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The stabilisation of soil organic matter (SOM) is the result of the simultaneous action of three mechanisms: chemical stabilisation, biochemical stabilisation and physical protection. The objectives of this study were (i) to evaluate carbon-protection mechanisms in different SOM pools in soil aggregates and (ii) to identify the association of Ca2 + with total organic carbon (TOC) under the influence of surface liming in a medium-textured Oxisol in a long-term experiment under no-till system (NTS) in southern Brazil (25° 10′ S, 50° 05′ W). The treatments consisted of application of zero or 6 tons ha? 1 of dolomitic lime on the soil surface in 1993 and a reapplication of zero or 3 tons ha? 1 of dolomitic lime in 2000 to plots with or without previous lime application. Soil samples collected at depths of 0–2.5, 2.5–5, 5–10 and 10–20 cm were separated into seven aggregate classes. In each of these classes, TOC, particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) were analysed. The 8–19 mm sized aggregates from the 0–2.5 cm layer were assessed by energy-dispersive X-ray spectroscopy (EDS) for the elemental analysis of carbon (C) and calcium (Ca). The liming caused an accumulation of TOC in the aggregates, mainly at a depth of 0–2.5 cm. The aggregates from soils treated with lime had a higher mean weight diameter (MWD) that resulted in the accumulation of TOC, especially in the 8–19 mm aggregate class, that was linear and closely related with C input (R2 = 0.99). The proportion of large aggregates in the treatments with lime was closely correlated with the TOC content of the whole sample. The largest dose of lime (9 tons ha? 1) resulted in higher TOC, POC and MAOC values, mainly in the 8–19 mm aggregate class. The elemental analyses for C and Ca revealed similar spectra between them for the surface-liming treatments in the clay fraction found in the centres of the 8–19 mm aggregates. The surface application of lime to NT fields provided greater stability and protection of the intra-aggregate C, presumably due to Ca2 + acting as a cationic bridge between OC and the kaolinite in the clay fraction.  相似文献   
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Rhamdia quelen is the most produced native freshwater fish in fish farms in South Brazil. Until recently, Rhamdia branneri and Rhamdia voulezi were synonyms of R. quelen, and all the species are commercialized as silver catfish (locally called jundiá or bagre sapo) by the aquaculture industry. In fact, because these species are morphologically very similar, interspecific crosses easily might occur in fish farming. We employed standard DNA barcoding to identify jundiá molecular operational taxonomic units (MOTUs) in fish cultivated and commercialized in the industry and in possible escapees in the natural environment in southern Brazil. We analysed 48 individuals from six fish farms and 48 individuals from three rivers (Uruguay, Benedito Novo and Itapocu Rivers) using the mitochondrial cytochrome oxidase subunit I (COI). Four MOTUs were identified based on the estimated optimum threshold (OT = 0.77), and these MOTUs were concordant with Bayesian Inferece (BI) and Neighbour‐Joining (NJ) trees. Our results support the existence of at least three species in our dataset: R. branneri, R. voulezi, and R. quelen 1 and R. quelen 2. The interspecific genetic divergence ranged from 1.1% to 5.1% (mean = 3.5%), and the intraspecific distance ranged from 0% to 1.4% (mean = 0.24%). The presence of cultivated fish in the Uruguay and Benedito Novo Rivers provides evidence of genetic contamination in native populations. These results show the need to regulate aquaculture activities and to characterize the species and commercial lineages of silver catfish that are cultivated in South Brazil.  相似文献   
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Brain disease is an important cause of neurologic deficits in small ruminants, however few MRI features have been described. The aim of this retrospective, case series study was to describe MRI characteristics in a group of small ruminants with confirmed brain disease. A total of nine small ruminants (six sheep and three goats) met inclusion criteria. All had neurologic disorders localized to the brain and histopathologic confirmation. In animals with toxic‐metabolic diseases, there were bilaterally symmetric MRI lesions affecting either the gray matter (one animal with polioencephalomalacia) or the white matter (two animals with enterotoxemia). In animals with suppurative inflammation, asymmetric focal brainstem lesions were present (two animals with listeric encephalitis), or lesions typical of an intra‐axial (one animal) or dural abscess (one animal), respectively. No MRI lesions were detected in one animal with suspected viral cerebellitis and one animal with parasitic migration tracts. No neoplastic or vascular lesions were identified in this case series. Findings from the current study supported the use of MRI for diagnosing brain diseases in small ruminants.  相似文献   
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ABSTRACT The prophylactic efficiency of Milsana against powdery mildew was evaluated on wheat (Triticum aestivum). A single short spraying on 10-day-old plantlets reduced the infection level by 85% and two long sprayings led to the total restriction of the disease. Although microscopic studies showed that Milsana treatments enhance hydrogen peroxide accumulation at the fungal penetration site, biochemical analysis did not allow us to correlate this accumulation with the activation of several enzyme activities involved in active oxygen species (AOS) metabolism. Only lipoxygenase activity, which is involved in both AOS metabolism and lipid peroxidation, showed a 26 to 32% increase 48-h posttreatment in leaves infiltrated with Milsana. This weak effect of Milsana on wheat lipid metabolism was confirmed at the lipid peroxidation level, which surprisingly, was shown to decrease in treated plants. In order to explain the high efficacy of Milsana, the fungistatic effect on conidia germination was also examined. In planta, we showed that a Milsana treatment resulted in a higher proportion of abnormally long appressorial germ tubes, whereas in vitro, it dramatically inhibited fungal conidia germination. The partial activity of Milsana in terms of defense response induction in the wheat/powdery mildew pathosystem and its newly described direct fungistatic activity are discussed.  相似文献   
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The impact of three commercially available nanoparticles (NPs) on trichloroethylene (TCE) adsorption onto granular activated carbon (GAC) was investigated. TCE adsorption isotherm and column breakthrough experiments were conducted in the presence and absence of silicon dioxide, titanium dioxide, and iron oxide nanoparticles. A rapid small-scale column test (RSSCT) was assessed for its ability to predict TCE adsorption in pilot-scale GAC in the presence and absence of NPs. Zeta potential of the three NPs and the GAC were measured. Particle size distribution of the NP dispersions was analyzed as a function of time. The surface area and the pore size distribution of the virgin and the exhausted GAC were obtained along with transmission electron microscopy and Fourier transform infrared spectroscopy analysis. The effect of NPs was found to be a function of their zeta potential, concentration, and particle size distribution. Due to their electrical charge, NPs attached to the GAC and blocked the pores and thus reduced the access to the internal pore structure. However, due to the fast adsorption kinetics of TCE, no effect from the three NPs was observed in the isotherm and kinetic studies. The RSSCT, on the other hand, accurately predicted the pilot-column TCE breakthrough in the presence of NPs.  相似文献   
18.
The study aimed to evaluate the effect of the partial replacement of elephant grass silage with babassu cake (Orbignya speciosa) on the carcass characteristics and meat quality of feedlot lambs. Forty-five castrated male Santa Ines sheep (19.08?±?0.41 kg) approximately 4 months old were distributed in a completely randomized design, with five treatments 0.0, 12.5, 25.0, 37.5 and 50 % (%DM) replacement of babassu cake with silage forming isoproteic diets formulated at a ratio of 40 % roughage to 60 % concentrate. All of the studied animals were slaughtered at the end of the experiment. The liver weights and yields increased with the inclusion of babassu cake. The weight of the shoulder increased from 2.31 to 2.61 kg, while the loin yield decreased from 7.38 to 6.64 % with the inclusion of babassu cake, both linearly. The body length, thoracic perimeter, rump perimeter and carcass compactness index showed high and positive correlations with the hot and cold carcass weights. The myofibrillar fragmentation index decreased linearly as a function of the inclusion level of babassu cake, but other quality variables were not affected. The replacement of up to 50 % of the elephant grass silage with babassu cake in the diet of lambs does not cause negative effects on carcass characteristics or meat quality.  相似文献   
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Understanding why some people establish and maintain effective control of HIV-1 and others do not is a priority in the effort to develop new treatments for HIV/AIDS. Using a whole-genome association strategy, we identified polymorphisms that explain nearly 15% of the variation among individuals in viral load during the asymptomatic set-point period of infection. One of these is found within an endogenous retroviral element and is associated with major histocompatibility allele human leukocyte antigen (HLA)-B*5701, whereas a second is located near the HLA-C gene. An additional analysis of the time to HIV disease progression implicated two genes, one of which encodes an RNA polymerase I subunit. These findings emphasize the importance of studying human genetic variation as a guide to combating infectious agents.  相似文献   
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