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991.
The vitamin E distribution in serum, liver, adipose and muscle tissues in the pig during depletion and repletion 总被引:1,自引:0,他引:1
This study was designed to examine the storage capacity for vitamin E in liver, adipose tissue and skeletal muscle of growing pigs during a period of supplementation and of depletion. Therefore, biopsy specimens of these tissues and samples of serum were frequently taken from 7 pigs throughout the experimental period. After a 7-week period on a diet highly supplemented with vitamin E (405 mg/kg), a significant increase (p less than 0.001) in the concentration of this vitamin was observed in all tissues sampled. The highest level (102.9 +/- 26.2 mg/kg) was observed in the liver. After 2 days of depletion the concentration of vitamin E in the liver had fallen by 80%, whereas the concentration in the fat and muscle remained unchanged during 1 week of depletion. The serum vitamin E value rose significantly (p less than 0.001) after 1 week on the supplemented diet and then remained at about 7 mg/l throughout the supplementation period and decreased (p less than 0.001) after 2 days on a nonsupplemented diet. Generally, the serum vitamin E levels among growing pigs are between 2 and 3 mg/l. The results show that the serum and liver values were correlated when the serum level was within this range. Moreover, it is clearly demonstrated that the concentrations of vitamin E in serum and liver reflect the immediate nutritional status of the animal, whereas the vitamin concentrations in adipose and skeletal muscle tissue reflect its long-term nutritional history. 相似文献
992.
J G Cunningham D Haidukewych H A Jensen 《Journal of the American Veterinary Medical Association》1983,182(10):1091-1094
Fifteen dogs with idiopathic epilepsy were included in a 9-month clinical trial to determine the therapeutic serum concentrations of primidone and its active metabolites, phenobarbital and phenylethylmalonamide. Dogs with a seizure frequency greater than 1/mo or with a record of multiple seizures greater than 1/day were chosen for the study. Each dog was given primidone 3 times daily at dosages intended to maximize seizure control and to minimize undesired side effects. Maintenance period blood samples were taken from fasted dogs 7 hours after dosing in the 3rd, 5th, 7th, and 9th months of the trial to determine therapeutic serum concentrations of primidone and its metabolites. Two blood samples also were taken from all dogs 7 hours after dosing, during an enforced drowsy period, to establish upper limits of desirable serum concentrations of the drug. Seizure frequencies during the trial were controlled in 13 dogs, 7 of which had no seizures during the 9-month trial. The mean percentage reduction in seizure frequency from pretrial frequency was 85%. Two dogs appeared refractory to primidone therapy. Serum phenobarbital was the best metabolite of primidone to use to assess therapeutic serum concentrations. The therapeutic antiepileptic serum concentration of phenobarbital was found to be between 25 and 40 micrograms/ml of serum. Serum phenobarbital concentrations greater than 40 micrograms/ml resulted in side effects in most dogs. 相似文献
993.
994.
H E Jensen H V Krogh H Sch?nheyder 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(1):33-40
The agreement between three diagnostic methods (mycology, morphology, and immunofluorescence staining) was compared two by two in elucidating the aetiological diagnosis in 52 cases of bovine mycotic abortion. Agreement between the three methods was expressed by the kappa coefficient (kappa), and ranked as follows: mycology and immunofluorescence staining (kappa = 0.48); morphology and immunofluorescence staining (kappa = 0.40); mycology and morphology (kappa = 0.28). A. fumigatus was confirmed to be the main cause of bovine mycotic abortion with members of the zygomycetes (order Mucorales) being the second most important group. In two aborted placentas a Candida species was found to be the cause of mycotic placentitis. 相似文献
995.
996.
997.
Aspergillus fumigatus is an important agent of mycotic infection in cattle and a potent source of antigens. However, the efficacy of serological diagnosis of aspergillosis in cattle remains controversial. Corbel (1972) and Knudtson et al. (1974) considered a precipitin assay useful as a supplementary test in the diagnosis of mycotic abortion, whereas Wiseman et al. (1984) found the specificity too low to justify its routine use. We have studied 1) the antibody response to the catalase antigen of A. fumigatus in experimentally infected cattle and 2) the prevalence of catalase antibodies and A. fumigatus precipitins in healthy and diseased cattle. The aim was to ascertain how far detection of antibodies to a defined fungal antigen can contribute to the often difficult diagnosis of mycosis. 相似文献
998.
The purpose of these investigations was to develop a modified whole blood technique for measuring quantitatively the responsiveness of pig peripheral blood lymphocytes to phytohaemagglutinin (PHA) in vitro. Washed blood cells from a fixed volume of blood were suspended in culture medium supplemented with foetal calf serum and stimulated with a pure mitogenic PHA preparation. The stimulation was measured by the incorporation of 3H-thymidine.Data are presented to show the effect of different variables on the culture system. The transformation response was measured at different levels of PHA and the reproducibility of the different responses from repeated investigations was used to evaluate the usefulness of the test.The best reproducibilities of the stimulation response were found at the high PHA concentrations.Also the calculated PHA concentration giving maximum stimulation response had a relatively high reproducibility and indicates that this value is a convenient and reliable alternative measure of the functional capacity of the lymphocytes. 相似文献
999.
The purpose of the paper is to describe research carried out in Denmark in areas associated with PSE.In the carcass, meat colour is used as an expression of meat quality. Meat quality deteriorated until 1972 but has improved substantially during the last 6–7 years.The incidence of halothane susceptibility in the Danish Landrace breed is low. Approximately 60% of the halothane-susceptible pigs had PSE carcasses compared to 22% for the non-susceptible pigs. However, pigs heterozygous at the Hal locus are intermediate in meat quality, and elimination of the gene for halothane susceptibility will lead to a substantial improvement in meat quality. A proportion of halothane-susceptible pigs as well as heterozygous carriers can be detected indirectly by using the H blood group system alone or in combination with the PHI system. This is due to linkage disequilibrium between the three systems within the Danish Landrace breed. Use of the H and PHI systems and the halothane test in combination with full sib information on KK index may increase the genetic gain by 5–30%. However, a greater extra gain is easily obtained by a slight increase in selection intensity based on the KK index alone. Thus, it does not seem profitable to use blood and enzyme types or halothane test as a routine in selection against PSE under Danish conditions. In contrast, these tests are useful within herds with a high frequency of PSE. 相似文献
1000.
Direct catalytic hydration of terminal alkenes to primary alcohols would be an inexpensive route to industrially useful alcohols and a convenient synthetic route for the synthesis of terminal alcohols in general. The reaction between trans- PtHCl(PMe(3))(2) (where Me = CH(3)) and sodium hydroxide in a one-to-one mixture of water and 1-hexene yields a species that, at 60 degrees C and in the presence of the phasetransfer catalyst benzyltriethylammonium chloride, catalyzes selective hydration of 1-hexene to n-hexanol at a rate of 6.9 +/- 0.2 turnovers per hour. Hydration of 1-dodecene to n-dodecanol occurs at a rate of 8.3 +/- 0.4 turnovers per hour at 100 degrees C. Deuterium labeling experiments with trans-PtDCl(PMe(3))(2) show that hydration involves reductive elimination of a C-H bond. At low hydroxide concentrations (<8 equivalents), hydration of the water-soluble olefin 3-butene-1-ol to 1,4-butanediol exhibited a first-order dependence on hydroxide concentration for loss of catalytic activity. This suggests that hydroxide attacks the coordinated alkene slowly. At high hydroxide concentrations, the rate of catalysis was hydroxide-independent and first order in alkene. Substitution of coordinated water (k(1) = 9.3 +/- 0.5 x 10(-3) liters per mol per second) appears to be limitng under these conditions. 相似文献