottle and leaf blistering as well as reduced growth has been identified as AmLMV. The 3′ terminal genomic region of this and a reference isolate from Italy has been sequenced and reveals a 95% nucleotide identity between the two isolates. The sequenced part comprises the coat protein with 281 amino acids and 315 nucleotides of the 3′ untranslated region (UTR) preceding a polyadenylated tail. Pairwise comparisons and phylogenetic analysis of the nucleotide and deduced amino acid sequences of the CP and 3′ UTR of the cloned cDNAs with those of other potyviruses shows that AmLMV is a distinct potyvirus closely related to Potato virus Y.  相似文献   

<Emphasis Type="Italic">Physalis ixocarpa</Emphasis> and <Emphasis Type="Italic">P. peruviana</Emphasis>, new natural hosts of <Emphasis Type="Italic">Tomato chlorosis virus</Emphasis>     

Helena P. Trenado  Isabel M. Fortes  Diamantina Louro  Jesús Navas-Castillo 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(2):193-196

Tomato chlorosis virus causes yellow leaf disorder epidemics in many countries worldwide. Plants of Physalis ixocarpa showing abnormal interveinal yellowing and plants of Physalis peruviana showing mild yellowing collected in the vicinity of tomato crops in Portugal were found naturally infected with ToCV. Physalis ixocarpa and P. peruviana were tested for susceptibility to ToCV by inoculation with Bemisia tabaci, Q biotype. Results confirmed that ToCV is readily transmissible to both species. The infection was expressed in P. ixocarpa by conspicuous interveinal yellow areas on leaves that developed into red or brown necrotic flecks, while P. peruviana test plants remained asymptomatic. Infected plants of both P. ixocarpa and P. peruviana served as ToCV sources for tomato infection via B. tabaci transmission. This is the first report of P. ixocarpa and P. peruviana as natural hosts of ToCV.  相似文献   

Three new homoisoflavanones from the bulbs of Ledebouria floribunda     

María Isabel Calvo   《Fitoterapia》2009,80(7):394-398

Three new homoisoflavanones, namely ledebourin A (1), ledebourin B (2) and ledebourin C (3) were isolated from the bulbs of Ledebouria floribunda. Their structures were elucidated on the basis of detail spectroscopic analyses. This is the first report of this type of homoisoflavanones. Ledebourin B (2) and ledebourin C (3) exhibited potent antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging compared to the positive controls, the well-known antioxidant BHT and BHA.  相似文献   

Giardia duodenalis in colony stray cats from Italy     

Isabel Guadano Procesi  Azzurra Carnio  Federica Berrilli  Margherita Montalbano Di Filippo  Alessia Scarito  Cristina Amoruso  Marco Barni  Marco Ruffini  Giulia Barlozzari  Manuela Scarpulla  Claudio De Liberato 《Zoonoses and public health》2022,69(1):46-54

Giardia duodenalis is the most common intestinal protozoan in humans and animals worldwide, including eight morphologically identical assemblages, infecting pets, livestock, wildlife and human beings. Assemblages A and B are those with the higher zoonotic potential, and they have been detected in several mammals other than humans; the others (C to H) show a higher host specificity. Cats can harbour both the specific Assemblage F and the zoonotic ones A and B. Several studies have been carried out on G. duodenalis genotypes in cats; however, the role of this species in the epidemiology of giardiasis is still poorly understood. In this scenario, the present study carried out the detection and genetic characterization at sub-assemblage level of G. duodenalis from colony stray cats in central Italy. In the period 2018–2019, 133 cat faecal samples were analysed for the presence of G. duodenalis cysts by a direct immunofluorescence assay. Positive samples were subsequently subjected to molecular analyses for assemblage/sub-assemblage identification. Forty-seven samples (35.3%) were positive for G. duodenalis cysts by immunofluorescence. G. duodenalis DNA was amplified at SSU-rDNA locus from 39 isolates: 37 were positive for zoonotic Assemblage A and 2 showed a mixed infection (A + B). Positive results for the β-giardin gene were achieved for 25 isolates. Sequence analysis revealed 16 isolates belonging to Sub-assemblage AII and 8 to Sub-assemblage AIII. One isolate resulted as ambiguous AI/AIII. Large sequence variability at the sub-assemblage level was detected, with several double peaks and mutations, making complex a proper isolate allocation. When compared with previous studies, the 35.3% prevalence of G. duodenalis in cats reported in the present article was surprisingly high. Moreover, all positive cats resulted to be infected with zoonotic assemblages/sub-assemblages, thus indicating stray cats as a possible source of human giardiasis and highlighting the sanitary relevance of cat colonies in the study area.  相似文献   

Differential expression of genes encoding cell wall proteins in vascular tissues from vertical and bent loblolly pine trees   总被引：1，自引：0，他引：1  

Zhang Y  Sederoff RR  Allona I 《Tree physiology》2000,20(7):457-466

Differential expression of cell wall proteins during plant development and in response to biotic or abiotic stress suggests that these proteins may contribute in different ways to plant cell wall architecture. Because the wood of loblolly pine (Pinus taeda L.) is highly specialized in the formation of secondary cell walls, it is an ideal tissue for studying these proteins. The cDNAs coding for six novel cell wall associated proteins, as well as a homologue for a phytocyanin, were identified and characterized from differentiating xylem of loblolly pine. Three of these cDNAs encoded new putative loblolly pine arabinogalactan proteins, based on their structural similarity to classical arabinogalactan proteins (AGPs). In addition, one clone was related to the proline-rich protein group and the other two to the glycine-rich protein group and the mussel adhesive protein. Relative expression of these genes was examined in different tissues and organs from normal trees (needles, phloem and vertical wood), the underside of bent trees (compression wood) and the lateral sides of the bent stems (side wood). All clones, except one, were highly expressed in vascular tissues with noticeable differences among the three types of wood. Their relationships and diversity provide the first insights into concerted expression and related function for this important group of proteins in cell wall formation of wood.  相似文献   

First report on Meloidogyne chitwoodi hatching inhibition activity of essential oils and essential oils fractions     

Jorge Miguel Silva Faria  Inês Sena  Bruno Ribeiro  Ana Margarida Rodrigues  Carla Maria Nobre Maleita  Isabel Abrantes  Richard Bennett  Manuel Mota  Ana Cristina da Silva Figueiredo 《Journal of pest science》2016,89(1):207-217

The Columbia root-knot nematode (CRKN), Meloidogyne chitwoodi, is an EPPO A2 type quarantine pest since 1998. This nematode causes severe damage in economically important crops such as potato and tomato, making agricultural products unacceptable for the fresh market and food processing. Commonly used nematicidal synthetic chemicals are often environmentally unsafe. Essential oils (EOs) may constitute safer alternatives against RKN. EOs, isolated from 56 plant samples, were tested against CRKN hatching, in direct contact bioassays. Some of the most successful EOs were fractionated and the hydrocarbon molecules (HM) and oxygen-containing molecules (OCM) fractions tested separately. 24 EOs displayed very strong hatching inhibitions (≥90 %) at 2 µL mL^?1 and were further tested at lower concentrations. Dysphaniaambrosioides, Filipendula ulmaria, Ruta graveolens, Satureja montana and Thymbra capitata EOs revealed the lowest EC₅₀ values (<0.15 µL mL^?1). The main compounds of these EOs, namely 2-undecanone, ascaridol, carvacrol, isoascaridol, methyl salicylate, p-cymene and/or γ-terpinene, were putatively considered responsible for CRKN hatching inhibition. S. montana and T. capitata OCM fractions showed hatching inhibitions higher than HM fractions. The comparison of EO and corresponding fractions EC₅₀ values suggests interactions between OCM and HM fractions against CRKN hatching. These species EOs showed to be potential environmentally friendly CRKN hatching inhibitors; nonetheless, bioactivity should be considered globally, since its HM and OCM fractions may contribute, diversely, to the full anti-hatching activity.  相似文献   

Edge type determines the intensity of forest edge effect on epiphytic communities     

Gregorio Aragón  Laura Abuja  Rocío Belinchón  Isabel Martínez 《European Journal of Forest Research》2015,134(3):443-451

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Correlation of selected constituents with the total antioxidant capacity of coffee beverages: influence of the brewing procedure     

López-Galilea I  De Peña MP  Cid C 《Journal of agricultural and food chemistry》2007,55(15):6110-6117

Relationships between volatile and nonvolatile compounds and the antioxidant capacity of coffee brews prepared from commercial conventional and torrefacto roasted coffees, employing commonly used doses and prepared by four brewing procedures (filter, plunger, mocha, and espresso machine) were assessed. Significant correlations between volatile Maillard reaction products and antioxidant capacity (measured by both 2,2-diphenyl-1-picrylhydrazyl radical and redox potential methods) were not observed. Highly positive correlations between browned compounds and caffeine with both antioxidant capacity parameters were reported. Principal component analysis allowed coffee brews separation according to coffee roasting processes (PC1) and brewing procedures (PC2), showing that in all cases coffee brews from torrefacto roasted coffee were more antioxidant that those extracted from conventional ones; also, coffee brews extracted by an espresso machine were more antioxidant than those extracted by mocha, plunger, and filter machines.  相似文献