Spatial Concepts in Landscape Analysis and Policy: Some Implications of Globalisation     

Simon Swaffield  J?rgen Primdahl 《Landscape Ecology》2005,20(6):773

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RAPD Characterization of Single Females of the Root-knot Nematodes, Meloidogyne Spp.     

Onivaldo Randig  Frédéric Leroy  Philippe Castagnone-Sereno 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(6):639-643

Random amplified polymorphic DNA fingerprinting was performed on single females of root-knot nematodes, Meloidogyne spp., using a new procedure for DNA isolation. One-fourth of the total DNA amount isolated from a single female proved to be sufficient as a template in a polymerase chain reaction. Electrophoretic patterns of the amplified fragments were reproducible between replicates from a single female or sister females from the same progeny, and identical to those obtained with genomic DNA purified from a large number of nematodes. Moreover, a comparative analysis over three successive generations showed stability of the amplification patterns, thus demonstrating the utility of this procedure for epidemiological and ecological studies on root-knot nematodes.  相似文献   

Chromosome analysis of Proteocephalus osculatus (Cestoda: Proteocephalidea).     

R Petkevici?té 《Folia parasitologica》2001,48(2):159-161

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Leek Proliferation: A New Phytoplasma Disease in the Czech Republic and Italy     

A. Bertaccini  J. Fránová  S. Paltrinieri  M. Martini  M. Navrátil  C. Lugaresi  J. Nebesárová  M. Simkova 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(5):487-493

During the summer 1996, twelve of twenty-eight leek plants located in a garden near eské Budjovice, South Bohemia exhibited symptoms typical of diseases associated with phytoplasmas. In summer 1998 similar symptoms were detected in leek plants in a field used for seed production located in Romagna, North Italy. In both cases the plants were established in the spring of the previous year. Plants showed flower abnormalities: stamen elongation, anther sterility, pistil proliferation, as well as poor, if any, seed production. Phytoplasma-like structures were detected by scanning and transmission electron microscopy in phloem sieve elements in the Czech diseased plants, but not in healthy ones. Nested-PCR amplifications of extracted DNA with phytoplasma-specific oligonucleotide primer pairs confirmed the presence of phytoplasmas in these plants at low concentrations. Restriction fragment length polymorphism analyses of amplified ribosomal sequences allowed the identification of detected phytoplasmas: all the samples from the Czech Republic contained aster yellows related phytoplasmas (16SrI-B) while in the Italian samples aster yellows related phytoplasmas (16SrI-B) together with stolbur related phytoplasmas (16SrXII-A) were identified. This is the first report of detection and identification of a phytoplasma disease of leek in the Czech Republic and Italy.  相似文献   

Regulation by gonadal steroids of estrogen and progesterone receptors along the reproductive tract in female lambs.   总被引：2，自引：0，他引：2  

A Meikle  EG Garófalo  M Rodríguez-Pi?ón  C Tasende  L Sahlin 《Acta veterinaria Scandinavica》2001,42(1):161-169

The regulation of estrogen and progesterone receptor (ER, PR) expression by estradiol (E2) and progesterone (P4) in the oviduct, uterus and cervix of female lambs was studied. The animals received three intramuscular injections of E2, P4 or vehicle with an interval of 24 h and they were slaugthered 24 h after the third injection. Determinations of ER and PR were performed by binding assays and mRNAs of ER alpha and PR by solution hybridization. High levels of ER and PR in both cervix and oviduct were found in the female lamb, differing from other mammalian species. No significant effects by either E2 or P4 treatment on ER and PR levels in the cervix and oviduct could be observed. E2 treatment increased the mRNA levels of ERa and PR more than 3-fold in the cervix, while P4 treatment increased the mRNA levels of ERa and PR in the uterus. The results show differential effects of gonadal steroids on sex steroid receptor expression along the reproductive tract in female lambs, suggesting that steroid target tissues can modulate responses to the same circulating levels of steroid hormones.  相似文献   

An air gun projectile in the ureter of a cat     

H R?cken 《Berliner und Münchener tier?rztliche Wochenschrift》1986,99(11):374-376

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CO2-stunning of swine for slaughter from the anesthesiological viewpoint     

W Erhardt  C Ring  H Kraft  A Schmid  H M Weinmann  R Ebert  B Schl?ger  M Schindele  R Heinze  N Lomholt 《DTW. Deutsche tier?rztliche Wochenschrift》1989,96(3):92-99

For investigations of CO2-stunning of feeder- and slaughter-pigs parameters of behaviour, blood-gas-analyses and electroencephalograms were chosen. The following results were obtained: 1. Blood-gas-analyses proved that the CO2-stunning does not produce unconsciousness due to a lack of oxygen. 2. The criterias of general anaesthesia: unconsciousness, muscle-relaxation and analgesia with total reversibility could be confirmed. 3. The violent convulsive symptoms were evaluated as reactions identical with the stage II of GUEDEL's scheme of anaesthesia. 4. Muscular agitation, which sometimes appeared a few seconds before the stage of excitation, was judged to belong either to the start of the excitation phase or to the end of Guedel's stage of analgesia, during which the sensitivity is decreased. Neither study of behavior nor objective measurements showed, during the first 10 to 20 seconds of exposure to the CO2, any sign of pain or suffering related to the Act for Prevention of Cruelty to Animals, and accordingly such suffering should not be ascribed to the CO2 stunning method.  相似文献   

Abscess forming mandibular osteomyelitis following actinomycosis     

K Nuss  E Sch?ffer  R G K?stlin 《Tier?rztliche Praxis》1989,17(1):15, 109-15, 111

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Chemical determination of the streptothricin antibiotic nourseothricin     

W R?mer  G Hesse  N Miosga  H Fricke 《Archiv fuer experimentelle veterinaermedizin》1986,40(5):693-698

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Comparative in vitro study of the antimicrobial effectiveness of Novugen     

L O Zwillenberg  G B?siger 《Schweizer Archiv für Tierheilkunde》1986,128(2):99-102

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