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The study aimed to (1) define the proportion of dogs with immune-mediated haemolytic anaemia (IMHA) that have associative and non-associative disease and (2) evaluate the utility of screening diagnostic tests in identifying potential triggers of associative IMHA. Medical records of 78 dogs diagnosed with IMHA at a specialist hospital in Sydney from July 2008 to August 2017 were reviewed. The original diagnosis was revised according to published guidelines (Garden et al., 2019) as either diagnostic, supportive or suspicious for IMHA. Associative IMHA was confirmed if immunosuppressive therapy was discontinued within six weeks of effective treatment of a potential trigger. Associative IMHA was considered possible when a potential trigger was identified but its significance could not be confirmed. Associative IMHA was confirmed (3) or suspected (7) in 10 dogs (13%, confidence interval [CI] 7.1%–22%), with 68 cases presumed to be non-associative. Associative IMHA was present in 3/29 (10.3%) of dogs with criteria diagnostic for IMHA, 4/42 (9.5%) of dogs with criteria supportive for IMHA and 3/7 (42.9%) of dogs with criteria suspicious for IMHA. Abdominal ultrasound was performed in 68 dogs and identified possible triggers in five (7.3%, CI 3.2% to 16%). Thoracic radiographs were performed in 70 dogs but did not identify any potential triggers (0%, CI 0% to 5.2%). Urine culture was performed in 22 dogs and was positive in three (14%, CI 4.7% to 33.3%). Routine screening tests, particularly thoracic radiographs, have a low yield in identifying potential triggers of associative IMHA, but are more likely to be useful in dogs fulfilling less stringent diagnostic criteria of IMHA.  相似文献   
73.
A very small amount of applied zinc (Zn) is taken up by crops, resulting in low recovery by plants. Adding elemental sulphur to zinc oxide (ZnO) fertiliser could improve Zn solubilisation and exert a higher residual effect on crops than soluble Zn sources. We produced an isotopically labelled Zn-elemental sulphur fertiliser and evaluated its performance in comparison to traditional Zn sources during sequential crop cultivation. Three 67Zn-labelled fertilisers, ZnO, zinc sulphate (ZnSO4), and ZnO co-granulated with elemental sulphur (ZnOS0), were soil applied, and their contributions to the uptake of Zn by three consecutive crops, wheat, ryegrass, and corn, were assessed in a 294-d pot experiment. The contributions of Zn fertilisers followed the order:ZnSO4 > ZnO=ZnOS0. The relative contributions of Zn fertilisers were lower in the first crop than in the subsequent crops. The overall recovery of applied Zn by the three crops was higher for ZnSO4 than for ZnO and ZnOS0, reaching 1.56%, 0.45%, and 0.33% of the applied Zn, respectively. Zinc recovery by plants was very low, regardless of the source of Zn. Adding elemental sulphur to ZnO did not increase its effectiveness up to 294 d after application. Fertiliser contribution was higher for the subsequent crops than for the initial crop, indicating the importance of assessing the residual effects of Zn fertilisers.  相似文献   
74.
Studies evaluating the effects of dobutamine in horses do not consistently report increases in cardiac output despite increases in arterial blood pressure. The concurrent administration of the α2 agonist clonidine, in people, inhibited the chronotropic effects of dobutamine and increased left ventricular stroke work ( Zimpfer et al. 1982 ). Our study was performed to determine if pre‐medication with an α2 agonist affects the response to dobutamine in anaesthetized horses. Eleven horses were anaesthetized on four separate occasions for one of four randomly assigned treatments; (I) no xylazine, no dobutamine (II) xylazine, no dobutamine (III) no xylazine, dobutamine, and (IV) xylazine, dobutamine. Horses received 0.02 mg kg?1 of butorphanol IV 10 minutes prior to anesthetic induction. Two minutes prior to induction, groups II and IV received 0.5 mg kg?1 of IV xylazine. Anaesthesia was induced with 6–7 mg kg?1 of thiopental and maintained with halothane. End‐tidal halothane concentrations were maintained between 1.1 and 1.2% in groups I and III, and 0.9–1.0% for groups II and IV. Heart rate, cardiac output, right atrial pressure, and systolic (SAP), diastolic (DAP) and mean (MAP) arterial pressure were recorded 30 minutes after beginning halothane anaesthesia (T10). Cardiac output was estimated using Lithium dilution ( Linton et al. 2000 ). Baseline measurements were repeated twice, at 5‐minute intervals (T5 and T0). At time 0 (T0), an IV infusion of either saline (100 mL hour?1) or dobutamine (0.001 mg kg?1 minute?1) was started and data recorded at 5‐minute intervals for 30 minutes (T5 – T30). Stroke volume and systemic vascular resistance (SVR) were calculated. Data were analysed using repeated measures anova (p < 0.01 significant) and Newman–Keuls for multiple comparisons. Cardiac output and stroke volume increased over time in groups III and IV. Cardiac index was higher in groups III and IV than in groups I and II from T10 until completion of the study. Estimates of cardiac index at T30 for groups I–IV were 45 ± 9, 46 ± 11, 71 ± 11, and 78 ± 19 mL kg?1 minute?1, respectively (mean ± SD). Stroke index was higher in groups III and IV than in groups I and II from T15 to T30. Values for stroke index at T30 for groups I–IV were 0.98 ± 0.19, 1.11 ± 0.18, 1.46 ± 0.21, 1.74 ± 0.33 mL kg?1. Heart rate decreased from T10–T30 in groups I and II. Heart rate was greater in groups I and III than in groups II and IV at T5 and T0. Values for heart rate at T0 for groups I–IV were 48 ± 5, 42 ± 5, 50 ± 4, 43 ± 4 beats minute?1. Systolic arterial pressure, DAP and MAP were higher in groups III and IV than in groups I and II from T5 to T30. There were no differences in SVR between groups. Dobutamine at 0.001 mg kg?1 minute?1 increased cardiac output, blood pressure, and stroke volume. Premedication with xylazine at 0.5 mg kg?1 did not appear to affect the response to dobutamine.  相似文献   
75.
Grodon首先于1980年报道,转移克隆化的外源DNA到小鼠体中,而使小鼠的所有细胞都含有外源基因。随后又有很多类似报道,被整合的外源基因,即转基因被稳定地整合到转基因动物的基因组中,并按孟德尔方式遗传。然而,转基因动物内在的潜力却首先是由Palmiter等(1982)证明的。他们发现,当转移到小鼠体内的生长激素基因表达时,明显增加了生长速度。近十年来,转基因小鼠通常都用做研究工具,很多实验室试图应用这一技术来改变家畜的基因型。到本世纪末或下世纪  相似文献   
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SUMMARY Mice trapped on farms in south-eastern Queensland had chronic abscessating osteoarthritides mainly involving the carpi and tarsi. Pleomorphic bacteria were shown by silver staining to be plentiful within lesions. Streptobacillus moniliformis was isolated from the joints, and streptobacillary polyarthritis was diagnosed. Other lesions observed included subcutaneous and hepatic abscesses.  相似文献   
78.
Resection of a fibromuscular subaortic stenosis in a dog   总被引:1,自引:0,他引:1  
The surgical correction of a case of fibromuscular subaortic stenosis in an Alsatian dog is described.  相似文献   
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