Conjunctivitis, tracheitis, and pneumonia associated with herpesvirus infection in green sea turtles   总被引：3，自引：0，他引：3  

E R Jacobson  J M Gaskin  M Roelke  E C Greiner  J Allen 《Journal of the American Veterinary Medical Association》1986,189(9):1020-1023

Fourteen juvenile (15- to 20-month-old) green sea turtles (Chelonia mydas), representative of a group of sea turtles with clinical signs of respiratory tract disease, were euthanatized and submitted for necropsy. Macroscopically, lesions included periglottal necrosis, tracheitis with intraluminal caseous and laminated necrotic debris, and severe pneumonia. Several turtles had caseous conjunctival exudate covering the eyes. Microscopically, the turtles had fibrinonecrotic inflammation around the glottal opening, tracheitis, and severe bronchopneumonia and interstitial pneumonia. In multifocal areas, periglottal and tracheal epithelial cells adjacent to areas of necrosis had hypertrophic nuclei with amphophilic intranuclear inclusions. A mixed population of primarily gram-negative microorganisms was isolated from the tracheal and glottal lesions. Attempts at viral isolation in cultures of green sea turtle kidney cells resulted in the development of cytopathic effects characterized by giant cell formation and development of intranuclear inclusions. Using electron microscopy, intranuclear viral particles (88 to 99 nm in diameter) were seen in inclusion-containing tracheal and glottal epithelial cells and infected green sea turtle kidney cells; particles were consistently seen enveloping from nuclear membranes, and mature particles (132 to 147 nm) were found in the cytoplasm. On the basis of size, conformation, location, and presence of an envelope, the particles most closely resembled those of herpes-viruses.  相似文献   

Serum concentrations of gentamicin in cats   总被引：1，自引：0，他引：1  

E R Jacobson  J M Groff  R R Gronwall  A F Moreland  M Chung 《American journal of veterinary research》1985,46(6):1356-1358

Twenty-one adult cats, allotted into 2 groups, were given gentamicin sulfate at dosages of either 5.0 mg/kg of body weight or 2.5 mg/kg as a single IM injection. During a 24-hour period, serum concentrations of gentamicin were measured serially, using a fluorescence immunoassay. The mean peak serum concentration of gentamicin in cats given 5.0 mg/kg was 23.1 micrograms/ml at postinjection hour (PIH) 0.5; thereafter, the mean serum concentration steadily decreased to 2.0 micrograms/ml at PIH 24. The mean peak serum concentration for cats administered 2.5 mg/kg was 9.1 micrograms/ml at PIH 0.5; thereafter, the mean serum concentration steadily decreased to 1.3 micrograms/ml at PIH 12. Serum therapeutic concentrations, without exceeding toxic concentrations, were attained at the 2.5 mg/kg dosage.  相似文献   

Response of wheat and oat seedlings to root-applied diclofop-methyl and 2,4-dichlorophenoxyacetic acid     

Andrew Jacobson  Richard H. Shimabukuro  Claudia McMichael 《Pesticide biochemistry and physiology》1985,24(1):61-67

Roots of wheat and oat seedlings were treated with diclofop-methyl (methyl 2-[4-(2′,4′-dichlorophenoxy)phenoxy]propanoate) in a specially designed Plexiglas treatment apparatus. Diclofopmethyl severely inhibited the root growth of susceptible oat seedlings but roots of resistant wheat seedlings were unaffected. Diclofop-methyl at 0.3 μM reduced the growth of oat roots to 50% of the control. Direct contact between diclofop-methyl and the inhibited root zone was necessary for growth inhibition since other parts of the seedling (roots and shoots) isolated from contact with diclofop-methyl solution by a physical barrier were unaffected. Diclofop (2-[4-(2′,4′-dichlorophenoxy)phenoxy]propionic acid), the free acid metabolite of diclofop-methyl, was somewhat more phytotoxic than the parent compound. The herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D), which engenders auxin responses, slightly enhanced the inhibition of oat root growth by diclofop-methyl. The primary wheat metabolite, ring-hydroxylated diclofop, was nonphytotoxic to oat root growth, whereas the acetylated derivative of the primary water-soluble oat metabolite (neutral glucose ester of diclofop) inhibited oat root growth to the same extent as diclofop-methyl. These results support the hypothesis that the basis for selectivity between resistant wheat and susceptible oat is the metabolism of diclofop-methyl by aryl hydroxylation and conjugation but not glucose ester conjugation. Translocation is also not an important factor in the phytotoxic activity of diclofop-methyl.  相似文献   

Transmissibility of the contagious equine metritis organism for the cat     

P J Timoney  S J Shin  D H Lein  R H Jacobson 《Comparative immunology, microbiology and infectious diseases》1984,7(2):131-140

A group of SPF cats were moderately susceptible to the causal organism of contagious equine metritis (CEM) following intra-uterine or intrapreputial challenge with an Irish streptomycin resistant strain isolated from a clinically infected mare. Subclinical infections were established in only 50% of the cats, none of which became long-term carriers of the organism. Cytological examination of vaginal smears was of no diagnostic value in confirming infection in inapparently infected cats. Bacteriological responses after primary or secondary challenge with the CEM organism were essentially similar, with one exception, a female cat in which there was possible evidence of local immunity persisting after the primary infection. Efforts to reactivate shedding subsequent to the immediate post-challenge period were unsuccessful. Throughout the experimental period, the cats remained sero-negative to the complement-fixation test, and they failed to develop any significant increase in the levels of antibody activity as measured by the kinetics-based ELISA or KELA system. On day 89 after primary challenge, the cats were euthanized and various sites in the genitourinary tract and the internal iliac lymphatic glands subjected to bacteriological and pathological examination for evidence of CEM infection with negative results. The findings of this study, although establishing the transmissibility of the CEM organism for the cat, demonstrate the limited value of this species as an experimental model system for the disease in the horse.  相似文献   

A comparison of end-tidal halothane concentrations measured at proximal and distal ends of the endotracheal tube in the horse.     

N S Matthews  S M Hartsfield  J L Cornick  J D Jacobson  J D Williams 《The Cornell veterinarian》1992,82(1):21-27

Measurements (n = 126) of end-tidal halothane concentrations were taken from 21 horses anesthetized for routine and emergency surgery. One hundred five paired values allowed comparison of gas samples taken near the oral end of the endotracheal tube (Y1) to samples obtained at the cuffed end of the endotracheal tube (Y2). Twenty-one paired readings were assessed to compare samples taken 25 cm beyond the cuffed end of the tube (Y3) to samples from Y1. Measurements were made at all locations at 15-minute intervals starting 30 minutes after beginning halothane. All measurements were made in triplicate at end-expiration, and both sites were sampled within 1 minute of each other. Halothane concentration was measured by rapid infrared analysis with a gas sampling rate of 150 ml/min and displayed as a digital reading. Calibration of the machine was checked regularly. The difference between readings (Y1-Y2 or Y1-Y3 = Sdif) was tested using general linear models and a significance level of p less than 0.05 was used. The variable Sdif was analyzed with respect to time, mode of ventilation, and type of recumbency; no effects of these variables were detected. The mean values (+/- SD) of 105 readings for Y1 and Y2 were 2.41 vol% (+/- 0.49) and 2.39 vol% (+/- 0.49) respectively, and the Pearson's correlation coefficient (Y1 vs Y2) was 0.96. The mean values (+/- SD) of 21 measurements for Y1 and Y3 were 2.31 vol% (+/- 0.27) and 2.32 vol% (+/- 0.28) respectively and the Pearson's correlation coefficient (Y1 vs Y3) was 0.98.  相似文献   

Electron microscopy of a spiral-shaped bacterium in the blood and bone marrow of a rhinoceros iguana.          下载免费PDF全文

C F Simpson  E R Jacobson    J W Harvey 《Canadian journal of veterinary research》1981,45(4):388-391

Spiral shaped bacteria present in blood smears and bone marrow of a sick rhinoceros iguana were examined by light and electron microscopy. The organisms averaged 10 micron in length and had at least three spiral turns. The cell contained nuclear areas, vacuoles and ribosomes, except at the poles where there was a virtual absence of organelles. The bacterium was found by a cell membrane, cell wall and enveloping sheath. "Blebs" were present with regularity on the cell surface. About 14 flagella were present at each pole, and at these sites there was a specialized thickening of the cell membrane. Organisms were present within a phagocytic vacuole of macrophages in the blood and bone marrow, and often these engulfed organisms were degenerated. The taxonomic position of the bacterium is unknown.  相似文献   

Toward a model for local stakeholder participation in landscape-level wildlife conservation     

Catherine E. Doyle-Capitman  Daniel J. Decker  Cynthia A. Jacobson 《Human Dimensions of Wildlife》2018,23(4):375-390

The conservation community is increasingly taking a landscape-level approach to wildlife conservation. This approach faces obstacles, including how to ensure transboundary wildlife conservation goals that can be realized through local management actions, and how and when to engage local stakeholders in landscape conservation planning processes. Currently, local stakeholder participation is not institutionalized within these processes. This paper examines the potential role of local stakeholders in landscape conservation. In this paper, we present a preliminary model of cross-level coordination that could facilitate development and implementation of conservation across social–ecological landscapes, highlighting the possible contributions of local stakeholders. The feasibility of this model is contingent on addressing a range of challenges associated with local stakeholder participation in extra-local conservation decisions. Ultimately, we present suggestions for future research examining the potential for a locally inclusive, participatory approach to wildlife conservation across complex landscapes.  相似文献   

Poxlike skin lesions in captive caimans.     

E R Jacobson  J A Popp  R P Shields  J M Gaskin 《Journal of the American Veterinary Medical Association》1979,175(9):937-940

Three juvenile captive spectacled caimans (Caiman sclerops) had scattered, gray-white, circular, 1- to 3-mm skin lesions. In one caiman, the lesions were particularly prominent on the phalanges, palpebrae, and integument overlying the maxillae and mandible. Digital biopsy revealed large eosinophilic intracytoplasmic inclusions within epithelial cells. Transmission electron microscopy of lesions from the lower jaw revealed viral particles morphologically similar to poxvirus.  相似文献   

Bilateral palpebral reduction and concurrent mycoplasmosis in a wild Agassiz's desert tortoise (Gopherus agassizii)     

Elliott R. Jacobson  Kristin H. Berry  Dennis E. Brooks  John F. Roberts 《Veterinary ophthalmology》2023,26(4):361-366

A wild Agassiz's desert tortoise, Gopherus agassizii, with bilateral eyelid reduction and plaques of tissue covering the superior surface of both corneas was examined in the field and subsequently submitted to the University of Florida for diagnostics. Polymerase chain reaction (PCR), from a swab of both corneas, was positive for Mycoplasma agassizii. Two months later, the tortoise was euthanatized and necropsied. There was increased bulbar exposure associated with dermal excoriation of periocular scales in both superior and inferior palpebra resulting in an increased palpebral fissure opening. Concurrently, there was bilateral conjunctivitis of the nictitating membranes and squamous metaplasia of the bulbar conjunctiva. Using PCR, Mycoplasma testudineum, another pathogen of tortoises, was identified in both nasal cavities, and the upper respiratory tract histopathological findings were consistent with those described for M. testudineum in Agassiz's desert tortoises. Although eye disease has been reported in desert and gopher (Gopherus polyphemus) tortoises with mycoplasmosis, widespread loss of palpebral tissue, conjunctivitis of the nictitans, and squamous metaplasia of the bulbar conjunctiva have not been reported in tortoises.  相似文献   

Ecosystem management in the Southeast United States: Interest of forest landowners in joint management across ownerships     

Michael G. Jacobson 《Small-Scale Forestry》2002,1(1):71-92

In the south-east United States, 70% of the forest area is privately owned, by an estimated 5 M landholders. If ecosystem or landscape-level management is to work, cooperation across private ownerships is essential. As a first step in garnering cooperation among landowners, a mail survey was carried out to understand their characteristics, attitudes, beliefs and interest in ecosystem management. A specific area of the Coastal Plain of South Carolina, typical of rural forest-dominated areas of this region, was chosen as the study site. Forestry-related uses, specifically timber production, are the main reasons why over half the respondents own their land. Only one-third of the respondents are familiar with the concept of ecosystem management. However, about 70% are interested in learning more about joint management with other landowners. The major concerns about participating in joint management are loss of land and timber values. Most of the landowners who would participate in a landscape corridor system, for example, want to maintain control of their land rights and limit outside intervention in the process.  相似文献