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61.
Megan Palmisano Susan Bender Amy L. Johnson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):361-365
We describe the unique clinical presentation of a central nervous system neoplasm in a 6-month-old draft horse cross gelding. Based on the neurologic examination at admission, neurolocalization was most consistent with a mildly asymmetric cervical, multifocal, or diffuse myelopathy. Mild vestibular involvement also was considered, but no cranial nerve deficits were observed. The gelding was negative for Sarcocystis neurona or Neospora hughesi based on paired serum and cerebrospinal fluid (CSF) samples analyzed, with no evidence of cervical compression based on contrast myelography. The horse was euthanized because of progression of clinical signs. At necropsy, a mass was identified associated with the cerebellum, and histopathology was consistent with medulloblastoma, which has not been reported previously in the horse. 相似文献
62.
Kara A. Brown Amy L. Johnson Susan J. Bender Thomas J. Nolan Bianca Pfisterer JoAnn Slack Elinor Willis Emily K. Rule 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):735-739
A 5-year-old imported Zangersheide gelding was evaluated for SC swellings over both forelimbs and lameness localized to the distal metacarpus. Ultrasound examination of the SC masses was compatible with verminous granulomas. Linear hyperechoic foci were present within the suspensory ligament branches of both forelimbs, suggestive of ligamentous parasitic infiltrates. A diagnosis of onchocerciasis was confirmed on biopsy of a SC mass. The gelding was treated with ivermectin and a tapering course of PO dexamethasone but was eventually euthanized. Necropsy confirmed the presence of SC eosinophilic granulomas and degenerative suspensory ligament desmitis, both with intralesional nematodes. Given the location and appearance of the nematode, a diagnosis of Onchocerca sp., most likely O. reticulata, was made. Onchocerciasis should be included as a differential diagnosis for multifocal suspensory ligament desmitis with these sonographic characteristics when paired with SC masses in imported European Warmbloods. 相似文献
63.
The mobility of alachlor from alginate-encapsulated controlled-release (CR) formulations was investigated in two contrasting soil profiles. Two CR formulations of alachlor were prepared with the following components (1) base—sodium alginate+kaolin+‘Tween’ 20 (1+10+0·5 by mass) and (2) base+40 g kg−1 linseed oil. These were compared to technical grade alachlor and to a commercial alachlor EC formulation (‘Lasso’ 4EC). All herbicide treatments were labeled with [14C]alachlor and were applied to duplicate soil columns that were composed of a surface and a subsoil horizon. Each horizon was packed to a depth of 12·5 cm, giving a total column length of 25 cm. The columns were leached with 21 cm (420 ml) to 30 cm (600 ml) of 0·01M calcium chloride for a period of 7 to 10 days. Alachlor leaching from the EC formulations was the same as that from the technical material in both soils: 33% in the Evesboro and 10% in the Conover soil. The CR-Oil formulation leached 4 and 2% of the applied [14C]alachlor, compared to 12 and 3% for the CR-N formulation for the Evesboro and Conover soils, respectively. The CR-Oil formulation also increased the amount of [14C]alachlor retained in the soil surface horizon (105–114%), compared to CR-N (39–45%), technical material (14–23%) and EC (12–17%). 相似文献
64.
Johnson Shelby L. Park Hyun Y. Vattem Dhiraj A. Grammas Paula Ma Hang Seeram Navindra P. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):512-517
Plant Foods for Human Nutrition - Emerging data support that plant food based isoflavones have ameliorating effects on a variety of neurodegenerative diseases including Parkinson’s disease... 相似文献
65.
动物精子的性别可通过流式精子分选仪和DNA标识进行鉴定,而利用鉴定性别的精子(即性控精子)并借助人工授精技术或其它授精技术产生的后代,在过去5年中估计已多达30000个(其中大多数是母牛)。有关文献资料证明,能有效地区分X精子和Y精子的唯一标记物是精子染色体中的DNA。众所周知,目前世界各地采用的方法是Beltsville精子分选技术,该技术根据X精子群和Y精子群中DNA相对含量上的差异,用荧光染液(Hoechst33342染液)标记精子,随后利用流式细胞仪分选经荧光标记的精子,从而达到分离精子的目的。目前,X精子或Y精子正常的生产速度是每小时1500万个,该项技术已在家畜、实验动物和动物园动物中应用,如果将该技术应用在人上,在预选后代性别比例上可达90%~95%的成功率。因动物品种不同,性控精子在动物体内的授精部位也不相同。常规的人工授精技术、宫内授精技术、输卵管内授精技术、用于胚胎移植的体外受精(in-vitro fertilization,IVF)技术或子宫角深部授精技术均能有效地使动物怀孕,至于利用哪种技术进行性控精子的精则取决于动物品种。尽管所有动物都能获得高纯度的分选精子,但是在实际生产中利用低剂量精子还难以让母猪怀孕。子宫角深部授精技术每次授精0.5~1.00亿个性控精子已能产生可喜的效果:利用特制的输精管,输入常规人工授精所需精子量的五十分之一的性控精子,足以使动物怀孕。性控精子通过常规的授精技术能够被猪成功利用前,还需重新设计输精管,同时输精的次数和每次授精时精子数量也需作进一步的研究。分选精子的低温保存技术已被牛人工授精普遍采用。尽管已能利用冷冻的性别分选精子生产小猪,但是性别分选精子经冷冻和解冻处理后在常规生产中的应用还未达到最理想的效果。本文将讨论猪精子性别分选的最新研究成果及其发展趋势,并重点探讨将性别分选技术应用于养猪生产中必须对其进行必要的技术开发。 相似文献
66.
Oswaldo A. Rubio-Covarrubias Patrick H. Brown Steven A. Weinbaum R. Scott Johnson Raul I. Cabrera 《Scientia Horticulturae》2009
Mobile nitrogen (N) forms may be better N indicators of the N status of trees than total nitrogen (TN) due to their higher sensitivity to increasing N supply. A field experiment was carried out over a 3-year period to compare foliar concentrations of total N (TN), soluble N (SN), chlorophyll (Minolta SPAD readings), NH4–N and NO3–N as indicators of soil N availability in nectarine, Prunus persica L. Batsch, cv. ‘Fantasia’ (grafted on ‘Nemaguard’ peach, P. persica × P. davidiana) trees. Young trees were exposed to a range of fertilizer-N application rates. Based on correlation analysis, the best association between leaf N compounds with soil N supply and trunk diameter and/or fruit yield was obtained with TN and chlorophyll SPAD readings. Leaf concentrations of mobile N compounds (NH4–N and NO3–N) increased more than any other N compound under high N supply; however, their inconsistency among years and low leaf concentration difficult their use as N indicators. The optimum foliar TN for growth decreased with tree age, 4.4%, 3.6% and 3.3% in non-bearing 1-year-old trees, non-bearing 2-year-old trees and 3.3 fruit-bearing 3-year-old trees. The optimum SPAD readings were 40 in 2-year-old trees and 42 in 3-year-old trees. Stable N compounds (TN and chlorophyll SPAD) could be used to N diagnosis in the zone of N deficiency, and soluble N compounds (NH4–N and NO3–N) to diagnoses N excess. 相似文献
67.
There is debate among ecologists about whether total habitat area or patch arrangement contributes most to population and/or
community responses to fragmented or patchy landscapes. We tested the relative effects of patch area and isolation for predicting
bird occurrence in a naturally patchy landscape in the Bear River Mountains of Northern Utah, USA. We selected focal patches
(mountain meadows) ranging in elevation from 1,920 to 2,860 m and in size from 0.6 to 182 ha. Breeding birds were sampled
in each focal meadow during the summers of 2003 and 2004 using variable-distance point transects. Logistic regression and
likelihood-based model selection were used to determine the relationship between likelihood of occurrence of three bird species
(Brewer’s sparrow, vesper sparrow, and white-crowned sparrow) and area, isolation, and proximity metrics. We used model weights
and model-averaged confidence intervals to assess the importance of each predictor variable. Plots of area versus isolation
were used to evaluate complex relationships between the variables. We found that meadow area was the most important variable
for explaining occurrence for two species, and that isolation was the most important for the other. We also found that the
absolute distance was more appropriate for evaluating isolation responses than was the species-specific proximity metric.
Our findings add clarity to the debate between ecologists regarding the relative importance of area and isolation in species
responses to patchy landscapes. 相似文献
68.
关于硬骨鱼类的嗅、味感觉器官在解剖学和生理学方面的研究成果,许多学者都发表过综述文章(Kleerekoper1969,Bardach和Atema1971,Bardach和Villars1974,Kapoor等1975,Brown和Hara1982,Tucker1983),最近十几年,对于鱼类化学感觉的电生理研究,主要在于一些天然物质、氨基酸及其衍生物对于嗅、味觉系统的相对刺激效果,刺激物的分子结构与刺激效果的关系,以及刺激物与感受细胞膜上的感受点相互作用的机制,而对于人为因素如捕捞、环境因素pH、污染对鱼类嗅、味觉的影响报导甚少(Hara1976,1978,Konishi等1969,Tucker1973,Silver,1978,Cancolon1980,Hidaka1970)。鱼类的嗅、味觉在鱼类生命活动如摄食、洄游、生殖、逃避敌害、群体的控制等等行为中起重要的作用,当人们利用鱼类嗅、味觉的一些生理活动规律为生产实践服务时,pH是不可忽视的一个因素,本试验以重要的生物信息分子氨基酸为刺激物,研究pH对于鱼类嗅、味觉系统对氨基酸敏感性的影响。 相似文献
69.
R. W. Mullen S. B. Phillips W. R. Raun G. V. Johnson W.E. Thomason 《Journal of plant nutrition》2013,36(5):673-681
Bermudagrass (Cynodon dactylon L.) is a warm season perennial that is well adapted in the southern Great Plains. It is one of the region's most important forage crops used for livestock production, and is commonly grown without legume interseeding. Recent research has investigated ways of improving the quality and quantity of this forage. The objectives of this study were to determine the effect of interseeded legumes and phosphorus (P) fertilizer on bermudagrass pasture forage yield and crude protein content. One experiment was initiated in 1993 in eastern Oklahoma in an established bermudagrass pasture. Red clover (Trifolium pratense L.), ladino clover (Trifolium repens L.), and two varieties of alfalfa (Medicago sativah), ’alfagraze’ and'common’, were interseeded by hand into an established stand of bermudagrass. The effect of P on forage yield and crude protein was evaluated using a 30‐kg P ha‐1 rate applied at establishment versus no applied P. Forage yield was collected three times throughout the growing season each year from 1994 through 1997. When both alfalfa varieties were interseeded into a bermudagrass pasture without applying additional P fertilizer, forage yields for the legume‐grass mixtures decreased below those obtained from the monoculture bermudagrass in the first year of the stand. The alfalfa variety ‘alfagraze’ interseeded into established bermudagrass decreased total forage yield over the entire 4‐yr study. Interseeded red clover and ladino clover increased crude protein of the forage compared with monoculture bermudagrass the first two years of the study, with red clover continuing to increase crude protein in the fourth year. However, when 30 kg P ha‐1 was applied to the bermudagrass prior to establishment of the legumes, no change in yield or protein was observed for both alfalfa varieties’ interseeding treatments versus the unfertilized mixtures. Although forage yield may not be increased, interseeding legumes into established bermudagrass could provide an efficient way to improve pasture crude protein without the use of inorganic fertilizers. However, if alfalfa ('common’ or ‘alfagraze') is interseeded, additional P may need to be applied at legume establishment to prevent possible yield decreases. 相似文献
70.
Shannon L. Taylor William R. Raun John B. Solie Gordon V. Johnson Marvin L. Stone Richard W. Whitney 《Journal of plant nutrition》2013,36(11):2287-2302
The use of variable rate technology has become increasingly popular for applying plant nutrient elements. The most widely used method for determining variable fertilizer rates is presently based on soil testing and yield mapping. Three field studies (Bumeyville 1995, Burneyville 1996, and Ardmore 1996) were initiated in established Midland bermudagrass [Cynodon dacrylon (L) Pers.] pastures to determine the relationship between spectral radiance at specific wavelengths with forage nitrogen (N) removal and biomass, and to determine field variability of soil test parameters. Variable N (applied to 1.5 × 2.4 m subplots within 2.4 × 45.7 m main plots), fixed N and check treatments were evaluated at each location. Spectral radiance readings were taken in the red (671±6 nm), green (570±6 nm), and near infrared (NIR) (780±6 nm) wavelengths. The normalized difference vegetation index (NDVI) was calculated as NIR‐red/NIR+red. Variable N rates were applied based on NDVI. The highest fixed variable N rate was set at 224, 336, and 672 kg N ha‐1 for Burneyville, 1995, 1996, and Ardmore, 1996, respectively. At Bumeyville, soil samples were collected in all variable rate plots (1.5 × 2.4 m) and analyzed for various soil test characteristics. NDVI, red, green, and NIR spectral radiance readings were correlated with bermudagrass forage N removal and yield. Correlation of forage yield and N removal with red, NIR, and NDVI were best with maximum forage production, however, when forage production levels were low correlation decreased dramatically for the red wavelength compared with NIR and NDVI. Forage yield and forage N removal in variable rate treatments increased when compared to the check while being equal to the half‐fixed and fixed rates where higher N rates were applied. Also, variability about the mean in variable rate plots was significantly lower than half‐fixed and fixed rates which supports adjusting N rates based on indirect NDVI measurements. Variable N rate plots reduced fertilizer inputs by 60% and produced the same yield as fixed rate plots, while fixed and half‐fixed rates did not increase N content in the forage over that of the variable rate treatment. Soil sample data collected from small consecutive plots (<4 m2) was extremely variable indicating that intense sampling would be needed if variable fertilizer application were to be based on soil test results. 相似文献