Prevalence of megabacteria in budgerigar colonies     

LJ FILIPPICH  JK HENDRIKZ 《Australian veterinary journal》1998,76(2):92-95

Objective To measure the prevalence of megabacteria in budgerigar-breeding colonies and to evaluate possible methods to reduce the prevalence.
Design A monitoring study over several years.
Sample population Two budgerigar ( Melopsittacus undulatus ) colonies with over 300 birds each.
Procedure The prevalence of megabacteria in the faeces in two budgerigar breeding colonies, colony 1 and 2, was determined by faecal examination of each bird. Following an initial survey (1990), most of the birds that were scored 2+ or more were culled and a management practice was implemented to discriminate against positive birds. Consecutive yearly surveys (1991, 1992) were conducted on the young birds bred in these colonies. The prevalence of megabacteria in colony 2 was also evaluated in 1994 and 1996 after all the birds were treated with amphotericin B administered in drinking water.
Results The prevalence of megabacteria in the two colonies was significantly (P < 0.001) different. Overall the prevalence of megabacteria adjusted for colony differences was significantly higher (P < 0.025) in males compared to females. Age was not an influencing factor. After the initial survey, the prevalence in the offspring did not significantly (P > 0.05) decrease in the following two annual breeding seasons but by inference it did significantly decrease after amphotericin B treatment.
Conclusion The practice of culling most birds with more megabacteria in faeces and discriminating against positive birds when selecting birds for breeding or culling birds on show quality does not decrease megabacteria prevalence in the offspring. However, a reduction in prevalence does occur with administration of amphotericin B. Birds may have amphotericin B-resistant organisms and these birds need to be identified and culled.  相似文献   

The Feline Patient, Essentials of Diagnosis and Treatment     

JK Blackshaw 《Australian veterinary journal》1998,76(12):827-827

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Physiological and storage problems number     

H. S. Yohe  Wm. Stuart  C. O. Appleman  John Bushnell  Julian A. Dimock  J. T. Rosa  Earl S. Johnston  E. P. Miller  Freeman Weiss 《American Journal of Potato Research》1925,2(9):343-382

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Effects of sample handling on adrenocorticotropin concentration measured in canine plasma, using a commercially available radioimmunoassay kit.   总被引：1，自引：0，他引：1  

R L Hegstad  S D Johnston  D M Pasternak 《American journal of veterinary research》1990,51(12):1941-1947

A commercially available radioimmunoassay (RIA) kit for measurement of human adrenocorticotropin (hACTH) was validated for use in dogs. Assay sensitivity was 3 pg/ml. Intra-assay coefficient of variation (x 100; CV) for 3 canine plasma pools was 3.0 (mean +/- SD, 33 +/- 0.99 pg/ml), 4.2 (71 +/- 2.4 pg/ml) and 3.7 (145 +/- 3.7 pg/ml) %. Interassay CV for 2 plasma pools measured in 6 assays was 9.8 (37 +/- 3.6 pg/ml) and 4.4 (76 +/- 3.4 pg/ml) %, respectively. Dilutional parallelism was documented by assaying 2 pools of canine plasma at 3 dilutions and correcting the measured result for dilution. Corrected mean concentrations for the first pool were 33 (+/- 0.99), 36 (+/- 4.3), and 33 (+/- 6.8) pg/ml; corrected mean concentrations for the second pool were 145 (+/- 5.4), 141 (+/- 10.8) and 125 (+/- 3.4) pg/ml. Recovery of 1-39hACTH added to canine plasma (6.25, 12.5, 25.0, 50.0, and 100.0 pg/ml) was linear and quantitative (slope = 0.890, R2 = 0.961). To test whether anticoagulant or the protease inhibitor, aprotinin, influences ACTH concentration in canine plasma, ACTH was measured in canine blood collected in 4 tubes containing anticoagulant: heparin (H), heparin + 500 kallikrein inhibitor units (KIU) of aprotinin/ml (HA), EDTA (E), and EDTA + aprotinin (EA). Plasma ACTH concentration was the same when samples containing H and HA, or HA and E were compared, and was significantly (P less than 0.01) lower in samples containing EA.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Effects of intravesical hydrostatic pressure and volume on the distensibility of the canine prostatic portion of the urethra     

G R Johnston  D A Feeney  C A Osborne  S D Johnston  F O Smith  C R Jessen 《American journal of veterinary research》1985,46(3):748-751

Positive-contrast retrograde urethrocystograms were obtained serially on 12 male dogs weighing 11.4 to 23.2 kg before, during, and after the injection of contrast medium until the urinary bladder neck and prostatic and membranous portions of the urethra remained open and distended as viewed by fluoroscopy. Correlations of intravesical volumes and pressures required to achieve maximum distension of the midprostatic portion of the urethra with body weight and surface area were not significant. Because of the variability in intravesical volumes and pressures encountered at maximum distension of the prostatic portion of the urethra, a dose of contrast material expressed relative to body weight or surface area could not be determined for consistently providing maximum distension of the prostatic portion of the urethra.  相似文献   

Ex Vivo Evaluation of Carpal Flexion After Partial Carpal Arthrodesis in Horses          下载免费PDF全文

Patty J. Tulloch DVM  James D. Johnston PhD  Spencer M. Barber DVM  Diplomate ACVS  Candace L. Gellert BSc  Hayley M. Lang DVM  Luca Panizzi DrMedVet  MVetSc  Diplomate ACVS‐LA 《Veterinary surgery : VS》2015,44(3):386-391

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Volatile fatty acid profile for grass hay or alfalfa hay fed to alpacas (Vicugna pacos)     

C. L. Oldham  T. F. Robinson  Z. R. Hunter  L. Taylor  J. White  N. P. Johnston 《Journal of animal physiology and animal nutrition》2014,98(5):908-913

The purpose of this study was to determine the diurnal composition and concentration of volatile fatty acids (VFA) and to determine VFA composition and concentration differences between stomach compartment 1 (C1) and caecum of alpacas fed grass and alfalfa hay. The study was divided into two experiments. In Experiment 1 (EXP 1), 10 male alpacas (3+ years old, 65 kg BW) were divided into two groups, housed in drylot pens, provided ad libitum water and fed alfalfa (AH) or grass hay (GH) for 30 days. The alpacas were slaughtered and the digestive tract collected, divided into sub‐tract sections, weighed and digesta sampled for pH, dry matter (DM) and NDF. Volatile fatty acid composition and concentration were determined on C1 and caecal material. Four adult male (3+ years old, 60 kg BW), C1 fistulated alpacas were housed in metabolism crates and divided into two forage groups for Experiment 2 (EXP 2). Alpacas were fed the forages as in EXP 1. Diurnal C1 VFA samples were drawn at 1, 3, 6, 9, 12, 18 and 24 h post‐feeding. There were no differences between forages for tract weight, C1 and caecum digesta DM or NDF. Differences were noted (p < 0.05) for pH between forages and sub‐tract site. Volatile fatty acids concentrations were different (p < 0.05) for forage and site, and total VFA was higher for AH than GH (110.6 and 79.1 mm ) and C1 than caecum (40.7 and 27.6 mm ). Proportion of VFA was significant (p < 0.05) for forage and site, C1 acetate highest for GH (84.8 vs. 74.0 mm ) and caecum acetate 83.7 and 76.2 mm for GH and AH respectively. These data demonstrate the level of VFA produced in C1 and the caecum of alpacas and the diurnal VFA patterns. Composition of VFA is similar to other ruminant species.  相似文献   

Dietary carbohydrate/lipid ratios and nutritional condition in juvenile southern rock lobster, Jasus edwardsii     

D. J. Johnston  K. A. Calvert  B. J. Crear  C. G. Carter 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):667-682

The availability of formulated diets is considered economically imperative if rock lobster aquaculture, based on the collection and ongrowing of puerulus, is to progress. Central to the development of diets is an understanding of the nutritional requirements of the species. This study determined the effect of different dietary carbohydrate/lipid ratios (17:1, 5:1, 2:1, 0.8:1) on the growth and nutritional condition of juvenile southern rock lobsters, Jasus edwardsii, by measuring standard growth parameters, proximate composition of the whole body and digestive gland, and from a histological investigation of the digestive gland. Four replicate groups of eight lobsters (initial weight 5.08±0.98 g (mean±S.D.)) per diet treatment were held in 50 l tanks, in a recirculating system at 18 °C for 84 days. Maximum growth and the highest levels of lipid and dry matter in digestive glands and whole bodies was found in lobsters fed a diet containing 27% carbohydrate and 13.5% lipid (2:1 ratio) suggesting that of the four experimental diets, this diet provided the best balance of lipid and carbohydrate. Digestive gland histology supported this conclusion and lobsters fed low carbohydrate, high lipid diets were in the best nutritional condition, with high lipid accumulation, and structurally sound epithelial cells. Digestive gland epithelial cells of lobsters fed the high carbohydrate, low lipid diets were compressed, of inconsistent shape and size, with low lipid accumulation. Histology is therefore seen as a feasible method, in addition to growth and proximate composition data, to further examine the effect of diets in nutritional studies of crustaceans.  相似文献   

Agricultural expansion: land use shell game in the U.S. Northern Plains     

Carol A. Johnston 《Landscape Ecology》2014,29(1):81-95

Land area planted to row crops has expanded globally with increased demand for food and biofuels. Agricultural expansion in the Dakota Prairie Pothole Region (DPPR), USA affects a variety of agricultural and non-agricultural land-use types, including grasslands and wetlands that provide critical wildlife habitat and other ecosystem services. The purpose of this study was to quantify recent changes in rural land cover/land use, analyze trends, and interpret results in relation to climate, agronomic practice, and ethanol production. The primary data sources were 1980–2012 statewide cropland data from the U.S. Department of Agriculture (USDA) National Agricultural Statistics Service, and the USDA Cropland Data Layer, produced annually for the DPPR from 2006 through 2012. Area planted to corn or soybean row crops increased, and small grain (e.g., wheat, barley) area decreased significantly over the analysis period. Corn and soybean expanded by 27 % in the DPPR between 2010 and 2012 alone, an areal increase (+15,400 km²) larger than the U.S. state of Connecticut. This expansion displaced primarily small grains and grassland (e.g., pastures, haylands, remnant prairies). Grassland regularly exchanged land with corn and soybean, small grains, and wetlands and water. Corn and soybean had high inter-annual self-replacement values (68–80 %), and continuous corn/soy row cropping was the second most common combination over a three-year period, ranking after continuous grassland. Small grain self-replacement values were only 22–35 %, indicating frequent relocation in the landscape. Temporary gains in wetland and grassland area were attributed to unusually wet climatic conditions and late snowfalls that prevented crop planting. Nearly all of the region’s ethanol refineries were located where corn and soybean crops constituted 50 % or more of the land area. Quantification of grassland losses in the U.S. Northern Plains requires evaluation of all land uses that interact with grasslands, and a longer term perspective that incorporates grassland as part of a normal land-use rotation.  相似文献   

Seawater causes rapid trace metal mobilisation in coastal lowland acid sulfate soils: Implications of sea level rise for water quality     

Vanessa N.L. Wong  Scott G. Johnston  Richard T. Bush  Peter G. Slavich 《Geoderma》2010,160(2):252-263

Coastal floodplains are highly vulnerable to inundation with saline water and the likelihood of inundation will increase with sea level rise. Sediment samples from floodplains containing coastal lowland acid sulfate soils (CLASS) in eastern Australia were subjected to increasing seawater concentration to examine the probable effects of sea level rise on acidity and metal desorption. Ten soils were mixed with synthetic seawater concentrations varying from 0% to 100% at a solid:solution ratio of 1:10 for 4 h. There was a slight decrease in pH (≈ 0.5 units) with increasing seawater concentration following treatment, yet, calculated acidity increased significantly. In most soil treatments, Al was the dominant component of the calculated acidity pool. Al dominated the exchange complex in the CLASS and, correspondingly, was the major metal ion desorbed. In general, concentrations of soluble and exchangeable Al, Fe²⁺, Ni, Mn and Zn in all soil extracts increased with increasing salinity. Increasing trace metal concentrations with increasing seawater concentration is attributed to the combined effects of exchange processes and acidity. The increasing ionic strength of the seawater treatments displaces trace metals and protons adsorbed on sediments, causing an initial decrease in pH. Hydrolysis of desorbed acidic metal cations can further contribute to acidity and increase mobilisation of trace metals. These findings imply that saline inundation of CLASS environments, even by relatively brackish water may cause rapid, shorter-term water quality changes and a pulse release of acidity due to desorption of acidic metal cations.  相似文献