Effects of estrone infusion on secretion of F series prostaglandins and on function of the corpus luteum during late gestation in cattle     

C A Pimentel  R Vijayakumar  P G Weston  S M Pimentel  W C Wagner  J E Hixon 《American journal of veterinary research》1986,47(9):1972-1977

Catheters were surgically implanted in the carotid artery, jugular vein, and middle uterine vein of 8 cows at 245 days of gestation. Four cows were given 4 mg of estrone/hour via continuous jugular infusion from 0800 hours on day 246 of gestation through 0800 hours on day 250 of gestation; the remaining 4 cows (controls) were given the vehicle for estrone at 10 ml/hour for the same period. Blood samples were collected from the carotid artery every 3 hours during the infusion. Samples were collected hourly from the middle uterine vein from 0 through 8, 54 through 66, and 112 through 120 hours of the infusion periods. After completion of the infusion, corpora lutea were enucleated and blood samples were collected from the carotid artery and uterine vein at hourly intervals for an additional 8 hours. Dispersed cell preparations of the corpora lutea were incubated with and without luteinizing hormone (LH) or dibutyryl cyclic adenosine monophosphate (dbcAMP). Circulating concentrations of unconjugated and conjugated estrone and estradiol-17 beta were higher (P less than 0.05) in the group infused with estrone than in the vehicle-infused group. Mean and base-line concentrations of F series prostaglandins (PGF) for each blood collection period tended to increase (P less than 0.10) during infusion with estrone, but not during infusion with vehicle. After luteectomy, mean and base-line concentrations of PGF also tended (P less than 0.10) to be greater in the estrone-infused cows than in the control cows, but a surge in PGF concentrations due to removal of the ovarian source of progesterone did not develop.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Association of parainfluenza-3 virus with bovine macrophages and blood cells: an in vitro study   总被引：1，自引：0，他引：1  

E H Stauber  K J Weston 《American journal of veterinary research》1984,45(3):583-585

Bovine blood cells and peritoneal and lung macrophages were exposed in vitro to parainfluenza-3 (PI-3) virus. Residual nonadsorbed PI-3 virus (expressed in percentage of input virus) in the supernate of the various cell fractions 1 hour after incubation at 37 C was as follows: lung macrophages, 11%; peritoneal macrophages, 59%; monocytes, 26%; RBC, 14%; lymphocytes, 28%; and polymorphonuclear cells (PMN), 63%. Lung macrophages, monocytes, lymphocytes, and PMN were monitored over a 72-hour period for hemadsorption of chicken RBC. Hemadsorption increased for lung macrophages and monocytes, whereas it decreased for lymphocytes and PMN. Infective virus could not be recovered from PMN, RBC, lymphocytes, or monocytes for more than 24 hours after PI-3 infection. Recovery of infective PI-3 virus from infected peritoneal and lung macrophages extended over 4 to 8 days, respectively.  相似文献   

Concentration of Arachidonic Acid Metabolites in Arterial and Uterine Venous Blood During the Last Month of Pregnancy in Cows*     

M. Hoedemaker  P.G. Weston  W.C. Wagner 《Reproduction in domestic animals》1991,26(6):315-317

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Use of hair root as a source of DNA for the detection of heterozygotes for recessive defects in cattle     

PJ HEALY  JA DENNIS  JF MOULE 《Australian veterinary journal》1995,72(10):392-392

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Progress in identifying and maintaining salmonella-free commercial chicken breeding flocks. 1. 1967-1969   总被引：1，自引：0，他引：1  

G H Snoeyenbos  B A McKie  C F Smyser  C R Weston 《Avian diseases》1970,14(4):683-696

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Avian tuberculosis in naturally infected captive water birds of the Ardeideae and Threskiornithidae families studied by serotyping, IS901 RFLP typing, and virulence for poultry   总被引：2，自引：0，他引：2  

Dvorska L  Matlova L  Ayele WY  Fischer OA  Amemori T  Weston RT  Alvarez J  Beran V  Moravkova M  Pavlik I 《Veterinary microbiology》2007,119(2-4):366-374

Avian tuberculosis was detected in one flock of 38 water birds of the families Ardeideae (n = 20) and Threskiornithidae (n = 18). Mycobacterium avium subsp. avium (MAA, serotype 1, genotype IS901+ and IS1245+) was more often (p = 0.01) detected in tissue and/or faecal samples in 18 (90.0%) birds form the Ardeideae family: little egret (Egretta garzetta), buff-backed heron (Bubulcus ibis), great white egret (Egretta alba), and bittern (Botaurus stellaris) in comparison to two (11.1%) birds from the Threskiornithidae family: sacred ibis (Threskiornis aethiopicus). Avian tuberculosis was not diagnosed in spoonbills (Platalea leucorodia). Tuberculous lesions were found in nine birds. MAA isolates of IS901 RFLP type F-C3 were present in all of the 20 infected birds and in all environmental isolates. A mixed infection with the MAA isolates of three RFLP types F-C3 (tissue isolate), G-C3, and T-C3 (faecal isolates) was found in one sacred ibis. All 20 tissue isolates of IS901 RFLP type F-C3 from 20 birds and 8 environmental MAA isolates were fully virulent in pullets, whilst the isolates of RFLP types G-C3 and T-C3 were non-virulent in pullets. All of the tested MAA isolates had the same IS1245 RFLP "bird profile". In 12 of 20 infected birds with MAA M.a. hominissuis isolates of serotypes 4, 8, 9 and genotype IS901- and IS1245+ were detected and in 8 other birds mycobacteria not belonging to the M. avium complex were found. The presence of MAA in the environment may be a source for further spread of the causal agent of avian tuberculosis among other groups of animals in zoological gardens, farm animals, and also among their keepers.  相似文献   

Retrospective evaluation of the efficacy of isolating bacteria from synovial fluid in dogs with suspected septic arthritis          下载免费PDF全文

VF Scharf  ST Lewis  JF Wellehan  HL Wamsley  R Richardson  DA Sundstrom  DD Lewis 《Australian veterinary journal》2015,93(6):200-203

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Colour Doppler Ultrasonography as a Tool to Assess Luteal Function in Santa Inês Ewes          下载免费PDF全文

LM Figueira  JF Fonseca  EKN Arashiro  JMG Souza‐Fabjan  ACS Ribeiro  E Oba  JHM Viana  FZ Brandão 《Reproduction in domestic animals》2015,50(4):643-650

The aim of this study was to evaluate luteal dynamics in the Santa Inês ewes using colour Doppler (CD) ultrasonography. Oestrus was synchronized in nulliparous females (n = 18), and subsequently, they were only teased (n = 6) or teased and mated (n = 12). Blood samples were collected daily for plasma progesterone (P₄) concentrations. Ultrasonographic images of corpora lutea (CL) in CD mode were obtained for further analysis in its largest diameter. The CD mode allowed an early sequential monitoring of CL that was visualized by the first time 0.77 ± 0.62 days after ovulation, with luteal area 29.68 ± 13.21 mm². During the luteogenesis, a progressive increase was observed, followed by a plateau of luteal area, vascularization area and plasma concentrations of P₄ reaching maximum values in D11 (124.0 ± 38.0 mm², 52.78 ± 24.08 mm² and 11.23 ± 4.89 ng/ml, respectively). In the luteolysis, the plasma concentrations of P₄ decreased sharply, whereas luteal and vascularization area gradually. The vascularization area was positively correlated with plasma concentrations of P₄ during the luteogenesis (r = 0.22) and luteolysis (r = 0.48). The luteal dynamics of Santa Inês ewes showed patterns similar to those observed in other sheep breeds studied. The CD ultrasonography has the potential to be used as a tool to assess luteal function in sheep.  相似文献   

The equine guttural pouch: Clinically relevant anatomy and basic treatment options for trauma          下载免费PDF全文

J. Weston Davis  C.J. Caniglia 《Equine Veterinary Education》2015,27(8):403-404

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ELASTOGRAPHIC EVALUATION OF NATURALLY OCCURING TENDON AND LIGAMENT INJURIES OF THE EQUINE DISTAL LIMB          下载免费PDF全文

Meghann Lustgarten  W. Rich Redding  Raphael Labens  Weston Davis  Thomas M. Daniel  Emily Griffith  Gabriela S. Seiler 《Veterinary radiology & ultrasound》2015,56(6):670-679

Compression elastography is an ultrasonographic technique that estimates tissue strain and may have utility in diagnosing and monitoring soft tissue injuries in the equine athlete. Recently, elastography has been proven to be a feasible and repeatable imaging modality for evaluating normal tendons and ligaments of the equine distal forelimb. The purposes of this prospective study were to investigate the ability of elastography to detect spontaneously occurring lesions of equine tendons and ligaments diagnosed with magnetic resonance imaging (MRI) and gray‐scale ultrasound (US) and to characterize the differences in the elastographic appearance of acute vs. chronic injuries. Fifty seven horses with a total of 65 lesions were evaluated. Images were assessed quantitatively and qualitatively. Acute lesions were found to be significantly softer (P < 0.0001) than chronic lesions (P < 0.0001) and the stiffness of lesions increased with progression of healing (P = 0.0138). A negative correlation between lesion hypoechogenicity and softness was appreciated with more hypoechoic lesions appearing softer (P = 0.0087) and more hyperechoic regions harder (P = 0.0002). A similar finding occurred with increased signal intensity on short tau inversion recovery (STIR) and proton density (PD) MRI sequences correlating with increased softness on elastography (P = 0.0164). Using US and MRI as references, commonly encountered soft tissue injuries of the equine distal limb could be detected with elastography. However, elastography was limited for detecting small, proximal injuries of the hindlimb proximal suspensory ligament. Elastographic evaluation of equine tendons and ligaments may allow better characterization of lesion chronicity and severity, and sequential examinations may optimize lesion management, rehabilitation, and return to training.  相似文献