Early origin and recent expansion of Plasmodium falciparum     

Joy DA  Feng X  Mu J  Furuya T  Chotivanich K  Krettli AU  Ho M  Wang A  White NJ  Suh E  Beerli P  Su XZ 《Science (New York, N.Y.)》2003,300(5617):318-321

The emergence of virulent Plasmodium falciparum in Africa within the past 6000 years as a result of a cascade of changes in human behavior and mosquito transmission has recently been hypothesized. Here, we provide genetic evidence for a sudden increase in the African malaria parasite population about 10,000 years ago, followed by migration to other regions on the basis of variation in 100 worldwide mitochondrial DNA sequences. However, both the world and some regional populations appear to be older (50,000 to 100,000 years old), suggesting an earlier wave of migration out of Africa, perhaps during the Pleistocene migration of human beings.  相似文献   

High-throughput mapping of a dynamic signaling network in mammalian cells     

Barrios-Rodiles M  Brown KR  Ozdamar B  Bose R  Liu Z  Donovan RS  Shinjo F  Liu Y  Dembowy J  Taylor IW  Luga V  Przulj N  Robinson M  Suzuki H  Hayashizaki Y  Jurisica I  Wrana JL 《Science (New York, N.Y.)》2005,307(5715):1621-1625

Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor-beta (TGFbeta) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFbeta pathway to the p21-activated kinase (PAK) network, to the polarity complex, and to Occludin, a structural component of tight junctions. We show that Occludin regulates TGFbeta type I receptor localization for efficient TGFbeta-dependent dissolution of tight junctions during epithelial-to-mesenchymal transitions.  相似文献   

Graduate students' teaching experiences improve their methodological research skills     

Feldon DF  Peugh J  Timmerman BE  Maher MA  Hurst M  Strickland D  Gilmore JA  Stiegelmeyer C 《Science (New York, N.Y.)》2011,333(6045):1037-1039

Science, technology, engineering, and mathematics (STEM) graduate students are often encouraged to maximize their engagement with supervised research and minimize teaching obligations. However, the process of teaching students engaged in inquiry provides practice in the application of important research skills. Using a performance rubric, we compared the quality of methodological skills demonstrated in written research proposals for two groups of early career graduate students (those with both teaching and research responsibilities and those with only research responsibilities) at the beginning and end of an academic year. After statistically controlling for preexisting differences between groups, students who both taught and conducted research demonstrate significantly greater improvement in their abilities to generate testable hypotheses and design valid experiments. These results indicate that teaching experience can contribute substantially to the improvement of essential research skills.  相似文献   

Species-specific sensitivity of earthworm coelomocytes to dermal metal (Cd,Cu, Ni,Pb, Zn) exposures: Methodological approach     

Barbara Plytycz  Malgorzata Klimek  Joanna Homa  Agnieszka Irena Mazur  Jerzy Kruk  A. John Morgan 《Pedobiologia》2011

Earthworm immune-competent cells (coelomocytes) can be adversely affected by soil metal contamination. The aim of the present paper was to perform comparative studies on the coelomocytes of four lumbricid species dermally exposed for 2 days to filter paper soaked with Ni, Cu, Zn, Cd, or Pb chlorides (1 mM). Coelomocytes were expelled by electro-stimulation, counted, and assayed ex vivo by flow cytometry and spectrofluorometry. The coelomocytes of Allolobophora chlorotica and Dendrobaena veneta contain both amoebocytes and riboflavin-storing eleocytes; consequently, they were subjected to assays based on flow cytometric quantification of autofluorescent eleocytes and spectrofluorometric measurement of riboflavin content in coelomocyte lysates. In Al. chlorotica, the number of coelomocytes, the percentage of eleocytes, and the amount of riboflavin were significantly lower in Cu-exposed worms although these cytometric parameters were less affected by Ni, Zn, Cd exposure, and entirely unaffected by Pb exposure. In D. veneta, such cellular effects were also only observed in Cu-exposed worms. The coelomic fluids of Aporrectodea caliginosa and Lumbricus rubellus are constitutionally impoverished of eleocytes; therefore, these species could not be subjected to assays measuring the riboflavin content of these granular cells. Rather, the amoebocytes of Ap. caliginosa and L. rubellus were subjected to flow cytometric measurements of in vitro neutral red uptake (NR). However, the NR uptake assay was technically demanding, requiring a strictly normalized incubation period over all samples to yield useful comparative data. In contrast, the riboflavin content in the coelomocyte lysates of eleocyte-rich species appears to be a robust and convenient immune-function biomarker of environmental stress.  相似文献   

A study to evaluate buprenorphine at 40 μg kg(-1) compared to 20 μg kg(-1) as a post-operative analgesic in the dog     

Slingsby LS  Taylor PM  Murrell JC 《Veterinary anaesthesia and analgesia》2011,38(6):584-593

ObjectiveComparison of the analgesic effect of buprenorphine at 20 or 40 μg kg^?1.Study designAn investigator ‘blinded’, randomised study.AnimalsTwenty six dogs presented for ovariohysterectomy.MethodsDogs were premedicated intramuscularly with acepromazine 0.03 mg kg^?1 and buprenorphine at either 20 (B20, n = 12) or 40 μg kg^?1 (B40, n= 14) followed by anaesthetic induction with propofol and maintenance with isoflurane. During anaesthesia non invasive blood pressure, heart rate, respiratory rate, blood oxygen saturation, inspired and expired volatile agent, end-tidal carbon dioxide and ECG were recorded. Pain and sedation were assessed using interactive VAS scores; mechanical nociceptive thresholds were measured at the wound and hindlimb - all were assessed before and up to 22 hours after administration. Carprofen was used for rescue analgesia.ResultsThere were no significant differences between the two groups for any of the parameters examined. Rescue analgesia was required around 5 hours after administration of buprenorphine in a significant number of animals. Sedation was good preoperatively and scores decreased over time postoperatively. Hock thresholds did not change over time; wound thresholds decreased significantly compared to the baseline value from 3 hours onwards.ConclusionsAdministration of buprenorphine at either 20 or 40 μg kg^?1 IM with acepromazine provided good pre-operative sedation. Cardiovascular and respiratory values remained within clinically acceptable limits during anaesthesia. There was no evidence that increasing dose increased adverse events that may be associated with opioid administration (e.g. bradycardia and respiratory depression).Clinical relevanceIncreasing the dose of buprenorphine from 20 to 40 μg kg^?1 did not provide any benefits with respect to analgesia after ovariohysterectomy as assessed using the VAS scoring system.  相似文献   

Does the definition of the Mediterranean diet need to be updated?     

Serra-Majem L  Trichopoulou A  Ngo de la Cruz J  Cervera P  García Alvarez A  La Vecchia C  Lemtouni A  Trichopoulos D;International Task Force on the Mediterranean Diet 《Public health nutrition》2004,7(7):927-929

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Nutrient cycling in a Pinus patula plantation in the Mpumalanga Province, South Africa     

Joanna F. Dames  Mary C. Scholes  C. J. Straker 《Applied soil ecology》2002,20(3):211-226

The accumulation of litter on the forest floor was identified as a potential problem in managed plantations of Pinus patula (Schlechtd. et Cham.) in the Mpumalanga Province of South Africa in the late 1980s. Litter accumulation in pine plantations is regarded as a threat to site productivity as organic acids are released, moisture penetration is altered and nutrients are immobilised within the litter. This study examines the cycling of nutrients in a 42-year-old P. patula stand in which litter has accumulated. Samples of the vegetation, litter and soil components were collected and chemically analysed for total nitrogen (N), phosphorus (P) and the major cations potassium (K⁺), calcium (Ca²⁺) and magnesium (Mg²⁺). Complete nutrient budgets for N and P, and the cation pool sizes were determined. It was evident from these studies that large reserves of N (1442 kg ha⁻¹) and P (103 kg ha⁻¹) are stored in the litter layers, with levels of cations being low. The presence of large nutrient reserves within the litter and the predominance of fine feeder roots distributed within this layer indicated that a tightly closed plant–litter–plant nutrient cycle was in operation for the cycling of N and P. This may not be true for the major cations. Management of the litter should ensure retention of as many nutrients as possible in the system. This could be achieved through controlled burning to reduce nutrient loss through volatilisation; increasing forest floor temperatures by altering the planting density and application of dolomitic lime to replace cations and to alleviate the acidic conditions making the litter more favourable for decomposing organisms.  相似文献   

Food, youth and the Mediterranean diet in Spain. Development of KIDMED, Mediterranean Diet Quality Index in children and adolescents     

Serra-Majem L  Ribas L  Ngo J  Ortega RM  García A  Pérez-Rodrigo C  Aranceta J 《Public health nutrition》2004,7(7):931-935

OBJECTIVE: To evaluate dietary habits in Spanish children and adolescents based on a Mediterranean Diet Quality Index tool, which considers certain principles sustaining and challenging traditional healthy Mediterranean dietary patterns. DESIGN: Observational population-based cross-sectional study. A 16-item Mediterranean Diet Quality Index was included in data gathered for the EnKid study (in which two 24-hour recalls, a quantitative 169-item food-frequency questionnaire and a general questionnaire about socio-economic, demographic and lifestyle items were administered). SETTING: Spain. SUBJECTS: In total, 3850 children and youths aged 2-24 years residing in Spain. RESULTS: Of the sample, 4.2% showed very low KIDMED index results, 49.4% had intermediate values and 46.4% had high index results. Important geographical differences were seen, with subjects from the Northeast showing the most favourable outcomes (52% with elevated scores vs. 37.5% of those from the North). Lower percentages of high diet quality were observed in low socio-economic groups, compared with middle and upper income cohorts (42.8%, 47.6% and 54.9%, respectively). Large cities had more positive results and only slight variations were seen for gender and age. CONCLUSIONS: The KIDMED index, the first to evaluate the adequacy of Mediterranean dietary patterns in children and youth, confirms that this collective is undergoing important changes, which makes them a priority target for nutrition interventions. Results challenge certain commonly perceived notions tied to income level, population size and diet quality.  相似文献   

Cut-flower life of dry transported carnations as influenced by different silver form pre-treatments     

B. Le Masson  Joanna Nowak 《Scientia Horticulturae》1981,15(4):383-390

Twenty-four hours of pre-treatment with AgNO₃ increased the vase-life of flowers from 3 to 4 days for carnations kept in water or in preservative solutions after simulated transport conditions. A mixture of AgNO₃ with sodium thiosulphate or with EDTA was considerably better than AgNO₃, while such complexing agents as CDTA, EDDHA and EDPA were less effective. Determination of radioactive silver (Ag) in dissected carnation parts treated with different Ag complexes showed a positive correlation between vase-life and the amount of tracer Ag in the plants.  相似文献   

Investigation of changes in the middle latency auditory evoked potential during anesthesia with sevoflurane in dogs     

Murrell JC  de Groot HN  Psatha E  Hellebrekers LJ 《American journal of veterinary research》2005,66(7):1156-1161

OBJECTIVE: To investigate the middle latency auditory evoked potential (MLAEP) in awake dogs and dogs anesthetized with 2 concentrations of sevoflurane. ANIMALS: 10 adult Beagles. PROCEDURE: The MLAEP was recorded while dogs were awake and anesthetized with sevoflurane (end-tidal concentration, 2.7% or 3.5%).Three needle electrodes were inserted SC, and click stimuli were delivered biaurally. Signal acquisition, averaging, and analysis were performed by use of computer software developed in-house. Signals were recorded for 128 milliseconds, and the responses to 1,024 stimuli were averaged. Waveforms from 10 recordings in each dog were averaged, and latencies of peaks were measured. Data acquired for awake dogs and dogs anesthetized with high and low sevoflurane concentrations were compared statistically. RESULTS: Sevoflurane anesthesia attenuated the MLAEP so that only peaks P0, Na, and Pa could be identified. The MLAEP changes were maximal at the lower concentration of sevoflurane evaluated. The latencies of these peaks were significantly shorter in awake dogs, compared with values in anesthetized dogs. No difference in the peak latency was detected between the sevoflurane concentrations. CONCLUSIONS AND CLINICAL RELEVANCE: In terms of CNS responsiveness, the effects of anesthesia with sevoflurane are similar to those of anesthesia with isoflurane. Data suggest that sevoflurane is not the inhalant agent of choice in a research setting where electroencephalographic measurements are to be recorded during anesthesia. The depression of the MLAEP waveform by sevoflurane also suggests that the MLAEP is not a suitable tool with which to monitor anesthetic depth during sevoflurane anesthesia in dogs.  相似文献