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排序方式: 共有340条查询结果,搜索用时 46 毫秒
71.
Romero C Medina E Vargas J Brenes M De Castro A 《Journal of agricultural and food chemistry》2007,55(3):680-686
Helicobacter pylori is linked to a majority of peptic ulcers and to some types of gastric cancer, and resistance of the microorganism to antibiotic treatment is now found worldwide. Virgin olive oil is an unrefined vegetable oil that contains a significant amount of phenolic compounds. Under simulated conditions, we have demonstrated that these substances can diffuse from the oil into the gastric juice and be stable for hours in this acidic environment. In vitro, they exerted a strong bactericidal activity against eight strains of H. pylori, three of them resistant to some antibiotics. Among the phenolic compounds, the dialdehydic form of decarboxymethyl ligstroside aglycon showed the strongest bactericidal effect at a concentration as low as 1.3 microg/mL. Although the experimental conditions are different from other reported works, this bactericidal concentration is much lower than those found for phenolic compounds from tea, wine, and plant extracts. These results open the possibility of considering virgin olive oil a chemopreventive agent for peptic ulcer or gastric cancer, but this bioactivity should be confirmed in vivo in the future. 相似文献
72.
73.
Affinity purification of copper-chelating peptides from sunflower protein hydrolysates 总被引:1,自引:0,他引:1
Megías C Pedroche J Yust MM Girón-Calle J Alaiz M Millan F Vioque J 《Journal of agricultural and food chemistry》2007,55(16):6509-6514
Copper-chelating peptides were purified from sunflower protein hydrolysates by affinity chromatography using immobilized copper. A variety of protein hydrolysates were obtained by incubation with the proteases Alcalase and Flavourzyme for different periods of time. Chelating activity was indirectly determined by measuring the inhibitory effect of hydrolysates on the oxidation of beta-carotene by copper. Copper-binding peptides purified from the two hydrolysates that inhibited oxidation by copper the most contained 25.4 and 42.0% histidine and inhibited beta-carotene oxidation 8 and 3 times more than the original hydrolysates, which had 2.4 and 2.6% histidine, respectively. Thus, histidine content is not the only factor involved in antioxidant activity, and probably other factors such as peptide size and amino acid sequence are also important. This work shows that affinity chromatography can be used for the purification of copper-chelating peptides and probably other metals of nutritional interest such as calcium, iron, and zinc. In addition to their antioxidant potential, chelating peptides are of nutritional interest because they increase bioavailability of minerals. 相似文献
74.
Aranaz A De Juan L Bezos J Alvarez J Romero B Lozano F Paramio JL López-Sánchez J Mateos A Domínguez L 《Veterinary research》2006,37(4):593-606
The intradermal tuberculin (IDTB) test and the interferon-gamma (IFN-gamma) assay are used worldwide for detection of bovine tuberculosis in cattle, but little is known about the effect of co-infecting agents on the performance of these diagnostic tests. This report describes a field trial conducted in a cattle herd with dual infection (bovine tuberculosis and paratuberculosis) during 3.5 years. It has been based on a strategic approach encompassing serial parallel testing (comparative IDTB test, the IFN-gamma assay and serology of paratuberculosis) that was repeated 8 times over the period, and segregation of animals into two herds. The IDTB test detected 65.2% and the IFN-gamma test detected 69.6% of the Mycobacterium bovis culture-positive cattle. However, the IDTB test performed better during the first part of the trial, while the IFN-gamma test was the only method that detected infected animals during the following three samplings. The number of false positive reactors with the IDTB and/or the IFN-gamma tests was remarkably high compared to other reports, and could be caused by cross-reactivity with M. avium subsp. paratuberculosis. Also, the M. bovis isolates from cattle and wildlife from the same property were characterised using molecular techniques to disclose an epidemiological link. The IDTB test may not be appropriate to eradicate bovine tuberculosis in herds with dual mycobacterial infections. This report highlights the need to use several diagnostic techniques for the accurate detection of M. bovis infected animals in these herds. 相似文献
75.
Two randomized field experiments tested a social-psychological intervention designed to improve minority student performance and increase our understanding of how psychological threat mediates performance in chronically evaluative real-world environments. We expected that the risk of confirming a negative stereotype aimed at one's group could undermine academic performance in minority students by elevating their level of psychological threat. We tested whether such psychological threat could be lessened by having students reaffirm their sense of personal adequacy or "self-integrity." The intervention, a brief in-class writing assignment, significantly improved the grades of African American students and reduced the racial achievement gap by 40%. These results suggest that the racial achievement gap, a major social concern in the United States, could be ameliorated by the use of timely and targeted social-psychological interventions. 相似文献
76.
Jorge Martín-García Agustín Merino Julio Javier Diez 《European Journal of Forest Research》2012,131(4):1185-1198
The area covered by plantations of hybrid poplar in Europe is increasing greatly because of the high profitability of these trees. However, the productivity varies widely depending on nutritional status, and it is therefore essential to identify the limiting nutrients. The aims of this study were (a) to identify the main nutrients limiting the growth and vitality of monoclonal poplar (Populus × euramericana) plantations and (b) to develop a means of early detection of nutrition-related problems in growth, based on visual crown conditions (crown transparency, VCT, and visual crown discoloration, VCD). The study was carried out in one of the most suitable areas for this species in Southern Europe. Thirty-two Populus × euramericana (clone I-214) stands displaying different levels of decline were selected for study, and tree growth, nutritional status (soil properties and foliar nutrient concentrations) and crown conditions were assessed. The stands, which were growing in soils with high contents of coarse fragments, displayed low growth rates, poor crown conditions and deficiencies in Fe, B, N, P, K, and to a lesser extent, Zn and Cu. The deficiencies increased with the age of the stand, presumably because of the higher nutrient demand in older trees. Visual crown conditions were related to growth rates and nutritional status. Predictive models were developed to relate crown conditions to the nutritional status identified by analysis of soil properties and foliar concentrations of nutrients. 相似文献
77.
Lucía DeSoto Jesús Julio Camarero José Miguel Olano Vicente Rozas 《European Journal of Forest Research》2012,131(4):905-917
Geographically structured tree-ring networks are needed to fully understand the spatiotemporal variability in climatic sensitiveness of trees and to study their future responses to global warming. We aim to identify the spatially constrained structure of radial-growth patterns of the Spanish juniper (Juniperus thurifera L.) and to assess whether their climate–growth responses were unstable during the late twentieth century. Tree–ring width chronologies were built for 13?J. thurifera stands in Spain using dendrochronological methods and related to monthly climatic data. Sites were grouped according to their growth patterns using hierarchical cluster analysis. The relationships among geographical, climatic and stand features and their influence on radial growth were evaluated using redundancy analysis. The climate–growth relationships and their temporal stability were assessed using Pearson’s and moving bootstrapped correlations, respectively. Stands formed three geographical groups according to their high-frequency growth variation: North West and Centre, North East and South East. We found that J. thurifera radial-growth patterns depended on geographical and climatic factors, but not on the stand structure, and responded to a northwest–southeast gradient of decreasing rainfall and influence of Atlantic Westerlies and Mediterranean cyclonic activity. The positive response to June precipitation was unstable during the late twentieth century and started earlier in populations from western mesic sites than in eastern xeric sites. This pattern may be related to either decreasing water availability in western than in eastern sites or the resilience of J. thurifera growth from xeric sites in response to the increasing summer aridity. 相似文献
78.
Russell GC Benavides J Grant D Todd H Deane D Percival A Thomson J Connelly M Haig DM 《Veterinary research》2012,43(1):51
ABSTRACT: Protection of cattle from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever (MCF) has been described previously, using an attenuated virus vaccine in an unlicensed adjuvant. The vaccine was hypothesised to induce a protective barrier of virus-neutralising antibody in the oro-nasal region, supported by the observation of high titre neutralising antibodies in nasal secretions of protected animals. Here we describe further analysis of this vaccine strategy, studying the effectiveness of the vaccine formulated with a licensed adjuvant; the duration of immunity induced; and the virus-specific antibody responses in plasma and nasal secretions. The results presented here show that the attenuated AlHV-1 vaccine in a licensed adjuvant protected cattle from fatal intranasal challenge with pathogenic AlHV-1 at three or six months. In addition, animals protected from MCF had significantly higher initial anti-viral antibody titres than animals that succumbed to disease; and these antibody titres remained relatively stable after challenge, while titres in vaccinated animals with MCF increased significantly prior to the onset of clinical disease. These data support the view that a mucosal barrier of neutralising antibody blocks infection of vaccinated animals and suggests that the magnitude of the initial response may correlate with long-term protection. Interestingly, the high titre virus-neutralising antibody responses seen in animals that succumbed to MCF after vaccination were not protective. 相似文献
79.
Onishi JC Park JW Prado J Eades SC Mirza MH Fugaro MN Häggblom MM Reinemeyer CR 《Veterinary microbiology》2012,159(3-4):354-363
Carbohydrate overload models of equine acute laminitis are used to study the development of lameness. It is hypothesized that a diet-induced shift in cecal bacterial communities contributes to the development of the pro-inflammatory state that progresses to laminar failure. It is proposed that vasoactive amines, protease activators and endotoxin, all bacterial derived bioactive metabolites, play a role in disease development. Questions regarding the oral bioavailability of many of the bacterial derived bioactive metabolites remain. This study evaluates the possibility that a carbohydrate-induced overgrowth of potentially pathogenic cecal bacteria occurs and that bacterial translocation contributes toward the development of the pro-inflammatory state. Two groups of mixed-breed horses were used, those with laminitis induced by cornstarch (n=6) or oligofructan (n=6) and non-laminitic controls (n=8). Cecal fluid and tissue homogenates of extra-intestinal sites including the laminae were used to enumerate Gram-negative and -positive bacteria. Horses that developed Obel grade2 lameness, revealed a significant overgrowth of potentially pathogenic Gram-positive and Gram-negative intestinal bacteria within the cecal fluid. Although colonization of extra-intestinal sites with potentially pathogenic bacteria was not detected, results of this study indicate that cecal/colonic lymphadenopathy and eosinophilia develop in horses progressing to lameness. It is hypothesized that the pro-inflammatory state in carbohydrate overload models of equine acute laminitis is driven by an immune response to the rapid overgrowth of Gram-positive and Gram-negative cecal bacterial communities in the gut. Further equine research is indicated to study the immunological response, involving the lymphatic system that develops in the model. 相似文献