Contribution of individual polyphenolics to total antioxidant capacity of plums   总被引：4，自引：0，他引：4  

Chun OK  Kim DO  Moon HY  Kang HG  Lee CY 《Journal of agricultural and food chemistry》2003,51(25):7240-7245

To study the effect of polyphenolics on antioxidant capacities of plums, the amounts of total phenolics, total flavonoids and individual phenolic compounds, and vitamin C equivalent antioxidant capacity (VCEAC) of eleven plum cultivars was determined. There was a good linear relationship between the amount of total phenolics and total antioxidant capacity (r2 = 0.9887). The amount of total flavonoids and total antioxidant capacity also showed a good correlation (r2 = 0.9653). Although the summation of individual antioxidant capacity was lower than the total antioxidant capacity of plum samples, there was a positive correlation (r2 = 0.9299) of total antioxidant capacity of plum samples with the sum of the VCEACs calculated from individual phenolics. Chlorogenic acids and glycosides of cyanidin, peonidin, and quercetin were major phenolics among eleven plum cultivars. The antioxidant capacity of chlorogenic acids and anthocyanins showed higher correlation (r2) of 0.7751 and 0.6616 to total VCEAC, respectively, than that of quercetin glycosides (r2 = 0.0279). Chlorogenic acids were a major source of antioxidant activity in plums, and the consumption of one serving (100 g) of plums can provide antioxidants equivalent to 144.4-889.6 mg of vitamin C.  相似文献   

QTL identification of yield-related traits and their association with flowering and maturity in soybean     

Weixian Liu  Moon Young Kim  Kyujung Van  Yeong-Ho Lee  Hulin Li  Xianhu Liu  Suk-Ha Lee 《Journal of Crop Science and Biotechnology》2011,14(1):65-70

Two soybean recombinant inbred line populations, Jinpumkong 2 × SS2-2 (J × S) and Iksannamulkong × SS2-2 (I x S) showed population-specific quantitative trait loci (QTLs) for days to flowering (DF) and days to maturity (DM) and these were closely correlated within population. In the present study, we identified QTLs for six yield-related traits with simple sequence repeat markers, and biological correlations between flowering traits and yield-related traits. The yield-related traits included plant height (PH), node numbers of main stem (NNMS), pod numbers per plant (PNPP), seed numbers per pod (SNPP), 100-seed weight (SW), and seed yield per plant (SYPP). Eighteen QTLs for six yield-related traits were detected on nine chromosomes (Chrs), containing four QTLs for PH, two for NNMS, two for PNPP, three for SNPP, five for SW, and two for SYPP. Two highly significant QTLs for PH and NNMS were identified on Chr 6 (LG C2) in both populations where the major flowering gene, E1, and two DF and DM QTLs were located. One other PNPP QTL was also located on this region, explaining 12.9% of phenotypic variation. Other QTLs for yield-related traits showed population-specificity. Two significant SYPP QTLs potentially related with QTLs for SNPP and PNPP were found on the same loci of Chrs 8 (Satt390) and 10 (Sat_108). Also, highly significant positive phenotypic correlations (P < 0.01) were found between DF with PH, NNMS, PNPP, and SYPP in both populations, while flowering was negatively correlated with SNPP and SW in the J × S (P < 0.05) and I × S (P < 0.01) populations. Similar results were also shown between DM and yield-related traits, except for one SW. These QTLs identified may be useful for marker-assisted selection by soybean breeders.  相似文献   

Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst     

Hwang WS  Ryu YJ  Park JH  Park ES  Lee EG  Koo JM  Jeon HY  Lee BC  Kang SK  Kim SJ  Ahn C  Hwang JH  Park KY  Cibelli JB  Moon SY 《Science (New York, N.Y.)》2004,303(5664):1669-1674

Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.  相似文献   

THE DETERMINATION OF THE INTERNAL GASES OF PLANT TISSUES     

Culpepper CW  Moon HH  Lutz JM 《Science (New York, N.Y.)》1936,84(2183):398-400

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G protein signaling from activated rat frizzled-1 to the beta-catenin-Lef-Tcf pathway     

Liu T  DeCostanzo AJ  Liu X  Wang Hy  Hallagan S  Moon RT  Malbon CC 《Science (New York, N.Y.)》2001,292(5522):1718-1722

The frizzled receptors, which mediate development and display seven hydrophobic, membrane-spanning segments, are cell membrane-localized. We constructed a chimeric receptor with the ligand-binding and transmembrane segments from the beta2-adrenergic receptor (beta2AR) and the cytoplasmic domains from rat Frizzled-1 (Rfz1). Stimulation of mouse F9 clones expressing the chimera (beta2AR-Rfz1) with the beta-adrenergic agonist isoproterenol stimulated stabilization of beta-catenin, activation of a beta-catenin-sensitive promoter, and formation of primitive endoderm. The response was blocked by inactivation of pertussis toxin-sensitive, heterotrimeric guanine nucleotide-binding proteins (G proteins) and by depletion of Galphaq and Galphao. Thus, G proteins are elements of Wnt/Frizzled-1 signaling to the beta-catenin-lymphoid-enhancer factor (LEF)-T cell factor (Tcf) pathway.  相似文献   

Slow neutron scattering experiments     

Moon RM 《Science (New York, N.Y.)》1985,230(4723):274-280

Neutron scattering is a versatile technique that has been successfully applied to condensed-matter physics, biology, polymer science, chemistry, and materials science. The United States lost its leadership role in this field to Western Europe about 10 years ago. Recently, a modest investment in the United States in new facilities and a positive attitude on the part of the national laboratories toward outside users have resulted in a dramatic increase in the number of U.S. scientists involved in neutron scattering research. Plans are being made for investments in new and improved facilities that could return the leadership role to the United States.  相似文献   

Effect of wnt-1 and related proteins on gap junctional communication in Xenopus embryos     

Olson D  Christian J  Moon R 《Science (New York, N.Y.)》1991,252(5009):1173-1176

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Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling     

Major MB  Camp ND  Berndt JD  Yi X  Goldenberg SJ  Hubbert C  Biechele TL  Gingras AC  Zheng N  Maccoss MJ  Angers S  Moon RT 《Science (New York, N.Y.)》2007,316(5827):1043-1046

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Metabolism of a new herbicide, [(14)c]pyribenzoxim, in rice     

Chang HR  Keum YS  Koo SJ  Moon JK  Kim K  Kim JH 《Journal of agricultural and food chemistry》2011,59(5):1918-1923

The in vivo metabolism of a new herbicide pyribenzoxim (benzophenone Ο-[2,6-bis(4,6-dimethoxypyrimidin-2-yloxy)benzoyl]oxime) in rice was carried out using container trials. Two radiolabeled forms of [carbonyl-(14)C]pyribenzoxim (P1) and [ring-(14)C(U)]pyribenzoxim (P2) were treated separately as formulations for foliar treatment by single applications of 50 g of active ingredient (ai)/ha at the 4-6 leaves stage. At 0, 7, 30, and 60 days after treatment (DAT), samples of panicle, foliage/rest of plant, and roots were taken for analysis. Upon harvest (120 DAT), rice plants were separated into grain, husk, straw, and root parts. Total radioactive residues (TRRs) at each sampling date were determined to show that the final radioactive residues at harvest were low in grain, husk, straw, and roots, accounting for <17 ppb. The concentration of final residues in the rice plant decreased rapidly, and less than 0.1% of initial TRRs remained at harvest. At 7 DAT, metabolite 1 [M1, 2,6-bis(4,6-dimethoxypyrimidin-2-yloxy)benzoic acid] and two unknown compounds (other-1 and other-2) were detected in foliage extract, accounting for 3.5% TRRs (21.0 ppb), 3.1% TRRs (19.0 ppb), and 9.0% TRRs (54.3 ppb), respectively, while 26.1% of M1 was observed in solvent wash. Any other metabolites were not detected in the plant, including expected metabolite M3 (benzophenone oxime). On the basis of the results obtained, a metabolic pathway of pyribenzoxim in a rice plant was proposed.  相似文献   

Cytotoxicity of gamma-ray in rat immature hippocampal neurons     

Miyoung Yang  Myoung-Sub Song  Sung-Ho Kim  Jong-Choon Kim  Joong-Sun Kim  Taekyun Shin  Changjong Moon 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(3):203-207

This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked γ-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of γ-rays in immature hippocampal cultured neurons may be caused by oxidative stress.  相似文献