Pain-related cerebral potentials in response to acute painful electrical stimulation in sheep     

JP MORRIS  RM ONG  JK O'DWYER  JL BARNETT  PH HEMSWORTH  IJ CLARKE  EC JONGMAN 《Australian veterinary journal》1997,75(12):883-886

Objective To examine associations between late event-related cerebral potential amplitudes and behavioural responses to noxious electrical stimulation as an indicator of acute pain in sheep.
Design Analysis of variance for the effects of stimulus intensity on the behaviour and event-related cerebral potential variables.
Procedure Ninety-six brief constant current electrical pulse trains were presented to the front left leg of eight sheep at four intensities (2.5 to 10 mA) in a randomised order. An event-related cerebral potential and a graded flinch response were recorded for each stimulus and the 24 event-related cerebral potentials at each intensity were averaged to produce a mean waveform. Various components of this waveform were analysed and changes in these measures and the sheep's flinch response, as stimulus intensity increased, were determined.
Results Both the flinch response and some event-related cerebral potential components, that is, peak amplitudes 114 {N1}, 187 {P1}, 318 {P2} and 230 {Pm} ms after stimulus onset, were significantly affected as stimulus intensity increased.
Conclusion These corresponding behavioural and event-related cerebral potential changes indicate the usefulness of using changes in the event-related cerebral potential to measure acute pain in sheep.  相似文献   

An update on Reproductive Technologies with Potential Short-Term Application in Pig Production     

EA Martinez  JM Vazquez  J Roca  C Cuello  MA Gil  I Parrilla  JL Vazquez 《Reproduction in domestic animals》2005,40(4):300-309

Over the past decade, there has been an increase in the development and/or in the improvement of emerging reproductive technologies in pigs. Among emerging reproductive technologies with potential short-term application in pig production are: artificial insemination with low number of spermatozoa, cryopreservation of spermatozoa and embryos, sperm sexing, and non-surgical embryo transfer. The following review will give emphasis to recent advancements in these reproductive technologies that are starting to show possibilities of serious applications under field conditions.  相似文献   

Three minimum tile paths from bacterial artificial chromosome libraries of the soybean (Glycine max cv. 'Forrest'): tools for structural and functional genomics     

JL Shultz  C Yesudas  S Yaegashi  AJ Afzal  S Kazi  DA Lightfoot 《Plant methods》2006,2(1):9-10

Background  

The creation of minimally redundant tile paths (hereafter MTP) from contiguous sets of overlapping clones (hereafter contigs) in physical maps is a critical step for structural and functional genomics. Build 4 of the physical map of soybean (Glycine max L. Merr. cv. 'Forrest') showed the 1 Gbp haploid genome was composed of 0.7 Gbp diploid, 0.1 Gbp tetraploid and 0.2 Gbp octoploid regions. Therefore, the size of the unique genome was about 0.8 Gbp. The aim here was to create MTP sub-libraries from the soybean cv. Forrest physical map builds 2 to 4.  相似文献   

仔猪梭菌性肠炎的防治经验     

黄建亮 《中国兽药杂志》2006,40(3):56-57

某场饲养的64头仔猪，于10d内发生梭菌性肠炎，采用疫苗免疫和中药治疗进行防治取得了很好的效果，其中中药治疗32例，治愈25例。  相似文献   

Gene Expression and Developmental Competence of Bovine Embryos Produced In Vitro with Different Serum Concentrations     

ATD de  Oliveira  RFF Lopes  JL Rodrigues 《Reproduction in domestic animals》2006,41(2):129-136

  相似文献   

Low-Dose Insemination in Pigs: Problems and Possibilities     

JM Vazquez  J Roca  MA Gil  C Cuello  I Parrilla  I Caballero  JL Vazquez  EA Martínez 《Reproduction in domestic animals》2008,43(S2):347-354

Low-dose AI procedures are required by the pig industry to efficiently utilize emerging sperm technologies, such as cryopreservation and sex-sorting. Currently, several different procedures for inseminating with a low or very low number of spermatozoa have been described. Deep intrauterine insemination allows the deposition of the spermatozoa in the depth of the uterine horn, allowing a significant reduction in the number of spermatozoa inseminated with maintenance of optimal reproductive performance. Intra-oviductal laparoscopic insemination has been recently applied in pigs. This technique has proved to be applicable with diluted and sex-sorted spermatozoa. This review discusses several problems encountered during the development of deep intrauterine insemination and intra-oviductal laparoscopic insemination of pigs and provides potential solutions for the practical application of both the technologies.  相似文献   

Uterine serpin (SERPINA 14) correlates negatively with cytokine production at the foetal–maternal interface but not in the corpus luteum in pregnant dairy heifers experimentally infected with Neospora caninum          下载免费PDF全文

B Serrano‐Pérez  S Almería  R Mur‐Novales  I López‐Helguera  I Garcia‐Ispierto  JL Alabart  L Darwich  F López‐Gatius 《Reproduction in domestic animals》2018,53(2):556-558

This study examines gene expression patterns in dairy heifers experimentally infected with N. caninum during on Day 110 of pregnancy with live foetuses at euthanasia, 42 days later. The study population was constituted of four non‐infected controls and three infected dams. Gene expression was determined on gamma interferon (IFNγ), (Th1 pro‐inflammatory cytokine), interleukin‐4 (IL4) (Th2 pro‐gestation cytokine) or interleukin‐10 (IL10) (T regulatory cytokine) and the serine peptidase inhibitor SERPINA14 in intercaruncular, placental, uterine lymph node (UTLN) and luteal tissue samples. Intercaruncular SERPINA14 expression was negatively correlated with IFNγ expression in cotyledon samples and with IL4 expression in UTLN. No relationships were detected between cytokine gene expression at the foetal–maternal interface and SERPINA14 expression in the luteal samples. Our findings suggest that gene expression of the uterine serpin SERPINA14 correlates negatively with the expression of Th1 and Th2 cytokines at the foetal–maternal interface but not in the corpus luteum.  相似文献   

Monitoring changes in back fat thickness and its effect on the restoration of ovarian activity and fertility in Bos indicus cows          下载免费PDF全文

BR Díaz  CS Galina  I Rubio  M Corro  JL Pablos  A Orihuela 《Reproduction in domestic animals》2018,53(2):495-501

With the objective of testing the hypothesis if animals with a stable layer of body fat (FAT) during the peripartum have a better chance of becoming pregnant after calving, fifty‐nine multiparous Brahman cows in their last trimester of pregnancy were used. Animals averaged four parturitions and were stocked at a rate of 1.25 animal units per hectare and divided into two groups depending on the time postpartum (dpp) that the intravaginal releasing device CIDR was inserted; Group 1 (<30 dpp; n = 30) received the implant at 25.2 ± 4.21 and withdrawn 9 days later. Group 2 (≥30 dpp; n = 29) received the CIDR at 38.41 ± 5.8. Animals were AI at detected oestrus until 170 dpp and calculated as pregnant at first service or requiring more than one service (1s and >1s), not pregnant but cycling (not pregnant) and those not cycling at all (anestrus). The FAT measurements were taken twice each month from the last trimester of gestation until 96 dpp. The onset of ovarian activity was monitored through blood levels of progesterone (P4) at days 14 and 9 prior to CIDR insertion and days 10, 13, 30 and 33 after CIDR withdrawal. Animals pregnant did not have any major changes in their fat thickness. In contrast, cows pregnant in the group ≥30 dpp had changes in their FAT homoeostasis, and pregnant animals in the 1s and >1s groups did not show differences in dorsal back fat in the last trimester of pregnancy and early postpartum. In contrast, animals not pregnant and in anestrus FAT values decreased considerably after parturition. Overall, fertility was 49%, but 18% of all the animals remained anestrus losing FAT. Thus, animals with adequate metabolic conditions will have a better chance of pregnancy regardless of the time postpartum when the reproductive programme starts.  相似文献   

Spatial pattern formation in an insect host-parasitoid system     

JL Maron  S Harrison 《Science (New York, N.Y.)》1997,278(5343):1619-1621

Spatial models in ecology predict that populations may form patchy distributions within continuous habitats, through strong predator-prey or host-parasitoid interactions combined with limited dispersal. Empirical support of these models is provided. Parasitoids emanating from a population outbreak of tussock moths (Orgyia vetusta) suppressed the growth of nearby experimental populations of the moth, while experimental populations farther away were able to grow. This result explains the observed localized nature of tussock moth outbreaks and illustrates how population distributions can be regulated by dynamic spatial processes.  相似文献   

Earth-Based Observations of the Galileo Probe Entry Site     

G Orton  JL Ortiz  K Baines  G Bjoraker  U Carsenty  F Colas  A Dayal  D Deming  P Drossart  E Frappa  J Friedson  J Goguen  W Golisch  D Griep  C Hernandez  W Hoffmann  D Jennings  C Kaminski  J Kuhn  P Laques  S Limaye  H Lin  J Lecacheux  T Martin  G McCabe  T Momary  D Parker  R Puetter  M Ressler  G Reyes  P Sada  J Spencer  J Spitale  S Stewart  J Varsik  J Warell  W Wild  P Yanamandra-Fisher  G Fazio  J Hora  L Deutsch 《Science (New York, N.Y.)》1996,272(5263):839-840

Earth-based observations of Jupiter indicate that the Galileo probe probably entered Jupiter's atmosphere just inside a region that has less cloud cover and drier conditions than more than 99 percent of the rest of the planet. The visual appearance of the clouds at the site was generally dark at longer wavelengths. The tropospheric and stratospheric temperature fields have a strong longitudinal wave structure that is expected to manifest itself in the vertical temperature profile.  相似文献