cDNA cloning of two types of glutamate dehydrogenase from a reproductively sterile mutant of Ulva pertusa (Chlorophyta) grown under different thermal conditions     

Makoto Kakinuma  Yoshitada Kozawa  Sayaka Itoh  Hideomi Amano 《Fisheries Science》2001,67(2):380-382

  相似文献   

Anaesthetic and cardiopulmonary effects of balanced anaesthesia with medetomidine-midazolam and butorphanol in dogs     

Itamoto K  Hikasa Y  Sakonjyu I  Itoh H  Kakuta T  Takase K 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2000,47(7):411-420

The anaesthetic and cardiopulmonary effects of combinations of medetomidine (Me), midazolam (Mi) and butorphanol (Bu) were evaluated in dogs. The characterization of anaesthetic effects was assessed using a scoring system. The combinations tested were 20 or 40 micrograms/kg Me and 0.5 mg/kg Mi (20Me-Mi or 40Me-Mi) followed by either an intravenous injection of physiological saline solution (PSS) or Bu (0.1 or 0.3 mg/kg). The mixture of Me and Mi was injected intramuscularly, followed 15 min later by an intravenous injection of Bu or PSS in all six groups. The combined Me-Mi induced deep sedation but not profound anaesthesia. The effect of the subsequent Bu administration was observed in the scores related to its analgesic effect. There were no significant differences between the two doses of Bu, following either 20Me-Mi or 40Me-Mi in the duration of anaesthesia, heart and respiratory rates, rectal temperature, and anaesthetic and analgesic scores except for palpebral reflex, and interdigital web clamping scores. Therefore, we concluded that the addition of 0.1 mg/kg Bu to Me-Mi elicits adequate anaesthesia with adequate analgesic effect, and side-effects such as bradycardia, hypertension, and slight respiratory acidosis in some dogs.  相似文献   

Effects of antioxidants on induction of apoptosis in bursal cells of Fabricius during in vitro cultivation     

Arai S  Itoh H  Kanda S  Endoh D  Hayashi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(1):43-47

After physically disrupting cell contacts, apoptosis of bursal cells of Fabricius was induced during in vitro cultivation. The percentage of apoptotic cells increased with incubation time and approximately 70% cells represented apoptosis after 6 hr of incubation. The induction of apoptosis was significantly inhibited by treatment of the cells with ascorbic acid (vitamin C), but not with trolox, a vitamin E analog. An intense DNA ladder pattern was shown at 6 hr post-isolation, which is a biochemical hallmark of apoptosis. Treatment of the cells with ascorbic acid inhibited the DNA fragmentation, but trolox did not. To monitor the intracellular production of reactive oxygen species (ROSs), the intensity of fluorescence emitted from DCFH-DA was measured. The intensity of fluorescence from cells incubated for 0.5-2 hr was approximately 2-fold higher than that from cells at 0 hr. The relative intensity of fluorescence decreased immediately after the addition of ascorbic acid to the cells. The intensity from the cells treated with ascorbic acid was 20-30% of that from the control cells at each incubation time. For trolox, the intensity was 50-70% of that from the control cells at each 1 to 2 hr incubation time. When ROSs-induced lipid peroxidation was assessed using cis-parinaric acid (PnA) as a monitor molecule, lipid peroxidation was found to occur in the control cells after isolation of the bursal cells. Treatment of the cells with trolox reduced lipid peroxidation, but treatment with ascorbic acid enhanced peroxidation.  相似文献   

Geographical variation in age–height relationships for dominant trees in Japanese cedar (Cryptomeria japonica D. Don) forests in Japan     

Tomohiro Nishizono  Fumiaki Kitahara  Toshiro Iehara  Yasushi Mitsuda 《Journal of Forest Research》2014,19(3):305-316

In this study, we analyzed Japanese National Forest Inventory data to investigate the geographical variation in the relationship between tree height and age for dominant trees, and the effects of climatic conditions on these relationships. Our analysis focused on Cryptomeria japonica forests in 13 regions of Japan. The age–height relationships were classified into two regional groups that were distinguished by their climatic conditions. Several categories of climatic variables (warmth, solar radiation, precipitation, and snow depth) were significantly correlated with the parameters of a model for the age–height relationships. Our results also suggest the existence of a latitudinal cline for the maximum tree height of C. japonica in Japan. In regions with cold temperatures, deep snow, low solar radiation, and low summer precipitation, C. japonica shows a late-maturity pattern for height increase, with slow initial growth and a large maximum size. In regions with the opposite climatic conditions, it shows an early-maturity pattern with fast initial growth and small maximum size.  相似文献   

Purification of swine carbonic anhydrase isoenzyme III and measurement of its levels in tissues and plasma     

T. Nishita  T. Harada  H. Sakanoue  S. Arai  S. Itoh  K. Orito  K. Arishima 《Journal of animal physiology and animal nutrition》2014,98(1):119-127

The changes in the levels of carbonic anhydrase isozyme III (CA‐III) in swine plasma and urine have not been previously determined or reported. CA‐III is relatively specific to skeletal muscles, and should therefore be a useful diagnostic marker for muscle diseases. We isolated CA‐III from swine muscle tissues and determined CA‐III levels in the plasma and urine from both healthy and diseased pigs. The levels of CA‐III in the tissues of female swine (age, 3 months) and plasma of young swine (age, 1–5 months) and adult female pigs (age, 2–3 years) were determined using the ELISA system for swine CA‐III. The mean (± SD) levels of CA‐III in the skeletal muscles were 3.8 ± 3.2 mg/g (wet tissue), and in the plasma, 230 ± 193 ng/ml at 1 month, 189 ± 208 ng/ml at 2 months, 141 ± 148 ng/ml at 3 months, 78 ± 142 ng/ml at 4 months and 53 ± 99 ng/ml at 5 months. The mean level of CA‐III in the plasma samples from 2‐ to 3‐year‐old pigs was 18 ± 60 ng/ml. CA‐III in the plasma samples was found to decrease from 1 month until 3 years of age (p < 0.01). We performed far‐western blotting to clarify the cause of the observed decrease in CA‐III in plasma. Our results demonstrated that CA‐III is bound to the transferrin and albumin. In addition, we determined that the levels of CA‐III in plasma and urine samples were higher in diseased swine compared with the healthy pigs.  相似文献   

Effect of pH-shifting on the gel forming characteristics of salt-ground meat from walleye pollack     

Fatema Hoque Shikha  Mohammed Ismail Hossain  Katsuji Morioka  Satoshi Kubota  Yoshiaki Itoh 《Fisheries Science》2006,72(4):870-876

ABSTRACT:   In order to elucidate the mechanism of the changes in gel forming characteristics of fish meat by pH-lowering, the gelation-temperature curve and the gelation-moisture content curve were examined using the acidified walleye pollack surimi or neutralized one after acidification. In the gelation-temperature curve, the gel strength was highest at 30°C and lowest at approximately 50–60°C, irrespective of pH shifting. The gel strength at 30°C and 80°C decreased with the decrease in pH value. The neutralization of acidified surimi improved the gel strength, but it was considerably lower than the original gel strength. The gel strength at 50–60°C was not affected by pH lowering. The gel strength at 80°C could not be revived to the original by pH readjustment, either in the presence or in the absence of EDTA. These results suggest that irreversible changes of meat protein take place under the low pH, and the oxidation ability of sulfhydryl (SH) groups of protein molecule is not affected by pH-shifting.  相似文献   

Effect of oral administration of a single bolus of six different protein sources on digestive physiology of red seabream Pagrus major juveniles     

Murashita  Koji  Takakuwa  Fumiaki  Matsunari  Hiroyuki  Yoshinaga  Hazuki  Yamamoto  Takeshi  Oku  Hiromi  Furuita  Hirofumi 《Fish physiology and biochemistry》2022,48(4):939-954

Fish Physiology and Biochemistry - To reveal direct effects of various protein sources on digestive physiology of red seabream, Pagrus major (38.5 ± 0.4&nbsp;g), six different...  相似文献   

A morphological study off a thyroid carcinoma in a medaka, Oryzias latipes (Temminck & Schlegel)     

T Harada  H Itoh  J Hatanaka  S Kamiya  M Enomoto 《Journal of fish diseases》1996,19(4):271-277

A large mass protruding beyond the left operculum was found in an adult male medaka, Oryzias latipes (Temminck & Schlegel). Histologically, the mass was mainly composed of thyroid follicles measuring up to 292 μm in diameter. The follicular epithelium consisted of cuboidal or squamous cells. The follicles lacked colloidal material in the lumen. The thyroid tissue infiltrated into the kidney. Electron microscopically, the tumour cells were characterized by dilation and vesiculation of rough endoplasmic reticulum, which extended throughout the cytoplasm, and an irregular outline of the nucleus. Mitotic figures and partly degenerate cells were frequently observed. Based on the above features, the tumour was diagnosed as a follicular carcinoma of the thyroid, the first reported case in the medaka.  相似文献   

Derivation of two-way volume equation for bamboo, <Emphasis Type="Italic">Phyllostachys pubescens</Emphasis>     

Hideo Suga  Akio Inoue  Fumiaki Kitahara 《Journal of Forest Research》2011,16(4):261-267

We derive a two-way volume equation for estimating the apparent culm volume of one of the largest bamboo species, Phyllostachys pubescens Mazel ex Houz. The volume equation was derived from the assumptions that (1) the relative culm form can be expressed by Kunze’s equation and (2) normal form-factors at two different relative heights are steady at constants independent of culm sizes. A total number of 200 sample culms were collected from a stand of P. pubescens in Mt. Toshima, Kumamoto Prefecture, western Japan, and were randomly bisected into two sets of data, i.e. modeling and test data. For the modeling data, normal form-factors at 0.6 and 0.9 in relative height were steady at 0.908 and 0.448, respectively. The coefficients of the volume equation were determined based on these steady values of the normal form-factors. For the test data, the apparent culm volume was estimated from diameter at breast height and culm height with the determined volume equation. The mean error and root mean square error of the estimated culm volume were, respectively, 8.120 × 10⁻⁵ and 3.291 × 10⁻³ m³, and there was no significant difference between estimated and actual volume. In conclusion, the two-way volume equation presented here appears to be convenient for predicting the apparent culm volume for P. pubescens.  相似文献   

Clinical implications of measurement of plasma atrial natriuretic peptide concentration in dogs with spontaneous heart disease     

Hori Y  Yamano S  Kanai K  Hoshi F  Itoh N  Higuchi S 《Journal of the American Veterinary Medical Association》2011,239(8):1077-1083

  相似文献