Suvanine Sesterterpenes from a Tropical Sponge Coscinoderma sp. Inhibit Isocitrate Lyase in the Glyoxylate Cycle     

So-Hyoung Lee  Tae Hyung Won  Heegyu Kim  Chan-Hong Ahn  Jongheon Shin  Ki-Bong Oh 《Marine drugs》2014,12(10):5148-5159

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Prevalence and different characteristics of two serotypes of Streptococcus parauberis isolated from the farmed olive flounder, Paralichthys olivaceus (Temminck and Schlegel), in Korea     

Han SY  Kang BK  Kang BJ  Shin SP  Soen BH  Kim JM  Kim JH  Choresca CH  Han JE  Jun JW  Park SC 《Journal of fish diseases》2011,34(10):731-739

The prevalence of two serotypes of Streptococcus parauberis isolated from the olive flounder, Paralichthys olivaceus, was evaluated in a total of 29 isolates between 2003 and 2010 in Korea. Streptococcus parauberis isolates were divided into two serologically distinct types (serotype 1 and serotype 2), except for one strain (S1091), using an agglutination assay with rabbit antiserum, and serotype 1 was identified as the dominant type (24 of 29 isolates) in this study. To identify the characteristics of the two serotypes of S. parauberis, we conducted a biochemical test using the API 20 Strep kit, a transmission electron microscopy (TEM) assay, sequence analysis of 16S‐23S rRNA intergenic spacer region (ISR) and a pathogenicity test. In TEM, both serotypes possessed polysaccharide capsule layers around the cell surface when bacterial cells were treated with a homologous serotype of rabbit antiserum. However, we were unable to discriminate serotype‐specific biochemical characteristics and genetic characteristics of 16S‐23S rRNA ISR between the two serotypes. In the pathogenicity test, the serotype 1 strains induced significantly higher mortality than the serotype 2 strains in olive flounder when experimentally inoculated via the intraperitoneal route.  相似文献   

Lyme disease: Laboratory diagnosis of infected and vaccinated symptomatic dogs     

Richard H. Jacobson  Yung-Fu Chang  Sang J. Shin 《Clinical Techniques in Small Animal Practice》1996,11(3):172-182

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Effect of bovine herpesvirus-1 or parainfluenza-3 virus on immune receptor-mediated functions of bovine alveolar macrophages in the presence or absence of virus-specific serum or pulmonary lavage fluids collected after virus infection     

T T Brown  K Shin 《American journal of veterinary research》1990,51(10):1616-1622

The immune receptor-mediated functions of bovine alveolar macrophages (AM) inoculated in vitro with bovine herpesvirus-1 (BHV-1) or parainfluenza-3 (PI-3) virus were tested in the presence or absence of virus-specific antiserum or pulmonary lavage fluids collected from calves 6 days after inoculation with BHV-1 or PI-3 virus. The Fc and C3b phagocytic indices of noninoculated AM, collected from 6- to 16-week-old calves, ranged from 75 to 87 and 59 to 64, respectively, and the binding indices ranged from 5 to 8 and 22 to 28, respectively. Infection of AM with either BHV-1 or PI-3 virus had no significant effect on receptor-mediated phagocytosis or binding, with the exception of a significant (P less than 0.05) decrease, from 64 to 46, of the C3b phagocytic index of PI-3 virus-infected AM. The addition of lavage fluids, collected after BHV-1 or PI-3 virus infection, to AM infected with the respective virus caused a significant (P less than 0.05) decrease in phagocytic indices with values for the Fc and C3b indices in BHV-1-infected AM decreasing from 81 to 49 and from 47 to 8, respectively, and those for the PI-3 virus-infected AM from 79 to 51 and from 46 to 15, respectively. The binding indices of virus-infected AM increased with the addition of viral lavage fluids, but the only significant (P less than 0.05) increase was for C3b binding in PI-3 virus-infected cells, which increased from 33 to 56.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Pharmacokinetic variables of moxifloxacin in healthy male camels following intravenous and intramuscular administration     

Abd El-Aty AM  Goudah A  Shah SS  Shin HC  Shimoda M  Shim JH 《Journal of veterinary pharmacology and therapeutics》2007,30(6):586-591

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Chromosomes responsible for sensitivity of embryo to abscisic acid and dormancy in wheat   总被引：4，自引：0，他引：4  

Kazuhiko Noda  Takakazu Matsuura  Masahiko Maekawa  Shin Taketa 《Euphytica》2002,123(2):203-209

The sensitivity of the embryo to abscisic acid (ABA) has been reported toplay an important role in seed dormancy. Using ditelocentric lines of wheatcv. Chinese Spring (CS, nondormant and ABA insensitive), F₂ seedsbetween monosomic lines of CS and a wheat line Kitakei-1354 (dormant,ABA sensitive) and deletion lines of CS chromosome 4A, germinability ofseeds and embryo-half seeds incubated in water and ABA were examined. The results indicated that the long arm of chromosome 4A carried majorgene(s) for the embryo sensitivity to ABA and dormancy. Chromosome2D might be also involved in the sensitivity to ABA.  相似文献   

A molecular technique for selection of self-compatible varieties of Japanese pear (Pyrus pyrifolia Nakai)     

Hoy-Taek Kim  Yutaka Hirata  Yong-Uk Shin  Hae-Sung Hwang  Jeong-Hwan Hwang  Il-Sheob Shin  Dae-Il Kim  Sang-Jo Kang  Hyun-Jung Kim  Dong-Young Shin  Ill-Sup Nou 《Euphytica》2004,138(1):73-80

The pear cultivar ‘Osa-Nijisseiki’ (S₂S^sm ₄; sm = stylar-part mutant) has been used as a parent to breed self-compatible cultivars that produce excellent fruits. However, determination of the self-compatibility of ‘Osa-Nijisseiki’ offspring requires a lot of time, 6 years or more, by conventional cross breeding. We have designed a rapid reliable method for the identification of self-compatible varieties of ‘Osa-Nijisseiki’ offspring based on the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with S-allele specific restriction endonucleases. By using this method, 8 self-compatible varieties were selected among 16 selections resulting from a cross between the self-compatible cultivar ‘Osa-Nijisseiki’ (S₂S^sm ₄) and the self-incompatible cultivars ‘Niitaka’ (S₃S₉), ‘Whasan’ (S₃S₅), ‘Chuwhangbae’ (S₄S₆). The S-genotypes of 16 ‘Osa-Nijisseiki’ offsprings were also determined. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Color characteristics of monascus pigments derived by fermentation with various amino acids     

Jung H  Kim C  Kim K  Shin CS 《Journal of agricultural and food chemistry》2003,51(5):1302-1306

Various pigment colors were produced by Monascus fermentations with separate addition of 20 amino acids. The color characteristics and structures of the pigment derivatives were investigated. When each amino acid was added to the fermentation broth as a precursor, pigment extracts with different hue and chroma values were obtained depending on the content ratios of yellow, orange, and red colors in the fermentation broth. The yellow and orange pigments were identical regardless of amino acid addition. The red compounds varied on the basis of the type of amino acid added. LC-MS and (1)H and (13)C NMR structural analyses confirmed that the derivative pigments contained the moieties of the added amino acids. L, a, and b values of the CIELAB color system for the derivative pigments were measured. Values of hue and chroma were then calculated. The colors of the derivative pigments were in the range of orangish red to violet red. The hydrophilicities/hydrophobicities of the derivative pigments could be predicted from their log P values, which were estimated using computer programs.  相似文献   

Management of pine wilt disease in Korea through preventative silvicultural control   总被引：3，自引：0，他引：3  

Tae-Sung KwonJoon Hwan Shin  Jong-Hwan LimYoung-Kul Kim  Eun Ju Lee 《Forest Ecology and Management》2011,261(3):562-569

Pine wilt disease (PWD) represents a major threat to forest ecosystems worldwide. Although PWD is now better understood, effective control measures for this disease have still not been devised. Here, we report several years of field studies on preventative silvicultural control of PWD. Silvicultural control through preventative clear-cutting and the manual removal of logs was implemented between 2005 and 2009 in 16 Korean districts that had newly PWD-infected stands. Preventative clear-cutting of neighboring asymptomatic pine trees (within a 10-50-m radius of wilt trees) and the removal of felled logs or branches suppressed spread of PWD. Occurrences of PWD wilt pines in districts (city or county) subjected to this silvicultural control method were significantly reduced compared with those in districts using conventional controls (physical or chemical treatment of wilt pine trees). Through silvicultural control, PWD was successfully suppressed in 11 of 16 districts investigated. In contrast, successful control was achieved in only 1 among 18 districts subjected to conventional control. Our results will be of considerable interest to those engaged in the very difficult battle against the global spread of PWD.  相似文献   

Breakdown and macroinvertebrate colonization of needle and leaf litter in conifer plantation streams in Shikoku,southwestern Japan     

Mayu Hisabae  Shin Sone  Mikio Inoue 《Journal of Forest Research》2011,16(2):108-115

Breakdown and macroinvertebrate colonization of conifer needles (Cryptomeria japonica) and deciduous broadleaves (Euptelea polyandra) were investigated using litter bags in two study sites in streams flowing through a conifer plantation of C. japonica in Shikoku, southwestern Japan (one site with conifer canopy and another with mixed conifer and broadleaved canopy). Breakdown rates and macroinvertebrate densities were compared between litter species (conifer needle vs broadleaf) and between the two sites (conifer vs mixed canopy) to determine (1) whether breakdown rate of broadleaves is higher than conifer needles, (2) whether macroinvertebrates prefer broadleaves to conifer needles, and (3) whether the difference in riparian canopy is reflected in macroinvertebrate abundance. The results indicated that breakdown rates of broadleaves were higher than those of conifer needles, suggesting poorer quality of the latter as food for macroinvertebrates. Differences in macroinvertebrate density between needles and broadleaves were generally consistent with those in breakdown rates: broadleaves tended to have higher densities than needles, suggesting that conifer needles were not preferred by macroinvertebrates. However, total macroinvertebrate density in the conifer site was not significantly different from that in the mixed site, although the dominant shredder taxon differed (conifer site: gammarids; mixed site: lepidostomatids). Although conifer needles are low-quality food for macroinvertebrates, this may offer some advantages. Conifer needles remain on the streambed for longer periods owing to their lower breakdown rates, being a constantly available resource. In addition, accumulations of conifer litter may effectively trap and retain particulate organic matter.  相似文献