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排序方式: 共有163条查询结果,搜索用时 93 毫秒
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A national serological survey to verify Australia's freedom from porcine reproductive and respiratory syndrome 总被引:4,自引:0,他引:4
MG GARNER LJ GLEESON PK HOLYOAKE RM CANNON WJ DOUGHTY 《Australian veterinary journal》1997,75(8):596-600
Objective To provide serological data to support Australia's claim of freedom from porcine reproductive and respiratory syndrome.
Design A national serological survey was designed to provide 99% confidence of detecting at least one infected pig herd in Australia, assuming that at least 5% of herds would have been exposed to porcine reproductive and respiratory syndrome virus and that at least 25% of the 'finisher' pigs in these herds would have antibodies to the virus.
Procedure A two-stage testing regime was used. All samples were tested with a commercially available enzyme-linked immunosorbent assay. If assay reactions were found, all samples from the herd were to be tested using the indirect immunofluorescence antibody assay.
Results Of the 875 samples from 163 herds from all States in Australia, there was some evidence of reactivity in only four samples from four herds on the enzyme-linked immunosorbent assay. Further testing using the indirect immunofluorescence antibody assay according to the study protocol demonstrated that the reactions were not due to the presence of specific porcine reproductive and respiratory syndrome virus antibodies in the sera.
Conclusion The results of this study support the view that Australian pigs are free of porcine reproductive and respiratory syndrome virus. 相似文献
Design A national serological survey was designed to provide 99% confidence of detecting at least one infected pig herd in Australia, assuming that at least 5% of herds would have been exposed to porcine reproductive and respiratory syndrome virus and that at least 25% of the 'finisher' pigs in these herds would have antibodies to the virus.
Procedure A two-stage testing regime was used. All samples were tested with a commercially available enzyme-linked immunosorbent assay. If assay reactions were found, all samples from the herd were to be tested using the indirect immunofluorescence antibody assay.
Results Of the 875 samples from 163 herds from all States in Australia, there was some evidence of reactivity in only four samples from four herds on the enzyme-linked immunosorbent assay. Further testing using the indirect immunofluorescence antibody assay according to the study protocol demonstrated that the reactions were not due to the presence of specific porcine reproductive and respiratory syndrome virus antibodies in the sera.
Conclusion The results of this study support the view that Australian pigs are free of porcine reproductive and respiratory syndrome virus. 相似文献
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The occurrence of Haemophilus somnus in feedlot calves and its control by postarrival prophylactic mass medication. 总被引:7,自引:5,他引:2 下载免费PDF全文
J Van Donkersgoed E D Janzen A A Potter R J Harland 《The Canadian veterinary journal. La revue veterinaire canadienne》1994,35(9):573-580
Three field trials were conducted in a large commercial feedlot in Saskatchewan to determine the prevalence of Haemophilus somnus in calves and to evaluate the efficacy of prophylactic mass medication with long-acting oxytetracycline on day 17 (1990, n = 1336), day 11 (1991, n = 4372), or day 8 (1992, n = 5632) postarrival. Hemophilosis accounted for > 40% of the mortality in feedlot calves each year. Haemophilus somnus was cultured from the blood of one febrile calf on day 1 (0.1%, n = 895), but it was not cultured from nasal swabs on day 1 or day 11 (n = 881) or from blood samples on day 11 (n = 883). Similarly, it was not cultured from nasal swabs or blood samples from sick calves first treated for bovine respiratory disease (BRD) (n = 219). Serological titers to H. somnus increased (p < 0.05) in unvaccinated calves from day 1 (Geometric mean titer = 11,846) to day 96 (Geometric mean titer = 63,712), indicating natural infection following feedlot entry. Calves that relapsed twice with BRD or died from BRD +/- hemophilosis had significantly (p < 0.06) lower titers to H. somnus on days 1 and 96 than those that did not relapse twice or die. Postarrival mass medication with long-acting oxytetracycline did not reduce (p > 0.05) the risk of hemophilosis mortality. However, it reduced (p < 0.05) the risk of BRD treatment by 14% and the risk of BRD mortality by 71%. Additional epidemiological studies of H. somnus are needed so that we can develop strategic medication and vaccination programs to reduce losses from hemophilosis. 相似文献
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White Emma L. Thomasson J. Alex Auvermann Brent Kitchen Newell R. Pierson Leland Sandy Porter Dana Baillie Craig Hamann Hendrik Hoogenboom Gerrit Janzen Todd Khosla Rajiv Lowenberg-DeBoer James McIntosh Matt Murray Seth Osborn Dave Shetty Ashoo Stevenson Craig Tevis Joe Werner Fletcher 《Precision Agriculture》2021,22(1):306-315
Precision Agriculture - Data-centric technology has not undergone widespread adoption in production agriculture but could address global needs for food security and farm profitability. Participants... 相似文献
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RE Hickson WJ Anderson PR Kenyon N Lopez-Villalobos ST Morris 《New Zealand veterinary journal》2013,61(4):176-183
AIM: To obtain an estimate of the incidence of assistance at calving in primiparous (first-calving) beef heifers and the prevalence of breeding heifers at 15 months of age in New Zealand in 2006,and to identify factors contributing to farmers' decisions regarding breeding strategies for heifers, using a survey of beef cattle farmers. METHODS: A questionnaire was sent to farmers listed in a Massey University database and to members of selected breed societies, as well as published in an industry newspaper; 331 valid responses were received. Information was gathered on the age and number of primiparous heifers, number of heifers assisted, and the importance of various reasons for and against breeding heifers at 15 months of age. Respondents were also required to outline the criteria used for selecting bulls to join with heifers, and the strategies used to manage dystocia in primiparous heifers. RESULTS: Sixty-five (95% CI=58–71)% of respondents had only 2-year-old primiparous heifers in 2006, whilst a further 11 (95% CI=8–16)% had both 2- and 3-year-old primiparous heifers. The mean reported incidence of assisted calving was 7.0 (95% CI=6.4–7.5)% for 2-year-old primiparous heifers and 1.7 (95% CI=1.2–2.2)% for 3-year-old primiparous heifers. The reported incidence of assistance at calving within individual herdsranged from 0 to 100% for 2-year-old heifers. Respondents with bull-breeding herds most commonly observed their primiparous 2-year-old heifers twice daily, whilst respondents with commercial herds most commonly observed them once daily during calving. The most important reason for breeding heifers at 15 months of age was “increased profit”, whereas the most important reason for not breeding them at that age was “concern about rebreeding performance of 2-year-old heifers”. Estimated breeding value (EBV) for birth weight was the factor considered most frequently when selecting bulls to join with maiden heifers; age of bull and body shape of bull were the next most frequently considered factors. Selection of an appropriate bull was the most common strategy used to manage dystocia in 2-year-old beef heifers. CONCLUSIONS: The majority of respondents calved their heifers at 2 years of age, and “increased profit” was the primary motivator. Concern about the rebreeding performance of 2-year-old heifers was the most important reason among the remainder of respondents for not breeding heifers at 15 months of age. Dystocia in 2-year-old heifers was “not a problem” or “a minorproblem” in most herds, but there was much variation amongst herds. 相似文献
70.
Recovery and dynamics of decomposing plant residue in soil: an evaluation of three fractionation methods 下载免费PDF全文
A. Diochon A. W. Gillespie B. H. Ellert H. H. Janzen E. G. Gregorich 《European Journal of Soil Science》2016,67(2):196-205
Our goals in this study were to track the incorporation of plant residue into soil organic matter (SOM) and test the effectiveness of different fractionation methods to evaluate this transformation. We incubated soil amended with 13C‐labelled barley (Hordeum vulgare L.) residue and used three fractionation methods based on size (> 250, 53–250, 5–53 and < 5 µm) and density (< 1.7 g cm?3, i.e. light fraction (LF)) and determined its quantity and the rate of C loss or gain or both in these fractions as decomposition progressed. One method was based on size only, another involved density separation followed by size fractionation and a third separated organic matter fractions by size first and then by density. There were significant quantitative differences between the methods for the amount of residue in the fractions, but there was no effect of fractionation method on the rate of change in the residue that comprised the fractions. The density method did not appear to identify all of the most recently added (i.e. least decomposed) residue in the LF or that there was a redistribution of SOM among the fractions. The amount of residue C and the C:N ratio of the residue in the two smallest fractions increased early during the incubation (0–2 months), but subsequently decreased towards the end. The initially small C:N ratio in the clay fraction probably reflects the accumulation of microbial by‐products from the rapid decomposition of water‐soluble compounds. The subsequent increase and decrease in both residue C and C:N ratio reflects the balance of the accumulation of sorbed water‐soluble compounds and dense plant residue fragments and their mineralization over time. We conclude that clay is a sink for residue C (i.e. microbial metabolites) early during decomposition, and that there is a transfer among fractions and mineralization of residue C as decomposition proceeds. These findings indicate that the clay fraction contains a dynamic pool of C that can cycle within short time‐scales. 相似文献