Use of ultrasonography to confirm epidural catheter position in a cat     

Hanna Machin  Tristan Merlin  Jaime Viscasillas 《Veterinary anaesthesia and analgesia》2018,45(5):711-713

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A sonographic investigation for the development of ultrasound-guided paravertebral brachial plexus block in dogs: cadaveric study     

Paolo Monticelli  Ella Fitzgerald  Jaime Viscasillas 《Veterinary anaesthesia and analgesia》2018,45(2):195-202

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Evidence of Migration and Endophytic Presence of Agrobacterium tumefaciens in Rose Plants   总被引：2，自引：0，他引：2  

Rubén Martí  Jaime Cubero  Antonio Daza  Jaime Piquer  Carmen I. Salcedo  Clara Morente  María M. López 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(1):39-50

Agrobacterium tumefaciens was isolated from stem tumors of several rose cultivars showing that the bacterium is the causal agent of aerial galls in rose plants. No differences were observed in the characteristics of the Agrobacterium isolates from crown or aerial galls. Stem inoculation of ten rose cultivars showed that all of them were susceptible to A. tumefaciens but differences in the size of the resulting tumors were observed. The movement of A. tumefaciens in rose plants was demonstrated using two wild type strains and two antibiotic resistant mutants. Three months after inoculation, the inoculated strains were recovered in the roots, crown and below and above the inoculation site but low numbers of pathogenic Agrobacterium cells were isolated. New tumors appeared in 5% of the noninoculated wounds. A. tumefaciens was isolated from the stem at different distances from the tumor in naturally infected plants. In symptomless commercial plants, the isolation from the roots, crown and at different stem levels demonstrated the existence of systemic and latent infections in rose. Direct isolation using a nonselective and selective media with or without a previous enrichment step were efficient methods for isolating tumorigenic Agrobacterium from the different parts of rose plants.  相似文献   

Evaluation of the Microalgae Paste Viability Produced in a Mollusk Hatchery in Southern Brazil     

Moira Nunes  Adriana Pereira  Jaime Fernando Ferreira  Fanny Yasumaru 《Journal of the World Aquaculture Society》2009,40(1):87-94

The present study was conducted to define a methodology to produce and store small‐scale microalgae paste to be used in a mollusk hatchery. Microalgae were cultured in 500 L fiberglass tanks, under temperature of 20 ± 2 C, Guillard f/2 culture medium, and continuous light intensity of 203–226 μmol photons/m²/sec. Cultures were centrifuged at 2000 g at the exponential growth phase. Microalgae cell quality after centrifugation and during storage was determined by analyses with Evan’s blue stain and by counting the number of total marine bacteria. Treatments with and without additive were applied to the microalgae paste produced, which was distributed into 100 mL plastic containers, capped, and stored under refrigeration at 4 ± 1 C. Results indicated that in the Chaetoceros muelleri paste, centrifugation did not damage the cells and the number of total marine bacteria reduced significantly from 2.9 × 10⁶ to 8.3 × 10⁵ colony‐forming units per milliliter. Chaetoceros muelleri and Chaetoceros calcitrans pastes stored with addition of 0.1% ascorbic acid had a shelf life shorter than 2 wk. For the treatment without additive, results with Evan’s blue stain showed that cells (99%) remained viable until the sixth week of storage for C. muelleri and seventh week of storage for Skeletonema sp. and C. calcitrans. The number of bacteria did not increase during storage for C. calcitrans and Skeletonema (P > 0.05). For C. muelleri, an increase in bacteria (P < 0.05) was observed after the sixth week of storage. This study demonstrated the feasibility to produce and store microalgae paste for a period of 2–8 wk, which allows it to be used as food source and also optimizes the use of microalgae cultured in laboratory.  相似文献   

Simultaneous effect of sex and dose on efficacy of clove oil,tricaine methanesulfonate, 2‐phenoxyethanol and propofol as anaesthetics in guppies,Poecilia reticulata (Peters)          下载免费PDF全文

Olga Mitjana  Cristina Bonastre  María Teresa Tejedor  Laura Garza  Jaime Esteban  María Victoria Falceto 《Aquaculture Research》2018,49(6):2140-2146

We studied the simultaneous effect of sex and dose on anaesthesia efficacy to estimate, if possible, the lowest effective dose (LED) for clove oil, tricaine methanesulphonate (MS‐222), 2‐phenoxyethanol (2‐PE) and propofol in mature guppies. LED is the lowest dose needed to reach A5 stage in a mean time of 3 min, with mean recovery (R5) time of 5 min. We used four doses/anaesthetic: 25, 50, 75 and 100 mg/L for clove oil; 120,140,160 and 180 mg/L for MS‐222; 800, 1,000, 1,200 and 1,400 mg/L for 2‐PE, and 7.50, 8.25, 10.00 and 11.25 mg/L for propofol. Each dose was tested on 10 females and 10 males. Morbidity, mortality and behavioural changes were checked on two control groups (10 males and 10 females/group). Sedation (A3), A5 and R5 times were recorded. Significant interactive effect dose*sex on A5 time was found for each anaesthetic agent (p_dose*sex < .05). Except for MS‐222 (p_dose*sex = .284), significant interactive effect dose * sex on R5 time was found (p_dose*sex < .05). A5 time in females tended to be greater than in males, but, in general, R5 times were longer in males. Body size differences between males and females could explain these differences in MS‐222 on A5 time and for clove oil, 2‐PE and propofol on R5 time. No dose simultaneous meet LED′s conditions relating to both A5 and R5 times; therefore the lowest doses inducing A5 in a mean time of 3 min could be a safe guideline for anaesthetic procedure in both male and females.  相似文献   

Physicochemical and Microbiological Changes in Dried Small-Spotted Catshark (Scyliorhynus canicula): Contributing to the Developing an Alternative Shark-Based Salted-Dried Seafood Product     

Eduardo Esteves  Hugo Lourenço  Igor Rosa  Jaime Aníbal 《Journal Of Aquatic Food Product Technology》2018,27(2):176-184

Our aim is to contribute to the valorization and optimization of a salted-dried seafood product prepared from small-spotted catshark (Scyliorhynus canicula) akin to “litão seco,” the high-priced, traditional dried blackmouth catshark (Galeus melastomus). This work focused on studying the changes in physicochemical and microbiological parameters of S. canicula salted (for 3 h or 24 h) and dried (convection oven for 24 h or tray drier for 3 h). As expected, salting and drying reduced the water content and water activity, while chloride content in fillets increased. Also, significant but distinct changes in color, namely CIE L* and b*, were observed. Total viable counts were significantly reduced in the salted-dried shark, but the later still presented Staphylococcus aureus, though at marginally acceptable, non-hazardous levels. The abundance of yeasts was reduced by 3 log(cfu/g) in samples oven-dried for 24 h but only by 0.8 log after 3 h in the tray-drier. From our results, salted-dried S. canicula has the required physicochemical characteristics and microbiological quality to be apt for human consumption and is a suitable candidate for becoming a traditional seafood product comparable to “litão seco.”  相似文献   

Extractants for available boron in limed acid soils and its correlation in crops with high carbon storage capacity (Tectona grandis)     

Jaime L. Mercado  Enrique M. Combatt 《Journal of plant nutrition》2016,39(14):2136-2143

The objective of this work was to evaluate the efficiency of six extractants for available boron in acid soils undergoing to liming, and to correlate the results of soil analysis with foliar response seen in the teak plants in greenhouse conditions. Acid soil samples were collected and their chemical characterization was performed using standard analysis protocols. Later, agricultural lime was added to the experimental units in greenhouse conditions in order to control the soil acidity and these were incubated for 30 days. Six extracts for available boron were evaluated and correlated with response in the plant by foliar analysis. It is concluded that a high dissolution power of boron extractant solution produces a high sensitive in the determination of the changes of boron concentration in the limed acid soil. These methods are seen to be not adequate for the determination of available boron contents at high concentrations.  相似文献   

Bait station devices can improve mass trapping performance for the control of the Mediterranean fruit fly          下载免费PDF全文

Vicente Navarro‐Llopis  Jaime Primo  Sandra Vacas 《Pest management science》2015,71(7):923-927

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Optimization of the extraction of antioxidants from Dunaliella salina microalga by pressurized liquids     

Herrero M  Jaime L  Martín-Alvarez PJ  Cifuentes A  Ibáñez E 《Journal of agricultural and food chemistry》2006,54(15):5597-5603

In this work, extraction of antioxidant compounds from Dunaliella salina microalga is optimized by combining pressurized liquid extraction (PLE) and experimental design (three-level factorial design) with three different solvents (hexane, ethanol, and water). Two main factors were considered, the extraction temperature (40, 100, and 160 degrees C) and the extraction time (5, 17.5, and 30 min). As response variables, the extraction yield (percent dry weight/initial weight) and the antioxidant activity of the extracts (determined using the TEAC method) were used. The parameters of the model were estimated by multiple linear regression. Results showed that the extraction temperature was the factor having the strongest influence (positive) on the two response variables. The best yields were obtained with ethanol at the higher extraction temperature and time tested. Besides, although hexane extracts provided the best antioxidant activity, ethanol extracts were also very active. The chemical characterization of ethanol extracts was carried out using HPLC-DAD, and attempts have been made to correlate their chemical composition with the antioxidant activity measured. Results pointed out that the extracts contained, besides all-trans-beta-carotene and isomers, several different minor carotenoids that seemed to make a contribution to the antioxidant activity of the extracts.  相似文献   

Gallbladder aspirate from a dog     

Neel JA  Tarigo J  Grindem CB 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(4):467-470

A 7-year-old, male, castrated, Labrador Retriever with a history of pancreatitis and inflammatory bowel disease presented for vomiting and anorexia. Serum biochemistry findings were indicative of cholestasis, hepatocellular insult, and decreased hepatic function. Ultrasound examination showed sediment and gas within the gallbladder, and a diagnosis of emphysematous cholecystitis was made. Emergency gallbladder resection was performed. Cytologic examination of bile fluid collected at surgery showed a mixed population of bacteria (bactibilia) together with fungal organisms consistent with Cyniclomyces guttulatus (previously known as Saccharomycopsis guttulatus). Similar fungal organisms were seen on a fecal smear. Bacteria cultured were normal gastrointestinal flora, supporting ascending infection; the fungal organisms were interpreted as incidental. Histopathology of the gallbladder indicated active (suppurative) and chronic (lymphocytic) cholecystitis and sections of liver tissue had evidence of chronic liver disease. A positive liver culture indicated concurrent bacterial hepatitis or cholangiohepatitis. Despite supportive care, the dog continued to decline and was euthanized 30 days later. Necropsy results confirmed end stage liver disease, but an initiating cause was not found. This case highlights the role of bactibilia in the development of acute cholecystitis and the unique cytologic appearance of C guttulatus as an incidental finding in bile fluid.  相似文献