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11.
A method is described for determining the artificial sweetener aspartame in beverages and beverage mixes by liquid chromatography. Aspartame is separated on a microC18 column, using a mobile phase of acetic acid, water, and isopropyl alcohol at pH 3.0 and UV detection at 254 nm. Beverages are filtered through 0.45 micron filters and injected directly into the chromatograph. Aspartame is eluted in approximately 7 min. Detection of aspartame is confirmed by a UV scan of the trapped peak. Aspartame is quantitated in the presence of other beverage additives such as saccharin, caffeine, sodium benzoate, artificial colors, and artificial flavors. Results are presented for spiked soda beverages, beverages from fruit-flavored mixes, instant tea, reconstituted presweetened drink mixes, and a powdered tabletop sweetener. 相似文献
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Beckman BW 《Journal of veterinary dentistry》2004,21(3):159-163
This case report describes the use synthetic bone graft particulate and 24% EDTA gel to treat an infrabony defect adjacent to the mandibular right first molar tooth in an American Eskimo dog. Postoperative examination 33-months following surgery showed osseous integration at the infrabony defect and restoration of the periodontal ligament space with a small refractory periodontal pocket. 相似文献
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A cat with pelvic fractures from being hit by a car had voided no urine in 13 hours despite iv administration of fluids. Abdominal radiography had revealed the pelvic fractures and apparently intact urinary bladder. Urine did not flow from a urethral catheter, and contrast radiography revealed the catheter to be extra-urethral within the penis. Intravenous pyelography revealed intact ureters and urinary bladder; celiotomy confirmed intact urinary bladder. Cystotomy and normograde urethral catheterization allowed localization of the ruptured urethra within the deep subcutaneous tissues of the caudal portion of the abdomen. After closure of the cystotomy and celiotomy incisions, perineal urethrostomy was performed. 相似文献
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Standardized evaluation and rating of biofilters: II. Manufacturer''s and user''s perspective 总被引:1,自引:0,他引:1
Douglas G. Drennan II K.C. Hosler Mark Francis Dallas Weaver Ed Aneshansley Gregory Beckman Chandler H. Johnson Chad M. Cristina 《Aquacultural Engineering》2006,34(3):403-416
As manufacturers of biofilters we feel that it is important that our customers have a standardized set of criteria that can be used to compare the many different commercially available biofilters on the market today. The purpose of this paper is to show how a variety of manufacturers size their own filters, establish commonalities between the methods and suggest, to the engineering community, the needs of the industry that are required to develop a uniform sizing criteria. Similarities in sizing methodologies include standard information supplied by the consumer (system volume, feed rate, etc.) as well as those estimated by the manufacturer (TAN production, and hydraulic requirements). Differences in sizing methodology include the use of in situ nitrification and system flushing. From the various examples of biofilter sizing, it is clear that manufacturers use a sizing methodology based on either an areal or volumetric TAN conversion rate (ATR or VTR). As manufacturers, we should be able to publish ATR or VTR for each of three trophic levels (oligotrophic, mesotrophic and eutrophic) with appropriate correction factors for water temperature and salinity. A standardized labeling system that would allow system designers to accurately and rapidly determine which biofilter(s) meet their needs is proposed. We leave it to the engineering community to devise a standardized scheme by which VTR values could be determined to facilitate this comparison. 相似文献
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The objective of this study was to quantify the role of maternal effects on docility in Limousin cattle. Docility scores were obtained at weaning while animals were restrained in a squeeze chute. Scores 1 through 6 represented a docile to aggressive temperament, respectively, and were provided by the North American Limousin Foundation. Observations with unknown age of dam, contemporary groups containing less than 10 observations, contemporary groups with no variation, and single-sire contemporary groups were removed, leaving 21,932 observations. A 2-generation pedigree file compiled from animals with observations contained 49,459 animals. Fixed effects were weaning contemporary group and age of dam (2, > or =3 yr). Six animal models encompassed combinations of random factors: direct genetic, maternal genetic, and maternal permanent environmental effects. The model D was the most basic, containing direct genetic and residual effects, and it resembled the method currently used by the North American Limousin Foundation for genetic evaluation of docility. Maternal genetic or permanent environmental effects were separately added to the model D, denoted as models DM and DC, respectively. Model DMC contained all random factors. Models DM-Zero and DMC-Zero were equivalent to models DM and DMC, respectively, but with zero direct-maternal genetic covariance. Direct heritability estimates were moderate for all models (0.29 +/- 0.02 to 0.38 +/- 0.03). Maternal heritability estimates were low, ranging from 0.01 +/- 0.01 (DM-Zero) to 0.05 +/- 0.02 (DM). Negative direct-maternal genetic correlations of -0.41 +/- 0.09 and -0.55 +/- 0.09 were estimated for models DM and DMC, respectively. The proportion of phenotypic variance accounted for by maternal permanent environmental effects was 0.03 +/- 0.01, 0.04 +/- 0.01, and 0.02 +/- 0.01 for models DC, DMC, and DMC-Zero, respectively. Likelihood ratio tests indicated that model DMC best fit the data. Although maternal genetic and maternal permanent environmental effects were significant, they accounted for only 8% (model DMC) of the phenotypic variance, and a Spearman rank correlation of 0.99 between models D and DMC showed sires did not rank differently with or without inclusion of these effects. Given these results, inclusion of maternal effects to the genetic evaluation of docility in Limousin cattle does not seem warranted. 相似文献
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Chinook salmon emergence phenotypes: Describing the relationships between temperature,emergence timing and condition factor in a reaction norm framework
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Abby E. Fuhrman Donald A. Larsen E. Ashley Steel Graham Young Brian R. Beckman 《Ecology of Freshwater Fish》2018,27(1):350-362
Water temperature can have a profound influence on development and distribution of aquatic species. Salmon are particularly vulnerable to temperature changes because their reproductive and early development life phases are spent in freshwater river systems where temperature fluctuates widely both daily and seasonally. Flow regulation downstream of dams can also cause temperature regime changes, which in turn may spur local adaptation of early life‐history traits. In a common garden laboratory incubation experiment, we exposed spring Chinook salmon (Oncorhynchus tshawytscha) embryos to four temperature regimes: warm stable, cold stable, daily variation and below dam. We found that fry from warmer thermal regimes emerged earlier than those from colder regimes both in terms of calendar date and temperature units and that warmer temperatures caused fry to emerge less developed. There was also a significant effect of family on both emergence timing and development level at emergence. By combining measurements of physiological and behavioural traits at emergence and interpreting them within a reaction norm framework, we can better understand which populations might be more vulnerable to altered thermal regimes. 相似文献