Simultaneous quantification of the relative abundance of species complex members: application to Culicoides obsoletus and Culicoides scoticus (Diptera: Ceratopogonidae), potential vectors of bluetongue virus     

Mathieu B  Delecolle JC  Garros C  Balenghien T  Setier-Rio ML  Candolfi E  Cêtre-Sossah C 《Veterinary parasitology》2011,182(2-4):297-306

The two sympatric sibling species Culicoides obsoletus (Meigen) and Culicoides scoticus Downes and Kettle (Diptera: Ceratopogonidae), are known to be competent vectors for bluetongue virus in the Palaearctic region. However, morphological identification of constituent species is only readily applicable to adult males and these two species distinguishing traits have overlapping character states. As their vector competence may differ in space and time, the correct identification and quantification of specimens of both species are essential for understanding bluetongue epidemiology. However, no molecular tools are available for high-throughput identification of the two species. We therefore developed a quantitative duplex real-time PCR assay to determine the relative abundance of each sibling species in a sample using TaqMan probes. For each species, standard curves were constructed from serial dilutions of purified plasmid DNA containing ITS1-5.8S-ITS2 (rDNA) in the range of 10(-1) to 10(-5)ng/μL. Standard curves were used to quantify samples of mixed C. obsoletus/C. scoticus specimens. Specificity was evaluated with 5156 specimens representing 62 species. Based on the DNA quantities detected according to the standard curves, a quadratic model developed on 1100 males and validated on 555 females was able to predict the relative abundance of each species simultaneously in a one-shot reaction (Pearson coefficient of 0.999). Our assay showed a requirement of two specimens or less for 95% of the predictions, making it highly applicable to field collections. Extensive use of this real-time PCR assay will provide a better understanding of geographical distribution, dynamics, and bionomics on a species level, which is essential for risk assessment. This approach is an important contribution to medical entomology for investigating the vector role of arthropod sibling species.  相似文献   

31P NMR characterization and efficiency of new types of water-insoluble phosphate fertilizers to supply plant-available phosphorus in diverse soil types     

Erro J  Baigorri R  Yvin JC  Garcia-Mina JM 《Journal of agricultural and food chemistry》2011,59(5):1900-1908

Hydroponic plant experiments demonstrated the efficiency of a type of humic acid-based water-insoluble phosphate fertilizers, named rhizosphere controlled fertilizers (RCF), to supply available phosphorus (P) to different plant species. This effect was well correlated to the root release of specific organic acids. In this context, the aims of this study are (i) to study the chemical nature of RCF using solid-state (31)P NMR and (ii) to evaluate the real efficiency of RCF matrix as a source of P for wheat plants cultivated in an alkaline and acid soil in comparison with traditional water-soluble (simple superphosphate, SSP) and water-insoluble (dicalcium phosphate, DCP) P fertilizers. The (31)P NMR study revealed the formation of multimetal (double and triple, MgZn and/or MgZnCa) phosphates associated with chelating groups of the humic acid through the formation of metal bridges. With regard to P fertilizer efficiency, the results obtained show that the RCF matrix produced higher plant yields than SSP in both types of soil, with DCP and the water-insoluble fraction from the RCF matrix (WI) exhibiting the best results in the alkaline soil. By contrast, in the acid soil, DCP showed very low efficiency, WI performed on a par with SSP, and RCF exhibited the highest efficiency, thus suggesting a protector effect of humic acid from soil fixation.  相似文献   

The effects of densities and feed types on the production of Macrobrachium rosenbergii (de Man) in the rotational rice–prawn system     

Lam My Lan  Duong Nhut Long  & Jean-Claude Micha 《Aquaculture Research》2006,37(13):1297-1304

Postlarvae (PL) of Macrobrachium rosenbergii (de Man 1879) were stocked at 1, 2, 3 and 4 PL m^?2. Prawns were fed only pellets (P) and a combination of pellets and snail meat (PS) with three replications in 100 m² plots in the rotational prawn–rice system. Water levels were 0.3–0.6 m in rice growing areas. The water temperatures at noon in the hot months were higher than the suitable range for prawns. The final mean weights of prawns in treatments 1, 2 and 3 PL m^?2 were significantly higher than in treatment 4 PL m^?2 (P<0.05). The yields from different density treatments ranged from 194±82 to 373±32 kg ha^?1 and increased significantly with the increases in prawn densities (P<0.05). There was no significant difference between the two types of feed on production parameters (P>0.05). The total cost significantly increased at higher densities (P<0.05), but it was not different between feed types (P>0.05). Net profit and cost benefit ratio of treatment PS were significantly higher than in treatment P. In the present study, treatment 3 PL m^?2 and treatment PS offered the optimal results.  相似文献   

Effect of the use of dilute alkaline prior to Bacillus subtilis-based biocontrol steeping and germination conditions on red sorghum malt β-glucanase activities and residual β-glucans     

Jean-Claude Bwanganga Tawaba  Jacqueline Destain  Paul Malumba Kamba  François Béra  Philippe Thonart 《Journal of Cereal Science》2013

Malting is the ideal stage to deal with β-glucans. Their hydrolysis is very important as the diffusion of both hormones and hydrolytic enzymes in the endosperm of germinated grain depend on it. A high malt β-glucanase activity is not a guarantee of an extensive hydrolysis of β-glucans. When Bacillus subtilis is used to control mould growth, red sorghum malt β-glucanase activity (measured using carboxymethylcellulose as the substrate) was improved without significantly affecting the hydrolysis of malt β-glucans. Thus, in order to reduce the residual β-glucans content, soaking in 0.2% NaOH was combined with a biocontrol. Soaking in 0.2% NaOH is recognized as capable of improving grain hydration by opening-up the endosperm cell walls. The combined use of 0.2% NaOH with B. subtilis-based biocontrol treatments during red sorghum malting, leads to malt with increased β-glucanase activity and a significant reduction of residual β-glucans when compared with the 16 h biocontrol steeping without prior steeping in 0.2% NaOH. β-glucanase activity increases with increased germination temperature and time while, conversely, the residual β-glucans content of the malts decreases. Indeed, while the level of β-glucanase was not vastly different between the malts obtained after steeping in distilled water and those obtained after 8 h steeping in 0.2% NaOH followed by 8 h resteeping in distilled water (NaOH + H₂O treatment), their residual β-glucans levels differ significantly. B. subtilis-based treatment leads to malt with improved β-(1-3)- and β-(1-4)-glucanase activities without significantly improved malt β-(1-3),(1-4)-glucanase activity. While malts obtained after 84 h germination weren't significantly different in terms of malt β-(1-3),(1-4)-glucanase activities for all steeping treatments, the use of 0.2% NaOH steeping prior to resteeping led to malts with improved β-glucans content. Combining the steeping in dilute alkaline and biocontrol enables taking advantage of the dilute alkaline effect on residual β-glucans content, due probably to the opening-up of the cell walls and the improvement of water uptake, and that of the biocontrol (improvement of β-glucanase synthesis).  相似文献   

Suppressing weeds in direct-seeded lowland rainfed rice: Effect of cutting dates and timing of fertilizer application     

Suchada Sanusan  Anan Polthanee  Alain Audebert  Surasak Seripong  Jean-Claude Mouret 《Crop Protection》2010

Weeds cause substantive yield losses in rainfed rice, particularly direct-seeded rice (DSR). Two field experiments were conducted in 2005 in north-east Thailand to examine the effects of cutting date and timing of fertilizer application on suppressed weed, growth and yield of DSR. Treatments consisted of two fertilizer applications to the main plots (top-dressing at the date of cutting and 15 days after cutting; DAC) and four cutting dates of the sub-plots. Rice and weed cutting was performed at 30, 45, 60 and 75 days after seeding (DAS), compared with an untreated control. Cutting resulted in a higher growth rate and improved grain yield of rice compared to the untreated control. Weed cutting at 60 DAS resulted in the greatest leaf area index, which contributed to the maximum number of tiller as well as maximum plant dry mass when observed at 30 DAC. Lower densities and dry weights of weeds at 30 DAC were observed when cutting was done 60 DAS, resulting in a higher grain yield than the other cutting dates. Timing of fertilizer application had no significant effect on the density and dry weight of grasses and broad-leaved weeds, but did have a significant affected on, resulting in the lowest density of sedges at 45 DAC was observed with fertilizer applied at cutting date. Rice grain yield was higher with top-dressing of fertilizer at 15 DAC than with fertilizer applied at cutting date. The cutting treatments resulted in higher net incomes than the untreated control. These results indicate that rice and weed cutting at an appropriate growth stage is an alternative method to chemical weed control for DSR in rainfed riceland.  相似文献   

Morphogenetic analysis of the phenotypic variability of the architectural unit of Hydrangea macrophylla     

Gilles Galopin  Jean-Claude Mauget  Philippe Morel 《Annals of Forest Science》2010,67(3):309-309

  相似文献   

Investigating the possible impact of atmospheric CO2 increase on Araucaria araucana wood density     

Paulina E. Pinto  Jean-Daniel Bontemps  Jean-Claude Pierrat  Tony Franceschini  Pierre Gelhaye  Jean-Claude Gégout  J. M. Leban 《European Journal of Forest Research》2016,135(2):389-401

  相似文献   

Evaluation of beta3-adrenoceptor-mediated relaxation in intact and endotoxin-treated equine digital veins   总被引：1，自引：0，他引：1  

Mallem MY  Gogny M  Gautier F  Bucas V  Desfontis JC 《American journal of veterinary research》2003,64(6):708-714

OBJECTIVE: To investigate the functional expression of beta3-adrenoceptors (beta3-ARs) in equine digital veins (EDVs) and to examine whether beta3-AR relaxation was altered in EDVs incubated with endotoxin. SAMPLE POPULATION: Forelimbs obtained from 30 horses. PROCEDURE: Forelimbs were obtained from horses in an abattoir. Equine digital veins were carefully removed from distal portions of the forelimbs. Rings of dissected EDVs were mounted in 5-mL organ baths to record isometric tension in the presence of various beta3-AR agonists (SR 58611A, ZD 2079, and ZM 215001). RESULTS: In intact EDVs, isoprenaline, SR 58611A, ZD 2079, and ZM 215001 induced concentration-dependent relaxation. Isoprenaline and SR 58611A-induced relaxations were reduced or unaffected by nadolol, respectively. In intact EDVs, SR 58611A-induced relaxation was significantly reduced in the presence of 2 microM ZM 215001 (used as a beta3-AR antagonist). In endothelium-denuded EDVs or intact EDVs in the presence of a nitric oxide synthase inhibitor, isoprenaline and SR 58611A-induced relaxations were significantly decreased. The endothelium-independent relaxation to SR 58611A was significantly inhibited in the presence of ZM 215001. In endotoxin-treated EDV, isoprenaline- and SR 58611A-induced relaxations were significantly reduced. In these conditions, cycloheximide (a protein synthesis inhibitor) and ibuprofen (a cyclooxygenase inhibitor) restored the relaxant response to SR 58611A. CONCLUSIONS AND CLINICAL RELEVANCE: Beta3-adrenoceptors are functionally expressed in EDVs. Incubation in the presence of endotoxin, used as an in vitro model of laminitis, induced an alteration of beta-AR-mediated relaxations in EDVs, which could be the consequence of cyclooxygenase induction and subsequent prostanoid production.  相似文献   

Spatial Distribution of Ambrosia-Beetle Catches: A Possibly Useful Knowledge to Improve Mass-Trapping     

Jean-Claude Grégoire  Frédéric Piel  Michel De Proft  Marius Gilbert 《Integrated Pest Management Reviews》2001,6(3-4):237-242

Several species of ambrosia beetles (Coleoptera: Scolytidae) have recently started attacking standing, living beeches (Fagus sylvatica) in southern Belgium. In 2001, 1.3 million m³ of apparently healthy trees were struck. So far the outbreak has been limited to the Ardenne, and partly the Gaume, areas, and Brussels has been untouched. The city of Brussels is surrounded by a vast 4300 ha forest, mainly planted with beech, the Forêt de Soignes, of invaluable ecological and recreational value, of which 1600 ha belong to the regional authorities. In the spring 2001, these latter commissionned a study to assess the new threat to the forest. A 500 m×500 m grid of small traps, baited with ethanol and lineatin, was deployed over the regional part of the forest. The main species caught were Trypodendron domesticum, Anisandrus dispar and, in high numbers, Xylosandrus germanus, an exotic species of Asian origin found for the first time in Belgium in 1994. Whilst there was a consistent homogeneity between catches within the same sites (2 traps/site, distant by 2–6 m), there were no spatial relationships between catches at larger distances for T. domesticum and A. dispar. For X. germanus, spatial autocorrelations were observed within distances of 2000 m, suggesting that this species has sufficient mobility to cover this range. The planning of the 2002 trapping campaign will take this information into account: the traps will be deployed within a smaller grid.  相似文献   

Multiscale computation of solar radiation for predictive vegetation modelling     

Christian Piedallu  Jean-Claude Gégout 《Annals of Forest Science》2007,64(8):899-909

The recent development of large environmental databases allow the analysis of the ecological behaviour of species or communities over large territories. Solar radiation is a fundamental component of ecological processes, but is poorly used at this scale due to the lack of available data. Here we present a GIS program allowing to calculate solar radiation as well locally as at large scale, taking into account both topographical (slope, aspect, altitude, shadowing) and global (cloudiness and latitude) parameters. This model was applied to the whole of France (540 000 km²) for each month of the year, using only a 50-m digital elevation model (DEM), latitude values and cloudiness data. Solar radiation measured from 88 meteorological stations used for validation indicated a R ² of 0.78 between measured and predicted annual radiation with better predictions for winter than for summer. Radiation values increase with altitude, and with slope for southern exposure, excepted in summer. They decrease with latitude, nebulosity, and slope for north, east, and west exposures. The effect of cloudiness is important, and reduces radiation by around 20% in winter and 10% in summer. Models of plant distribution were calculated for Abies alba, Acer pseudoplatanus, and Quercus pubescens, for France. The use of solar radiation improved modelling for the three species models directly or through the water balance variable. We conclude that models which incorporates both topographical and global variability of solar radiation can improve efficiency of large-scale models of plant distribution.  相似文献