Antiproliferative activity of violaxanthin isolated from bioguided fractionation of Dunaliella tertiolecta extracts     

Pasquet V  Morisset P  Ihammouine S  Chepied A  Aumailley L  Berard JB  Serive B  Kaas R  Lanneluc I  Thiery V  Lafferriere M  Piot JM  Patrice T  Cadoret JP  Picot L 《Marine drugs》2011,9(5):819-831

Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 μg·mL⁻¹ (0.17 μM). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 μg·mL⁻¹ (13.3 μM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 μg·mL⁻¹ (66.7 μM). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained.  相似文献   

Measurement of the puncture profile and extradural pressure of cattle during extradural anaesthesia     

Isabelle Iff Dr med vet  Diplomate ECVAA    Sonja Franz† Dr med vet  & Yves Moens Dr med vet  PhD  Diplomate ECVAA 《Veterinary anaesthesia and analgesia》2009,36(5):495-501

ObjectiveTo measure the pressure profile during caudal extradural puncture and subsequent extradural anaesthesia in cattle and to investigate the presence of extradural pressure waves.Study designProspective experimental study.AnimalsEleven cattle aged 4.1 ± 2.5 years (range 0.8 to 8.8 years), with a body weight of 613 ± 162 kg (range 302–840 kg).MethodsCaudal extradural puncture was performed. To measure the extradural pressure profile, the needle was connected to an electronic pressure transducer placed at the height of the base of the tail. The pressure profile was recorded for 3 minutes following extradural puncture. Lack of resistance to injection of saline was assessed. One minute and 10 minutes after extradural anaesthesia with procaine extradural pressure was recorded. Correct extradural needle placement was assessed by clinical response.ResultsThree minutes after extradural puncture the median pressure was ?16 (range ?25 to 25) mmHg. Pressure in the extradural space 1 minute after the lack of resistance, 3 seconds after injection, and 10 minutes after injection was ?15 (?24 to 33) mmHg, 8 (?17 to 84) mmHg, and ?7 (?25 to 27) mmHg respectively. Pressure waves were visible after puncture, after lack of resistance, 3 seconds and 10 minutes after injection, in 4, 6, 8 and 7 cattle respectively. Pressure after testing lack of resistance, after the injection of local anaesthetic, as well as at the end of the measurement, period was significantly higher than baseline. All cattle showed clinical signs indicative of successful extradural needle placement.Conclusion and clinical relevance Extradural pressure was sub-atmospheric in 82% of the animals. Pressure waves were not consistently present before or after extradural injection, which limits their usefulness to confirm correct extradural needle placement. Extradural pressures increase significantly after injection of local anaesthetic solution. However, the clinical significance of the increase in extradural pressures was not clear.  相似文献   

Recombinant proteins and peptides as tools for studying IgE reactivity with low-molecular-weight glutenin subunits in some wheat allergies     

Snégaroff J  Branlard G  Bouchez-Mahiout I  Laudet B  Tylichova M  Chardot T  Pecquet C  Choudat D  Raison-Peyron N  Vigan M  Kerre S  Laurière M 《Journal of agricultural and food chemistry》2007,55(24):9837-9845

Two genes of wheat low-molecular-weight glutenin subunits (LMW-GS), B16 and P73, were cloned and expressed in E. coli. They were homologous to proteins encoded respectively at Glu-B3 and Glu-D3 loci. The N-terminal and C-terminal halves of B16 (NB16 and B16C) and the two chimeras combining the halves of the two genes (B16-P73 and P73- B16) were also expressed. All these constructs were compared for their reactivity with IgE from 24 patients suffering from different forms of wheat allergies. The results confirmed that LMW-GSs bound IgE in all adult allergies tested. Strong differences in reactivity between all the constructs were observed. They were disease-dependent. In wheat-dependent exercise-induced anaphylaxis (WDEIA), the reactivity of the constructs depended partly on common epitopes with omega-5 gliadins but also on differences in molecule conformation. The presence of NB16 in the constructs greatly influenced their IgE reactivity.  相似文献   

Ethyl hexanoate transfer modeling in carrageenan matrices for determination of diffusion and partition properties     

Juteau-Vigier A  Atlan S  Deleris I  Guichard E  Souchon I  Trelea IC 《Journal of agricultural and food chemistry》2007,55(9):3577-3584

Aroma compound properties in food matrices, such as volatility and diffusivity, have to be determined to understand the effect of composition and structure on aroma release and perception. This work illustrates the use of mass transfer modeling to identify diffusion and partition properties of ethyl hexanoate in water and in carrageenan matrices with various degrees of structure. The comparison of results obtained with a diffusive model to those obtained with a convective model highlights the importance of considering the appropriate transfer mechanism. Modeling of the preliminary experimental steps ensures correct estimation of the conditions for the main aroma release step. The obtained values of partition and diffusion coefficients are in agreement with those found in the literature (either experimentally determined or predicted by theoretical equations) and demonstrate that the structure level of carrageenan matrices has little influence on diffusion properties of ethyl hexanoate (less than 20%).  相似文献   

New indicators of beef sensory quality revealed by expression of specific genes   总被引：4，自引：0，他引：4  

Bernard C  Cassar-Malek I  Le Cunff M  Dubroeucq H  Renand G  Hocquette JF 《Journal of agricultural and food chemistry》2007,55(13):5229-5237

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DYNAMIC MAGNETIC RESONANCE IMAGING OF THE NORMAL CANINE PITUITARY GLAND     

John P.  Graham  MVB  MSc  Gregory D.  Roberts  DVM  MS  Susan M.  Newell  DVM  MS 《Veterinary radiology & ultrasound》2000,41(1):35-40

The pituitary glands of six normal dogs were evaluated using dynamic magnetic resonance imaging. T1 weighted images were obtained every 13 seconds for three minutes of three contiguous slices through the pituitary gland following a bolus intravenous injection of gadolinium-DTPA. Contrast enhancement was seen initially in the region of the pituitary stalk at 52-65 seconds followed by uniform enhancement at 104-143 seconds post injection. This pattern of enhancement was seen in all subjects and is similar to that reported in humans.  相似文献   

Sensitivity distribution of Septoria tritici isolates to flusilazole for consecutive years 1993, 1994 and 1995 in France,Germany and the United Kingdom     

Richard Power  Tom McHale  Isabelle Gasnier  Jean-Luc Genet  Ronald Hamlen 《Pest management science》1998,54(3):258-260

Sensitivities of Septoria tritici populations to flusilazole were stable across Europe during the three seasons, 1993–1995 inclusive. © 1998 Society of Chemical Industry  相似文献   

Evaluation of B-lines with 2 point-of-care lung ultrasound protocols in cats with radiographically normal lungs     

Manon Rigot DMV  IPSAV  Søren R. Boysen DVM  DACVECC  Isabelle Masseau DMV  PhD  DACVR  Jo-Annie Letendre DMV  DACVECC 《Journal of Veterinary Emergency and Critical Care》2024,34(2):143-152

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