Characterization of Amaranthus cruentus L. seed proteins by 2-DE and LC/MS–MS: Identification and cloning of a novel late embryogenesis-abundant protein     

Enrique Maldonado-Cervantes  José A. Huerta-Ocampo  Gabriela M. Montero-Morán  Alberto Barrera-Pacheco  Eduardo Espitia-Rangel  Ana P. Barba de la Rosa 《Journal of Cereal Science》2014

Amaranth is taking great attention as an important cereal crop that could fulfill food requirements for the growing population, especially in developing countries. However, the protein composition of these seeds is not well known yet. We have used the proteomics tools to characterize amaranth seed proteome. About 400 proteins spots were resolved on two-dimensional gel electrophoresis (2-DE) and protein spots were analyzed by LC-MS/MS (liquid chromatography tandem mass spectrometry). Identified proteins were related to stress and defense responses, metabolic, respiratory, and oxide-reduction processes. One abundant spot was identified as a Late Embryogenesis Abundant (LEA) protein and the gene was cloned and characterized. The AcLEA cDNA contains a 418 bp open reading frame (ORF) encoding a polypeptide of 139 amino acids. Bioinformatics analysis showed that AcLEA belongs to LEAs Group 5. Proteomics is a powerful technique that could be used even in non-sequenced organisms such as amaranth. The obtained information reveals that amaranth seed, beyond the classical seed storage proteins, contains proteins related to protection against stress. The identification of these proteins opens the door to the application of new strategies to improve the quality of amaranth production.  相似文献   

Biosynthetic Modularity Rules in the Bisintercalator Family of Antitumor Compounds     

Javier Fernández  Laura Marín  Raquel álvarez-Alonso  Saúl Redondo  Juan Carvajal  Germán Villamizar  Claudio J. Villar  Felipe Lombó 《Marine drugs》2014,12(5):2668-2699

Diverse actinomycetes produce a family of structurally and biosynthetically related non-ribosomal peptide compounds which belong to the chromodepsipeptide family. These compounds act as bisintercalators into the DNA helix. They give rise to antitumor, antiparasitic, antibacterial and antiviral bioactivities. These compounds show a high degree of conserved modularity (chromophores, number and type of amino acids). This modularity and their high sequence similarities at the genetic level imply a common biosynthetic origin for these pathways. Here, we describe insights about rules governing this modular biosynthesis, taking advantage of the fact that nowadays five of these gene clusters have been made public (thiocoraline, triostin, SW-163 and echinomycin/quinomycin). This modularity has potential application for designing and producing novel genetic engineered derivatives, as well as for developing new chemical synthesis strategies. These would facilitate their clinical development.  相似文献   

How well does a botanical garden collection of a rare palm capture the genetic variation in a wild population?   总被引：1，自引：0，他引：1  

Sandra Namoff  Javier Francisco-Ortega  Larry R. Noblick  M. Patrick Griffith 《Biological conservation》2010,143(5):1110-1117

Conservation is increasingly central to the botanic garden mission. Living plant collections are important components of conservation. Critical evaluation of living conservation collections with population genetic analysis can directly inform ex situ conservation strategy. Here, we quantify the degree of genetic variation captured through a population-based collection protocol, and explore optimal sampling for ex situ conservation. An extensive living collection derived from one population of Leucothrinax morrisii (Arecaceae) provided a model system. We compared 58 specimens from the ex situ collection with 100 individuals from throughout the parent population via 6 ISSR loci. Random bootstrapped resamples of the data were made to model differently structured ex situ collections. Mean diversity (He) differed little between the collection (0.204) and the population (0.216), and genetic distance (D) was very close (0.036). Very few private alleles were found between the collection and the population. Allelic capture, as measured by percent of private alleles, was greater than 94%. Resampled collections of different sizes captured from 48% to 94% of alleles. Pairwise comparison of bootstrapped resamples suggests that increasing the representation of half-sibling groups does not significantly increase allele capture. Increase in allele capture with increasing sample size is greatest at low resample sizes, and showed diminishing returns as resample size increased. No appreciable increase in allele capture was gained through maintaining different half-sibling groups. These data inform sampling for ex situ conservation purposes, and recommend sample sizes of at least 15 individuals, with the upper limit based on resources.  相似文献   

Drying-rewetting cycles and γ-irradiation effects on enzyme activities of distinct layers from a Quercus ilex L. litter     

Enrique Alarcón-Gutiérrez  Fabio Ziarelli  Stéven Criquet 《Soil biology & biochemistry》2010,42(2):283-290

In a Mediterranean climate, water stress is one of the principal constraints on proper forest ecosystem functioning. Drought influences rates of organic matter degradation by affecting microbial growth and enzyme activities. The objectives of this study were: (i) to evaluate the effect of repeated drying-rewetting cycles on cellulase, alkaline phosphatase and fluorescein diacetate (FDA) hydrolase activities of three distinct Quercus ilex L. litter layers, and (ii) to investigate the effect of these cycles on γ-irradiated litters in order to distinguish the abiotic influence on the fluctuations observed. Results, for all three layers, showed high correlations between litter water content and enzyme activities. Under mesocosm conditions, and using non-sterilized litter samples, cellulase, alkaline phosphatase, and FDA activities significantly decreased or increased during drying or rewetting cycles respectively. Significant differences were also found when evaluating the effect of litter depth on enzyme activities, the intermediate depth (OLv layer) generally being the most active. For γ-sterilized samples, FDA activity still fluctuated with drying-rewetting cycles. Assays showed that pre-humidification of γ-irradiated litter increased FDA activity two-fold in the first 30 min. All these results have shown that, following drying-rewetting cycles, some of the fluctuations occur independently of microbial growth, suggesting abiotic interactions, such as desorption, in combination with both solvatation status and conformational changes of enzymes.  相似文献   

A Modeling Study of the Impact of a Power Plant on Ground-Level Ozone in Relation to its Location: Southwestern Spain as a Case Study     

Nuria Castell  Enrique Mantilla  Ariel F. Stein  Rosa Salvador  Millán Millán 《Water, air, and soil pollution》2010,209(1-4):61-79

The impact of atmospheric industrial emissions on secondary pollutant formation depends on many factors; one of the most important being the environmental setting in which the industry is located. The environmental setting affects an industry’s impact on ozone levels through both the air mass dispersion (a function of topography and wind patterns) and the emissions of organic volatile compounds (VOC) and nitrogen oxides (NO _x ) in the area. This model-based study shows how the sensitivity of surface ozone changes with the choice of source location. For the analysis, seven points distributed along the Tinto–Guadalquivir Basin (in the Southwestern Iberian Peninsula) were selected. This area is characterized by the close proximity of natural environments and crop fields to cities, roads, and industrial areas with high NO _x emissions. Natural VOC emissions represent more than 60% of the total non-methane volatile organic compounds emitted in the study area. The results reveal that the largest increases in ozone levels are produced when the industry is located both far away from NO _x emission sources and near to biogenic VOC emissions. Furthermore, the highest increases over the hourly and 8-hourly maximums, as well as the highest accumulated daily values, are found in areas characterized by high VOC/NO _x emission ratios and NO _x sensitivity. The study of the recurrent meteorological patterns along with the distribution of chemical indicators of the O₃–NO _x –VOC sensitivity allows the determination of the industry’s geographical impact on ozone levels. This information enables air quality managers to decide the future location of an industry minimizing its impact on smog levels.  相似文献   

商用鸡免疫系统的评估     

Enrique Montiel 《中国家禽》2002,24(2):10-13

健康的鸡只在正常情况下会有良好的生产性能表现.但是,要养好健康的鸡群,则有赖于许多因素.这些因素包括管理、营养、免疫计划,而最重要的是有赖于免疫系统的健全发育.免疫系统的发育会受到数种病毒及非病毒因素的影响.在病毒方面,传染性法氏囊病病毒(IBDV)、马立克氏病病毒(MDV)、里奥病毒(REO)、鸡传染性贫血病病毒(CAV)、禽白血病病毒(ALV)等数种为引起免疫抑制的主要病毒.  相似文献   

Factorial regression analysis of genotype by environment interaction in ryegrass populations, using both isozyme and climatic data as covariates     

François Balfourier  Jose Alberto Oliveira  Gilles Charmet  Enrique Arbones 《Euphytica》1997,98(1-2):37-46

A sample of 58 natural ryegrass populations (Lolium perenne L.) from the French and Spanish oceanic coast was evaluated for three seasonal growth traits (i.e. spring, autumn and winter vigour traits) in 5 evaluation sites (three in France and two in Spain). This sample showed a high level of genotype (population) × environment (evaluation sites) interactions for the three agronomic traits. A factor regression analysis, using both isozyme frequencies of populations and climatic factors of evaluation sites as covariates, was carried out on a sub-sample of 30 populations in order to explain these interactions. This method succeeded in explaining most population × location interactions by the product of two covariates. For instance, for spring vigour trait, 72.8% of the interaction term could be explained by the use of two covariates: the PGI2-20 allelic frequency of populations and the minimum temperature of the coldest month of evaluation sites. This study shows the advantage of such a method for plant breeders who need to identify promising ryegrass populations for their breeding objectives. A number of genetic and evolutionary hypotheses are also discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Lipid composition of lees from Sherry wine     

Gómez ME  Igartuburu JM  Pando E  Luis FR  Mourente G 《Journal of agricultural and food chemistry》2004,52(15):4791-4794

In this paper, we describe the study and characterization of the lipids from lees of Sherry wine, one of the main byproducts from the wine-making industry in the Jerez/Xeres/Sherry denomination of the origin zone in Jerez de la Frontera, Spain. The lipid content, extractability, classification, fatty acid composition, and its main chemical characteristics have been determined in order to evaluate their potential use as a food or food additive.  相似文献   

Embolism induced by winter drought may be critical for the survival of <Emphasis Type="Italic">Pinus sylvestris</Emphasis> L. near its southern distribution limit     

José Javier Peguero-Pina  José María Alquézar-Alquézar  Stefan Mayr  Hervé Cochard  Eustaquio Gil-Pelegrín 《Annals of Forest Science》2011,68(3):565-574

Introduction   

Scots pine populations in the SE “Sistema Ibérico” range suffered a severe defoliation in the S face of the crown in isolated trees of thinned stands. This process was detected at the end of the winter 2001–2002.  相似文献   

Estimating balanced structure areas in multi-species forests on the Sierra Madre Occidental,Mexico   总被引：1，自引：0，他引：1  

Christian Wehenkel  José Javier Corral-Rivas  José Ciro Hernández-Díaz  Klaus von Gadow 《Annals of Forest Science》2011,68(2):385-394

• Introduction   

This study presents a method for estimating the minimum area which exhibits a balanced diameter distribution, and the corresponding number of trees, for different tree species and forest types in the Santiago Papasquiaro region in the State of Durango, Mexico. The balanced structure area is defined as the minimum contiguous area that is required for sustainable management of a multi-sized selection forest. A multi-sized forest represents a balanced structure unit if the relationship between harvest and growth can be maintained, using a defined target diameter distribution and disregarding major natural disturbances. The study is based on 17,577 sample plots in uneven-aged forests, which are selectively harvested by local communities.  相似文献