航天诱变对糯玉米主要数量性状及配合力的影响   总被引：3，自引：0，他引：3  

邱正高  杨华  王贵学  柯剑鸿  祁志云  袁亮 《西南农业学报》2009,22(5)

糯玉米纯合自交系S147、S181种子经过航天诱变处理得到的诱变系,按照NCⅡ遗传交配设计,分析航天诱变处理对糯玉米自交系主要农艺性状的诱变效应,结果表明:航天诱变可使纯合玉米自交系的农艺性状产生变异和配合力产生显著变化;S147、S181对航天诱变处理的反应差异明显.  相似文献   

The plasmid pcDNA3 differentially induces production of interferon-alpha and interleukin-6 in cultures of porcine leukocytes   总被引：2，自引：0，他引：2  

Magnusson M  Johansson E  Berg M  Eloranta ML  Fuxler L  Fossum C 《Veterinary immunology and immunopathology》2001,78(1):45-56

An adjuvant effect of invertebrate DNA has been attributed to its relative high frequency of unmethylated CpG dinucleotides. Here we describe the interferon-alpha (IFN-alpha) and interleukin-6 (IL-6) inducing properties of a commonly used eukaryotic expression vector, pcDNA3, in porcine leukocytes. The magnitude of the cytokine response was compared to that induced by the synthetic ds RNA analogue poly(I):poly(C), inactivated preparations of Aujeszky's disease virus (ADV) and the Gram-negative bacteria Actinobacillus pleuropneumoniae. The plasmid, as well as poly(I):poly(C), required lipofectin to induce IFN-alpha production whereas both preparations induced IL-6 irrespective of preincubation with lipofectin. However, the nucleic acid-induced levels of IL-6 were low compared to those induced by A. pleuropneumoniae. The IFN-alpha response elicited by pcDNA3 in the presence of lipofectin was as high as, or higher than that induced by ADV. Interestingly, also A. pleuropneumoniae induced a substantial production of IFN-alpha when preincubated with lipofectin. Plasmid expression was not necessary for induction of IFN-alpha. Furthermore, the IFN-alpha inducing capacity of pcDNA3 was not reduced when the two predicted immunostimulatory sequences 5'AACGTT3' were deleted. Nor did the ability of the plasmid to induce IFN-alpha production decrease when the ampicillin resistance (ampR) gene was replaced with the kanamycin resistance (kanR) gene. However, methylation of all cytidines in CpG dinucleotides of pcDNA3 abolished the IFN-alpha inducing capacity. These in vitro results indicate an immunomodulatory role of bacterial DNA also in the pig. Unmethylated CpG dinucleotides are crucial for induction of IFN-alpha by the plasmid, but other CpG motifs than those within the 5'AACGTT3' sequences of the ampR gene contribute to this induction in porcine cells.  相似文献   

Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects   总被引：1，自引：0，他引：1  

Johansson AM  Gardner SY  Levine JF  Papich MG  LaFevers DH  Fuquay LR  Reagan VH  Atkins CE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):887-895

Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

Variability associated with the official USDA sampling plan used to inspect export wheat shipments for Tilletia controversa spores     

T. B. Whitaker †  J. Wu  G. L. Peterson  F. G. Giesbrecht  A. S. Johansson 《Plant pathology》2001,50(6):755-760

The variability associated with estimating the true concentration of teliospores of dwarf bunt ( Tilletia controversa ) per 50 g of wheat (TC concentration) in an export wheat shipment was studied by measuring the TC concentration in 16 test samples (50 g) taken from each of 137 export shipments. The variability among the 16 TC test sample results, as measured by the standard deviation, was found to increase with TC concentration. The functional relationship was approximately linear in a full-log plot and regression analysis was used to determine the coefficients of the regression equation. Using statistical theory, the regression equation was modified to predict the standard deviation among test sample sizes other than the 50 g size used in this study. The standard deviation and coefficient of variation associated with using a 50 g test sample to estimate the true TC concentration of a wheat shipment with 2000 spores per 50 g were estimated to be 1062·8 and 53·1%, respectively. Increasing test sample size to 1600 g reduced the standard deviation and coefficient of variation to 187·9 and 9·4%, respectively.  相似文献   

Effect of SC58125 on cell proliferation and apoptosis in HepG-2 cells     

KE Xiao-long  LI Hong-liang  CHEN Dan-dan  REN Xian-da  ZHANG Hai-wei  CHEN Xiao-dong  XIONG Ai-hua 《园艺学报》2002,18(1):51-54

AIM: To clarify the effect of SC58125 on cell proliferation and apoptosis in HepG-2 cells and explore the molecular mechanisms. METHODS: Cell culture, MTT, TUNEL, DNA ladder, flow cytometry and Western blot analysis were employed in the present study. RESULTS: SC58125 inhibited the growth of HepG-2 cells and induced the apoptosis. Furthermore, it arrested G₀/G₁ phase and inhibited S phase in HepG-2 cells. Depressed expression of P33^cdk2, P34^cdc2, cyclin B₁, cyclin E, Mpm-2, Rb, PCNA proteins were found in HepG-2 cells treated with SC58125. CONCLUSION: SC58125 inhibits cell proliferation and induces apoptosis, which may be related to the altered low protein levels of P33^cdk2, P34^cdc2, cyclin B₁, cyclin E, Mpm-2, Rb, PCNA.  相似文献   

Effect of stress on myocardial cells and blood levels of catecholamines in normal and amygdalectomized pigs.   总被引：1，自引：1，他引：0       下载免费PDF全文

G Johansson  K Olsson  J Hggendal  L Jnsson    K Thorn-Tolling 《Canadian journal of veterinary research》1982,46(2):176-182

The effects of restraint stress on heart activity, blood catecholamines and the development of myocardial necrosis were studied in five unoperated and five unsuccessfully amygdalectomized (sham-operated) pigs and compared with six bilaterally amygdalectomized pigs. In the sham-operated and the nonoperated animals, restraint stress often provoked intense tachycardia, as well as considerable elevations of blood catecholamines, particularly noradrenaline, associated with more or less widespread foci of myocardial necrosis. In contrast, the same restraint produced only minor heart rate increase in the six completely amygdalectomized animals; their blood noradrenaline levels were significantly lower and no myocardial necrosis could be detected, either macro- or microscopically. The results indicate that the amygdaloid part of the limbic system and the sympathoadrenal system are of importance in the induction of myocardial necrosis often noted in pigs when exposed to situations of severe mental stress.  相似文献   

Isolation of Mycoplasma columborale from a fly (Musca domestica)     

Kempf I  Chastel C  Ferris S  Dufour-Gesbert F  Johansson KE  Pettersson B  Blanchard A 《The Veterinary record》2000,147(11):304-305

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Pharmacokinetics and pharmacodynamics of terbutaline in healthy horses     

Törneke MK  Ingvast-Larsson JC  Johansson JM  Appelgren LE 《American journal of veterinary research》2000,61(7):761-765

OBJECTIVE: To determine pharmacokinetics of terbutaline in healthy horses and to relate serum terbutaline concentrations with the drug's pharmacodynamic effects. ANIMALS: 6 healthy horses. PROCEDURE: Horses were given terbutaline i.v. (10 microg/kg of body weight) and, 1 week later, p.o. (100 microg/kg). Responses to drug administration (eg, heart rate and serum lactate concentration) were measured. Serum terbutaline concentration was measured by means of gas chromatography with mass spectrometry. Protein binding was determined in vitro. RESULTS: Following i.v. administration, median maximum serum terbutaline concentration and mean residence time were 9.3 ng/ml and 30 minutes, respectively. Bioavailability following oral administration was < 1%. All horses developed sweating, trembling, excitement, and tachycardia during i.v. infusion. The 2 horses with the highest serum terbutaline concentrations developed severe tachycardia and CNS stimulation; 30 minutes after the i.v. infusion was completed, they were hyperventilating and lethargic. Heart rate and serum lactate concentration increased as serum terbutaline concentration increased. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that terbutaline is rapidly cleared from the bloodstream following i.v. administration to horses, suggesting that continuous i.v. infusion would be needed to maintain therapeutic serum concentrations. Oral administration of terbutaline to horses is not practical because of the low bioavailability.  相似文献   

花臭蛙皮肤分泌物中抗菌肽Odorranin的分离纯化与表征   总被引：1，自引：0，他引：1  

周建武  李珑  柯李晶  高观桢  饶平凡 《中国兽药杂志》2007,41(12):20-23

从花臭蛙(Odorrana schmackeri)皮肤分泌液中分离得到一类(包含三种)新型的抗菌肽.经过一级结构解析和抗菌活性表征后,命名为Odorranin(1,2,3).该类抗菌肽由21个氨基酸组成.Odorranin对大肠杆菌和金黄色葡萄球菌具有明显的抑制活性,其中Odorranin 3对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度分别为4.2 、6.06 μg/mL.  相似文献   

Effect of chronic cigarette smoking on BK_Ca and Kv1.5 expression in rat pulmonary arterial smooth muscle cells     

YE Hong  JIN Si  YE Shi-qiao  DENG Shi-wei  KE Dan  HU Qing-hua  LIU Sheng-yuan  WANG Di-xun 《园艺学报》2007,23(2):262-265

AIM:To investigate the role of potassium channel expression alteration in chronic cigarette smoking-induced increase in pulmonary vascular responsiveness, the effect of chronic cigarette smoking on large-conductance calcium-activated potassium channel (BK_Ca) and voltage-dependent delayed rectifier potassium channel (Kv1.5) expression in rat pulmonary smooth muscle cells were investigated in vivo. METHODS:HE staining, immuno-histochemistry and in situ hybridization techniques were used. RESULTS: (1) Chronic cigarette smoking downregulates the protein and mRNA expression of BK_Ca in pulmonary arterial smooth muscles. (2) Chronic cigarette smoking downregulated the protein and mRNA expression of Kv1.5 in pulmonary arterial smooth muscles. (3) In big artery, BK_Ca decreased more makedly than Kv1.5, but in small artery, both of them decreased equally. CONCLUSION:Chronic cigarette smoking downregulates the levels of BK_Ca and Kv1.5 in rat pulmonary arterial smooth muscle cells in vivo, which maybe contribute to the mechanism of cigarette smoking-induced increase in pulmonary vascular responsiveness.  相似文献