The coat proteins and putative movement proteins of isolates of Prunus necrotic ringspot virus from different host species and geographic origins are extensively conserved     

S.W. Scott  M.T. Zimmerman  Xin Ge  D.J. MacKenzie 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(2):155-161

A complete sequence for the RNA 3 of Prunus necrotic ringspot virus (PNRSV) is described (Genbank Accession U57046). Primers from this sequence were used to amplify both the movement protein and coat protein genes of 3 other isolates of PNRSV originating from different host species and geographic locations. Comparisons of these sequences with those of other published sequences for PNRSV and the closely related apple mosaic virus (ApMV) showed that both the movement proteins and coat proteins of isolates of PNRSV are extensively conserved irrespective of either the original host or the geographic origin. The movement protein and coat protein of ApMV and PNRSV are sufficiently conserved to suggest that these two viruses may have evolved from a common ancestor. The amino acid sequence of the two coat proteins shows areas of similarity and difference that would explain the serological continuum reported to occur among isolates of these two viruses. Nevertheless, the movement protein and coat protein of the two viruses are sufficiently different so that ApMV and PNRSV should be considered to be distinct viruses.  相似文献   

鸡贫血病雏鸡ND免疫后局部免疫组织抗体生成细胞变化   总被引：1，自引：1，他引：0  

郑世民  杨蕊 《黑龙江畜牧兽医》1998,(6):1-3

对感染鸡贫血病毒（ＣＡＶ）雏鸡新城疫（ＮＤ）疫苗免疫及其强毒攻击后局部免疫组织——盲肠扁桃体和哈德尔腺的ＩｇＡ、ＩｇＧ和ＩｇＭ抗体生成细胞数量的动态变化进行了检测。结果发现,ＣＡＶ感染雏鸡ＮＤ免疫后７、１４、２８ｄ及新城疫强毒（ｖＮＤＶ）攻击后,上述免疫组织的三种抗体生成细胞数量均不同程度地低于未感染ＣＡＶ的ＮＤ免疫对照雏鸡。其中盲肠扁桃体弥散区的ＩｇＡ减少最为明显。表明ＣＡＶ感染雏鸡消化道和呼吸道局部免疫组织对新城疫疫苗的体液免疫应答功能降低。新城疫强毒攻击后ＣＡＶ感染ＮＤ免疫雏鸡的免疫保护率也明显低于未感染ＣＡＶ的免疫对照雏鸡。  相似文献   

添加苯二氮化合物F89对皖西白鹅生长和代谢性能的影响     

葛盛芳  葛继志  韩正康  陈杰 《中国家禽》1998,(10)

利用生理调节剂Ｆ８９提高皖西白鹅的摄食量,加速动物生长。日粮中添加２ｍｇ／ｋｇＦ８９,连续饲喂８～３５日龄皖西白鹅,日摄食量较对照组增加６．０２％,增重提高９．４０％,耗料比下降２．８９％。对育肥期鹅的增重也有明显影响。血液代谢物分析表明：饲喂Ｆ８９后,皖西白鹅血清中葡萄糖和尿酸浓度下降,β－脂蛋白浓度上升。提示Ｆ８９不仅能促进鹅的摄食量,而且可以影响鹅的代谢机能,促进生长  相似文献   

Influence of murine cytomegalovirus infection on the differentiation and the differentiation genes expression in neural stem cells     

LUO Dan  SHU Sai-nan  ZHOU Yu-feng  LIU Xing-lou  TIAN Jia  WANG Hui  ZHOU Hua  LI Ge  FANG Feng 《园艺学报》2009,25(11):2173-2181

AIM: The influence of MCMV infection on differentiation and differentiation gene expression in neural stem cells(NSCs) in vitro were investigated for studying the mechanisms of brain abnormalities caused by congenital cytomegalovirus infection.METHODS: NSCs were separated from fetal BALB/C mouse, and cultured and identified in vitro. The differentiation potency of NSCs was observed by immunofluorescence. The NSCs infected by MCMV at dosage of MOI(multiplicity of infection) equaled to 5, 1 and 0.1,respectively, were cultured in differentiation medium. The morphological changes of infected cells were observed under inverted microscope. The ratios of NSCs and its differentiated cells were detected by flow cytometry. The expressions of nestin, GFAP and NSE, markers of NSCs and its differentiated cells, were studied by immunofluorescence(MOI=1). The expression of early antigen(EA) of MCMV was detected to observe the infection process. Real-time RT-PCR method was employed to measure the expression levels of the key genes Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs at early stage of differentiation culture.RESULTS: NSCs isolated from embryonic mouse brains proliferated to form neurospheres, strongly expressed nestin and differentiated into NF-200 positive neurons or GFAP positive astrocytes. The infected NSCs did not adhere to the wall and appeared differentiation growths, but showed swollen gradually after differentiation culture. The nestin expression in the infected cells downregulated slowly and was higher than that in control groups(P<0.05). The GFAP and NSE expressions of the infected cells were lower than those in control groups(P<0.05). The early antigen(EA) of MCMV was always detected in the cells in infected groups. The ratios of nestin positive cells in infected groups were higher than those in control groups, but the ratios of GFAP and NSE positive cells of former were lower than that of the latter from 3rd to 9th d after differentiation culture(P<0.05). The levels of Neurog2 mRNA and Myc mRNA in infected groups were markedly lower than those in normal control groups on 1st d and from 1st to 4th d after differentiation culture, respectively(P<0.05). The levels of Ccnd1 mRNA of infected groups were obviously lower than those in normal control groups from 12th h to 1st d(P<0.05). These changes in infected groups became more obvious as MCMV MOI increased.CONCLUSION: MCMV significantly inhibits differentiation of NSCs to neurons and astrocytes, and leads to the decrease in differentiated cells. MCMV inhibits or interferes with the gene expression of Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs. The effect that MCMV inhibits the expressions of differentiation and the differentiation genes in NSCs shows dose-dependent with MCMV MOI. The inhibitory effect of MCMV on the differentiation of NSCs might be induced by interfering with the expression of differentiation gene in NSCs, which is possibly the one of primary causes of brain development disorders induced by congenital CMV infection.  相似文献   

大肠杆菌内毒素多克隆抗体的制备与纯化     

李生  吕娟  胡格  索占伟  段慧琴  穆祥 《勤云标准版测试》2007,(11)

为制备大肠杆菌内毒素多克隆抗体,应用大肠杆菌内毒素作为抗原免疫5周龄家兔,辛酸-硫酸铵法和葡聚糖凝胶层析法分离提纯抗血清,十二烷基磺酸钠-聚丙烯酰胺凝胶(SDS-PAGE)电泳方法鉴定多抗纯度,紫外分光光度计测定抗体蛋白含量,ELISA法检测抗血清效价与交叉反应;大肠杆菌内毒素抗体含量和效价分别为6.95mg/ml和1:12800;成功制备出抗大肠杆菌内毒素多克隆抗体,为大肠杆菌内毒素病诊断试剂盒的研制奠定基础。  相似文献   

克氏原螯虾温室大棚规模化繁育技术及工艺     

赵朝阳  周鑫  徐增洪  徐跑  戈贤平 《勤云标准版测试》2009,29(5)

阐述了克氏原螯虾温室大棚规模化繁育技术,包括繁育设施的建造、工厂化人工诱导繁殖,规模化网箱育苗技术等,为克氏原螯虾育苗生产提供借鉴.  相似文献   

密度钳法细羊毛密度测定根数的探讨     

杨光  李英 《新疆农业大学学报》1998,21(1):80-81

本研究通过对不同抽测根数细羊毛密度的测定，并以国家标准为对照，认为在实际育种工作中，个体羊毛密度从根部抽测２００～３００根即可。  相似文献   

植物中γ-亚麻酸合成关键酶——△^6-脂肪酸脱氢酶     

盖燕  刘蕾  何聪芬 《广西农业生物科学》2009,(4):809-814

γ-亚麻酸作为人体必需的不饱和脂肪酸，对人体的激素调节及脂肪酸代谢发挥着重要的生理作用。△^6-脂肪酸脱氢酶是多不饱和脂肪酸合成途径中的限速酶。本文介绍了不饱和脂肪酸γ-亚麻酸代谢途径中的关键酶△^6-脂肪酸脱氢酶的结构功能与目前△^6-脂肪酸脱氢酶的基因工程研究进展，并对其应用进行了展望，以期为利用基因工程手段生产γ-亚麻酸提供参考。  相似文献   

浅谈造价工程师在监理工作中的地位和作用     

葛蔚 《林业科技情报》2007,39(3):85-86

在监理工作中配备既懂工程技术又懂经济、管理和法律知识,具有实践经验和良好职业道德素质的复合型人才一造价工程师,是非常必要的.  相似文献   

性信息素诱捕防治棉红铃虫   总被引：1，自引：0，他引：1       下载免费PDF全文

李文谷  郦一平  葛世华  林维先  李仁龙 《植物保护学报》1985,12(3):145-149

1980～1981年在浙江海盐和上虞进行了性信息素诱捕防治棉铃虫的大田试验。面积分别为3500、3000亩。试验证明,每亩设置3～4只诱捕盆的大面积诱捕防治能明显降低田间红铃虫雌蛾的交配率(下降68.2～96.7%),棉株受害程度也减轻,与常规农药防治的对照相比,花害率下降42.4～78.9%,铃害率下降30.3～54.4%,同时还减少了用药次数,间接起到保护天敌、减少环境污染的作用。因此认为,性信息素诱捕法有可能作为棉红铃虫综合防治中的一项有效措施。  相似文献