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Background

Application of assisted reproductive technologies in buffaloes is limited to some extent by farmers’ inability to detect oestrus because of its poor expression. The present study aimed at investigating reliability of a milk progesterone enzyme-linked immunosorbent assay (ELISA) to assess the ovarian cyclicity during post partum, oestrus and post-breeding periods in water buffaloes.

Methods

Progesterone concentrations were measured by an ELISA in milk of 23 postpartum buffaloes in relation to oestrus, pregnancy, body condition score (BCS) and milk production. Two milk samples were taken at 10 days intervals, every month starting from day 30 and continued to day 150 post partum. BCS and milk production were recorded during sample collection. Milk samples from bred buffaloes were collected at Day 0 (day of breeding), Days 10–12 and Days 22–24. Defatted milk was preserved at −80°C until analysis. Pregnancy was confirmed by palpation per rectum on Days 70–90.

Results

Seventeen buffaloes had 47 ovulatory cycles, one to four in each, 13 were detected in oestrus once (28 % oestrus detection rate). Progesterone concentration ≥1 ng/ml in one of the two 10-day-interval milk samples reflected ovulation and corpus luteum formation. The intervals between calving to first luteal activity and to first detected oestrus varied from 41 to 123 days (n = 17) and 83 to 135 (n = 13) days, respectively. Eight buffaloes were bred in the course of the study and seven were found pregnant. These buffaloes had a progesterone profile of low (<1 ng/ml), high (≥ 1 ng/ml) and high (≥ 1 ng/ml) on Day 0, Days 10–12 and Days 22–24, respectively. Buffaloes cycling later in the postpartum period had fewer missed oestruses (P < 0.05). Buffaloes with a superior BCS had a shorter calving to oestrus interval and produced more milk (P < 0.05).

Conclusions

Milk progesterone ELISA is a reliable tool for monitoring ovarian cyclicity and good BCS may be an indicator of resuming cyclicity in water buffalo.  相似文献   
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European Journal of Plant Pathology - Plant viruses are the most serious pathogens causing severe damage to crops worldwide. A variety of conventional strategies are currently being employed to...  相似文献   
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Field experiments were conducted at Agricultural Experimental Farm, Giridih, Jharkhand, during the kharif (wet) seasons of consecutive four years (1998–2001) to study the effect of staggered seeding of blackgram (Phaseolus mungo L.) in rice field as a cereal–legume intercropping system and to minimize the degree of competition between the crop species to avoid yield damage, and at the same time to create a high level of competition by the intercrops to suppress the weeds. Intercropping systems were assessed on the basis of land equivalent ratio, relative crowding coefficient, aggressivity, actual yield loss, monetary advantage, etc. Intercropping reduced the yield of component crops when compared with respective pure stands. However, deferred seeding of blackgram in rice (30 cm) after one weeding was most remunerative system and registered maximum rice-equivalent yield (2711 kg ha−1). Rice–blackgram (20 cm) intercropping system was very effective for weed smothering among unweeded intercropping treatments. In conclusion, deferred seeding of blackgram in rice field (30 cm) with one weeding may be recommended for better yield, weed suppression and better economics in the eastern plateau region of India.  相似文献   
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