Methods of detection of Chlamydia psittaci in domesticated and wild birds     

CL McELNEA  GM CROSS 《Australian veterinary journal》1999,77(8):516-521

OBJECTIVE: To study the occurrence of Chlamydia psittaci in domesticated and wild birds and compare the sensitivity of molecular detection with cell culture isolation. DESIGN: Study of cell culture isolation and PCR detection of C psittaci in avian samples. PROCEDURE: Samples were obtained from 485 birds. Domesticated birds were selected at random from pet shops, private aviaries and zoos, while wild birds were captured locally, sampled, and immediately released. Swabs were collected from choanal slit, conjunctiva and cloaca of each bird and pooled. Samples were divided into equal portions for use in PCR dot-blot and cell culture detection. PCR and dot-blot detection was based on the ompB gene. RESULTS: Prevalence of infection varied markedly between flocks of captive birds. It was highest where there were frequent changes in the flock members or where there were many birds confined in small areas. C psittaci was not detected in wild birds or water birds. The sensitivity of cell culture compared to PCR dot-blot detection was 68%. All samples positive by cell culture were also positive by PCR. CONCLUSIONS: PCR-dot blot detection of C psittaci in birds appears to be more sensitive than cell culture isolation in this study. C psittaci infection of birds may occur in clinically normal captive birds.  相似文献   

Survey of captive parrot populations around Port Phillip Bay,Victoria, Australia,for psittacine beak and feather disease virus,avian polyomavirus and psittacine adenovirus          下载免费PDF全文

CL Hulbert  A Chamings  KA Hewson  PA Steer  M Gosbell  AH Noormohammadi 《Australian veterinary journal》2015,93(8):287-292

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Letter: Insulin activity: the solid matrix   总被引：3，自引：0，他引：3  

R W Butcher  O B Crofford  S Gammeltoff  J Gliemann  J R Gavin  I D Goldfine  C R Kahn  M Rodbell  J Roth  L Jarrett  J Larner  R J Lefkowitz  R Levine  G V Marinetti 《Science (New York, N.Y.)》1973,182(110):396-398

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14 EVALUATION OF BACTERIAL CONTAMINATION AFTER UNCOMPLICATED EQUINE CASTRATIONS.     

MJ  Schoonover  HD Moll  CL Crowson  HW Jann 《Veterinary surgery : VS》2003,32(6):601-602

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Prevalence of bovine immunodeficiency virus infection in cattle in Great Britain.     

L Scobie  C Venables  A R Sayers  S Weightman  O Jarrett 《The Veterinary record》2001,149(15):459-460

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Detection of transient and persistent feline leukaemia virus infections   总被引：3，自引：0，他引：3  

O Jarrett  M C Golder  M F Stewart 《The Veterinary record》1982,110(10):225-228

A study was made of cats persistently or transiently viraemic with feline leukaemia virus (FeLV) following experimental oronasal infection. Cats of two ages were exposed to the virus. One group was infected when eight weeks old in the expectation that most of the cats would become persistently viraemic, and the second group when 16 weeks old, so that some would show signs of a transient infection and then recover. The periods following infection when virus was detectable in the blood and in the oropharynx were determined for each group. Three methods for detecting viraemia were compared: virus isolation, immunofluorescence on blood smears and an enzyme-linked immunosorbent assay (ELISA). There was good overall agreement among the three tests in detecting virus-positive cats. Virus was found sooner after infection by virus isolation than by the other methods, and virus appeared in the blood slightly sooner in cats which developed persistent viraemia than in transiently viraemic cats. Infectious FeLV was isolated from the oropharynx of all of the persistently viraemic cats, in most cases simultaneously with virus in the plasma. Virus was also isolated from the mouth of most transiently viraemic cats. Under field conditions such transient excretion of virus lasting only a few days would rarely be detected in a single sampling. This might explain how FeLV is maintained in free range urban cats in the absence of a large number of cats with persistent active FeLV infection. For routine diagnosis, immunofluorescence would appear to offer the best chance of differentiating transient and persistent infections by FeLV.  相似文献   

A comparison of three methods of feline leukaemia virus diagnosis   总被引：5，自引：0，他引：5  

O Jarrett  M C Golder  K Weijer 《The Veterinary record》1982,110(14):325-328

Samples of blood from pet cats were examined for evidence of feline leukaemia virus (FeLV) by three techniques: virus isolation, immunofluorescence and an enzyme-linked immunosorbent assay (ELISA) Leukassay F. There was good agreement between the results from virus isolation and immunofluorescence. However, about 30 per cent of cats which were positive for FeLV antigen by ELISA were negative by either of the other tests. The status of most of these cats was unchanged four or 12 weeks later.  相似文献   

Monocytic leukaemia in a cow     

L J Mackey  W F Jarrett  A Wiseman 《Research in veterinary science》1972,13(3):287-289

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Duration of the latent state in feline leukaemia virus infections   总被引：2，自引：0，他引：2  

A M Pacitti  O Jarrett 《The Veterinary record》1985,117(18):472-474

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Transmission of feline immunodeficiency virus from mother to kitten.     

J J Callanan  M J Hosie  O Jarrett 《The Veterinary record》1991,128(14):332-333

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