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Anton I Kovács K Fésüs L Várhegyi J Lehel L Hajda Z Polgár JP Szabó F Zsolnai A 《Acta veterinaria Hungarica》2008,56(2):181-186
The objective of this study was to estimate the effect of the thyroglobulin (TG) locus on beef quality traits in some beef cattle breeds and to investigate the effect of the DGAT1 locus on milk production traits in the Hungarian Holstein Friesian population. TG and DGAT1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. At the TG locus TT bulls showed the highest fat percentage values in the longissimus dorsi muscle (m. longissimus dorsi); the difference between CC and TT genotypes was significant. DGAT1 GC/GC cows had the highest milk, fat and protein yield values. Due to the relatively small number of GC/GC cows the difference proved to be significant only between AA/AA and AA/GC genotypes. 相似文献
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Makrai L Kobayashi A Matsuoka M Sasaki Y Kakuda T Dénes B Hajtós I Révész I Jánosi K Fodor L Varga J Takai S 《Veterinary microbiology》2008,131(3-4):318-323
Rhodococcus equi has been isolated from the submaxillary lymph nodes of domesticated pigs, but little is known about the presence of R. equi in wild boars. The aim of the study was the evaluation of the incidence of R. equi in wild boars and the characterisation of them. Of 482 submaxillary lymph nodes of wild boars shot in 39 settlements throughout Hungary, R. equi was isolated from 60 specimens, and plasmid types of 82 isolates were examined. The isolates were tested for the presence of 15-17-kDa (VapA) and 20-kDa virulence-associated protein antigen (VapB) genes by polymerase chain reaction (PCR). Plasmid DNAs were isolated and analysed by digestion with restriction endonucleases to estimate size and compare their polymorphisms. None of the 82 isolates contained vapA gene but 21 isolates (25.6%) were positive for vapB gene showing 827bp product of the expected size in the PCR amplification. Sixty-one strains (74.4%) did not contain plasmid. The 21 isolates of intermediate virulence contained virulence plasmids that were identified as types 1 (1 isolate), 5 (16 isolates), 21 (1 isolate), and three new distinct plasmid variants (1-1-1 isolate), respectively. On the basis of restriction digestion patterns of plasmid DNAs, we tentatively designated the new variants as types 25-27, respectively. The prevalence of R. equi strains of intermediate virulence among the isolates originated from the submaxillary lymph nodes of wild boars (25.6%) is very similar to those of domestic pigs (26.8%) in Hungary, and plasmid type 5 is the predominating one in both groups. This is the first report of isolation of VapB-positive R. equi from wild boars in the world. 相似文献
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Tibor Farkas István Csengeri Ferenc Majoros János Oláh 《Aquaculture (Amsterdam, Netherlands)》1978,14(1):57-65
Carp, weighing 15–25 g, were maintained in the laboratory on a control diet, or on diets prepared from wheat or corn, and the incorporation of sodium (1?14C)-acetate into their liver total fatty acids as well as into liver triglycerides and phospholipids was followed. The control diet was characterized by a high concentration of total lipids and linolenic acid, and the other two feeds by a low concentration of total lipids including linolenic acid and high concentration of carbohydrates. The highest rate of fatty acid biosynthesis was observed in fish given the corn diet, and the lowest rate was found in the carp on the control diet. Levels of radioactivity became very high in triglycerides of corn-fed fish. The major products of fatty acid biosynthesis were palmitic and oleic acids, in the corn-fed fish, while in the control fish almost 50% of the total radioactivity was in the long chain polyunsaturated fatty acids. The degree of labeling of oleic and palmitic acids appeared to depend on the level of linolenic acid in the diet. The results suggest that about 1% of linolenic acid in the diet is required to keep lipogenesis at a low level as well as to prevent hyperproduction of oleic acid. Linoleic acid seems to play a subordinate role in regulating lipogenesis in carp liver. 相似文献
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Berghuis P Rajnicek AM Morozov YM Ross RA Mulder J Urbán GM Monory K Marsicano G Matteoli M Canty A Irving AJ Katona I Yanagawa Y Rakic P Lutz B Mackie K Harkany T 《Science (New York, N.Y.)》2007,316(5828):1212-1216
The roles of endocannabinoid signaling during central nervous system development are unknown. We report that CB(1) cannabinoid receptors (CB(1)Rs) are enriched in the axonal growth cones of gamma-aminobutyric acid-containing (GABAergic) interneurons in the rodent cortex during late gestation. Endocannabinoids trigger CB(1)R internalization and elimination from filopodia and induce chemorepulsion and collapse of axonal growth cones of these GABAergic interneurons by activating RhoA. Similarly, endocannabinoids diminish the galvanotropism of Xenopus laevis spinal neurons. These findings, together with the impaired target selection of cortical GABAergic interneurons lacking CB(1)Rs, identify endocannabinoids as axon guidance cues and demonstrate that endocannabinoid signaling regulates synaptogenesis and target selection in vivo. 相似文献
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Anwair MA Károlyházy L Szabó D Balogh B Kövesdi I Harmat V Krenyácz J Gellért A Takács-Novák K Mátyus P 《Journal of agricultural and food chemistry》2003,51(18):5262-5270
Ten pairs of pyridazinone regioisomers were prepared, and their lipophilicity was described by the logarithm of the octanol/water partition coefficient (log P) determined experimentally and calculated with prediction methods. The 4- and 5-(substituted amino)-3(2H)-pyridazinone regioisomers were synthesized by nucleophilic substitution of one of the chloro atoms of 4,5-dichloro-2-methyl-3(2H)-pyridazinone or its 6-nitro derivative. Structures of new compounds were proven by spectroscopic methods. The experimental log P values were obtained by a shake flask method in octanol and a S?rensen buffer (pH 7.4) solvent system. A consequent difference was found in the lipophilicity of regioisomers. For each isomer pair, the log P value of the 4-isomer was significantly (average by 0.75 log unit) higher than that of the 5-isomer. Some quantum chemical calculations as well as X-ray analysis of two pairs of regioisomers were also carried out to gain insight into the structural differences of regioisomers. The log P values were calculated by the fragmental approach KOWWIN and a QSPR analysis (3DNET). The a priori KOWWIN gave poor agreement, but with the programs KOWWIN with EVA (experimental value adjusted) and 3DNET, the results were generally in agreement with experiment. 相似文献