Comparison of synovial fluid culture and 16S rRNA PCR in dogs with suspected septic arthritis          下载免费PDF全文

VF Scharf  DD Lewis  JF Wellehan  HL Wamsley  R Richardson 《Australian veterinary journal》2015,93(6):204-207

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Dopamine receptors in equine ovarian tissues     

King SS  Campbell AG  Dille EA  Roser JF  Murphy LL  Jones KL 《Domestic animal endocrinology》2005,28(4):405-415

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Expression of lactoferrin in the boar epididymis: effects of reduced estrogen     

Pearl CA  Roser JF 《Domestic animal endocrinology》2008,34(2):153-159

Lactoferrin is regulated by estrogen in the female reproductive tract and evidence in immature mice suggests that it may be estrogen regulated in males as well. The estrogen regulation of lactoferrin in the epididymis of the boar, a high estrogen-producing male, is unknown. This study was designed to test the hypothesis that lactoferrin expression in the boar epididymis is regulated by estrogen. Twenty-one littermate pairs of boars were treated with vehicle or Letrozole, an aromatase inhibitor, from 1 week of age until castration at 2 through 8 months. Epididymal tissue was collected at castration and fixed for immunolocalization of lactoferrin. Epididymal and testicular tissues were also collected from five mature boars (1-2.5 years) and fixed for immunocytochemistry (ICC). Lactoferrin was localized in the principal cell cytoplasm of the caput, corpus and cauda of developing boars but only in the corpus and cauda of mature boars. Basal cells were negative for lactoferrin. Sperm in the corpus and cauda was also positive for lactoferrin. The efferent ducts and testes were negative for lactoferrin. Intensity of lactoferrin immunostaining increased with age in the corpus and cauda regardless of treatment. Reduced endogenous estrogen in the epididymis during development did not affect the intensity of immunostaining between control and Letrozole-treated animals. Lactoferrin expression in the epididymis of the developing boar does not appear to be regulated by estrogen.  相似文献   

Hormone Profiles of Mares Affected by the Mare Reproductive Loss Syndrome     

D Volkmann  W Zent  T Little  T Riddle  J Durenbereger  K Potenza  L Sibley  J Roser 《Reproduction in domestic animals》2008,43(5):578-583

While searching for the cause of the Mare Reproductive Loss syndrome (MRLS), we postulated that 1 of 3 tissues in 40–120 D pregnant mares was the likely primary target of the noxious factor that caused early abortions: The corpora lutea (CL), the endometrium or the fetus and/or its membranes. At this stage of gestation, progesterone (P4) is solely produced by luteal tissue, eCG by endometrial cups in the endometrium and oestrogens by the feto‐placental unit. We determined whether concentrations of P4, eCG and/or total conjugated oestrogens (CE) would indicate which tissue was targeted during the MRLS. P4, eCG and CE were measured in single serum samples collected from 216 mares, 60–110 D after ovulation during the 2001 MRLS outbreak. All mares had previously been confirmed pregnant by ultrasonography. The following data was obtained from each mare: Interval from ovulation, pregnancy status and normalcy of fetal fluids at the time of sampling, and pregnancy status 3 weeks after sampling and at term. There were no meaningful differences in hormone concentrations between pregnant mares that had normal and excessively echogenic fetal fluids at the time of sampling. CE were lower (p < 0.05) in mares that aborted after sample collection than in mares the carried to term. In 8 mares from which multiple samples were obtained, CE consistently decreased prior to any decreases in P4 or eCG. Arguments are presented that lead to the hypothesis that the fetal trophoblast was the primary target of the MRLS agent.  相似文献   

Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cryopreservation of European eel sperm.     

DL Garzón  DS Peñaranda  L Pérez  F Marco‐Jiménez  X Espert  T Müller  M Jover  JF Asturiano 《Reproduction in domestic animals》2008,43(1):99-105

The main objective of the present study was to evaluate the influence of pH and bicarbonate concentration in the activation or inhibition of European eel (Anguilla anguilla) spermatozoa and to evaluate the effect of different cryoprotectants: dimethyl sulphoxide (DMSO), acetamide, ethylene glycol, propanol, glycerol and methanol (MeOH). The effect of these factors was evaluated comparing the percentage of motile cells, the percentage of alive cells (by Hoechst staining) and the spermatozoa morphometry pre- and post-cryopreservation (by computer-assisted morphology analysis). Based on the above findings, three cryoprotectants (DMSO, MeOH and glycerol) were chosen and evaluated in two media (P1 and P1 modified) with different concentrations of NaHCO(3) and in the presence or absence of foetal bovine serum (FBS). The effect of these factors was evaluated comparing the percentage of alive and motile cells post-cryopreservation. DMSO was the cryoprotectant showing better results in relation to the percentage of spermatic alive cells post-freezing and caused a smaller modification of the head spermatozoa morphology. The combination of P1-modified medium with DMSO and containing FBS increased slightly but significantly the percentage of motile spermatozoa post-cryopreservation.  相似文献   

Corneal and anterior segment foreign body trauma in dogs: a review of 218 cases          下载免费PDF全文

Roser Tetas Pont  Marian Matas Riera  Richard Newton  David Donaldson 《Veterinary ophthalmology》2016,19(5):386-397

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Quantitative and thematic analysis of complaints by clients against clinical veterinary practitioners in New Zealand     

SJG Gordon  DH Gardner  JF Weston  CF Bolwell  J Benschop  TJ Parkinson 《New Zealand veterinary journal》2019,67(3):117-125

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First Production of Larvae Using Cryopreserved Sperm: Effects of Preservation Temperature and Cryopreservation on European Eel Sperm Fertilization Capacity          下载免费PDF全文

JF Asturiano  SR Sørensen  L Pérez  P Lauesen  J Tomkiewicz 《Reproduction in domestic animals》2016,51(4):485-491

Sperm cryopreservation is a useful tool in captive fish reproduction management, that is to synchronize gamete production, especially in the case of species as the European eel, where the time of female spawning readiness is unpredictable. Several protocols to cryopreserve sperm of this species have been described, but until recently fertilization trials were not feasible. This study evaluated the effect of cold storage of diluted sperm prior to fertilizations and tested whether a previously defined protocol for European eel sperm cryopreservation can be successfully applied in fertilization trials to produce viable offspring. In our experiment, the sperm motility was evaluated after the extraction and the best samples were selected and pooled. Until stripping of eggs and fertilization, diluted sperm samples were maintained at either 4 or 20°C, or cryopreserved, following existing protocols. Fertilization of two egg batches was attempted. Diluted sperm caused a similar percentage of fertilized eggs and a similar number of embryos and larvae, independently of storage temperature (4 or 20°C). The cryopreserved sperm resulted in a lower percentage of fertilized eggs, but embryos developed and a few larvae (‘cryolarvae’) were obtained 55 h after fertilization in one of the two egg batches. This result evidences that the tested cryopreservation protocol is applicable for eel reproduction management, although improvements will be required to enhance fertilization success.  相似文献   

Changes in Testicular Interstitial Connective Tissue of Hamsters (Mesocricetus auratus) During Ageing and After Exposure to Short Photoperiod          下载免费PDF全文

E Beltrán‐Frutos  V Seco‐Rovira  C Ferrer  J Martínez‐Hernández  JF Madrid  FJ Sáez  M Canteras  LM Pastor 《Reproduction in domestic animals》2016,51(1):47-53

The testicular interstitium of Syrian hamster (Mesocricetus auratus) was studied during ageing and in testicular regression after exposure to a short photoperiod, in relation to the interstitial cells and their connective tissue. This tissue was assessed histochemically using Masson's trichrome technique and the expression of Heat Shock Protein 47 (HSP‐47) and collagen IV (α5) was assessed in Leydig cells. Finally, an ultrastructural analysis of some cells of the testicular interstitium was made. Leydig cells were positive for HSP‐47 and collagen IV (α5). Ageing did not change the parameters studied while the short photoperiod altered the synthetic activity of Leydig cells. The positivity index of these cells for HSP‐47 was significantly higher in the regressed testis, but was lower for collagen IV (α5). During ageing no change were observed. Ultrastructural Leydig cells showed a discontinuous basal lamina that did not change during ageing. The basal lamina was not identified in Leydig cells regressed by exposure to a short photoperiod. In conclusion; the intertubular connective tissue suffers little change with age. By contrast, in the testis regressed after exposure to a short photoperiod the studied parameters related to the intertubular connective tissue were altered. These changes are probably related with the low synthetic activity of regressed Leydig cell.  相似文献   

Ethidium bromide: A general purpose fluorescent stain for nucleic acid in bacteria and eucaryotes and its use in microbial ecology studies     

David J. Roser 《Soil biology & biochemistry》1980,12(4):329-336

The fluorochrome ethidium bromide (EB) combines with double-stranded DNA and fluoresces when excited by u.v. radiation.When applied at aqueous concentrations ranging from 2 mg l^?1 to Ig l^?1 EB strongly stained all eucaryotic and procaryotic cell types tested. It concentrated preferentially in procaryotic cells containing DNA and in the nucleus and other cytoplasmic components of eucaryotic cells. It did not stain cell walls or inert materials, e.g. clay and sand, as intensely as material containing nucleic acid.Potential applications for EB as a stain include the detection of plant root nuclei and the examination of bacteria and fungi in soil and mud preparations.  相似文献