Mesohabitat associations of the threatened San Marcos salamander (Eurycea nana) across its geographic range          下载免费PDF全文

Peter H. Diaz  Joe N. Fries  Timothy H. Bonner  Mara L. Alexander  Weston H. Nowlin 《水产资源保护:海洋与淡水生态系统》2015,25(3):307-321

European Eel Sperm Diluent for Short‐term Storage     

DS Peñaranda  L Pérez  V Gallego  R Barrera  M Jover  JF Asturiano 《Reproduction in domestic animals》2010,45(3):407-415

The sperm of European eel shows a high density and the time of spermatozoa motility is very short after activation with sea water. These characteristics make difficult the sperm handling and its quality assessment. Several diluents were previously described for the Japanese eel obtaining over 3 weeks’ conservation times under refrigeration, but they rendered bad results in the European species. In the present study, several diluents were developed taking as basis the P1 medium, and using different dilution ratios (1 : 50, 1 : 100) and two pH (6.5, 8.5). The effect of the addition of bovine serum albumin (BSA, 2% w/v) was also evaluated. At 24 h, undiluted samples already showed significant lower motility and viability than sperm samples diluted in the different media. The results for diluents with pH 6.5 and 8.5 were different. Spermatozoa diluted in media at pH 6.5 cannot be activated at 24 h, while samples diluted in the diluents with pH 8.5 and added with BSA did not show significant differences with respect to the fresh sperm motility until 48 h. The viability (percentage of alive cells) did not show differences until 1 week, independent of the dilution ratio. After 1 week, the motility was approximately 30% in the media containing BSA, which presented no differences for head size of the spermatozoa (perimeter and area) until 72 h and 1 week, respectively. In conclusion, the combination of one medium having similar physico‐chemical characteristics to the seminal plasma, including pH 8.5, and supplemented with BSA can be used in different dilution ratios for the sperm’s short‐term storage, preserving its motility capacity.  相似文献   

Pregnancy‐Associated Glycoprotein and Progesterone Concentrations during Pregnancy Failure in Bedouin Goat from the Southwest of Algeria     

S Charallah  Z Amirat  J Sulon  F Khammar  JF Beckers 《Reproduction in domestic animals》2010,45(6):e231-e238

Thirteen female Bedouin goats living in arid land of Algeria Sahara desert were used in this study. These goats were pregnant but they sustained an abortion because of unidentified causes. None of the goats showed any signs of general disease. Plasma concentrations of caprine pregnancy‐associated glycoproteins (cPAGs) and progesterone (P4) were determined during pregnancy using radioimmunoassay. The cPAGs concentration was undetectable (<0.8 ng/ml) throughout the first 2 weeks of gestation. From week 3 after mating, cPAGs concentration was detectable with significant individual variations (p < 0.05) reaching a maximum secretion (436.1 ng/ml). Throughout gestation, cPAGs concentration remained relatively constant but decreased few days before abortion, on an average of 9.2 ± 1.2 days (n = 11), except for two females where the concentrations decreased later (1–2 days before abortion). One or two peaks of cPAGs concentrations (in 4/13 and in 9/13 females, respectively) have been measured few weeks before abortion (77–124 days after mating), when a decline of cPAGs was detected. The P4 concentration increased after mating, and was high from the first week till the end of pregnancy. The P4 concentration (9.1 ± 0.9 ng/ml) decreased rapidly (<0.5 ng/ml) after 4 ± 0.7 days (n = 6) or 9.4 ± 1.6 days (n = 7) before abortion. A positive relationship (p < 0.01) was found between P4 and cPAGs concentrations during gestation. Results indicate that cPAGs and P4 measurements can be used for monitoring gestation and for abortion prediction.  相似文献   

Effect of processing variables on potato granule production     

W. O. Harrington  R. L. Olson  W. J. Weston  Mary L. Belote 《American Journal of Potato Research》1959,36(7):241-254

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Abstracts of papers presented at the Forty-First annual meeting     

Jay L. Haddock  Ray D. Jackson  W. O. Harrington  R. L. Olson  W. J. Weston  Mary L. Belote  W. J. Hooker  A. P. Benson  R. W. Hougas  S. J. Poloquin  R. W. Ross  G. V. C. Houghland  Wm. G. Hoyman  D. R. Isleib  N. R. Thompson  T. Miyamoto  E. J. Wheeler  S. T. Dexter  Hugh J. Murphy  Michael Goven  Alvah L. Perry  G. H. Rieman  D. B. Robinson  R. H. Larson  J. G. Walker  R. L. Sawyer  Lawrence A. Schaal  Clark H. Livingston  Ora Smith  W. L. Smith  Vilhelm Umaerus  L. M. Ware  W. A. Johnson  R. E. Webb  E. S. Schultz  Donald A. Young  L. C. Young 《American Journal of Potato Research》1958,35(3):441-449

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Localization of Insulin‐Like Growth Factor‐I (IGF‐I) and IGF‐I Receptor (IGF‐IR) in Equine Testes     

MJ Yoon  T Berger  JF Roser 《Reproduction in domestic animals》2011,46(2):221-228

The insulin‐like growth factor‐I (IGF‐I) is a key regulator of reproductive functions. IGF‐I actions are primarily mediated by IGF‐IR. The main objective of this research was to evaluate the presence of IGF‐I and IGF‐I Receptor (IGF‐IR) in stallion testicular tissue. The hypotheses of this study were (i) IGF‐I and IGF‐IR are present in stallion testicular cells including Leydig, Sertoli, and developing germ cells, and (ii) the immunolabelling of IGF‐I and IGF‐IR varies with age. Testicular tissues from groups of 4 stallions in different developmental ages were used. Rabbit anti‐human polyclonal antibodies against IGF‐I and IGF‐IR were used as primary antibodies for immunohistochemistry and Western blot. At the pre‐pubertal and pubertal stages, IGF‐I immunolabelling was present in spermatogonia and Leydig cells. At post‐pubertal, adult and aged stages, immunolabelling of IGF‐I was observed in spermatogenic cells (spermatogonia, spermatocyte, spermatid, and spermatozoa) and Leydig cells. Immunolabelling of IGF‐IR was observed in spermatogonia and Leydig cells at the pre‐pubertal stage. The immunolabelling becomes stronger as the age of animals advance through the post‐pubertal stage. Strong immunolabelling of IGF‐IR was observed in spermatogonia and Leydig cells at post‐puberty, adult and aged stallions; and faint labelling was seen in spermatocytes at these ages. Immunolabelling of IGF‐I and IGF‐IR was not observed in Sertoli cells. In conclusion, IGF‐I is localized in equine spermatogenic and Leydig cells, and IGF‐IR is present in spermatogonia, spermatocytes and Leydig cells, suggesting that the IGF‐I may be involved in equine spermatogenesis and Leydig cell function as a paracrine/autocrine factor.  相似文献   

Serological evidence for exposure to bovine viral diarrhoea virus in sheep co-grazed with beef cattle in New Zealand     

CA Evans  J-H Han  JF Weston  C Heuer 《New Zealand veterinary journal》2020,68(4):238-241

ABSTRACT

Aims: To determine whether sheep that co-grazed with cattle that were suspected to be positive for bovine viral diarrhoea (BVD) virus had serological evidence of exposure to the virus.

Methods: Eighteen commercial farms that routinely co-grazed cattle and sheep in the same paddocks were recruited through purposive sampling. The recruiting veterinarians identified nine farms with cattle herds that were known or highly suspected to be positive for BVD and nine farms that were considered to be free of BVD. Blood samples were taken from 15 ewes aged 1 year on each farm and samples were submitted to a commercial diagnostic laboratory to test for antibodies against pestiviruses using an ELISA. All samples that were positive were then tested using a virus neutralisation test (VNT)for antibodies against BVD virus.

Results: Of the 270 blood samples, 17 were positive for pestivirus antibodies by ELISA and these originated from two farms that were known or suspected to have BVD virus-positive cattle. None of the samples from the nine flocks co-grazed with cattle herds that were known or suspected to be BVD virus-negative were positive for pestivirus antibodies. Within the two positive farms, 2/15 samples from the first farm and 15/15 samples from the second farm were antibody-positive. When the 17 positive blood samples were submitted for VNT, all 15 samples from the second farm tested positive for BVD virus antibodies with the highest titre being 1:512.

Conclusions and clinical relevance: In this small sample of New Zealand sheep and beef farms with suspected BVD infection in cattle, there was evidence of pestivirus exposure in co-grazed sheep. Although we were unable to confirm the origin of the exposure in these sheep, these findings highlight that farmers who are trying to eradicate BVD from their cattle should be mindful that the infection may also be circulating in sheep, and both populations should be considered a possible risk to each other for generating transient and persistent infections. Further work is needed to estimate the true prevalence of New Zealand sheep flocks that are affected by BVD and the associated economic impacts.  相似文献   

Differential Growth Response to Salt Stress Among Selected Ornamentals     

Seok Hyun Eom  Timothy L. Setter  Antonio DiTommaso  Leslie A. Weston 《Journal of plant nutrition》2013,36(7):1109-1126

Evaluation of salt tolerance in herbaceous perennials was performed with mature potted plants under greenhouse conditions. Six herbaceous perennial species were evaluated for their tolerance to aqueous solutions of various sodium chloride (NaCl) concentrations over a 21 day period by measuring growth, water transpiration, and leaf nutrient content. Potential exists for utilization of these species in somewhat challenging saline environments along roadsides and in urban landscapes. Species evaluated in a mature growth stage included Achemilla mollis, Nepeta x faassenii, Sedum acre, Thymus praecox, Phlox subulata and Solidago cutleri. On the basis of relative growth rate and water transpiration responses to NaCl (0–400 mM) treatments, groundcovers were grouped into three tolerance categories: highly sensitive to salt treatment (S. acre), those with intermediate sensitivity (A. mollis, N. x faassenii, T. praecox, and P. subulata), and those exhibiting tolerance (S. cutleri). Sodium content in leaf foliage of S. cutleri was about ten-fold lower than other groundcover species in the 200 mM NaCl treatment, consistent with greater tolerance to NaCl treatments in terms of transpiration, biomass accumulation, and retention of green foliage. Comparison of foliar nutrient levels among groundcover species and treatments suggested strong differential response to NaCl treatment, indicating that changes in nutrient levels over time may be a reasonable way to predict NaCl tolerance in groundcovers.  相似文献   

Genetic parameter estimates for growth and form traits in common ash (Fraxinus excelsior, L.) in a breeding seedling orchard at Little Wittenham in England   总被引：3，自引：1，他引：2  

Weston F. Mwase  Peter S. Savill  Gabriel Hemery 《New Forests》2008,36(3):225-238

Data from a trial of 36 families at Little Wittenham, North Oxford was used to assess growth traits and stem form in common ash (Fraxinus excelsior, L.). The trial followed a design described by Barnes (Silv Genet 44(2–3):81–88, 1995) as the Breeding Seedling Orchard (BSO). The objectives of the study were to determine variation in growth and form traits and estimate genetic parameters of families. Assessments conducted at 3–8 years for height, diameter at breast height (dbh), straightness and forking showed significant differences (P < 0.05) among families with Guys (19), Settrington (34), Overbury (23) and Grimthorpe (36) being the best performers while Coleford (3 and 5) Gwynedd (27) and Bacheiddon (32) were the least performers. At the overall level, the additive genetic coefficient of variation (CV_A) ranged between 10.2 and 12.7% for height and 1.5 and 2.1% for dbh. Height had a high genetic correlation (r_g = 0.85) with dbh whilst forking had low correlation (r_g = 0.25) with height. Estimates of heritability decreased with age from  = 0.62 at 3 years to  = 0.30 at 8 years for height while values for dbh decreased from  = 0.48 at 4 years to  = 0.27 at 8 years. Some genetic gains are possible despite the low genetic variation among families; height had the greatest potential of genetic gain (12.5% at 8 years). Results suggest that early selection of superior families of Fraxinus excelsior appears to be feasible and it could be possible to achieve simultaneous improvement in both growth and form.  相似文献   

Plasma luteinizing hormone and progesterone concentrations in goats with estrous cycles of normal or short duration after prostaglandin F2 alpha administration during diestrus or pregnancy     

K N Bretzlaff  P G Weston  J E Hixon  R S Ott 《American journal of veterinary research》1988,49(6):939-943

Plasma luteinizing hormone (LH) and progesterone concentrations were compared in does experiencing short-duration estrous cycles and in does with estrous cycles of normal duration. The short-duration estrous cycles were observed immediately after induction of abortion in pregnant does by use of prostaglandin (PG) F2 alpha. Intramuscular administration of 5 mg of PGF2 alpha was accomplished in 8 does that were 52 to 63 days into gestation and in 9 cycling does at 7 to 10 days after estrus. In both groups, the mean plasma concentration of progesterone decreased from a luteal phase concentration immediately before to less than 1 ng/ml by 24 hours after PGF2 alpha administration. Of the 8 does that aborted, 6 experienced short-duration estrous cycles, and 4 of these 6 had an LH surge during the time of blood sample collection. The mean time from PGF2 alpha administration to the LH surge was significantly (P less than 0.05) longer in does with short-duration estrous cycles (71 hours) than that in does with estrous cycles of normal duration (58 hours). The mean area under the LH concentration curve was significantly (P less than 0.005) less for does with short-duration estrous cycles. Short-duration estrous cycles were associated with delayed preovulatory LH surges of reduced magnitude.  相似文献