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11.
Chetukuri?Anuradha Pooran?M.?GaurEmail author Suresh?Pande Kishore?K.?Gali Muthyl?Ganesh Jagdish?Kumar Rajeev?K.?Varshney 《Euphytica》2011,182(1):1-9
Botrytis grey mould (BGM) caused by Botrytis cinerea Pers. ex. Fr. is the second most important foliar disease of chickpea (Cicer arietinum L.) after ascochyta blight. An intraspecific linkage map of chickpea consisting of 144 markers assigned on 11 linkage groups
was constructed from recombinant inbred lines (RILs) of a cross that involved a moderately resistant kabuli cultivar ICCV
2 and a highly susceptible desi cultivar JG 62. The length of the map obtained was 442.8 cM with an average interval length
of 3.3 cM. Three quantitative trait loci (QTL) which together accounted for 43.6% of the variation for BGM resistance were
identified and mapped on two linkage groups. QTL1 explained about 12.8% of the phenotypic variation for BGM resistance and
was mapped on LG 6A. It was found tightly linked to markers SA14 and TS71rts36r at a LOD score of 3.7. QTL2 and QTL3 accounted
for 9.5 and 48% of the phenotypic variation for BGM resistance, respectively, and were mapped on LG 3. QTL 2 was identified
at LOD 2.7 and flanked by markers TA25 and TA144, positioned at 1 cM away from marker TA25. QTL3 was a strong QTL detected
at LOD 17.7 and was flanked by TA159 at 12 cM distance on one side and TA118 at 4 cM distance on the other side. This is the
first report on mapping of QTL for BGM resistance in chickpea. After proper validation, these QTL will be useful in marker-assisted
pyramiding of BGM resistance in chickpea. 相似文献
12.
Jayaprakasha GK Jagan Mohan Rao L Sakariah KK 《Journal of agricultural and food chemistry》2000,48(9):4294-4295
The steam-distilled oil of cinnamon (Cinnamomum zeylanicum) flowers was analyzed by GC and GC-MS. It consists of 23% hydrocarbons and 74% oxygenated compounds. A total of 26 compounds constituting approximately 97% of the oil were characterized. (E)-Cinnamyl acetate (41.98%), trans-alpha-bergamotene (7.97%), and caryophyllene oxide (7.2%) are found to be major compounds. This is the first report on the chemical composition of the flower oil of Cinnamomum zeylanicum. 相似文献
13.
Mapping and validation of QTLs for resistance to an Indian isolate of Ascochyta blight pathogen in chickpea 总被引:1,自引:0,他引:1
Pratibha Kottapalli Pooran M. Gaur Sanjay K. Katiyar Jonathan H. Crouch Hutokshi K. Buhariwalla Suresh Pande Kishore K. Gali 《Euphytica》2009,165(1):79-88
Ascochyta blight (AB) caused by Ascochyta rabiei, is globally the most important foliar disease that limits the productivity of chickpea (Cicer arietinum L.). An intraspecific linkage map of cultivated chickpea was constructed using an F2 population derived from a cross between an AB susceptible parent ICC 4991 (Pb 7) and an AB resistant parent ICCV 04516. The
resultant map consisted of 82 simple sequence repeat (SSR) markers and 2 expressed sequence tag (EST) markers covering 10
linkage groups, spanning a distance of 724.4 cM with an average marker density of 1 marker per 8.6 cM. Three quantitative
trait loci (QTLs) were identified that contributed to resistance to an Indian isolate of AB, based on the seedling and adult
plant reaction. QTL1 was mapped to LG3 linked to marker TR58 and explained 18.6% of the phenotypic variance (R
2) for AB resistance at the adult plant stage. QTL2 and QTL3 were both mapped to LG4 close to four SSR markers and accounted
for 7.7% and 9.3%, respectively, of the total phenotypic variance for AB resistance at seedling stage. The SSR markers which
flanked the AB QTLs were validated in a half-sib population derived from the same resistant parent ICCV 04516. Markers TA146
and TR20, linked to QTL2 were shown to be significantly associated with AB resistance at the seedling stage in this half-sib
population. The markers linked to these QTLs can be utilized in marker-assisted breeding for AB resistance in chickpea. 相似文献
14.
To acquire the fertilizing competence, spermatozoa must undergo a cascade of physiological and biochemical changes collectively defined as capacitation. Compelling evidence signifies that the global increase in protein tyrosine phosphorylation is the driving factor for capacitation. In our laboratory, we previously demonstrated that nitric oxide (NO) induces capacitation in buffalo sperm and is associated with an increase in protein tyrosine phosphorylation. The aim of the present study is to identify the proteins undergo tyrosine phosphorylation during NO induced buffalo sperm capacitation using 2-D immunoblotting and mass spectrometry. The percentage of progressively motile and capacitated sperm was more in presence of l-arginine. Along with known tyrosine phosphoproteins like ATP synthase subunit beta, pyruvate dehydrogenase E1 component subunit beta, GST mu 3, F-actin capping protein subunit beta 2, GPD2 and VDAC2, interestingly novel tyrosine phosphoprotein substrates such as actin, serine/threonine-protein phosphatase PP1-gamma catalytic subunit, and glutamine synthetase were also identified which might be specific to the NO induced signaling and also emphasizes the species specificity with respect to tyrosine phosphorylation of proteins during capacitation. In conclusion, this study forms an essential step in delineating the proteins undergo tyrosine phosphorylation in response to NO induced signaling pathways during capacitation of buffalo sperm. 相似文献