全文获取类型
收费全文 | 8170篇 |
免费 | 416篇 |
国内免费 | 9篇 |
专业分类
林业 | 588篇 |
农学 | 275篇 |
基础科学 | 62篇 |
1386篇 | |
综合类 | 1234篇 |
农作物 | 517篇 |
水产渔业 | 647篇 |
畜牧兽医 | 3018篇 |
园艺 | 215篇 |
植物保护 | 653篇 |
出版年
2023年 | 46篇 |
2022年 | 58篇 |
2021年 | 137篇 |
2020年 | 151篇 |
2019年 | 174篇 |
2018年 | 154篇 |
2017年 | 148篇 |
2016年 | 172篇 |
2015年 | 130篇 |
2014年 | 183篇 |
2013年 | 342篇 |
2012年 | 408篇 |
2011年 | 562篇 |
2010年 | 319篇 |
2009年 | 298篇 |
2008年 | 548篇 |
2007年 | 502篇 |
2006年 | 511篇 |
2005年 | 539篇 |
2004年 | 442篇 |
2003年 | 481篇 |
2002年 | 420篇 |
2001年 | 93篇 |
2000年 | 88篇 |
1999年 | 100篇 |
1998年 | 85篇 |
1997年 | 76篇 |
1996年 | 67篇 |
1995年 | 89篇 |
1994年 | 86篇 |
1993年 | 73篇 |
1992年 | 57篇 |
1991年 | 54篇 |
1990年 | 64篇 |
1989年 | 45篇 |
1988年 | 41篇 |
1987年 | 36篇 |
1986年 | 48篇 |
1985年 | 51篇 |
1984年 | 49篇 |
1983年 | 46篇 |
1982年 | 32篇 |
1981年 | 25篇 |
1980年 | 34篇 |
1979年 | 29篇 |
1978年 | 37篇 |
1977年 | 37篇 |
1976年 | 35篇 |
1975年 | 38篇 |
1974年 | 30篇 |
排序方式: 共有8595条查询结果,搜索用时 484 毫秒
991.
Connor EE Cates EA Williams JL Bannerman DD 《Veterinary immunology and immunopathology》2006,112(3-4):302-308
Toll-like receptor (TLR)-4 is a transmembrane receptor for lipopolysaccharide, a highly pro-inflammatory component of the outer membrane of Gram-negative bacteria. To date, molecules of the TLR-4 signaling pathway have not been well characterized in cattle. The goal of this study was to clone and sequence the full-length coding regions of bovine genes involved in TLR-4 signaling including CASP8, IRAK1, LY96 (MD-2), TICAM2, TIRAP, TOLLIP and TRAF 6 and to position these genes, as well as MyD88 and TICAM1, on the bovine genome using radiation hybrid mapping. Results of this work indicate differences with a previously published bovine sequence for LY96 and a predicted sequence in the GenBank database for TIRAP based on the most recent assembly of the bovine genome. In addition, discrepancies between actual and predicted chromosomal map positions based on the Btau_2.0 genome assembly release were identified, although map positions were consistent with predicted locations based on the current bovine-human comparative map. Alignment of the bovine amino acid sequences with human and murine sequences showed a broad range in conservation, from 52 to 93%. Overall, this work should assist in the assembly and annotation of the bovine genome sequence, the identification of variations in genes critically involved in host innate immunity, and facilitate the study of TLR-4 signaling pathways in cattle. 相似文献
992.
Scrapie is a natural transmissible spongiform encephalopathy (TSE) of sheep, infecting the animal via the gastrointestinal tract or the skin. This project tested the hypotheses that lymph-borne cells (especially dendritic cells) are crucial for the systemic dissemination of the infectious agent from the site of infection in the skin, that PrP genotype affects PrPSC association with dendritic cells and that PrPSC carriage by cells affects their expression of cytokines. Skin, of scrapie-susceptible VRQ/ARR and scrapie-resistant ARR/ARR PrP genotypes, was scarified with FITC-labelled PrPSC. Pseudoafferent lymphatic cannulation was then used to monitor the presence of FITC-PrPSC over time in different lymph cell populations and plasma in the draining afferent lymphatics. The major observation was that PrPSC did not associate significantly with any lymphocyte or dendritic cell population in the 5 days following PrPSC scarification. The only cells seen to associate with PrPSC were neutrophils. Furthermore, despite the quantity of PrPSC used for scarification being equivalent to a standard infectious dose (the VRQ/ARR sheep dying at approximately 260 days) the only PrP found in afferent lymph during the 0-5-day period was proteinase K sensitive (i.e. soluble PrPC). No differences were observed between the PrP genotypes. Analysis of the effects of PrPSC scarification of cellular cytokine mRNA expression (by a nuclease protection assay) showed raised levels of IL-1beta and IL-8 in the susceptible VRQ/ARR group and raised levels of IFNgamma in the resistant ARR/ARR animals. 相似文献
993.
Collins JD 《Veterinary microbiology》2006,112(2-4):369-381
In the later stages of eradication of tuberculosis in cattle there is a need to take account of the fact that Mycobacterium bovis infection in cattle presents, not as cases of clinical disease but most commonly as apparently healthy animals showing an immunological response to tuberculin. This is an entirely different scenario to that seen when national eradication programmes were first devised, at a time when the protection of public health rather than animal health was the prime motivation. In countries with active programmes to eradicate bovine tuberculosis, it is critical for the programme's success that account is taken of this redefinition of tuberculosis, side by side with changes in modern animal production systems and their impact on the transmission of M. bovis. This paper highlights factors critical to the success of a national eradication programme, including a clear identification of the goals, of the policies that guide actions, and of the sequences of actions that are required within the programme to accomplish these goals. Experience has illustrated the adverse effects of compromise on outcome when the application of fundamental principles of disease control such as sound animal management, removal of known sources of infection, early diagnosis, quarantine, movement control and environmental hygiene are less than enthusiastically promoted and applied. The reality is that where these principles are applied in a sustained manner, the outcome is more likely to be successful. Therein lies the challenge for the risk manager. 相似文献
994.
Occurrence of methicillin-resistant Staphylococcus aureus strains from cattle and chicken, and analyses of their mecA, mecR1 and mecI genes 总被引:2,自引:0,他引:2
Lee JH 《Veterinary microbiology》2006,114(1-2):155-159
From 2001 to 2005, various specimens from cattle, pigs, and chickens were collected and examined for the presence of methicillin (oxacillin)-resistant Staphylococcus aureus (MRSA). The isolates from 19 specimens were tested for the presence of the mecA gene. Methicillin resistance was confirmed by determining the MICs for these isolates. Among these 19 mecA-positive isolates, 16 were consistently found to be resistant to methicillin. The mecR1 gene was found in all 19 mecA-positive S. aureus, and mecI was also detected in 15 of the mecA-positive S. aureus. The mecI gene had an identical sequence to the reference sequence in 9 of the 15 mecI-positive isolates. Three of the other six isolates had a C to T substitution at nucleotide 202, and one had a G to T substitution at nucleotide 43. These have been previously identified in MRSA from humans. Two isolates from chickens contained an addition of C at position 23. This mutation of MRSA has not been reported elsewhere. In all 15 mecI-positive MRSA, the sequence of the mec promoter/operator region was identical to the reference sequence. This suggests other mechanisms for overcoming the repression of resistance caused by mecI, beyond the simple product interaction between the mecA, mecRI, and mecI genes. 相似文献
995.
Stoneham AE Graham J Rozanski EA Rush JE 《Journal of the American Animal Hospital Association》2006,42(6):457-461
A 4-year-old, late-term pregnant Himalayan queen was diagnosed with biventricular congestive heart failure characterized by pleural effusion and pulmonary edema. A cesarean section was performed, resulting in the birth of four live kittens, with one surviving to discharge. Congestive heart failure resolved rapidly following surgery. The queen remained asymptomatic for 9 years postoperatively. Volume expansion secondary to pregnancy was thought to result in cardiovascular volume overload and subsequent congestive heart failure. 相似文献
996.
Paczoska-Eliasiewicz HE Proszkowiec-Weglarz M Proudman J Jacek T Mika M Sechman A Rzasa J Gertler A 《Domestic animal endocrinology》2006,31(3):211-226
The present study was undertaken to examine the effect of recombinant chicken leptin administered to fed ad libitum and feed-restricted immature chickens of a layer strain on ovarian development and the timing of sexual maturity. In the first experiment 11-week-old pullets (77 days of age) fed ad libitum were injected daily with leptin at four dose levels (4, 16, 64 and 256 microg/kg body weight) until sexual maturity (lay of the first egg). Leptin treatment at the highest dose significantly (P<0.05) advanced the onset of puberty (day 116.3+/-1.0) in comparison to controls (day 121.3+/-1.2). The rises of luteinizing hormone, estradiol and progesterone in blood plasma were also advanced by leptin treatment. In the second experiment, both full-fed and feed-restricted pullets (79 days of age) were injected daily with leptin (256 microg/kg body weight). In birds fed ad libitum, exogenous leptin again significantly (P<0.05) advanced first ovipostion (day 118.4+/-1.4 versus day 124.4+/-1.7), while abolishing the significant (P<0.05) delay caused by feed restriction (day 131.5+/-1.6) and restoring the normal onset of sexual maturity (day 125.7+/-1.6). Analysis of the ovaries in 106-day-old pullets revealed that leptin injections advanced follicular development, particularly in birds fed ad libitum, and significantly (P<0.01) reduced follicular apoptosis both in full-fed and feed-restricted birds. In conclusion, we have shown that in female chickens exogenous leptin advances the onset of puberty by attenuation of ovarian apoptosis and enhancement of folliculogenesis. 相似文献
997.
998.
999.
It has been suggested that dental abnormalities lead to temporomandibular joint inflammation and pain that may be mitigated by regular dental care. There is considerable literature on the pathophysiology of equine joint disease including studies on cytokine profiles in diseased appendicular joints. This study examined the effects of age and dental malocclusions summarized as a dental pathology score on equine temporomandibular joint cytokine (IL-1, IL-6, IL-8, TNF alpha and TGF-beta1, -beta2, -beta3) concentrations. TGF-beta3 was not detected in any joint sample. IL-1, IL-6 and TNF alpha were not influenced by age. Foals had significantly lower concentrations of lL-8 and TGF-beta1, and higher levels of TGF-beta2 compared with older horses. Age did not effect cytokine concentration in older horses although there was a trend towards increasing 1L-8 with age. The dental pathology score increased with age in mature horses, however there was no effect of dental pathology score on cytokine concentration. There was no effect of incisor eruption, and presence or number of periodontal lesions on temporomandibular joint cytokine concentration. Our findings indicate that age but not dental pathology affected temporomandibular joint proinflammatory cytokine concentration in this population of horses. 相似文献
1000.
Bach JF Rozanski EA MacGregor J Betkowski JM Rush JE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(5):1132-1135
Pulmonary arterial hypertension (PH) is a pathologic condition in dogs characterized by abnormally high pressures in the pulmonary circulation and has been associated with a poor outcome. Sildenafil is a type V phosphodiesterase inhibitor that produces nitric oxide mediated vasodilatation. Sildenafil treatment decreases pulmonary arterial pressure and pulmonary vascular resistance in people with PH. The purpose of this study was to describe the clinical characteristics and outcome of dogs with PH treated with sildenafil. The cardiology database was searched for dogs with PH treated with sildenafil. PH was defined as systolic pulmonary arterial pressure (PAPs) > or = 25 mmHg at rest. Medical records were reviewed for the following information: signalment, duration and type of clinical signs before treatment, underlying disease, estimated or measured PAPs, dosage and dosing interval of sildenafil, and the effect of treatment on clinical signs and pulmonary arterial pressure and survival time. Thirteen affected dogs were identified. Clinical signs included collapse, syncope, respiratory distress, and cough. Duration of clinical signs before presentation ranged from 3 days to 5 months. An underlying cause was identified in 8 dogs. The median sildenafil dosage was 1.9 mg/kg. Ten dogs received concurrent medications. Median PAPs was 90 mmHg; 8 dogs were reevaluated after therapy, and the median decrease in PAPs was 16.5 mmHg. The median survival time of all dogs was 91 days. Sildenafil appeared to be well tolerated in dogs with PH and was associated with decreased PAPs and amelioration of clinical signs in most. Sildenafil represents a reasonable treatment option for dogs with pulmonary hypertension. 相似文献