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241.
242.
The present study was designed to investigate the physicochemical properties of canine alpha-1-acid glycoprotein (AGP), and to establish a method for measuring serum AGP in canine. Fifty-three normal beagles were used in the study. AGP was purified from normal canine sera by successive ammonium sulfate precipitation, ion-exchange chromatography and gel filtration. The serum AGP concentration was measured by single radial immunodiffusion (SRID). Canine AGP had a molecular weight of 42,000 +/- 2,000 Da and contained 40.9% carbohydrate. Gel isoelectric focusing revealed microheterogeneity with 6-7 bands in a pI range (isoelectric points) of 3.2-3.8. AGP migrated to the alpha(1)-globulin region on immunoelectrophoresis. The serum AGP level of 35 normal beagles was 283.4 +/- 113.3 mug/ml. Canine AGP was isolated, and its physicochemical properties were clarified. SRID may be a useful method for quantification of serum AGP in canine. 相似文献
243.
Hisanori Itoh Yasushi Hara Natsuko Yoshimi Yasuji Harada Yoshinori Nezu Takuya Yogo Hiroki Ochi Daisuke Hasegawa Hiromitsu Orima Masahiro Tagawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(7):701-706
We investigated the epidemiological characteristics of intervertebral disc herniation (IVDH) in Japan in a large population using a retrospective study. The sample population was dogs (n=297) with IVDH in Japan. Medical records were reviewed for breed, sex, age, affected interspace and neurological severity. The dogs were comprised of 132 cases of cervical IVDH (C-IVDH) and 165 cases of thoracolumbar IVDH (TL-IVDH). In Japan, the Dachshund, Beagle and Shih Tzu tended to suffer from both C-IVDH and TL-IVDH. The Shiba Inu, a characteristic Japanese dog breed, suffered from both C-IVDH and TL-IVDH, although there was little data relating to the whole breed. Male dogs tended to suffer from C-IVDH and TL-IVDH at a rate almost twice that of females in Japan. Among the three predominant dog breeds, the Dachshund, Beagle and Shih Tzu, the Dachshund tended to suffer from both C-IVDH and TL-IVDH at an earlier age than the Beagle, and the Beagle tended to suffer from both C-IVDH and TL-IVDH earlier than the Shih Tzu. Among the three predominant breeds, the Shih Tzu, in particular, tended to suffer from both C-IVDH and TL-IVDH at multiple sites. Our data from Japan were in partial agreement with previous data from the U.S.A., and epidemiological characteristics of IVDH peculiar to Japan were also identified. 相似文献
244.
Itoh T Kojimoto A Nibe K Uchida K Shii H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):61-63
An 11-year-old male Golden Retriever presented with progressive weight loss, tachycardia, hyperthermia, polyuria and polydipsia. A freely movable mass, 4.5 x 4 cm in size, was palpated at the cranioventral cervical region. Hormonal study revealed high levels of serum thyroid hormones, and a tentative diagnosis of hyperthyroidism due to a thyroid tumor was made. The tumor was removed surgically and diagnosed histopathologically as thyroid gland adenoma. Serum thyroid hormone levels decreased after surgery with improved clinical signs. At 12 months after surgery, the dog maintained a good physical condition with no evidence of recurrence. 相似文献
245.
Wakaiki S Maehara S Abe R Tsuzuki K Igarashi O Saito A Itoh N Yamashita K Izumisawa Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):465-470
In dogs, a variety of diseases of the retina and choroid have been reported, either separately or concomitantly; however, the canine choroid is difficult to evaluate by veterinary techniques currently available. Indocyanine green (ICG) angiography is widely used in human ophthalmology, but has not been investigated for use in canine ophthalmology. The aim of this study was to apply a new approach to ICG angiography and compare the resulting angiograms with fluorescein (FLUO) angiograms of the ocular fundus in dogs. With a fundus camera equipped with an infrared-sensitive charged coupled device (CCD), we performed angiography on eight healthy beagles under inhalation anesthesia. ICG angiography enabled clear visualization of the choroidal vasculature, whereas FLUO angiography showed only the retinal vessels. At 8.4 +/- 3.6 sec after administration of ICG dye into the cephalic vein, the choroidal arteries could be seen extending radially from the optic disc, then the choroidal veins became apparent at 10.2 +/- 4.1 sec, coursing alongside the choroidal arteries. Gradual fading of the choroidal vessels began 13.2 +/- 2.2 min after the dye was administered, and overall diffuse fluorescence of the fundus appeared. Diffuse fluorescence of the fundus continued after the choroidal vessels and optic disc faded at about 58.3 +/- 5.3 min from administration of the dye. In conclusion, ICG angiography provides clear resolution and is reliable and simple, thus offering promise as a diagnostic aid for clinical evaluation of the choroid in dogs. 相似文献
246.
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248.
Akira Itoh Tatsuhiro Ohkubo Satoshi Nanami Sylvester Tan Takuo Yamakura 《Forest Ecology and Management》2010
Habitat difference is an important mechanism for maintenance of tree diversity in tropical forests. The first step in studies of habitat difference is to statistically analyze whether the spatial distributions of tree populations are skewed to species-specific habitats; this is called a habitat association test. We propose a novel habitat association test on the basis of the probability of tree occurrence along a continuous habitat variable. The test uses torus shift simulations to obtain a statistical significance level. We applied this test to 55 common dipterocarp species in a 52-ha plot of a Bornean forest to assess habitat associations along an elevation gradient. The results were compared to those of three existing habitat association tests using the same torus shift simulations. The results were considerably different from one another. In particular, the results of two existing tests using discrete habitat variables varied with differences in habitat definitions, specifically, differences in elevation break points, and the number of habitat classes. Thus, definitions of habitats must be taken into account when habitat association tests with discrete habitat variables are used. Analyses of artificial populations independent of habitat showed that all of the tests used were robust with respect to spatial autocorrelation in tree distributions, although one existing test had a higher risk of Type I errors, probably due to the use of multiple tests of significance. Power analysis of artificial populations in which distributions were skewed to certain elevations showed that the novel test had comparable statistical power to the most powerful existing test. Statistical power was affected not by the total number of a given tree but by the number of clumps in a plot, suggesting that >5 clumps were required for a reliable result. 相似文献
249.
Yuki Tobimatsu Toshiyuki Takano Hiroshi Kamitakahara Fumiaki Nakatsubo 《Journal of Wood Science》2010,56(3):233-241
It is known that the conventional dehydrogenative polymerization of sinapyl alcohol (S-alc) gave syringyl synthetic lignins
(S-DHPs), but in extremely low yields. In this article, to examine the contribution of syringyl quinone methide intermediates
(S-QM) on S-DHP production, horseradish peroxidase (HRP)-catalyzed dehydrogenative polymerization of S-alc was carried out
in the presence of nucleophilic reagents that promote the rearomatization of S-QM. First, the HRP-catalyzed polymerization
of sinapyl alcohol γ-O-β-D-glucopyranoside (isosyringin, iso-S), which allows us to monitor the polymerization process in a homogeneous aqueous phase,
was utilized for screening of a nucleophile used as an S-QM scavenger. Monitoring of iso-S polymerization in the presence
of various nucleophilic reagents by UV spectroscopy and gel permeation chromatography with photodiode array detection (GPC-PDA)
revealed a high ability of azide ion to convert oligomeric S-QM efficiently to S-DHP. Accordingly, azide ion was utilized
as an S-QM scavenger in HRP-catalyzed polymerization of S-alc, which resulted in high-yield production of S-DHPs (∼83%), as
expected. The 1H-, 13C-, and 2D-HSQC NMR investigations on the resulting S-DHPs clearly demonstrated that azide ion efficiently performed nucleophilic
additions to the C-α of S-QM during the polymerization. These results provide experimental proof that the low reactivity of S-QM with nucleophiles
(such as water, phenolic, and aliphatic hydroxyl groups) in the conventional polymerization system critically impedes the
production of S-DHPs from S-alc. 相似文献
250.
Four regions of chloroplast DNA were sequenced as a prospective genetic marker to identify Japanese representatives of Cyclobalanopsis: Quercus acuta, Q. sessilifolia, Q. salicina, Q. myrsinaefolia, Q. glauca, and Q. gilva. We found that Q. gilva was distinguished from other species based on both the trnL-trnF and trnT-trnL intergenic spacers. The evidence shows good coincidence with the fact that Q. gilva has several peculiar morphological features distinguishable from those of other species. There was no difference in trnL intron and matK. Both trnT-trnL and trnL-trnF intergenic spacers are capable of being used as genetic markers to identify Q. gilva among Cyclobalanopsis species.Part of this paper was presented at the 48th annual meeting of the Japan Wood Research Society, Shizuoka, April 3–5, 1998 相似文献