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101.
A simple and very sensitive spectrophotometric method determining microgram quantities of gallium (Ga) has been developed. The method is based on the complex of Ga(III) with Chrome Azurol S (CAS) in the presence of benzyldodecyldimethylammonium bromide (ST) and polyoxyethylenesorbitan monopalmitate (Tween 40). The complex forms in weakly acid solution (pH 3.8) and shows the absorption maximum at 627 nm. The molar absorptivity (ε) of this system is 1.87 × 105 L mol?1 cm?1, and it is clearly higher than ε of other spectrophotometric methods of gallium determination. The detection and quantification limits (LOD, LOQ) are 7.6 × 10–3 and 0.023 µg mL?1, respectively. Beers law is obeyed in the range from 0.02 to 0.60 µg mL?1 of Ga. The precision and accuracy are good. This first spectrophotometric method of Ga(III) determination in the four-component system has been successfully applied for the analysis of mushrooms and grasses with low content of gallium.  相似文献   
102.
The effect of Chenopodium quinoa seeds on lipid profile, glucose level, protein metabolism and selected essential elements (Na, K, Ca, Mg) level was determined in high—fructose fed male Wistar rats. Fructose decreased significantly LDL [42%, p < 0.01] and activity of alkaline phosphatase [20%, p < 0.05], and increased triglycerides level [86%, p < 0.01]. The analysis of blood of rats fed quinoa indicated, that these seeds effectively reduced serum total cholesterol [26%, p < 0.05], LDL [57%, p < 0.008] and triglycerides [11%, p < 0.05] when compared to the control group. Quinoa seeds also significantly reduced the level of glucose [10%, p < 0.01] and plasma total protein level [16%, p < 0.001]. Fructose significantly decreased HDL [15%, p < 0.05] level in control group but when the quinoa seeds were added into the diet the decrease of HDL level was inhibited. Quinoa seeds did not prevent any adverse effect of increasing triglyceride level caused by fructose. It was shown in this study that quinoa seeds can reduce most of the adverse effects exerted by fructose on lipid profile and glucose level.  相似文献   
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Winter oilseed rape (Brassica napus) is an important crop in the Czech Republic and Poland. Clubroot disease caused by the pathogen Plasmodiophora brassicae is a serious and still-growing problem for oilseed rape growers in both countries. The aim of this study was to evaluate the pathotype composition of P. brassicae populations from the Czech Republic and Poland, according to the three evaluation systems, and to determine soil inoculum loads for representative fields via traditional end-point PCR as well as quantitative PCR analysis. There were considerable differences between the populations of P. brassicae from both countries, and the number of pathotypes varied depending on the evaluation system and the threshold used to distinguish susceptible vs. resistant plant reactions. This is the first study comparing the effect of different thresholds. Using an index of disease (ID) of 25 % to distinguish susceptible vs. resistant reactions, there was a total of seven pathotypes identified based on the differentials of Williams, five with the system of Somé et al., and 18 with the European Clubroot Differential (ECD) set. However, based on a threshold of 50 %, there were nine pathotypes according to the evaluation system by Williams, four based on the differentials of Somé et al., and 15 with the ECD set. Changing of the thresholds led to the reclassification of some pathotypes. Several pathotypes were common in both countries. High amounts of pathogen DNA were found in many of the field soils analysed by quantitative PCR. There was a weak correlation between soil pH and infestation of P. brassicae for the Polish soils.  相似文献   
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Changes in the content of carbohydrates and in the activity of hydrolytic enzymes were studied in hyacinth bulbs exposed to low temperatures. It was found that the amount of insoluble carbohydrate initially present in the scales and buds of hyacinth bulbs decreased greatly after a prolonged period of low temperature treatment. This decrease was greater in rooted bulbs, held at a low temperature for 63 days in natural conditions, than in unrooted bulbs stored in a refrigerator for the same period. At the same time the levels of soluble mono- and oligosaccharides, and of soluble starch, increased in the scales and buds of chilled bulbs. These changes in sugar level were found to be correlated with changes in the activity of certain hydrolytic enzymes. The activity of these enzymes was higher in rooted bulbs than in those which had not rooted before treatment. The activity of hydrolytic enzymes was found to be considerably higher in the flower buds than in the scales.  相似文献   
108.
Summary

The goals of this study were to make an inventory of recent and ongoing fruit and fruit product innovations, to assess what novelty or improvement they offer, and whether consumers could identify and/or recognise them. Researchers from 11 European countries submitted 386 examples of fruit and fruit product innovations. The list of innovations obtained has been coded, categorised, sorted, and reduced in subsequent stages. First, the examples received were categorised according to the Oslo Manual definitions. Second, product and marketing innovations were selected, as they are the only ones that were likely to be recognised by consumers. Next, analysis revealed that the novelties these innovations offered related to Convenience, Health, Differentiation, Target Group, Information, Sensory Characteristics, In Home and/or Out of Home Quality. Some innovations offered only one novel aspect, whereas others offered multiple aspects. Interrelationships between novel aspects are discussed for those innovations that offered a combination of aspects.  相似文献   
109.
The study focused on assessing the influence of rhamnolipids on the phytotoxicity of diesel oil-contaminated soil samples. Tests evaluating the seed germination and growth inhibition of four terrestrial plant species (alfalfa, sorghum, mustard and cuckooflower) were carried out at different rhamnolipid concentrations (ranging from 0 to 1.200?mg/kg of wet soil). The experiments were performed in soil samples with a different diesel oil content (ranging from 0 to 25?ml/kg of wet soil). It was observed that the sole presence of rhamnolipids may be phytotoxic at various levels, which is especially notable for sorghum (the germination index decreased to 41?%). The addition of rhamnolipids to diesel oil-contaminated soil samples contributed to a significant increase of their phytotoxicity. The most toxic effect was observed after a rhamnolipid-supplemented diesel oil biodegradation, carried out with the use of a hydrocarbon-degrading bacteria consortium. The supplemention of rhamnolipids (600?mg/kg of wet soil) resulted in a decrease of seed germination of all studied plant species and an inhibition of microbial activity, which was measured by the 2,3,5-triphenyltetrazolium chloride tests. These findings indicate that the presence of rhamnolipids may considerably increase the phytotoxicity of diesel oil. Therefore, their use at high concentrations, during in situ bioremediation processes, should be avoided in a terrestrial environment.  相似文献   
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