Transcriptional and proteomic analysis of endometrial and conceptus responses to cloprostenol administration in the third week of pregnancy     

Tony Hayes  Bette Anne Quinn  Brandon Lillie  Leah Schutt  Meagan Moeyaert  Rudolf Waelchli  Keith Betteridge 《Journal of Equine Veterinary Science》2012

  相似文献   

Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats     

Shuralev E  Quinn P  Doyle M  Duignan A  Kwok HF  Bezos J  Olwill SA  Gormley E  Aranaz A  Good M  Davis WC  Clarke J  Whelan C 《Veterinary microbiology》2012,154(3-4):292-297

A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected 57/60 SICTT (95.0%) positive animals in one M. bovis infected herd and 120/120 (100%) in a second herd. In a separate experiment, 28 M. caprae culture confirmed infected goats from Spain were assayed, of which 24 (85.7%) were found positive in the test. The results show that inclusion of an antibody based assay can improve the ability to identify M. bovis and M. caprae infected goats. With further development and validation the multiplex assay may prove to be a useful tool for control of M. bovis and M. caprae infection in goats.  相似文献   

Methicillin-resistant Staphylococcus aureus isolated from a veterinary surgeon and five dogs in one practice     

Leonard FC  Abbott Y  Rossney A  Quinn PJ  O'Mahony R  Markey BK 《The Veterinary record》2006,158(5):155-159

Methicillin-resistant Staphylococcus aureus (MRSA) was isolated from five dogs with wound discharges after surgical procedures at a veterinary practice, and MRSA with similar molecular and phenotypic characteristics was isolated from the nares of one veterinary surgeon in the practice. The pulsed-field gel electrophoresis patterns of all the isolates were indistinguishable from each other and from the most common human isolates of MRSA in Ireland.  相似文献   

Experimental paratuberculosis in sheep after oral, intratracheal, or intravenous inoculation: histochemical localization of dehydrogenase activities   总被引：1，自引：0，他引：1  

R S Merkal  W S Monlux  J P Kluge  A B Larsen  K E Kopecky  L Y Quinn  R P Lehmann 《American journal of veterinary research》1968,29(5):971-982

  相似文献   

Activation-induced mobilization of secretory vesicles in bovine neutrophils.     

S D Swain  D W Siemsen  A J Hanson  M T Quinn 《American journal of veterinary research》2001,62(11):1776-1781

OBJECTIVE: To characterize mobilization of secretory granules in bovine neutrophils. SAMPLE POPULATION: Neutrophils obtained from four 6- to 18-month-old Holstein cattle. PROCEDURE: Mobilization of secretory granules in bovine neutrophils was determined by measuring changes in cell-surface alkaline phosphatase activity on cells treated with various inflammatory mediators. Subcellular distribution of the alkaline phosphatase activity was determined by analysis of bovine neutrophil homogenates fractionated on density gradients. RESULTS: Alkaline phosphatase-containing secretory granules of bovine neutrophils were readily mobilized by a number of inflammatory agents, including platelet-activating factor, interleukin-8, tumor necrosis factor-alpha, lipopolysaccharide, leukotriene B4, and zymosan-activated plasma. In contrast, N-formyl-methionyl-leucyl-phenylalanine did not have a significant effect. Phorbol myristate acetate induced a biphasic response with up-regulation of cell-surface alkaline phosphatase at low doses and a return to baseline or even a reduction in cell-surface alkaline phosphatase at higher doses (> or = 10 ng/ml). Subcellular fractionation of bovine neutrophil homogenates revealed that alkaline phosphatase activity resided in light-density membrane vesicles (ie, location of secretory granules), which were distinct from specific, azurophil, and large granules. CONCLUSIONS AND CLINICAL RELEVANCE: Bovine neutrophils respond to various inflammatory mediators by mobilizing alkaline phosphatase-containing secretory granules. This suggests that the process is an important early step in the host-defense response of bovine neutrophils.  相似文献   

The elimination of injected superoxide dismutase from synovial fluid of the horse     

DE AUER  JC NG  J. HRDLICKA  AA SEAWRIGHT 《Australian veterinary journal》1989,66(4):117-119

  相似文献   

Allergic skin reactions in the horse: response to intradermal challenge with fractionated Culicoides     

A N Morrow  P J Quinn  K P Baker 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1986,33(7):508-517

  相似文献   

Assessment of copper and zinc status of farm horses and training thoroughbreds in south-east Queensland   总被引：2，自引：0，他引：2  

DE AUER  JC NG  AA SEAWRIGHT 《Australian veterinary journal》1988,65(10):317-320

The copper and zinc concentrations in the blood of stabled thoroughbred horses and in Australian Stock Horses mares at pasture, either late pregnant or lactating were determined by an atomic absorption spectroscopic method. The plasma concentration of the trace elements in these apparently normal horses were generally below the "normal" range. The plasma copper, caeruloplasmin copper, whole blood copper and plasma zinc concentrations in the stabled thoroughbreds were 0.76 +/- 0.19 micrograms/ml (n = 82), 0.56 +/- 0.14 micrograms/ml (n = 83), 0.75 +/- 0.18 micrograms/ml (n = 82) and 0.47 +/- 0.09 micrograms/ml (n = 83) respectively. The plasma copper and zinc concentrations of all the brood mares at pasture (pregnant and lactating) were 0.56 +/- 0.20 micrograms/ml and 0.47 +/- 0.11 micrograms/ml (n = 30). The plasma copper concentration of the pregnant group of mares (0.64 +/- 0.18 micrograms/ml; (n = 14) was greater than that of the lactating mares (0.49 +/- 0.21; (n = 16). Variation in the plasma copper concentration was also identified between stabled and farm horses, between horses of different stables and between horses of different ages. The proportion of plasma copper bound to caeruloplasmin was 73 +/- 11.8%. These low concentrations of copper and zinc in the plasma of apparently normal horses are of clinical significance since recent evidence has indicated that copper deficiency appears to promote the development of skeletal abnormalities in foals. An alternative to the use of a single plasma sample to identify the copper or zinc deficient horse was discussed.  相似文献   

Microscopic Growth Mechanisms for Carbon Nanotubes     

JC Charlier  De Vita A  X Blase  R Car 《Science (New York, N.Y.)》1997,275(5300):646-649

The uncatalyzed edge growth of carbon nanotubes was investigated by first-principles molecular dynamics simulations. At experimental temperatures the open end of single-walled nanotubes closed spontaneously into a graphitic dome, which may explain why these nanotubes do not grow in the absence of transition metal catalysts. On the other hand, chemical bonding between the edges of adjacent coaxial tubes ("lip-lip" interactions) trapped the end of a double-walled nanotube in a metastable energy minimum, thus preventing dome closure. These calculations show that this end geometry exhibits a high degree of chemical activity and easily accommodates incoming carbon fragments, supporting a model of growth by chemisorption from the vapor phase.  相似文献   

The effect of root-absorbed PSII inhibitors on Kautsky curve parameters in sugar beet   总被引：2，自引：0，他引：2  

M ABBASPOOR  HB TEICHER†  & JC STREIBIG 《Weed Research》2006,46(3):226-235

The effects of the photosystem II inhibitors metamitron and terbuthylazine on the shape of the Kautsky (chlorophyll fluorescence induction) curve were investigated in sugar beet grown in hydroponic culture. The objective of the study was to trace recovery processes following herbicide injury using Kautsky curve parameters. Metamitron is used for selective weed control in sugar beet because it is metabolized in this crop. In contrast, terbuthylazine is toxic to sugar beet. Two hours after treatment, various fluorescence induction curve parameters, such as maximum quantum efficiency (F_V/F_m), the relative changes at the J step (F_vj) and area (the area between the Kautsky curve and maximum fluorescence, F_m), were affected by metamitron at concentration ranges of 70–280 mg active ingredient (a.i.) L^?1 in plants treated at the four‐true‐leaf stage. Shortly after herbicide application, F_v/F_m was more affected by the hydrophilic metamitron [log(K_ow) = 0.83] than by the lipophilic terbuthylazine [log(K_ow) = 3.21], but these differences between compounds were alleviated as metamitron was metabolized and terbuthylazine was not. Terbuthylazine at 1 mg a.i. L^?1 affected sugar beet at the four‐ and six‐true‐leaf stages to the same extent, whereas metamitron at a dose of 140 mg a.i. L^?1 affected much more at four‐ than at the six‐true‐leaf stage. Sugar beet recovered from metamitron injury even at high doses (140 and 280 mg a.i. L^?1). Fluorescence induction curve parameters were similarly affected by terbuthylazine and, although sugar beet recovered from terbuthylazine injury at low doses (<0.2 mg a.i. L^?1), the Kautsky curve was irreversibly affected at higher doses (1–10 mg a.i. L^?1), leading finally to plant death. Older plants were affected later, and recovered sooner, from both herbicides.  相似文献