全文获取类型
收费全文 | 2038篇 |
免费 | 110篇 |
国内免费 | 12篇 |
专业分类
林业 | 168篇 |
农学 | 107篇 |
基础科学 | 25篇 |
519篇 | |
综合类 | 162篇 |
农作物 | 90篇 |
水产渔业 | 149篇 |
畜牧兽医 | 751篇 |
园艺 | 47篇 |
植物保护 | 142篇 |
出版年
2024年 | 4篇 |
2023年 | 7篇 |
2022年 | 19篇 |
2021年 | 39篇 |
2020年 | 60篇 |
2019年 | 56篇 |
2018年 | 64篇 |
2017年 | 64篇 |
2016年 | 73篇 |
2015年 | 51篇 |
2014年 | 84篇 |
2013年 | 131篇 |
2012年 | 122篇 |
2011年 | 141篇 |
2010年 | 104篇 |
2009年 | 104篇 |
2008年 | 145篇 |
2007年 | 127篇 |
2006年 | 102篇 |
2005年 | 92篇 |
2004年 | 92篇 |
2003年 | 84篇 |
2002年 | 103篇 |
2001年 | 34篇 |
2000年 | 19篇 |
1999年 | 17篇 |
1998年 | 17篇 |
1997年 | 14篇 |
1996年 | 15篇 |
1995年 | 27篇 |
1994年 | 10篇 |
1993年 | 12篇 |
1992年 | 8篇 |
1991年 | 9篇 |
1990年 | 7篇 |
1989年 | 7篇 |
1988年 | 8篇 |
1987年 | 10篇 |
1985年 | 14篇 |
1984年 | 9篇 |
1983年 | 6篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1974年 | 3篇 |
1971年 | 4篇 |
排序方式: 共有2160条查询结果,搜索用时 15 毫秒
31.
采用二维电泳技术对硝酸银诱导的大豆子叶微粒体膜蛋白进行分离,利用四极杆-飞行时间串联质谱,对仅存在于诱导样品而不存在于对照样品中的蛋白质点进行氨基酸序列测定和分析,以期研究硝酸银处理对大豆子叶微粒体蛋白质的影响。结果显示:硝酸银可以诱导大豆Kunitz胰蛋白酶抑制剂(SKTI)的产生,在诱导处理的子叶中大量存在其成熟形式,也存在其5 kDa小亚基,小亚基含有“GIGTIISSPER”和“FIAEGNPLR”氨基酸序列。该研究结果为SKTI的深入研究提供了实践依据,并为其分离纯化提供了简便途径,从而有利于对其更多性质的进一步研究。 相似文献
32.
33.
Sabine Augustin Jan Evers Hans-Peter Dietrich Johannes Eichhorn Thomas Haussmann Regina Icke Ansgar Isenberg Wolfgang Lux Monica Musio Hans Pretzsch Winfried Riek Thomas Rötzer Bernd Schultze Andreas Schulze Jörg Schröder Walter Seidling Nicole Wellbrock Klaus von Wilpert Barbara Wolff 《European Journal of Forest Research》2005,124(4):251-260
In the 1970s unexpected forest damages, called “new type of forest damage” or “forest decline”, were observed in Germany and
other European countries. The Federal Republic of Germany and the German Federal States implemented a forest monitoring system
in the early 1980s, in order to monitor and assess the forest condition. Due to the growing public awareness of possible adverse
effects of air pollution on forests, in 1985 the ICP Forests was launched under the convention on long-range transboundary
air pollution (CLRTAP) of the United Nations Economic Commission for Europe (UN-ECE). The German experience in forest monitoring
was a base for the implementation of the European monitoring system. In 2001 the interdisciplinary case study “concept and
feasibility study for the integrated evaluation of environmental monitoring data in forests”, funded by the German Federal
Ministry of Education and Research, concentrated on in-depths evaluations of the German data of forest monitoring. The objectives
of the study were: (a) a reliable assessment of the vitality and functioning of forest ecosystems, (b) the identification
and quantification of factors influencing forest vitality, and (c) the clarification of cause-effect-relationships leading
to leaf/needle loss. For these purposes additional data from external sources were acquired: climate and deposition, for selected
level I plots tree growth data, as well as data on groundwater quality. The results show that in particular time series analysis
(crown condition, tree growth, and tree ring analysis), in combination with climate and deposition are valuable and informative,
as well as integrated evaluation of soil, tree nutrition and crown condition data. Methods to combine information from the
extensive and the intensive monitoring, and to transfer process information to the large scale should be elaborated in future.
相似文献
Sabine AugustinEmail: |
34.
基于GIS技术开发的公路景观管理决策支持系统是针对公路景观管理业务而开发的信息管理系统,系统采用地理信息系统(GIS)、专家系统(ES)以及数据库管理系统相结合的方法,建立了公路两侧自然本底数据、多媒体数据、评价指标因子、恢复方案等为主体的属性数据库;公路空间数据库;由专家知识及经验构成的知识库;相关的评价模型建立模型库和决策库;实现了多库统一的地理信息系统与专家系统相结合的决策支持系统。为公路景观管理部门对公路景观动态管理、评价公路自身及景观现状、制定恢复对策和措施提供了科学实用的管理手段。 相似文献
35.
36.
Dongchen An Ernesto Lopes Pinheiro-Junior Lszl Bress Irina Gladkikh Elena Leychenko Eivind A. B. Undheim Steve Peigneur Jan Tytgat 《Marine drugs》2022,20(2)
(1) Background: G protein-coupled inward-rectifier potassium (GIRK) channels, especially neuronal GIRK1/2 channels, have been the focus of intense research interest for developing drugs against brain diseases. In this context, venom peptides that selectively activate GIRK channels can be seen as a new source for drug development. Here, we report on the identification and electrophysiological characterization of a novel activator of GIRK1/2 channels, AsKC11, found in the venom of the sea anemone Anemonia sulcata. (2) Methods: AsKC11 was purified from the sea anemone venom by reverse-phase chromatography and the sequence was identified by mass spectrometry. Using the two-electrode voltage-clamp technique, the activity of AsKC11 on GIRK1/2 channels was studied and its selectivity for other potassium channels was investigated. (3) Results: AsKC11, a Kunitz peptide found in the venom of A. sulcata, is the first peptide shown to directly activate neuronal GIRK1/2 channels independent from Gi/o protein activity, without affecting the inward-rectifier potassium channel (IRK1) and with only a minor effect on KV1.6 channels. Thus, AsKC11 is a novel activator of GIRK channels resulting in larger K+ currents because of an increased chord conductance. (4) Conclusions: These discoveries provide new insights into a novel class of GIRK activators. 相似文献
37.
Ernesto Lopes Pinheiro-Junior Rimma Kalina Irina Gladkikh Elena Leychenko Jan Tytgat Steve Peigneur 《Marine drugs》2022,20(2)
Sea anemones are a rich source of biologically active compounds. Among approximately 1100 species described so far, Heteractis crispa species, also known as sebae anemone, is native to the Indo-Pacific area. As part of its venom components, the Hcr 1b-2 peptide was first described as an ASIC1a and ASIC3 inhibitor. Using Xenopus laevis oocytes and the two-electrode voltage-clamp technique, in the present work we describe the remarkable lack of selectivity of this toxin. Besides the acid-sensing ion channels previously described, we identified 26 new targets of this peptide, comprising 14 voltage-gated potassium channels, 9 voltage-gated sodium channels, and 3 voltage-gated calcium channels. Among them, Hcr 1b-2 is the first sea anemone peptide described to interact with isoforms from the Kv7 family and T-type Cav channels. Taken together, the diversity of Hcr 1b-2 targets turns this toxin into an interesting tool to study different types of ion channels, as well as a prototype to develop new and more specific ion channel ligands. 相似文献
38.
39.
Stephen J. Dugdale Jan Franssen Emily Corey Normand E. Bergeron Michel Lapointe Richard A. Cunjak 《Ecology of Freshwater Fish》2016,25(3):429-445
Atlantic salmon become thermally stressed when water temperatures exceed 23 °C. To alleviate this stress, they behaviourally thermoregulate by moving to patches of cold water, often forming large aggregations. These patches are known as thermal refuges. Given the consensus that climate change will increase temperatures in Atlantic salmon catchments, thermal refuges will become increasingly important in minimising summer mortalities. While the behaviour of salmonids within thermal refuges is fairly well understood, less is known about their main stem movement in search of thermal refuges or its thermal and temporal cues. We detail the results of a PIT telemetry study to investigate the main stem movement behaviour of thermally stressed Atlantic salmon parr in a temperature‐impacted river. PIT antennas placed around two thermal refuges and at the upstream and downstream limits of their surrounding reach were used to record the movement of salmonids during a heatwave. We observed parr movement at the upstream and downstream antennas 135 min prior to the occurrence of the midpoint of aggregations in the thermal refuges, indicating that Atlantic salmon parr make reach‐scale movements in search of cool water prior to aggregating. Logistic regression showed that the number of degree hours >28 °C predicted the occurrence of main stem movement with a good degree of accuracy, indicating that this temperature represents a fundamental threshold causing Atlantic salmon parr to move towards cool water. Such data could be instrumental in allowing river managers to place limits on human activity within rivers, allowing salmon populations time to recover following heat stress events. 相似文献
40.
Michal Janáč Luděk Šlapanský Zdenka Valová Pavel Jurajda 《Ecology of Freshwater Fish》2013,22(3):430-438
Several Ponto‐Caspian gobiid species have recently expanded their ranges in Europe and North America. This is the first study to demonstrate passive downstream dispersal (drift) of the round goby (Neogobius melanostomus) and tubenose goby (Proterorhinus semilunaris) in newly colonised areas (River Dyje, Danube basin), a factor that could play an important role in their dispersal. Drift duration (i.e. number of dates on which the species was caught) in round goby was longer than that of both native species and tubenose goby, providing a possible advantage. Size of drifting fish was restricted to a narrow range of 6–8 mm and 5–8 mm for round and tubenose goby, respectively. Drift in both species occurred almost completely during hours of darkness (<1 lux) and fish size did not vary throughout the night. In both species, drift density increased significantly during the first hour after dusk. Round goby density gradually decreased up to dawn, while tubenose goby density varied throughout the night with no clear pattern. Drift of early life stages appears to be an important phenomenon that has not received adequate attention in studies of round and tubenose goby ecology and dispersal. 相似文献