Expression pattern of GLUT 1, 5, 8 and citrate synthase transcripts in buffalo (Bubalus bubalis) preimplantation embryos produced in vitro and derived in vivo     

Gutulla Taru Sharma  Amar Nath  Shiv Prasad  Sumit Singhal  Vikash Chandra  Gutulla Saikumar 《Reproduction in domestic animals》2020,55(10):1362-1370

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Exogenous cholesterol prevents cryocapacitation-like changes,membrane fluidity,and enhances in vitro fertility in bubaline spermatozoa     

Jeetendra Singh Rajoriya  Jai Kishan Prasad  Snehal Shalik Ramteke  Ponraj Perumal  Arun Kumar De  Subrata Kumar Ghosh  Sadhan Bag  Archana Raje  Mahak Singh  Anuj Kumar  Arumugam Kumaresan 《Reproduction in domestic animals》2020,55(6):726-736

A study was conducted to determine the optimum dosage of the exogenous cholesterol-loaded cyclodextrins (CLC) to get maximum cryoprotection for bubaline spermatozoa. In the present study, 120 × 10⁶ spermatozoa were incubated in 2, 3 and 4 mg of CLC as grouped as Gr II, III and IV, respectively, and sperm progressive motility, intracellular Ca²⁺, capacitation status by protein tyrosine phosphorylation (PTP) assay and zona binding per cent (ZBP) and cleavage rate (CR) of the cryopreserved buffalo spermatozoa by in vitro fertility assay were assessed in comparison with an untreated control group (Gr I). Results revealed that there was a significant (p < .05) linear decrease in percentage of sperm population with higher intracellular Ca²⁺ and percentage of sperm population with medium or high capacitated by PTP in CLC treated from 2 to 3 mg and then increased to 4 mg/120 × 10⁶ spermatozoa whereas sperm progressive motility, percentage of sperm population with low capacitated, ZBP and CR were increased significantly (p < .05) in sperm population treated from 2 to 3 mg CLC and then decreased to 4 mg/120 × 10⁶ spermatozoa. The study has clearly indicated that CLC at 3 mg/120 × 10⁶ spermatozoa has maximum beneficial effects in protection of sperm progressive motility, membrane fluidity (low intracellular Ca²⁺); prevention of cryocapacitation (low capacitation pattern in immunolocalization) and enhancement of in vitro ZBP and CR. Post-thaw motility of the CLC-treated sperm has shown positively significant (p < .05) correlation with sperm population with low intracellular Ca²⁺, low capacitated sperm population, ZBP and CR, whereas it was negatively (p < .05) correlated with sperm population with high intracellular Ca²⁺, medium or high capacitated sperm. The present study has revealed for the first time that incubation of spermatozoa with CLC of higher dose (>3 mg/120 × 10⁶ spermatozoa) had adverse effects on sperm cryopreservation, although incubation of sperm with 3 mg/120 million prior to processing had minimised the freezing–thawing-associated damages in bubaline species.  相似文献   

Weed Problems in Chotanagpur Agriculture     

M. S. Subba Rao  L. K. Prasad 《国际虫害防治杂志》2013,59(3):286-289

Abstract

Micron Sprayers Ltd have produced a sprayer, the Herbi, designed to produce uniform atomisation with both oil- and water-based formulations. Three different nozzle sizes give low, medium and high flow rates. In performance tests wetting agents were found to be necessary to produce uniform droplets. The volume distribution across the swath showed four peaks and for the flow rate, liquid and atomiser height examined the two major peaks were at 50 and 60 cms either side of the line of the sprayer, indicating a peak to peak width of 110 cm. Of the collected spray, 75% settled within a central band of 120 cm, 99% within 180 cm and 100% within 195 cm. To prevent superimposition of peaks leading to high overdose, a swath width of 150–160 cm is suggested, such that overlap occurs at the outer position of half peak height. To avoid excessive spray drift it is recommended that the sprayer is not used in any but very light wind. The authors conclude that this machine should prove very useful for very low volume application between rows in bush and tree crops, and for treating larger areas.  相似文献   

ROC analysis of prepartum feeding time can accurately predict postpartum metritis development in HF crossbred cows     

Tapas Kumar Patbandha  Tushar Kumar Mohanty  Siddhartha Shankar Layek  Arumugam Kumaresan  Suresh C. Kantwa  R. Malhotra  A.P. Ruhil  Shiv Prasad 《Journal of Veterinary Behavior: Clinical Applications and Research》2013,8(5):362-366

Receiver operating characteristic (ROC) analysis is a useful statistical tool to visualize, organize, and select diagnostic variables. Accuracy of the test is represented by the area under the ROC curve (AUC), sensitivity (Se), and specificity (Sp). In the present study, ROC analysis of prepartum feeding time (day ?11 to day ?2) of 20 pluriparous Holstein-Friesian crossbred cows were recorded, and the cows were followed up for detecting postpartum metritis development if any. Prepartum daily feeding time data during whole study was grouped into 2 periods (P1 [day ?11 to day ?7] and P2 [day ?6 to day ?2]). The AUC of feeding time for the P1 and P2 periods was 0.64 (P = 0.29) and 0.86 (P = 0.006), respectively. Cows with daily feeding times below the optimum threshold value of 284.5 min/d during P2 (Se = 75% and Sp = 91.67%) had more chance of developing postpartum metritis compared with those with daily feeding time above the threshold value (positive likelihood ratio = 9.0). Furthermore, relative risk (RR) analysis revealed that the risk of development of postpartum metritis increased as feeding time decreased below the optimum threshold value during P2 (RR = 5.571, P = 0.004). Thus, the optimum threshold value of feeding time during P2 may be used to predict risk of postpartum metritis, allowing for preventive measures to be implemented in the early postpartum period to reduce the likelihood of postpartum complications.  相似文献   

Age-dependent patterns of bovine tuberculosis in cattle     

Ellen Brooks-Pollock  Andrew JK Conlan  Andy P Mitchell  Ruth Blackwell  Trevelyan J McKinley  James LN Wood 《Veterinary research》2013,44(1):97

Bovine tuberculosis (BTB) is an important livestock disease, seriously impacting cattle industries in both industrialised and pre-industrialised countries. Like TB in other mammals, infection is life long and, if undiagnosed, may progress to disease years after exposure. The risk of disease in humans is highly age-dependent, however in cattle, age-dependent risks have yet to be quantified, largely due to insufficient data and limited diagnostics. Here, we estimate age-specific reactor rates in Great Britain by combining herd-level testing data with spatial movement data from the Cattle Tracing System (CTS). Using a catalytic model, we find strong age dependencies in infection risk and that the probability of detecting infection increases with age. Between 2004 and 2009, infection incidence in cattle fluctuated around 1%. Age-specific incidence increased monotonically until 24–36 months, with cattle aged between 12 and 36 months experiencing the highest rates of infection. Beef and dairy cattle under 24 months experienced similar infection risks, however major differences occurred in older ages. The average reproductive number in cattle was greater than 1 for the years 2004–2009. These methods reveal a consistent pattern of BTB rates with age, across different population structures and testing patterns. The results provide practical insights into BTB epidemiology and control, suggesting that targeting a mass control programme at cattle between 12 and 36 months could be beneficial.  相似文献   

A survey of owners' perceptions of fear of fireworks in a sample of dogs and cats in New Zealand     

AR Dale  JK Walker  MJ Farnworth  SV Morrissey  NK Waran 《New Zealand veterinary journal》2013,61(6):286-291

Abstract

AIMS: To establish reliable information regarding the behavioural responses of dogs and cats to fireworks in New Zealand; record interventions used by owners, and their perceived efficacies; and establish the prevalence of firework-related injury, and quantify owners' attitudes towards fireworks.

METHODS: A questionnaire targeting dog and cat owners was distributed via the Auckland Society for the Prevention of Cruelty to Animals (SPCA) Animals Voice magazine and 25 veterinary clinics. The questionnaire covered demographics of animals, fear of fireworks, severity of the fear, and behaviours exhibited. Also included were treatments tried, source and perceived efficacy, prevalence of injury, and owners' attitudes towards the sale of fireworks for private use.

RESULTS: From a total of 8,966 questionnaires distributed, 1,007 valid questionnaires were returned, representing 3,527 animals. Of these 1,635 (46%) animals displayed a level of fear of fireworks recognisable to their owners. Owners of dogs identified a significantly higher fear response than owners of cats but the duration of these fear responses did not differ between species. Fear of fireworks frequently resulted in dogs exhibiting active fear behaviours, whereas cats were more likely to exhibit hiding and cowering behaviours. A significantly increased severity and duration of fear response over time in dogs and cats was associated with owners who comforted them when they displayed a fearful response. Only 141/890 (15.8%) of owners sought professional treatment from a veterinarian, animal behaviourist or animal trainer for their animals, with variable efficacy. Six percent (51/923) of animals had received physical injuries from fireworks. The majority (837/1,007; 83%) of respondents, regardless of whether they owned a fearful animal or not, supported a ban on the sale of fireworks for private use.

CONCLUSIONS: The results provide valuable information that is, as yet, unsubstantiated in New Zealand, although potential biases exist due to the non-random selection of respondents. Differences between dogs and cats were likely due to differing responses to fear-provoking stimuli between the species. Owner-reported increase in fearful response over time for comforted animals may indicate a negative impact on the longer-term psychological welfare of their animal.

CLINICAL RELEVANCE: The greater the awareness of effective treatment plans for animals that suffer from a fear of fireworks, the greater the possibility that this fear can be reduced. Wider dissemination of effective owner behaviour and treatment programmes for firework fears is needed to improve levels of professional treatment for dogs and cats.  相似文献   

Evaluation of efficiency of hemi-nested PCR assay for the detection of ‘Candidatus Liberibacter’ infecting citrus     

Bhaskara  Adpangaya Sahana  Ahmed  Bandalli N. Jawad  Adkar-Purushothama  Charith R.  Prasad  Mysore N. Nagendra  Sreenivasa  Marikunte Y.  Maheshwar  Pavagada K. Murthy 《植物病害和植物保护杂志》2013,120(5-6):189-193

Journal of Plant Diseases and Protection - The present study reports the development and evaluation of a hemi-nested polymerase chain reaction (hnPCR) assay for the efficient detection of...  相似文献   

Growth performance and rumen development in Malabari kids reared under different production systems     

Kotresh Prasad  C.  Abraham  John  Panchbhai  Girish  Barman  Deepandita  Nag  Pradeep  Ajithakumar  H. M. 《Tropical animal health and production》2019,51(1):119-129

Tropical Animal Health and Production - In order to investigate the effect of rearing systems on growth and rumen development in Malabari male kids, 14 pre-weaned Malabari male kids of uniform...  相似文献   

Salinity tolerance of Rhizobium mutants: Growth and relative efficiency of symbiotic nitrogen fixation     

Raman Rai  V. Prasad 《Soil biology & biochemistry》1983,15(2):217-219

A salinity-tolerant strains of Rhizohium able to grow and fix nitrogen in symbiosis with lentil (Lens esculenta) in saline soil was derived frorn effective Rhizobium strain RL 5. A forced mutation with the mutagen nitrosoguanidine resulted in the isolation of five different mutant strains. The salinity tolerance, streptomycin resistance, growth, nodulation behaviour and relative efficiency of symbiotic N₂-fixation of these strains were studied. Among the five mutants and parent, LM 4 and LM 1 successfully tolerated 200 μ g ml^?1 streptomycin and 1.5%NaCl. These two mutants also significantly increased number and dry weight of nodules per plant, dry matter yield of the crop and N₂-fixation. Between the two, LM 4 seemed generally the better.  相似文献   

Morphology and composition of some soils under cultivation in Saudi Arabia     

I.I. Bashour  A.S. Al-Mashhady  J. Devi Prasad  T. Miller  M. Mazroa 《Geoderma》1983,29(4):327-340

Twelve profiles representing the principal soils of five major agricultural areas (Al-Kharj, Gassim, Hofuf, Taif and Wadi Dawasir) in Saudi Arabia were described and sampled by horizons. Laboratory analyses were then made of the samples to characterize the profiles more completely. Analyses were also made for certain nutrient elements to get some measure of their levels in the soils.The profiles are tentatively classified in the Entisol and Aridisol orders of the American taxonomy. Five Entisol profiles were considered to be Fluvents and two Psamments. Four Aridisol profiles were considered to be Argids and one an Orthid.All of the profiles are saline and calcareous and most are also sandy. Furthermore, amounts of nutrient elements such as phosphorus, iron and zinc in surface layers are below minimum levels established for similar soils in earlier investigations in other countries. Consequently, the soils are believed to lack sufficient quantities of several elements for optimum plant growth. Successful use of the soils will thus require reductions in salinity, increased levels of nutrient elements, and skillful irrigation to provide adequate moisture.  相似文献