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41.
Summary Chromosome number of morphogenic and non-morphogenic calli and regenerated plants of barley were determined. Cultures were obtained from two kinds of explants, immature embryos and seedling leaves from three cultivars, Ingrid, Dissa and Golden Promise. Callus chromosome analyses were carried out during a 12 month period in a medium containing 2 mg/l of 2,4-D. Diploid cells were predominant in all cases; although in leaf-derived cultures, retraploid cells (2n=4x=28) showed a tendency to increase as time in culture increased and after more than six months in culture, diploid cells decreased to percentages of almost 70%. Aneuploid cells were generally infrequent in all cases. The source of explant has been more important than the genotype (cultivar) and the type of callus (morphogenic vs. non-morphogenic) in the chromosomal stability of cultures as time increases. From short term cultures, only 1.85% of the regenerated plants were tetraploid, the remaining were diploids. The ability of morphogenic calli to regenerate plants decreased before any significant reduction of diploid cells were observed. 相似文献
42.
María Valeria Romagnoli Juan Pablo A. Ortiz Gerardo D. Cervigni Cintia Heisterborg Rubén H. Vallejos 《Euphytica》1996,90(1):89-93
Summary Somatic embryos of genotype R11 of the alfalfa variety Pampeana were produced from embryogenic calli derived from leaf sections. They were induced by an auxin shock and its development was attempted on six different media. The best condition for somatic embryo production was inducing callus on MS medium plus 10 M 2,4-D and 4,6 M KIN and transferring them, after the auxin shock, to MS with 10–20 mM NH4
+ and 30 mM proline. More than 500 somatic embryos per plate were produced. Embryos were grown to plants on MS or half strength MS media and all regenerated plants resembled the original R11 genotype. This technique could be useful in alfalfa Pampeana improvement using genetic modification. 相似文献
43.
RAPD genetic diversity of Albanian olive germplasm and its relationships with other Mediterranean countries 总被引:4,自引:0,他引:4
Angjelina Belaj Zlatko Satovic Hajri Ismaili Dhimitër Panajoti Luis Rallo Isabel Trujillo 《Euphytica》2003,130(3):387-395
RAPD markers were used for the study of 19Albanian olive cultivars and two wild olives (oleasters). A total of 76polymorphic
bands (4.8 polymorphic markers per primer) out of 107 reproducible were obtained using 16 primers. The number of bands per
primer ranged from 4 to 10,whereas the number of polymorphic bands ranged from 1 to 9, corresponding to 71%of the total amplification
products. All the accessions could be identified by the combination of four primers: OPA-19;OPA-02; OPK-16 and OPP-19. The
dendrogram,based on Jaccard's index, included three major groups according to their origin: 1)most of the cultivars from the
area of Berat (South of Albania) 2) cultivars from the Centre and Centre-North of Albania and3) cultivars from the Centre
and North-West of Albania along with the oleaster from Elbasan. In order to evaluate the origin of Albanian cultivars they
were compared to those diffused in other countries like Greece, Italy and Turkey, due to geographical and historical affinity
among these countries, by using a one way AMOVA. Although most of the genetic diversity was attributable to differences among
cultivars within each country (91.47%) significantφ-values among countries(φst = 0.085; p < 0.001)suggested the existence of RAPD phenotypic differentiation. Significant φ-values in all pairs formed by Albania with
the other countries were observed. These results are consistent with the autochthonous origin of Albanian cultivars.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
44.
Ram C. Sharma G. Ortiz-Ferrara J. Crossa M. R. Bhatta M. A. Sufian J. Shoran A. K. Joshi R. Chand Gyanendra Singh R. Ortiz 《Euphytica》2007,157(3):457-464
Improving the level and stability of grain yield is the primary objective of wheat breeding programs in the Eastern Gangetic
Plains (EGP) of South Asia. A regional wheat trial, the Eastern Gangetic Plains Yield Trial (EGPYT), was initiated by CIMMYT
in collaboration with national wheat research programs in Bangladesh, Nepal, and India in 1999–2000 to identify wheat genotypes
with high and stable grain yield, disease resistance, and superior agronomic traits for the EGP region. A set of 21 wheat
experimental genotypes selected from a regional wheat screening nursery in South Asia, three improved widely grown cultivars
(Kanchan, PBW343 and Bhrikuti), and one long-term cultivar (Sonalika) were tested at 9–11 sites in six wheat growing seasons
(2000–2005) in the EGP. The 21 experimental genotypes were different in each year, whereas the four check cultivars were common.
In each year, one or more of the experimental genotypes showed high and stable grain yield and acceptable maturity, plant
height, and disease resistance compared to the check cultivars. Three improved cultivars have already been commercially released
in the region through EGPYT and many germplasm lines have been used in the breeding programs as parents. Identification of
wheat genotypes with high-grain yield in individual sites and high and stable yield across the EGP region underlines their
value for regional wheat breeding programs attempting to improve grain yield and agronomic performance. 相似文献
45.
Summary The production of 2n pollen (pollen with the sporophytic chromosome number) was evaluated in 4x and 6x taxa of Vaccinium section Cyanococcus. Mean frequencies of 2n pollen producers were 17.1% and 8.3% in natural 4x and 6x populations, respectively. The frequency of 2n pollen producers in the 4x species ranged from 8.6% (V. angustifolium) to 23.8% (V. pallidum). Level of 2n pollen production was genotypically variable (1% to 37.4%). The widespread occurrence of 2n pollen in 2x, 4x and 6x taxa suggests that sexual polyploidization was widespread and responsible for the origin of the polyploid species found in this genus. The frequency of 2n pollen producers was not significantly different between the 4x species and their putative 2x ancestors. These results support the origin of 4x and 6x taxa as a consequence of sexual polyploidization. Polyploids derived from sexual polyploidization would be expected to have increased fitness and flexibility due to the mode of 2n pollen formation. In blueberry species the predominant mode of 2n pollen formation is genetically equivalent to a first division restitution mechanism (FDR). FDR 2n pollen transmits a high percentage of the heterozygosity and a large fraction of the epistasis from the 2x parent to the 4x offspring. 相似文献
46.
Summary Improvement of durum wheat at the International Maize and Wheat Improvement Center (CIMMYT) was achieved by exploiting the variability of the tetraploid species in large numbers of combinations, and multi-location testing of germplasm on a world wide base. High yielding, widely adapted durums with short straw, good fertility, and adequate earliness were selected for distribution to major durum growing countries. Present attempts to improve durum wheat are directed at adjusting agronomic traits such as leaf angle and straw strength, and increasing spike fertility and spike size, thereby reaching higher grain numbers per unit area and higher yield potential. Diversification of the developmental pattern and incorporation of drought tolerance aim at further widening adaptation. 相似文献
47.
Ovejero-López I Haahr AM van den Berg F Bredie WL 《Journal of agricultural and food chemistry》2004,52(26):8119-8126
Flavor release from a mint-flavored chewing gum model system was measured by atmospheric pressure chemical ionization mass spectroscopy (APCI-MS) and sensory time-intensity (TI). A data analysis method for handling the individual curves from both methods is presented. The APCI-MS data are ratio-scaled using the signal from acetone in the breath of subjects. Next, APCI-MS and sensory TI curves are smoothed by low-pass filtering. Principal component analysis of the individual curves is used to display graphically the product differentiation by APCI-MS or TI signals. It is shown that differences in gum composition can be measured by both instrumental and sensory techniques, providing comparable information. The peppermint oil level (0.5-2% w/w) in the gum influenced both the retronasal concentration and the perceived peppermint flavor. The sweeteners' (sorbitol or xylitol) effect is less apparent. Sensory adaptation and sensitivity differences of human perception versus APCI-MS detection might explain the divergence between the two dynamic measurement methods. 相似文献
48.
Rodomiro Ortiz Birgitte Lund Sven-Bode Andersen 《Genetic Resources and Crop Evolution》2003,50(5):455-459
Phenotypic diversity among 75 Nordic spring wheat cultivars was assessed in a glasshouse experiment, in which plots had no fertilizer or received 14-3-23 NPK plus Mg. On average, the fertilizer application delayed flowering by one day, shortened plant height (PH), as well as enhanced the number of fertile tillers (NFT), fresh and dry straw weight (FSW and DSW, respectively), but influenced negatively the dry matter content in the straw (DMCS) and the number of kernels per spike (K/S). The cultivar-by-fertilizer interaction did not affect significantly days to flowering (DF), PH, FSW, DSW, DMCS, thousand kernel weight, and K/S. Only NFT was significantly affected by this interaction. There were significant differences among cultivars for all characteristics, and the breeding gains were significant for improving earliness (as determined by DF), shortening PH, as well as for reducing DSW and DMCS irrespective of the environment. On average, FSW was low in newer cultivars grown in high inputs, while NFT was low in newer cultivars when grown in low inputs. The country of origin affected significantly DF, PH, DMCS, K/S, and 1000-kernel weight. On average, cultivars developed for Finland and Norway were significantly taller, and had higher DMCS. Finnish cultivars also flowered 1 or 2 days earlier and showed higher K/S than cultivars adopted in southern Scandinavia or Norway. 相似文献
49.
A heat-induced degradation was carried out over two crude paprika oleoresins diluted with high oleic or high linoleic oil. Occurrence of oxygen was avoided, and changes in individual carotenoids were followed over time. Degradation rate constants were not significantly different, so that carotenoid stability was not linked with unsaturation degree of the oily system. A parallel reaction to degradation, trans to cis carotenoid isomer conversion, was also denoted during the thermal treatment, and it initially showed a higher rate than degradation of cis-isomers. Both processes (isomerization and cis-isomers degradation) were finally compensated, and their development was also unaffected by the nature of the lipid profile. Under the reaction conditions, oleic and linoleic fatty acids showed the same reactivity and induced degradative reactions, equally affecting the carotenoid profile. An enhanced stability of carotenoid content and provitamin A value was not achieved with a decrease in the unsaturation level of the oily system. 相似文献
50.
Helianthinin, the main storage protein of sunflowers, has low water solubility and does not form a gel when heated; this behavior is different from other 11S globulins and limits its food applications. To understand this particular behavior, changes on helianthinin association-dissociation state induced by modifications in pH and ionic strength were analyzed. The influence of these different medium conditions on its thermal stability and tendency to form aggregates was also studied. Helianthinin behavior at different pH values and ionic strengths is similar to other 11S globulins except that it remains in a trimeric form at pH 11. Helianthinin thermal stability is higher than other 11S globulins but is lower than oat 11S globulin. Alkaline pH produces a 10 degrees C decrease of its denaturation temperature and also of the cooperativity of denaturation process, but it does not affect the denaturation activation energy. The decrease in thermal stability with the pH increase is also manifested by its tendency to form aggregates by SH/SS interchange reactions. When thermal treatments at alkaline pH are performed, all helianthinin subunits form aggregates, characterized by a higher proportion of beta-polypeptides than alpha-polypeptides, which is an indication that aggregation is accompanied by dissociation. Treatments at 80 degrees C are sufficient to induce aggregation but not to produce denaturation, and in these conditions hexameric forms remain after the treatment. 相似文献