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21.
Young-Don Son Da-Jung Kim Ji-Houn Kang Dong-Woo Chang Young-Bae Jin Dong-In Jung Chulhyun Lee Mhan-Pyo Yang Sang-Rae Lee Byeong-Teck Kang 《Irish veterinary journal》2015,68(1)
A 16-year-old, castrated, male English cocker spaniel dog was presented due to generalized alopecia. Routine clinical pathology, endocrine and abdominal ultrasonography results were consistent with a diagnosis of pituitary-dependent hyperadrenocorticism. The adenohypophyseal lesion was clearly visualized on both 3 T and 7 T magnetic resonance imaging (MRI) of the pituitary gland. Although biochemical and MRI findings were consistent with a functional pituitary microtumor, a pituitary lesion was not detected using 18F-fluorodeoxyglucose positron emission tomography (FDG-PET). This report firstly describes the application of high-resolution FDG-PET to a spontaneous pituitary microtumor in a dog. 相似文献
22.
Hyun-Jung Suh Dai Jung Chung A-Jin Lee Hyo-Jin Chung Dae-Hyun Kim Ki-Dong Eom Sun Hee Do Hwi-Yool Kim 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(10):1025-1028
A 10-year-old Maltese dog was presented with a firm mass on the left side of his neck. Physical examination confirmed a firm mass in the left and a submandibular swelling in the right cervical region. Sialolithiasis and associated sialocele in both mandibular salivary glands were suspected and bilateral sialoadenectomy was performed. The stones were identified as non-mineral sialoliths. 相似文献
23.
Jong-Hyun Moon Hae-Won Jung Hee-Chun Lee Joon-Hyeok Jeon Na-Hyun Kim Jung-Hyang Sur Jeongim Ha Dong-In Jung 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(2):203-211
In the present study, the use of dogs with experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs were included in this study. Canine forebrain tissues (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund''s complete adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra. Under sedation, each dog was injected subcutaneously with canine brain homogenate at four sites: two in the inguinal and two in the axillary regions. A second injection (booster) was administered to all the dogs using the same procedure 7 days after the first injection. Clinical assessment, magnetic resonance imaging, cerebrospinal fluid analyses, necropsies, and histopathological and immunohistochemical examinations were performed for the dogs with EAE. Out of the 12 animals, seven (58%) developed clinically manifest EAE at various times after immunization. Characteristics of canine EAE models were very similar to canine NE, suggesting that canine EAE can be a disease model for NE in dogs. 相似文献
24.
In Su Cheon Sung-Moo Park Hye Jin Lee Ji Eun Hong Sang Yun Ji Byoung-Shik Shim Kwang Ho Kim Pil Seung Heo Yoo Yong Kim Hyun Jung Jung Hakhyun Ka Seung Hyun Han Manki Song Cheol-Heui Yun 《Research in veterinary science》2014
In human or mouse, mature T cells express either CD4 or CD8, resulting in different functions in the periphery. Interestingly, porcine CD4 and CD8 double positive (DP) T cells are present in the blood, and their proportions change from youth to adulthood. However, the features of these cells in swine are poorly understood. We investigated the fate of porcine peripheral T cells based on their functional characteristics, including proliferation and the expression of CD4 and CD8 co-receptors. The results showed that all the populations changed their CD8 expression in a time-dependent manner and porcine T cells had different proliferative pattern from human T cells. The results further revealed that Th2 cytokines were increased later in porcine T cells compared to human T cells upon stimulation with IL-2 + PMA. Collectively, we found that the fate of porcine peripheral T cells is different from that of human T cells, and the changes occur in a time- and stimulation-dependent manner. 相似文献
25.
Sung-Il Kang Sang-Eun Lee Ji-Yeon Kim Kichan Lee Jong-Wan Kim Hyang-Keun Lee So-Ra Sung Young-Ran Heo Suk Chan Jung Moon Her 《Comparative immunology, microbiology and infectious diseases》2014
Brucellosis is a zoonotic disease that is transmitted from animals to humans, and the development of a rapid, accurate, and widely available identification method is essential for diagnosing this disease. In this study, we developed a new Brucella canis species-specific (BcSS) PCR assay and evaluated its specificity and sensitivity. A specific PCR primer set was designed based on the BCAN_B0548-0549 region in chromosome II of B. canis. The PCR detection for B. canis included amplification of a 300-bp product that is, not found on other Brucella species or, genetically or serologically related bacteria. The detection limit of BcSS-PCR assay was 6 pg/μl by DNA dilution, or 3 × 103 colony-forming units (CFU) in the buffy coats separated from whole blood experimentally inoculated with B. canis. Using the buffy coat in this PCR assay resulted in approximately 100-times higher sensitivity for B. canis as compared to detect directly from whole blood. This is the first report of a species-specific PCR assay to detect B. canis, and the new assay will provide a valuable tool for the diagnosis of B. canis infection. 相似文献
26.
27.
Ji Hyeon Ahn Joon Ha Park In Hye Kim Jae-Chul Lee Bing Chun Yan Min Sik Yong Choong Hyun Lee Jung Hoon Choi Ki-Yeon Yoo In Koo Hwang Seung Myung Moon Hyung-Cheul Shin Moo-Ho Won 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(3):335-342
Melatonin affects diverse physiological functions through its receptor and plays an important role in the central nervous system. In the present study, we compared immunoreactivity patterns of arylalkylamine N-acetyltransferase (AANAT), an enzyme essential for melatonin synthesis, and melatonin receptor type 1B (MT2) in the spinal cord of young adult (2~3 years) and aged (10~12 years) beagle dogs using immunohistochemistry and Western blotting. AANAT-specific immunoreactivity was observed in the nuclei of spinal neurons, and was significantly increased in aged dog spinal neurons compared to young adult spinal neurons. MT2-specific immunoreactivity was found in the cytoplasm of spinal neurons, and was predominantly increased in the margin of the neuron cytoplasm in aged spinal cord compared to that in the young adult dogs. These increased levels of AANAT and MT2 immunoreactivity in aged spinal cord might be a feature of normal aging and associated with a feedback mechanism that compensates for decreased production of melatonin during aging. 相似文献
28.
Seong Bin Park Kyoung Kwon In Seok Cha Ho Bin Jang Seong Won Nho Fernand F. Fagutao Young Kyu Kim Jong Earn Yu Tae Sung Jung 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(1):163-166
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea. 相似文献
29.
Dae Young Yoo Woosuk Kim Dae Won Kim Sung Min Nam Hyo Young Jung Jong Whi Kim Choong Hyun Lee Jung Hoon Choi Moo-Ho Won Yeo Sung Yoon In Koo Hwang 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(1):27-33
In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus. 相似文献
30.
Accumulation of organotin compounds and marine birnavirus detection in Korean ascidians 总被引:1,自引:0,他引:1
Kaoru Azumi Shinji Nakamura Shin-Ichi Kitamura Sung-Ju Jung Keisuke Kanehira Hisato Iwata Shinsuke Tanabe Satoru Suzuki 《Fisheries Science》2007,73(2):263-269
ABSTRACT: Recently, a serious disease spread extensively in aquaculture sites of the ascidian Halocynthia roretzi in Korea. To understand circumstances of ascidians in Korean aquaculture sites, residue levels of organotin compounds were analyzed, and detection of a marine birnavirus (MABV) in tissues of H. roretzi was attempted. Korean H. roretzi showed high concentrations of butyltins (mono, di, and tributyltins), especially in the gill, hepatopancreas, and digestive tract. However, there was no significant difference in the residues of butyltins in the hepatopancreas between diseased and non-diseased ascidians. The positive rate of MABV detection was high in the hepatopancreas, but also no significant difference was observed between diseased and non-diseased individuals. These observations suggest that an accumulation of tributyltin and a latency of MABV in H. roretzi tissues does not directly relate to the occurrence of the disease. 相似文献