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71.
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JL O'RIELLY 《Australian veterinary journal》1993,70(12):442-444
SUMMARY: The IgG concentration of plasma from 13 mares was measured by radial immunodiffusion when fresh and after storage at -4°C and thawing by 3 methods. There was no significant (P > 0.05) reduction in the IgG concentration when plasma was thawed over 6 hours at 22°C (1352.9 ± 101.6 mg/dL) (mean ± SEM) compared with the fresh sample (1369.5 ± 88.1 mg/dL). In contrast, there was a significant (P < 0.05) reduction in the IgG concentration of plasma that was rapidly thawed at 57°C over 50 minutes (1142.9 ±66.2 mg/dL), or placed in a microwave oven for 20 to 25 minutes (1093.4 ± 74.4 mg/dL). When plasma is administered to hypo-gammaglobulinaemic newborn foals, a larger volume is required when thawing is rapid than when thawing is slow or when fresh plasma is administered. 相似文献
73.
W F Jarrett B W O'Neil J M Gaukroger H M Laird K T Smith M S Campo 《The Veterinary record》1990,126(18):449-452
Calves were vaccinated with two preparations made from one cutaneous fibropapilloma induced by bovine papillomavirus type 2 (BPV-2). One vaccine consisted of homogenised tumour; the other contained purified virus only. Both produced resistance to a heavy challenge infection of BPV-2. One calf in the vaccinated group developed a small tumour and rejected it earlier than the control calves. It would appear likely that the prophylactic immune response was induced by viral structural proteins only and that tumour-specific antigens are unnecessary. Bovine fibroblasts were transformed in vitro by BPV-2 and administered as a vaccine; immunity was not induced. 相似文献
74.
Procedure for successful interspecific embryo transfer from mouflon (Ovis gmelini musimon) to Spanish Merino sheep (Ovis aries). 总被引:1,自引:0,他引:1
J Santiago-Moreno A González-Bulnes A Gómez-Brunet M J Cocero A del Campo R García-García A López-Sebastián 《Journal of zoo and wildlife medicine》2001,32(3):336-341
Embryos from five anesthetized mouflons (Ovis gmelini musimon), superovulated with FSH-o (Ovagen) were transferred into preselected Spanish Merino sheep (Ovis aries). Myorelaxation was complete in four of five donor mouflons. The status of the uterus of potential recipients was evaluated by transrectal ultrasonography, and those ewes with fluid in the uterine horn were rejected. The corpus luteum in each ewe was assessed ultrasonographically the day before surgery. Plasma progesterone levels and the quality of the corpora lutea were the criteria for selection of recipients. Ten embryos were transferred to the five selected Spanish Merino recipients, resulting in four pregnancies and seven live-born lambs, including three sets of twins. This study shows that determination of plasma progesterone levels combined with ultrasonographic assessment of the corpus luteum provides information useful for screening of potential recipients. 相似文献
75.
The aim of this study was to evaluate a simple method of monitoring an inflammatory process based on the reaction of normal whole blood to plasma from animals suffering from an induced acute inflammation. There is a strong need to find relevant methods to be able to screen for mediators of inflammatory reactions thereby facilitating diagnosis of inflammatory processes. In e.g. induced endo-toxaemia, the endotoxin disappears very quickly from the circulation and is not a suitable substance to analyse in plasma (Aiumlamaietal 1990). 相似文献
76.
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78.
C. Martínez‐Garza W. Tobon J. Campo H. F. Howe 《Land Degradation \u0026amp; Development》2013,24(3):287-295
Experimental restoration may both accelerate and elucidate natural processes of succession on degraded agricultural land by offering insight into factors that influence rates of succession and the composition of resulting communities. A novel study in restoration of degraded tropical agricultural land in coexistence with cattle ranching activities was established in southern Veracruz, Mexico. The experimental planting of 16 mixed‐species stands of 18 pioneer and late‐successional tree species was established from September to November 2006 on an eroded hillside pasture with an elevational gradient from 182 to 260 m and heterogeneous soil depths. An unusually severe dry season in 2007 killed 72 per cent of the seedlings: least squares regression suggested that survival of six pioneer and 12 late‐successional species was mainly explained by initial basal diameter at planting followed by soil depth for pioneers and by elevational position on the hillside for late‐successional species. Individuals with larger initial size at planting (>4 mm basal diameter), regardless of germination size in a growing house, survived better probably because larger seedlings developed deeper roots that found fissures in substrate underlying thin soils. Interestingly, seedlings small at planting (<4 mm basal diameter) survived as well as large seedlings in deeper (>19 cm) soils, but virtually, all small seedlings died on thin soils (<18·5 cm). Mortality in restoration plantings can be reduced by planting large seedlings of a mix of pioneer and late‐successional species, recognizing that soil depth is a key criterion for survival of the smallest seedlings in a cohort. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
79.
Santiago-Moreno J López-Sebastián A del Campo A González-Bulnes A Picazo R Gómez-Brunet A 《Domestic animal endocrinology》2004,26(4):303-314
The aims of this study were to examine whether mouflons exposed to constant long and short day photoperiods are able to exhibit an annual cycle of hair growth and moult, and prolactin (PRL) secretion. Mouflon ewes were assigned to three groups of treatment. Ewes were maintained, either under natural photoperiod (control, n=9), or received a series of subcutaneous melatonin implants from December to April (n=8), or were exposed to a constant long day photoperiod (16-h light:8-h dark; 16L:8D) during 18 months (n=7). Blood was collected weekly to determine PRL concentrations, and hair samples were clipped weekly from the base of the neck to measure the length of predominant hair. Under constant long days and with melatonin implants, mouflons expressed an annual rhythm of PRL secretion, even though these treatments modified the times of rise or falling of PRL concentrations throughout the year. Hair growth initiation was almost coincident with the summer solstice in both control and melatonin-implanted mouflons but occurred two months earlier in long day hold mouflons (P<0.001). Long day hold mouflons had a lower hair growth rate than control and melatonin-implanted mouflons (P<0.001), and at the end of the experiment, a shorter hair length (3.4±0.24 cm; P<0.01) than control (4.3±0.17 cm), and melatonin-implanted mouflons (4.2±0.12 cm). Our data support the conclusion that in mouflon, an endogenous circannual rhythm of PRL secretion exists, and that the seasonal cycle of hair growth and moult appears to depend, at least in part, on circulating levels of PRL. 相似文献
80.
Del Campo G Santos JI Iturriza N Berregi I Munduate A 《Journal of agricultural and food chemistry》2006,54(8):3095-3100
The low field region (5.8-9.0 ppm) corresponding to aromatic protons and the region 1.8-3.0 ppm of the (1)H NMR spectra were used for characterization and chemometric differentiation of 52 apple juices obtained from six cider apple varieties. The data set consisted of 14 integrated areas corresponding to resonances from acids and phenolic compounds. Multivariate procedures based on hierarchical cluster and discriminant analysis were performed on selected signals of the spectra to determine whether it was possible to distinguish the different juices. Cluster analysis was able to satisfactorily classify the six apple varieties. Discriminant analysis, by means of stepwise procedure for variables selection and leave-one-out for cross-validation, was applied to 40 samples from the year 2001, obtaining recognition and prediction abilities of 100%. The most discriminant variables corresponded to poliphenols, (-)-epicatechin, phloridzin-phloretin, and p-coumaric, chlorogenic, and malic acids. The classification model was applied to 12 samples from apples harvested in the years 2002 and 2003, and the prediction ability was 91.7%. 相似文献