Seasonal Variation of Mercury Associated with Different Phytoplankton Size Fractions in Lahontan Reservoir, Nevada     

Rosemary W. H. Carroll  Jeramie Memmott  John J. Warwick  Christian H. Fritsen  Jean-Claude J. Bonzongo  Kumud Acharya 《Water, air, and soil pollution》2011,217(1-4):221-232

Sampling is conducted during 2006 in Lahontan Reservoir, Nevada to investigate seasonal variation of total mercury (THg) and methylmercury (MeHg) partitioning in different phytoplankton size fractions as a function of point source (fluvial) mercury (Hg) loads, reservoir residence time, and algal growth. Carson River Hg inputs into the reservoir are extremely dynamic with spring loads two orders of magnitude larger than summer loads. Chlorophyll a measurements show two periods of algal growth. A small amount of algal growth occurs March to May. A second more substantial bloom occurs in the late summer, which is dominated by large, filamentous algae. THg concentrations (C _b) and partitioning coefficients (K _d) in total suspended particulate matter (SPM) are highest when fluvial inputs of Hg-contaminated sediment are large and are not necessarily associated with living biomass. However, MeHg K _d in the small size fraction is indirectly related to fluvial loads and more strongly associated with living biomass in the later portion of the summer when algal growth occurs and reservoir residence times are longer. Data suggest size distinction is important to MeHg partitioning in the reservoir. Lumping all sizes into a single SPM sample will bias the analysis toward low MeHg C _b and low MeHg K _d in late summer when Aphanizomenon flos-aquae dominates the phytoplankton assemblage.  相似文献   

Effect of temperature and oxygen on the activity of microsomal oleyl-coenzyme A desaturase of potato tubers     

Abdelkader Cherif  Jean-Claude Kader 《Potato Research》1976,19(1):21-26

Summary To check if the oxygen concentration in the cell medium-higher at low temperatures-is a factor regulating the synthesis of polyunsaturated fatty acids, the effects of temperature and oxygen concentration were studied in vitro on the activity of the microsomal oleyl-coenzyme A desaturase of potato tubers. The highest activity of this enzyme was observed at 30° C, and not at low temperatures, and at an oxygen concentration of about 20%. These results suggest that the above hypothesis is not valid for the potato tuber cell.

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Zusammenfassung Um zu prüfen. ob die Sauerstoffkonzentration in der Zelle-bei niedriger Temperatur h?herein regulierender Faktor bei der Synthese der mehrfach unges?ttigten Fetts?uren ist, wurde der Einfluss der Temperatur und der Sauerstoffkonzentration auf die Aktivit?t der mikrosomalen Oleyl-coenzym A Desaturase der Kartoffelknollen in vitro untersucht. Die h?chste Aktivit?t des Enzyms wurde bei einer Temperatur von 30°C gefunden und nicht bei tieferen Temperaturen (Abb. 1) und bei einer Konzentration von ca. 20% Sauerstoff (Abb. 2). Diese Ergebnisse zeigen, dass die angeführte Hypothese im Falle der Kartoffelknollen nicht zutrifft.

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Résumé Pour vérifier si l'hypothèse selon laquelle la concentration en oxygène du milieu cellulaire-plus élevée à basse température-est un facteur régulant la synthèse des acides gras polyinsaturés, les effets de la température et de la concentration en oxygène sur l'activité de l'oléyl-coenzyme A désaturase microsomale du tubercule de pomme de terre ont été étudiés in vitro. L'activité la plus élevée de l'enzyme est observée pour la température de 30°C et non à basse température (Fig. 1) et pour une concentration en oxygène d'environ 20% (Fig. 2). Ces résultats suggèrent que l'hypothèse exposée plus haut n'est pas valable dans le cas du tubercule de pomme de terre.

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Diesel oil consumption,work duration,and crop production of corn and durum wheat under conventional and no-tillage in southeastern France     

Mohammadreza Khaledian  Jean-Claude Mailhol  Pierre Ruelle 《Archives of Agronomy and Soil Science》2013,59(8):1067-1076

In no-tillage (NT) system, precedent crop residue retains on the soil surface to preserve soil water for crop growth. In response to the negative impact of soil degradation under conventional tillage (CT) system based on soil tillage, NT system without tillage practice and with protective cover of crop residue is being developed in many parts of the world. However, NT is a successful system especially in the South of America, but the impacts of this system on the Mediterranean climate especially in the southeast (SE) of France is less known; therefore, this study has been carried out within the scope of a European project. Durum wheat and corn were sown under CT and NT. Time requirement and fuel consumption in these two systems were measured. The results showed that durum wheat and corn yields were the same in both systems except of 2008, while work duration and energy requirement were 87% and 83% lower in NT system, respectively. Furthermore, NT could mitigate CO₂ emission up to 50% as compared with CT. These results show that NT can be considered as a relevant alternative for CT regarding economical and environmental advantages.  相似文献   

Two new benzylbenzoate glucosides from Curculigo orchioides   总被引：2，自引：0，他引：2  

Valls J  Richard T  Larronde F  Leblais V  Muller B  Delaunay JC  Monti JP  Ramawat KG  Mérillon JM 《Fitoterapia》2006,77(6):416-419

An extract from in vitro cultures of Curculigo orchioides grown as bulbils in shake flasks, afforded two new glucosides of substituted benzylbenzoate - curculigoside C (3) and curculigoside D (4) - together with two known compounds - curculigoside A (1) and curculigoside B (2). Their structures were elucidated on the basis of spectral evidence, in particular by using 2D NMR methods. Their vasoactive properties were assessed in isolated rat aortic rings.  相似文献   

Disposition and metabolic profiling of bisphenol F in pregnant and nonpregnant rats     

Cabaton N  Chagnon MC  Lhuguenot JC  Cravedi JP  Zalko D 《Journal of agricultural and food chemistry》2006,54(26):10307-10314

The distribution of bisphenol F (4,4'-dihydroxydiphenyl-methane, BPF) was studied in female Sprague-Dawley rats. Pregnant and nonpregnant animals were gavaged with a single dose of 7 or 100 mg/kg [3H]BPF and were kept for 96 h in metabolic cages. The excretion of BPF residues occurred mainly in urine (43-54% of the administered dose), which was found to contain at least six different metabolites, and to a lesser extent in feces (15-20% of the administered dose). Sulfatase treatment and subsequent high-performance liquid chromatography analyses suggest that the major urinary metabolite (more than 50% of the radioactivity present in urine) is a sulfate conjugate of BPF. At 96 h, BPF residues were detectable in all tissues examined with the largest amounts in the liver (0.5% of the dose). In pregnant rats dosed at day 17 of gestation, BPF residues were detected in the uterus, placenta, amniotic fluid, and fetuses (0.9-1.3% of the administered dose). Large amounts of radioactivity (8-10% of the dose) were still located in the digestive tract lumen at the end of the study. After administration of a single oral dose of [3H]BPF, 46% of the distributed radioactivity was excreted in bile over a 6 h period. In rats, BPF and/or its metabolites very likely undergo enterohepatic cycling, which could be responsible for the relatively high amounts of residues still excreted 4 days after BPF administration. This bisphenol is efficiently absorbed and distributed to the reproductive tract in female rats, and its residues pass the placental barrier at a late stage of gestation in rats.  相似文献   

Bartonella and Rickettsia in arthropods from the Lao PDR and from Borneo, Malaysia     

Kernif T  Socolovschi C  Wells K  Lakim MB  Inthalad S  Slesak G  Boudebouch N  Beaucournu JC  Newton PN  Raoult D  Parola P 《Comparative immunology, microbiology and infectious diseases》2012,35(1):51-57

Rickettsioses and bartonelloses are arthropod-borne diseases of mammals with widespread geographical distributions. Yet their occurrence in specific regions, their association with different vectors and hosts and the infection rate of arthropod-vectors with these agents remain poorly studied in South-east Asia. We conducted entomological field surveys in the Lao PDR (Laos) and Borneo, Malaysia by surveying fleas, ticks, and lice from domestic dogs and collected additional samples from domestic cows and pigs in Laos. Rickettsia felis was detected by real-time PCR with similar overall flea infection rate in Laos (76.6%, 69/90) and Borneo (74.4%, 268/360). Both of the encountered flea vectors Ctenocephalides orientis and Ctenocephalides felis felis were infected with R. felis. The degrees of similarity of partial gltA and ompA genes with recognized species indicate the rickettsia detected in two Boophilus spp. ticks collected from a cow in Laos may be a new species. Isolation and further characterization will be necessary to specify it as a new species. Bartonella clarridgeiae was detected in 3/90 (3.3%) and 2/360 (0.6%) of examined fleas from Laos and Borneo, respectively. Two fleas collected in Laos and one flea collected in Borneo were co-infected with both R. felis and B. clarridgeiae. Further investigations are needed in order to isolate these agents and to determine their epidemiology and aetiological role in unknown fever in patients from these areas.  相似文献   

A simple stem taper model with mixed effects for boreal black spruce     

Geneviève Lejeune  Chhun-Huor Ung  Mathieu Fortin  Xiao Jing Guo  Marie-Claude Lambert  Jean-Claude Ruel 《European Journal of Forest Research》2009,128(5):505-513

We simplified Kozak’s taper model by setting the inflection point at 1.3 m (dbh) without losing accuracy and precision. The simplification was required to facilitate the estimation of the covariance parameters when using a mixed-effects method. This method was necessary to take into account the correlation among multiple diameter measurements on an individual stem. The simple stem taper model was fitted to an extended data set collected across the province of Quebec, Canada. Comparison of the predicted stem taper and the derived stem volume with those obtained using existing models showed a comparable predictive power for the simple model. Including a prediction of the tree random effects based on supplementary diameter measurements of the bole improves the predictive ability of the model around the extra diameter observation. This model offers welcome simplicity as a means of predicting tree taper at coarse resolution for planning tree harvesting.  相似文献   

Short term effects of alternative silvicultural treatments on stand attributes in irregular balsam fir-black spruce stands     

Jérôme Cimon-Morin  Jean-Claude Ruel  Marcel Darveau 《Forest Ecology and Management》2010

The eastern Canadian boreal forest exhibits a specific disturbance regime where forest fires are less frequent than in the western part. This particularity may explain the abundance of irregular stands with distinct ecological features. To ensure sustainable forest management, these characteristics require the implementation of an adapted silviculture regime. In this context, two selection cutting methods were developed and compared with more conventional techniques, initially designed for cutting more regular stands of the boreal forest (cutting leaving small merchantable stems, careful logging preserving advance regeneration). The comparison focused on the capacity of treatments to maintain the primary attributes of irregular boreal forests, including complex vertical structure, abundant tree cover, species composition, and an abundance of dead wood. Mortality and regeneration processes were also compared.  相似文献   

Use of molecularly imprinted solid-phase extraction sorbent for the determination of four 5-nitroimidazoles and three of their metabolites from egg-based samples before tandem LC-ESIMS/MS analysis   总被引：1，自引：0，他引：1  

Mohamed R  Mottier P  Treguier L  Richoz-Payot J  Yilmaz E  Tabet JC  Guy PA 《Journal of agricultural and food chemistry》2008,56(10):3500-3508

A nitroimidazole, molecularly imprinted polymer (MIP) was tested to extract four 5-nitroimidazoles (i.e., dimetridazole (DMZ), ipronidazole (IPZ), metronidazole (MNZ), and ronidazole (RNZ)) and three of their metabolites (i.e., DMZOH, IPZOH, and MNZOH) from egg powder samples. Various MIP templates were produced, and their selectivity was assessed on nitroimidazole standard solutions using liquid chromatography coupled with ultraviolet detection. The optimal cleanup was then used for the extraction of nitroimidazole in egg powder samples, and their quantification was achieved by isotope dilution LC-ESIMS/MS. The sample preparation entails a solubilization of the samples with water and acetonitrile followed by a MISPE cleanup step before LC-ESIMS/MS analysis. Data acquisition was achieved using selected reaction monitoring, and quantification was done with five deuterated analogues (i.e., DMZ- d(3), RNZ- d(3), IPZ- d(3), DMZOH- d(3), and IPZOH- d(3)). DMZOH- d(3) was used to quantify MNZ and MNZOH since they do not have their corresponding internal standards. The method was validated according to the European Union criteria by spiking experiments at concentration levels of 1, 2, and 3 microg/kg. At these three levels and for compounds having their own internal standards, acceptable performance data were obtained, with internal standard corrected recoveries ranging from 91 to 111%, and decision limits (CCalpha) and detection capabilities (CCbeta) were below 0.34 and 0.39 microg/kg, respectively.  相似文献   

Differential aphicidal effects of chitinase inhibitors on the polyphagous homopteran Myzus persicae (Sulzer)     

Saguez J  Dubois F  Vincent C  Laberche JC  Sangwan-Norreel BS  Giordanengo P 《Pest management science》2006,62(12):1150-1154

Four chitinase inhibitors, cyclo-(Proline-Tyrosine), cyclo-(Histidine-Proline), allosamidin and psammaplin A, were selected for in vitro feeding experiments with the peach-potato aphid, Myzus persicae (Sulzer), under controlled photoperiod and temperature conditions. Artificial diets were used to provide chitinase inhibitors at 10, 50 and 100 microg mL(-1) to M. persicae. Except for cyclo-(Proline-Tyrosine), which did not modify aphid demographic parameters, chitinase inhibitors induced differential aphicidal effects on M. persicae. At all doses, cyclo-(Histidine-Proline) induced significant effects affecting daily fecundity, intrinsic rate of natural increase (r(m)) and doubling time of population. When compared with the control diet, allosamidin decreased nymph survival and daily fecundity, increasing the doubling time of population from 1 to 1.5 days. Psammaplin A was the most toxic inhibitor when delivered via artificial diet, as it induced the death of all aphids reared at 50 and 100 microg mL(-1). The results demonstrate the potential use of chitinase inhibitors as aphid management tools.  相似文献