Isolates of Verticillium dahliae Pathogenic to Crucifers Are of at Least Three Distinct Molecular Types     

Collins A  Okoli CA  Morton A  Parry D  Edwards SG  Barbara DJ 《Phytopathology》2003,93(3):364-376

ABSTRACT Diverse isolates of the soilborne wilt fungi Verticillium dahliae and V. albo-atrum were studied to understand the nature and origins of those infecting cruciferous hosts. All isolates from cruciferous crops produced microsclerotia, and the majority produced long conidia with a high nuclear DNA content; these isolates were divided into two groups by amplified fragment length polymorphism (AFLP) analysis. One group could be subdivided by other criteria such as rRNA sequences and mitochondrial DNA restriction fragment length polymorphism (RFLP) analysis. Two crucifer isolates were short spored and had a low nuclear DNA content. The results are consistent with the crucifer isolates being interspecific hybrids. The long-spored isolates are best regarded as amphihaploids (or allodiploids) with the AFLP groups probably each representing separate interspecific hybridization events. The short-spored crucifer isolates appear to be derived from interspecific hybrids and are here called 'secondary haploids'. Molecular evidence suggests that one parent in the crosses was similar to V. dahliae. The other parent of the amphihaploids seems to have been more similar to V. albo-atrum than to V. dahliae, but was distinct from all isolates of either species so far studied. The implications for the taxonomy of crucifer isolates are discussed and the use of the name V. longisporum, proposed elsewhere for just some of these isolates, is discouraged.  相似文献   

Endometrial Adenocarcinoma and Mucometra in a 6-year-old Alaska Malamute Dog     

FJ Pena  JA Gines  J Duque  V Vieitez  R Martinez –Pérez  L Madejón  I Nuñez Martinez  JM Moran  S Fernández-García 《Reproduction in domestic animals》2006,41(2):189-190

A 6-year-old female Alaska Malamute dog was presented for evaluation of abdominal enlargement referred by a local veterinarian. On the history, the owner complained of chronic abdominal enlargement initiated more than 4 months ago, reduced appetite, occasional vomiting and general dullness. He also complained of greenish mucous intermittent vaginal discharge starting 10 days ago. The bitch was chronically treated with medroxiprogesterone acetate. A laparatomy was performed and fluid in the abdomen was found and aspirated during the surgery. Also a very fluid-filled distended uterus and a mass in the distal part of the left uterine horn were found. The mass was encapsulated by the omentum, but areas of necrosis and calcification were identified. Histopathological diagnosis was endometrial adenocarcinoma.  相似文献   

Apoptosis-independent Poor Morphology of Bovine Embryos Produced by Multiple Ovulation     

S Ikeda  JM Prendes  C Alonso-Montes  A Rodríguez  C Díez  M Kitagawa  H Imai  E Gómez 《Reproduction in domestic animals》2006,41(5):383-385

In multiple ovulation and embryo transfer (MOET) programmes in cattle, a considerable number of morphologically poor-quality embryos continue to be produced; this is one of the limiting factors of the technique. Apoptosis has often been implicated in developmental arrest and fragmentation; these are regarded as poor traits of embryonic quality in mammalian pre-implantation embryos. In the present study, apoptosis was assessed in morphologically poor-quality embryos in comparison with good-quality embryos that were recovered from a MOET programme. Retarded embryos (two to 16 cell stage), morulae with severe fragmentation and morphologically good-quality morulae recovered from superstimulated cows at day 7 post-insemination were subjected to TdT-mediated dUTP nick-end labelling (TUNEL) and Hoechst staining. Cell nuclei that showed both TUNEL staining and apoptotic morphology were considered to be apoptotic. Apoptotic index (AI) was calculated as the percentage of apoptotic cells per embryo. Fifteen of 17 retarded embryos and 10 of 15 morphologically poor-quality morulae did not show signs of apoptosis. The mean AIs in the morphologically poor-quality embryos (two to 16 cell stage, 2.2%; poor morulae, 1.3%) were as low as that in the good-quality embryos (2.9%). These results suggest that another mode of developmental arrest and/or fragmentation that is independent of apoptosis occurs in morphologically poor-quality embryos recovered from MOET programmes.  相似文献   

Effects of Grade of Oocyte–Cumulus Complex and the Interactions Between Grades on the Production of Blastocysts in the Cow, Ewe and Lamb     

JM Kelly  DO Kleemann  SR Rudiger  SK Walker 《Reproduction in domestic animals》2007,42(6):577-582

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Semen Technologies in Dog Breeding: an Update     

FJ Peña  I Núñez-Martínez  JM Morán 《Reproduction in domestic animals》2006,41(S2):21-29

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Attempted transmission of dermatophilosis in saltwater crocodiles (Crocodylus porosus)     

GN BUENVIAJE  PW LADDS  RG HIRST  PM SUMMERS  JM MILLAN 《Australian veterinary journal》1998,76(7):495-496

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Histologic alterations in the testes of laboratory rabbits     

D Morton  S E Weisbrode  W E Wyder  J K Maurer  C C Capen 《Veterinary pathology》1986,23(2):214-217

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Oral extramedullary plasmacytomas in two dogs     

L D Morton  C L Barton  G S Elissalde  S R Wilson 《Veterinary pathology》1986,23(5):637-639

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Juvenile renal disease in miniature schnauzer dogs     

L D Morton  R K Sanecki  D E Gordon  R L Sopiarz  J S Bell  P S Sakas 《Veterinary pathology》1990,27(6):455-458

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Tree water use and rainfall partitioning in a mature poplar-pasture system     

Guevara-Escobar A  Edwards WR  Morton RH  Kemp PD  Mackay AD 《Tree physiology》2000,20(2):97-106

Traditionally, poplars (Populus) have been planted to control erosion on New Zealand's hill-slopes, because of their capacity to dry out and bind together the soil, by reducing effective rainfall and increasing evapotranspiration and soil strength. However, the effect of widely spaced poplars on the partitioning of soil water and rainfall has not been reported. This study determined rainfall partitioning for 18 mid-spring days in a mature P. deltoides (Bart. ex Marsh, Clone I78)-pasture association (37 stems per hectare, unevenly spaced at 16.4 +/- 0.4 m) and compared it with a traditional open pasture system in grazed areas of a hill environment. Tree transpiration was measured by the heat pulse technique. A time-driven mathematical model was used to set a zero offset, adjust anomalous values and describe simultaneous sap velocity time courses of trees. The model showed that daylight sap flow velocities can be represented with a nonlinear Beta function (R(2) > 0.98), and differences in the parameters representing the initiation, duration and conformation of the sap velocity can be tested statistically to discern tree transpiration differences during the day. Evapotranspiration was greater for the poplar-pasture association than for the open pasture (2.7-3.0 versus 2.2 mm day(-1)). The tree canopy alone contributed 0.92 mm day(-1) as transpiration and 1.37 mm day(-1) as interception, whereas evapotranspiration of the pasture understory was only 0.4-0.6 mm day(-1). Despite the higher water use of the poplar-pasture association, soil water in the 0-300 mm soil stratum was higher than, or similar to, that of the open pasture. Tree shading decreased evapotranspiration and pasture accumulation under the trees.  相似文献